RLG00000005877

4Fe-4S single cluster domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0002219
Physical Location & Seq
Reverse (-)
2508625 .. 2514095
5471 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005877

Sequence Viewer

Length: 1299 bp
ATGGCGGCTATGGCGAACGACTTTCAGGATCGCCGGAATTTGAAGCTCGTTCACGATAACGTTCACGGCATCGTCTACCTCGAGCCGCTCTCCTTGAATTTTATTGACACTGAACAGTTTCAGAGGCTTCGTGATCTGAAGCAACTTGGTCTGACCAGCATGGTTTACCCTGGAGCTGCACATTCTAGATTTGAGCATTCACTTGGGGTGTATTGGCTTGCTGGTGAAGCTATCAATAAAATCAAAACTAACCAAGGCGAGGAGCTTGGAATTGACAGCTTTGATGTGAGAACAGTAAAACTTGCTGGACTACTGCATGATATTGGCCATGGGCCTTTCAGCCACATGTTTGAGAGCGCGTTTCTTCCTCGGGTTCATTCTGGTGACAAATGGTCCCATGAGGATATGTCTGTGAAGATGATTGACTACATTGTCGACGAGCACCACATTGACATTGATTCTGAAGAGCTAAAGAGAGTGAAGGACCTGATCCTGGCTAGCTCTAAACATGTGGCACAGAATGCTGCACCAGAAAAGCGGTTCTTGTATGATATTGTTGCTAATGGACGCACTGGAATAGATGTTGACAAGTTTGATTACATTGAACGTGACTCCCGTGCCTGTGGCATAGGCTGTAATTTTCACTTTGAGAGGCTGATGGAAACTATGCGAGTTATGGAGGATGAGATATGTTACCGTGCTAGTGAATATCTGACAATCAACGAGCTGTTTATGACTCGGGCTAATCTGCATCGGACTGTCTATACGCATGCAAAAGTGACGGCTATTGAACTCATGGCTGTTGATGCTCTGTGCAAAGCAAATGCTGAACTTGGGATTGCAGACTCCATTCACAAACCATCTGAATTCTGGAAGTTCTGCAATCAGTTCACTGTTCCAAAGGACAGACTACATAACTTCAACGATATTACAGCCAAAGATATAATTTGTTCTCAGAAAGCTGGCAATGTTACGCTACAGGAGGAAGATGTGGTCGTGATCACTGTTAAAATTGATTTGACTAATGACAGGAACAGCCCTCTTGAAAGAGTCCATTTCTTCAATGACTATGAGAGTACTGATAAATTCCGAATCCAAGTTGATCGAGTAAGTCACTTGCTTCCTGCATTTTATCAAGACAAGATAGTCAGGGTCTACTCCACGAAGCCGAACCTGGTTGCAGCAGTTGCAGAGGCTTTTGAGAATTATCAGCTGAAGACATATGGGAGGATAGTACAAGTACATTCAACTCCGGAGAAAAAGAAAAAAAGAACTGCACGAAGCTTAGCGCTGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 HD Radical_SAM

Protein Analysis

433

Amino Acids

49.57

Weight (kDa)

6.21

Isoelectric Point (pI)

29.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HD PF01966 63 - 154 4.7e-11 HD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 431, 1145
AccBSI CCGCTC 1 cut(s) 88
AccI GTMKAC 3 cut(s) 75, 435, 1155
AccII CGCG 1 cut(s) 359
AccIII TCCGGA 1 cut(s) 1252
AciI CCGC 3 cut(s) 5, 86, 538
AclI AACGTT 1 cut(s) 60
AclWI GGATC 2 cut(s) 36, 484
AcoI YGGCCR 1 cut(s) 325
AcsI RAATTY 4 cut(s) 37, 97, 866, 1085
AcuI CTGAAG 3 cut(s) 158, 483, 1235
AfaI GTAC 3 cut(s) 1078, 1236, 1242
AfeI AGCGCT 1 cut(s) 1290
AfiI CCNNNNNNNGG 2 cut(s) 259, 493
AflIII ACRYGT 2 cut(s) 345, 508
AgsI TTSAA 8 cut(s) 43, 97, 605, 791, 922, 1046, 1063, 1248
AhdI GACNNNNNGTC 1 cut(s) 391
AjnI CCWGG 3 cut(s) 169, 492, 1173
Alw21I GWGCWC 1 cut(s) 444
AlwI GGATC 2 cut(s) 36, 484
Ama87I CYCGRG 3 cut(s) 80, 369, 738
Aor13HI TCCGGA 1 cut(s) 1252
Aor51HI AGCGCT 1 cut(s) 1290
AoxI GGCC 2 cut(s) 325, 332
ApeKI GCWGC 4 cut(s) 176, 524, 1181, 1291
ApoI RAATTY 4 cut(s) 37, 97, 866, 1085
Asp700I GAANNNNTTC 1 cut(s) 117
AspLEI GCGC 2 cut(s) 359, 1291
AspS9I GGNCC 3 cut(s) 332, 393, 484
AsuHPI GGTGA 2 cut(s) 236, 395
AsuNHI GCTAGC 1 cut(s) 497
AvaI CYCGRG 3 cut(s) 80, 369, 738
AvaII GGWCC 2 cut(s) 393, 484
BalI TGGCCA 1 cut(s) 327
BbsI GAAGAC 1 cut(s) 1223
Bbv12I GWGCWC 1 cut(s) 444
BbvI GCAGC 4 cut(s) 163, 511, 1193, 1278
BccI CCATC 2 cut(s) 652, 868
BceAI ACGGC 2 cut(s) 82, 798
BciT130I CCWGG 3 cut(s) 171, 494, 1175
BclI TGATCA 1 cut(s) 999
BfaI CTAG 3 cut(s) 186, 498, 702
BfmI CTRYAG 2 cut(s) 977, 1292
BfoI RGCGCY 1 cut(s) 1292
BisI GCNGC 6 cut(s) 6, 86, 177, 525, 1182, 1292
BlpI GCTNAGC 1 cut(s) 1285
BlsI GCNGC 6 cut(s) 7, 87, 178, 526, 1183, 1293
BmcAI AGTACT 1 cut(s) 1078
Bme1390I CCNGG 3 cut(s) 171, 494, 1175
Bme18I GGWCC 2 cut(s) 393, 484
BmeRI GACNNNNNGTC 1 cut(s) 391
BmeT110I CYCGRG 3 cut(s) 80, 369, 738
BmgT120I GGNCC 3 cut(s) 332, 393, 484
BmiI GGNNCC 1 cut(s) 395
BmrFI CCNGG 3 cut(s) 171, 494, 1175
BmsI GCATC 3 cut(s) 78, 760, 796
BmtI GCTAGC 1 cut(s) 501
BpiI GAAGAC 1 cut(s) 1223
BplI GAGNNNNNCTC 2 cut(s) 74, 106
BpmI CTGGAG 1 cut(s) 192
Bpu1102I GCTNAGC 1 cut(s) 1285
BsaJI CCNNGG 4 cut(s) 169, 253, 328, 368
BsaWI WCCGGW 1 cut(s) 1252
Bsc4I CCNNNNNNNGG 2 cut(s) 259, 493
Bse1I ACTGG 1 cut(s) 577
Bse3DI GCAATG 1 cut(s) 973
BseAI TCCGGA 1 cut(s) 1252
BseBI CCWGG 3 cut(s) 171, 494, 1175
BseDI CCNNGG 4 cut(s) 169, 253, 328, 368
BseGI GGATG 1 cut(s) 688
BseLI CCNNNNNNNGG 2 cut(s) 259, 493
BseMI GCAATG 1 cut(s) 973
BseMII CTCAG 1 cut(s) 968
BseNI ACTGG 1 cut(s) 577
BseRI GAGGAG 1 cut(s) 275
BseXI GCAGC 4 cut(s) 163, 511, 1193, 1278
BsgI GTGCAG 3 cut(s) 162, 510, 1260
Bsh1236I CGCG 1 cut(s) 359
BshFI GGCC 2 cut(s) 327, 334
BsiHKAI GWGCWC 1 cut(s) 444
BsiHKCI CYCGRG 3 cut(s) 80, 369, 738
BsiSI CCGG 2 cut(s) 34, 1253
BslFI GGGAC 1 cut(s) 379
BslI CCNNNNNNNGG 2 cut(s) 259, 493
BsmFI GGGAC 1 cut(s) 379
BsmI GAATGC 2 cut(s) 196, 526
BsnI GGCC 2 cut(s) 327, 334
BsoBI CYCGRG 3 cut(s) 80, 369, 738
Bsp1286I GDGCHC 1 cut(s) 444
Bsp13I TCCGGA 1 cut(s) 1252
Bsp143I GATC 5 cut(s) 28, 133, 489, 999, 1102
Bsp1720I GCTNAGC 1 cut(s) 1285
Bsp19I CCATGG 1 cut(s) 328
BspACI CCGC 3 cut(s) 5, 86, 538
BspANI GGCC 2 cut(s) 327, 334
BspCNI CTCAG 1 cut(s) 967
BspEI TCCGGA 1 cut(s) 1252
BspFNI CGCG 1 cut(s) 359
BspLI GGNNCC 1 cut(s) 395
BspMAI CTGCAG 1 cut(s) 1296
BspOI GCTAGC 1 cut(s) 501
BspPI GGATC 2 cut(s) 36, 484
BspQI GCTCTTC 1 cut(s) 459
BsrBI CCGCTC 1 cut(s) 88
BsrDI GCAATG 1 cut(s) 973
BsrI ACTGG 1 cut(s) 577
BssECI CCNNGG 4 cut(s) 169, 253, 328, 368
BssMI GATC 5 cut(s) 28, 133, 489, 999, 1102
BssT1I CCWWGG 2 cut(s) 253, 328
Bst2UI CCWGG 3 cut(s) 171, 494, 1175
Bst4CI ACNGT 6 cut(s) 117, 295, 698, 760, 895, 1006
Bst6I CTCTTC 1 cut(s) 459
BstAPI GCANNNNNTGC 2 cut(s) 521, 1187
BstC8I GCNNGC 4 cut(s) 219, 499, 771, 964
BstDEI CTNAG 2 cut(s) 954, 1285
BstDSI CCRYGG 1 cut(s) 328
BstF5I GGATG 1 cut(s) 688
BstFNI CGCG 1 cut(s) 359
BstH2I RGCGCY 1 cut(s) 1292
BstHHI GCGC 2 cut(s) 359, 1291
BstKTI GATC 5 cut(s) 31, 136, 492, 1002, 1105
BstMBI GATC 5 cut(s) 28, 133, 489, 999, 1102
BstMWI GCNNNNNNNGC 5 cut(s) 11, 227, 521, 806, 1187
BstNI CCWGG 3 cut(s) 171, 494, 1175
BstNSI RCATGY 3 cut(s) 349, 512, 773
BstSCI CCNGG 3 cut(s) 169, 492, 1173
BstSFI CTRYAG 2 cut(s) 977, 1292
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 4 cut(s) 163, 511, 1193, 1278
BstV2I GAAGAC 1 cut(s) 1223
BsuRI GGCC 2 cut(s) 327, 334
BtgI CCRYGG 1 cut(s) 328
BtsCI GGATG 1 cut(s) 688
BtsIMutI CAGTG 4 cut(s) 108, 570, 891, 1002
Cac8I GCNNGC 4 cut(s) 219, 499, 771, 964
CfoI GCGC 2 cut(s) 359, 1291
Cfr13I GGNCC 3 cut(s) 332, 393, 484
CseI GACGC 1 cut(s) 576
CsiI ACCWGGT 1 cut(s) 1173
Csp6I GTAC 3 cut(s) 1077, 1235, 1241
CviAII CATG 8 cut(s) 160, 317, 329, 346, 398, 509, 770, 796
CviQI GTAC 3 cut(s) 1077, 1235, 1241
DdeI CTNAG 2 cut(s) 954, 1285
DpnI GATC 5 cut(s) 30, 135, 491, 1001, 1104
DpnII GATC 5 cut(s) 28, 133, 489, 999, 1102
DrdI GACNNNNNNGTC 2 cut(s) 431, 1145
DriI GACNNNNNGTC 1 cut(s) 391
DseDI GACNNNNNNGTC 2 cut(s) 431, 1145
EaeI YGGCCR 1 cut(s) 325
Eam1104I CTCTTC 1 cut(s) 459
Eam1105I GACNNNNNGTC 1 cut(s) 391
EarI CTCTTC 1 cut(s) 459
Eco130I CCWWGG 2 cut(s) 253, 328
Eco47I GGWCC 2 cut(s) 393, 484
Eco47III AGCGCT 1 cut(s) 1290
Eco57I CTGAAG 3 cut(s) 158, 483, 1235
Eco88I CYCGRG 3 cut(s) 80, 369, 738
EcoO109I RGGNCCY 1 cut(s) 484
EcoRI GAATTC 1 cut(s) 866
EcoRII CCWGG 3 cut(s) 169, 492, 1173
EcoT14I CCWWGG 2 cut(s) 253, 328
ErhI CCWWGG 2 cut(s) 253, 328
FaeI CATG 8 cut(s) 163, 320, 332, 349, 401, 512, 773, 799
FalI AAGNNNNNCTT 2 cut(s) 527, 559
FaqI GGGAC 1 cut(s) 379
FatI CATG 8 cut(s) 159, 316, 328, 345, 397, 508, 769, 795
FauNDI CATATG 1 cut(s) 1222
FbaI TGATCA 1 cut(s) 999
FblI GTMKAC 3 cut(s) 75, 435, 1155
Fnu4HI GCNGC 6 cut(s) 6, 86, 177, 525, 1182, 1292
FokI GGATG 1 cut(s) 695
Fsp4HI GCNGC 6 cut(s) 6, 86, 177, 525, 1182, 1292
FspBI CTAG 3 cut(s) 186, 498, 702
GlaI GCGC 2 cut(s) 358, 1290
GluI GCNGC 6 cut(s) 6, 86, 177, 525, 1182, 1292
GsuI CTGGAG 1 cut(s) 192
HaeII RGCGCY 1 cut(s) 1292
HaeIII GGCC 2 cut(s) 327, 334
HapII CCGG 2 cut(s) 34, 1253
HgaI GACGC 1 cut(s) 576
HhaI GCGC 2 cut(s) 359, 1291
Hin1II CATG 8 cut(s) 163, 320, 332, 349, 401, 512, 773, 799
Hin6I GCGC 2 cut(s) 357, 1289
HinP1I GCGC 2 cut(s) 357, 1289
HincII GTYRAC 2 cut(s) 436, 586
HindII GTYRAC 2 cut(s) 436, 586
HindIII AAGCTT 1 cut(s) 1282
HinfI GANTC 6 cut(s) 458, 611, 736, 845, 1050, 1092
HpaII CCGG 2 cut(s) 34, 1253
HphI GGTGA 2 cut(s) 236, 395
Hpy166II GTNNAC 8 cut(s) 52, 64, 76, 166, 436, 586, 891, 1156
Hpy188I TCNGA 9 cut(s) 123, 138, 153, 463, 714, 756, 865, 957, 1091
Hpy188III TCNNGA 9 cut(s) 26, 53, 131, 186, 871, 997, 1043, 1136, 1253
Hpy8I GTNNAC 8 cut(s) 52, 64, 76, 166, 436, 586, 891, 1156
Hpy99I CGWCG 1 cut(s) 440
HpyAV CCTTC 1 cut(s) 475
HpyCH4III ACNGT 6 cut(s) 117, 295, 698, 760, 895, 1006
HpyCH4IV ACGT 2 cut(s) 60, 607
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 227, 521, 806, 1187
HpyF3I CTNAG 2 cut(s) 954, 1285
HpySE526I ACGT 2 cut(s) 60, 607
Hsp92II CATG 8 cut(s) 163, 320, 332, 349, 401, 512, 773, 799
HspAI GCGC 2 cut(s) 357, 1289
Kpn2I TCCGGA 1 cut(s) 1252
Ksp22I TGATCA 1 cut(s) 999
Kzo9I GATC 5 cut(s) 28, 133, 489, 999, 1102
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 2 cut(s) 173, 262
Lsp1109I GCAGC 4 cut(s) 163, 511, 1193, 1278
LweI GCATC 3 cut(s) 78, 760, 796
MabI ACCWGGT 1 cut(s) 1173
MaeI CTAG 3 cut(s) 186, 498, 702
MaeII ACGT 2 cut(s) 60, 607
MaeIII GTNAC 6 cut(s) 383, 608, 692, 778, 970, 1112
MalI GATC 5 cut(s) 30, 135, 491, 1001, 1104
MbiI CCGCTC 1 cut(s) 88
MboI GATC 5 cut(s) 28, 133, 489, 999, 1102
MboII GAAGA 6 cut(s) 356, 427, 476, 998, 1051, 1228
MhlI GDGCHC 1 cut(s) 444
MlsI TGGCCA 1 cut(s) 327
MluCI AATT 9 cut(s) 37, 97, 270, 637, 866, 945, 1011, 1085, 1204
MluNI TGGCCA 1 cut(s) 327
MlyI GAGTC 4 cut(s) 605, 730, 839, 1059
Mox20I TGGCCA 1 cut(s) 327
MroI TCCGGA 1 cut(s) 1252
MroXI GAANNNNTTC 1 cut(s) 117
MscI TGGCCA 1 cut(s) 327
MseI TTAA 1 cut(s) 1008
MslI CAYNNNNRTG 1 cut(s) 381
Msp20I TGGCCA 1 cut(s) 327
MspA1I CMGCKG 1 cut(s) 1213
MspI CCGG 2 cut(s) 34, 1253
MspR9I CCNGG 3 cut(s) 171, 494, 1175
Mva1269I GAATGC 2 cut(s) 196, 526
MvaI CCWGG 3 cut(s) 171, 494, 1175
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 5 cut(s) 11, 227, 521, 806, 1187
NcoI CCATGG 1 cut(s) 328
NdeI CATATG 1 cut(s) 1222
NdeII GATC 5 cut(s) 28, 133, 489, 999, 1102
NheI GCTAGC 1 cut(s) 497
NlaIII CATG 8 cut(s) 163, 320, 332, 349, 401, 512, 773, 799
NlaIV GGNNCC 1 cut(s) 395
NmuCI GTSAC 4 cut(s) 383, 608, 778, 1112
NspI RCATGY 3 cut(s) 349, 512, 773
PaeI GCATGC 1 cut(s) 773
PaeR7I CTCGAG 1 cut(s) 80
PciI ACATGT 2 cut(s) 345, 508
PciSI GCTCTTC 1 cut(s) 459
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 613
PctI GAATGC 2 cut(s) 196, 526
PdmI GAANNNNTTC 1 cut(s) 117
PfeI GAWTC 2 cut(s) 458, 1092
PkrI GCNGC 6 cut(s) 7, 87, 178, 526, 1183, 1293
PleI GAGTC 4 cut(s) 605, 730, 839, 1058
PpsI GAGTC 4 cut(s) 605, 730, 839, 1058
PpuMI RGGWCCY 1 cut(s) 484
PscI ACATGT 2 cut(s) 345, 508
Psp1406I AACGTT 1 cut(s) 60
Psp5II RGGWCCY 1 cut(s) 484
Psp6I CCWGG 3 cut(s) 169, 492, 1173
PspGI CCWGG 3 cut(s) 169, 492, 1173
PspN4I GGNNCC 1 cut(s) 395
PspPI GGNCC 3 cut(s) 332, 393, 484
PspPPI RGGWCCY 1 cut(s) 484
PspXI VCTCGAGB 1 cut(s) 80
PstI CTGCAG 1 cut(s) 1296
PvuII CAGCTG 1 cut(s) 1213
RsaI GTAC 3 cut(s) 1078, 1236, 1242
RsaNI GTAC 3 cut(s) 1077, 1235, 1241
RseI CAYNNNNRTG 1 cut(s) 381
SalI GTCGAC 1 cut(s) 434
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 1 cut(s) 1008
SatI GCNGC 6 cut(s) 6, 86, 177, 525, 1182, 1292
Sau3AI GATC 5 cut(s) 28, 133, 489, 999, 1102
Sau96I GGNCC 3 cut(s) 332, 393, 484
ScaI AGTACT 1 cut(s) 1078
SchI GAGTC 4 cut(s) 605, 730, 839, 1059
ScrFI CCNGG 3 cut(s) 171, 494, 1175
SduI GDGCHC 1 cut(s) 444
SexAI ACCWGGT 1 cut(s) 1173
SfaNI GCATC 3 cut(s) 78, 760, 796
SfcI CTRYAG 2 cut(s) 977, 1292
Sfr274I CTCGAG 1 cut(s) 80
SinI GGWCC 2 cut(s) 393, 484
SlaI CTCGAG 1 cut(s) 80
SmiMI CAYNNNNRTG 1 cut(s) 381
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
SphI GCATGC 1 cut(s) 773
Sse9I AATT 9 cut(s) 37, 97, 270, 637, 866, 945, 1011, 1085, 1204
SsiI CCGC 3 cut(s) 5, 86, 538
SspMI CTAG 3 cut(s) 186, 498, 702
StyD4I CCNGG 3 cut(s) 169, 492, 1173
StyI CCWWGG 2 cut(s) 253, 328
TaaI ACNGT 6 cut(s) 117, 295, 698, 760, 895, 1006
TaiI ACGT 2 cut(s) 63, 610
TaqI TCGA 3 cut(s) 81, 435, 1105
TasI AATT 9 cut(s) 37, 97, 270, 637, 866, 945, 1011, 1085, 1204
TatI WGTACW 3 cut(s) 1076, 1234, 1240
TauI GCSGC 2 cut(s) 8, 88
TfiI GAWTC 2 cut(s) 458, 1092
Tru1I TTAA 1 cut(s) 1008
Tru9I TTAA 1 cut(s) 1008
TscAI CASTG 4 cut(s) 115, 577, 898, 1009
TseFI GTSAC 4 cut(s) 383, 608, 778, 1112
TseI GCWGC 4 cut(s) 176, 524, 1181, 1291
Tsp45I GTSAC 4 cut(s) 383, 608, 778, 1112
TspDTI ATGAA 1 cut(s) 365
TspRI CASTG 4 cut(s) 115, 577, 898, 1009
VpaK11BI GGWCC 2 cut(s) 393, 484
XapI RAATTY 4 cut(s) 37, 97, 866, 1085
XbaI TCTAGA 1 cut(s) 185
XceI RCATGY 3 cut(s) 349, 512, 773
XcmI CCANNNNNNNNNTGG 1 cut(s) 867
XhoI CTCGAG 1 cut(s) 80
XmiI GTMKAC 3 cut(s) 75, 435, 1155
XmnI GAANNNNTTC 1 cut(s) 117
XspI CTAG 3 cut(s) 186, 498, 702
ZrmI AGTACT 1 cut(s) 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.