Rroxscaffold_5G00384470

4Fe-4S single cluster domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002219
Physical Location & Seq
Forward (+)
63840576 .. 63846640
6065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00384470.1

Sequence Viewer

Length: 939 bp
ATGTCTGTGAAGATGATTGACTACATTGTCGACGAGCACCACATTGACATTGATTCTGAAGAGCTAAAGAGAGTGAAGGACCTGATCCTGGCTAGCTCTAAACATGTGGCACAGAATGCTGCACCAGAAAAGCAGTTCTTGTATGATATTGTTGCTAATGGACGCACTGGAATAGATGTTGACAAGTTTGATTACATTGAACGTGACTCCCGTGCCTGTGGCATAGGCTGTAATTTTCACTTTGAGAGGCTGATGGAAACTATGCGAGTTATGGAGGATGAGATATGTTACCGTGCTAATGAATATCTGACAATCAACAAGCTGTTTATGACTCGGGCTAATCTGCATCGGACTGTCTATACGCATGCAAAAGTGAAGGCTATTGAACTCATGGCTGTTGATGCTCTGTGCAAATCAAATGCTGAACTTGGGATTGCAGACTCCATTCACAAACCATCTGAATTCTGGAAGTTAGATGATTCAATTCTGAAAAAAATTGAAACGTCTGACAACCCTGAACTGAAGGAGGCAAAGGATTTGATCTTACGCATTCGAAGAAGGGATCTCTACCAGAAAGCTGGCAATGTTACTCTACAGGAGGAAGATGTGGTTGTGATCACTGTTAAAATTGATTTGACTAATGACAGGAACAGCCCTCTTGAAAGCCTCAATACTGTGACTGCAAATGCTATGATTGTATCTTCAGACTATGAGAGTACTGATAAATTCCGAATCCAAGTTGACCGCGTAAGTCACTTGCTTCCTGCATTTTATCAAGACAAGATAGTCAGGGTCTACTCCAAGAAGTCGAACCTGGTTGCAGCAGTTGCAGATGCTTTTGAGAATTATCAGCGGAAGACATATGGGAGGATATTACAAGTACATTCAACTCCGGAGAAAAAGAAGAAAAGAACTGCACGAAGCTTAGCGCTGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 HD Radical_SAM

Protein Analysis

312

Amino Acids

35.89

Weight (kDa)

7.12

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HD_assoc_2 PF19276 81 - 233 4.7e-08 HD associated region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 26, 785
AccI GTMKAC 2 cut(s) 30, 795
AccII CGCG 1 cut(s) 747
AccIII TCCGGA 1 cut(s) 892
AciI CCGC 2 cut(s) 745, 853
AclWI GGATC 2 cut(s) 79, 570
AcsI RAATTY 2 cut(s) 461, 725
AcuI CTGAAG 3 cut(s) 78, 542, 687
AfaI GTAC 2 cut(s) 718, 882
AfeI AGCGCT 1 cut(s) 930
AfiI CCNNNNNNNGG 1 cut(s) 88
AflIII ACRYGT 1 cut(s) 103
AgsI TTSAA 6 cut(s) 200, 386, 483, 500, 662, 888
AjnI CCWGG 2 cut(s) 87, 813
AluBI AGCT 5 cut(s) 64, 96, 322, 578, 924
AluI AGCT 5 cut(s) 64, 96, 322, 578, 924
Alw21I GWGCWC 1 cut(s) 39
AlwI GGATC 2 cut(s) 79, 570
Ama87I CYCGRG 1 cut(s) 333
Aor13HI TCCGGA 1 cut(s) 892
Aor51HI AGCGCT 1 cut(s) 930
ApeKI GCWGC 3 cut(s) 119, 821, 931
ApoI RAATTY 2 cut(s) 461, 725
AspLEI GCGC 1 cut(s) 931
AspS9I GGNCC 1 cut(s) 79
AsuII TTCGAA 1 cut(s) 553
AsuNHI GCTAGC 1 cut(s) 92
AvaI CYCGRG 1 cut(s) 333
AvaII GGWCC 1 cut(s) 79
BbsI GAAGAC 1 cut(s) 863
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 3 cut(s) 106, 833, 918
BccI CCATC 2 cut(s) 247, 463
BciT130I CCWGG 2 cut(s) 89, 815
BclI TGATCA 1 cut(s) 615
BfaI CTAG 1 cut(s) 93
BfmI CTRYAG 2 cut(s) 593, 932
BfoI RGCGCY 1 cut(s) 932
BisI GCNGC 3 cut(s) 120, 822, 932
BlpI GCTNAGC 1 cut(s) 925
BlsI GCNGC 3 cut(s) 121, 823, 933
BmcAI AGTACT 1 cut(s) 718
Bme1390I CCNGG 2 cut(s) 89, 815
Bme18I GGWCC 1 cut(s) 79
BmeT110I CYCGRG 1 cut(s) 333
BmgT120I GGNCC 1 cut(s) 79
BmrFI CCNGG 2 cut(s) 89, 815
BmsI GCATC 3 cut(s) 355, 391, 823
BmtI GCTAGC 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 863
Bpu1102I GCTNAGC 1 cut(s) 925
Bpu14I TTCGAA 1 cut(s) 553
BsaWI WCCGGW 1 cut(s) 892
Bsc4I CCNNNNNNNGG 1 cut(s) 88
Bse1I ACTGG 1 cut(s) 172
Bse3DI GCAATG 1 cut(s) 589
BseAI TCCGGA 1 cut(s) 892
BseBI CCWGG 2 cut(s) 89, 815
BseGI GGATG 1 cut(s) 283
BseLI CCNNNNNNNGG 1 cut(s) 88
BseMI GCAATG 1 cut(s) 589
BseNI ACTGG 1 cut(s) 172
BseXI GCAGC 3 cut(s) 106, 833, 918
BsgI GTGCAG 2 cut(s) 105, 900
Bsh1236I CGCG 1 cut(s) 747
BsiHKAI GWGCWC 1 cut(s) 39
BsiHKCI CYCGRG 1 cut(s) 333
BsiSI CCGG 1 cut(s) 893
BslI CCNNNNNNNGG 1 cut(s) 88
BsmI GAATGC 2 cut(s) 121, 549
BsoBI CYCGRG 1 cut(s) 333
Bsp119I TTCGAA 1 cut(s) 553
Bsp1286I GDGCHC 1 cut(s) 39
Bsp13I TCCGGA 1 cut(s) 892
Bsp143I GATC 4 cut(s) 84, 540, 562, 615
Bsp1720I GCTNAGC 1 cut(s) 925
BspACI CCGC 2 cut(s) 745, 853
BspEI TCCGGA 1 cut(s) 892
BspFNI CGCG 1 cut(s) 747
BspMAI CTGCAG 1 cut(s) 936
BspOI GCTAGC 1 cut(s) 96
BspPI GGATC 2 cut(s) 79, 570
BspQI GCTCTTC 1 cut(s) 54
BspT104I TTCGAA 1 cut(s) 553
BsrDI GCAATG 1 cut(s) 589
BsrI ACTGG 1 cut(s) 172
BssMI GATC 4 cut(s) 84, 540, 562, 615
Bst2UI CCWGG 2 cut(s) 89, 815
Bst4CI ACNGT 4 cut(s) 293, 355, 622, 676
Bst6I CTCTTC 1 cut(s) 54
BstAPI GCANNNNNTGC 2 cut(s) 116, 827
BstBI TTCGAA 1 cut(s) 553
BstC8I GCNNGC 3 cut(s) 94, 366, 580
BstDEI CTNAG 1 cut(s) 925
BstF5I GGATG 1 cut(s) 283
BstFNI CGCG 1 cut(s) 747
BstH2I RGCGCY 1 cut(s) 932
BstHHI GCGC 1 cut(s) 931
BstKTI GATC 4 cut(s) 87, 543, 565, 618
BstMBI GATC 4 cut(s) 84, 540, 562, 615
BstMWI GCNNNNNNNGC 3 cut(s) 116, 401, 827
BstNI CCWGG 2 cut(s) 89, 815
BstNSI RCATGY 2 cut(s) 107, 368
BstSCI CCNGG 2 cut(s) 87, 813
BstSFI CTRYAG 2 cut(s) 593, 932
BstUI CGCG 1 cut(s) 747
BstV1I GCAGC 3 cut(s) 106, 833, 918
BstV2I GAAGAC 1 cut(s) 863
BstX2I RGATCY 1 cut(s) 562
BstXI CCANNNNNNTGG 1 cut(s) 578
BstYI RGATCY 1 cut(s) 562
BtsCI GGATG 1 cut(s) 283
BtsIMutI CAGTG 2 cut(s) 165, 618
Cac8I GCNNGC 3 cut(s) 94, 366, 580
CfoI GCGC 1 cut(s) 931
Cfr13I GGNCC 1 cut(s) 79
CseI GACGC 1 cut(s) 171
CsiI ACCWGGT 1 cut(s) 813
Csp6I GTAC 2 cut(s) 717, 881
CviAII CATG 3 cut(s) 104, 365, 391
CviQI GTAC 2 cut(s) 717, 881
DdeI CTNAG 1 cut(s) 925
DpnI GATC 4 cut(s) 86, 542, 564, 617
DpnII GATC 4 cut(s) 84, 540, 562, 615
DrdI GACNNNNNNGTC 2 cut(s) 26, 785
DseDI GACNNNNNNGTC 2 cut(s) 26, 785
Eam1104I CTCTTC 1 cut(s) 54
EarI CTCTTC 1 cut(s) 54
Eco47I GGWCC 1 cut(s) 79
Eco47III AGCGCT 1 cut(s) 930
Eco57I CTGAAG 3 cut(s) 78, 542, 687
Eco88I CYCGRG 1 cut(s) 333
EcoO109I RGGNCCY 1 cut(s) 79
EcoRI GAATTC 1 cut(s) 461
EcoRII CCWGG 2 cut(s) 87, 813
FaeI CATG 3 cut(s) 107, 368, 394
FalI AAGNNNNNCTT 2 cut(s) 122, 154
FatI CATG 3 cut(s) 103, 364, 390
FauNDI CATATG 1 cut(s) 862
FbaI TGATCA 1 cut(s) 615
FblI GTMKAC 2 cut(s) 30, 795
Fnu4HI GCNGC 3 cut(s) 120, 822, 932
FokI GGATG 1 cut(s) 290
Fsp4HI GCNGC 3 cut(s) 120, 822, 932
FspBI CTAG 1 cut(s) 93
GlaI GCGC 1 cut(s) 930
GluI GCNGC 3 cut(s) 120, 822, 932
HaeII RGCGCY 1 cut(s) 932
HapII CCGG 1 cut(s) 893
HgaI GACGC 1 cut(s) 171
HhaI GCGC 1 cut(s) 931
Hin1II CATG 3 cut(s) 107, 368, 394
Hin6I GCGC 1 cut(s) 929
HinP1I GCGC 1 cut(s) 929
HincII GTYRAC 3 cut(s) 31, 181, 742
HindII GTYRAC 3 cut(s) 31, 181, 742
HindIII AAGCTT 1 cut(s) 922
HinfI GANTC 6 cut(s) 53, 206, 331, 440, 479, 732
HpaII CCGG 1 cut(s) 893
Hpy166II GTNNAC 4 cut(s) 31, 181, 742, 796
Hpy188I TCNGA 8 cut(s) 58, 309, 351, 460, 489, 508, 706, 731
Hpy188III TCNNGA 4 cut(s) 466, 659, 776, 893
Hpy8I GTNNAC 4 cut(s) 31, 181, 742, 796
Hpy99I CGWCG 1 cut(s) 35
HpyAV CCTTC 4 cut(s) 70, 370, 517, 552
HpyCH4III ACNGT 4 cut(s) 293, 355, 622, 676
HpyCH4IV ACGT 2 cut(s) 202, 503
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 401, 827
HpyF3I CTNAG 1 cut(s) 925
HpySE526I ACGT 2 cut(s) 202, 503
Hsp92II CATG 3 cut(s) 107, 368, 394
HspAI GCGC 1 cut(s) 929
Kpn2I TCCGGA 1 cut(s) 892
Ksp22I TGATCA 1 cut(s) 615
Kzo9I GATC 4 cut(s) 84, 540, 562, 615
LguI GCTCTTC 1 cut(s) 54
Lsp1109I GCAGC 3 cut(s) 106, 833, 918
LweI GCATC 3 cut(s) 355, 391, 823
MabI ACCWGGT 1 cut(s) 813
MaeI CTAG 1 cut(s) 93
MaeII ACGT 2 cut(s) 202, 503
MaeIII GTNAC 5 cut(s) 203, 287, 586, 676, 752
MalI GATC 4 cut(s) 86, 542, 564, 617
MboI GATC 4 cut(s) 84, 540, 562, 615
MboII GAAGA 7 cut(s) 22, 71, 567, 614, 693, 868, 916
MflI RGATCY 1 cut(s) 562
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 7 cut(s) 232, 461, 483, 495, 627, 725, 844
MlyI GAGTC 3 cut(s) 200, 325, 434
MnlI CCTC 7 cut(s) 240, 268, 520, 592, 666, 677, 861
MroI TCCGGA 1 cut(s) 892
MseI TTAA 1 cut(s) 624
MspA1I CMGCKG 1 cut(s) 853
MspI CCGG 1 cut(s) 893
MspR9I CCNGG 2 cut(s) 89, 815
Mva1269I GAATGC 2 cut(s) 121, 549
MvaI CCWGG 2 cut(s) 89, 815
MvnI CGCG 1 cut(s) 747
MwoI GCNNNNNNNGC 3 cut(s) 116, 401, 827
NdeI CATATG 1 cut(s) 862
NdeII GATC 4 cut(s) 84, 540, 562, 615
NheI GCTAGC 1 cut(s) 92
NlaIII CATG 3 cut(s) 107, 368, 394
NmuCI GTSAC 3 cut(s) 203, 676, 752
NspI RCATGY 2 cut(s) 107, 368
NspV TTCGAA 1 cut(s) 553
PaeI GCATGC 1 cut(s) 368
PciI ACATGT 1 cut(s) 103
PciSI GCTCTTC 1 cut(s) 54
PcsI WCGNNNNNNNCGW 1 cut(s) 208
PctI GAATGC 2 cut(s) 121, 549
PfeI GAWTC 3 cut(s) 53, 479, 732
PkrI GCNGC 3 cut(s) 121, 823, 933
PleI GAGTC 3 cut(s) 200, 325, 434
PpsI GAGTC 3 cut(s) 200, 325, 434
PpuMI RGGWCCY 1 cut(s) 79
PscI ACATGT 1 cut(s) 103
Psp5II RGGWCCY 1 cut(s) 79
Psp6I CCWGG 2 cut(s) 87, 813
PspGI CCWGG 2 cut(s) 87, 813
PspPI GGNCC 1 cut(s) 79
PspPPI RGGWCCY 1 cut(s) 79
PstI CTGCAG 1 cut(s) 936
PsuI RGATCY 1 cut(s) 562
RsaI GTAC 2 cut(s) 718, 882
RsaNI GTAC 2 cut(s) 717, 881
SalI GTCGAC 1 cut(s) 29
SapI GCTCTTC 1 cut(s) 54
SaqAI TTAA 1 cut(s) 624
SatI GCNGC 3 cut(s) 120, 822, 932
Sau3AI GATC 4 cut(s) 84, 540, 562, 615
Sau96I GGNCC 1 cut(s) 79
ScaI AGTACT 1 cut(s) 718
SchI GAGTC 3 cut(s) 200, 325, 434
ScrFI CCNGG 2 cut(s) 89, 815
SduI GDGCHC 1 cut(s) 39
SetI ASST 9 cut(s) 66, 84, 98, 205, 324, 506, 580, 816, 926
SexAI ACCWGGT 1 cut(s) 813
SfaNI GCATC 3 cut(s) 355, 391, 823
SfcI CTRYAG 2 cut(s) 593, 932
SfuI TTCGAA 1 cut(s) 553
SinI GGWCC 1 cut(s) 79
SphI GCATGC 1 cut(s) 368
Sse9I AATT 7 cut(s) 232, 461, 483, 495, 627, 725, 844
SsiI CCGC 2 cut(s) 745, 853
SspMI CTAG 1 cut(s) 93
StyD4I CCNGG 2 cut(s) 87, 813
TaaI ACNGT 4 cut(s) 293, 355, 622, 676
TaiI ACGT 2 cut(s) 205, 506
TaqI TCGA 3 cut(s) 30, 553, 809
TasI AATT 7 cut(s) 232, 461, 483, 495, 627, 725, 844
TatI WGTACW 2 cut(s) 716, 880
TfiI GAWTC 3 cut(s) 53, 479, 732
Tru1I TTAA 1 cut(s) 624
Tru9I TTAA 1 cut(s) 624
TscAI CASTG 2 cut(s) 172, 625
TseFI GTSAC 3 cut(s) 203, 676, 752
TseI GCWGC 3 cut(s) 119, 821, 931
Tsp45I GTSAC 3 cut(s) 203, 676, 752
TspDTI ATGAA 1 cut(s) 315
TspRI CASTG 2 cut(s) 172, 625
VpaK11BI GGWCC 1 cut(s) 79
XapI RAATTY 2 cut(s) 461, 725
XceI RCATGY 2 cut(s) 107, 368
XcmI CCANNNNNNNNNTGG 1 cut(s) 462
XmiI GTMKAC 2 cut(s) 30, 795
XspI CTAG 1 cut(s) 93
ZrmI AGTACT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.