AT5G46850

PIG-X / PBN1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007198
Physical Location & Seq
Forward (+)
19010608 .. 19012038
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46850.3

Sequence Viewer

Length: 891 bp
ATGGGCTTCTTTCGTGCTGCAACTCATCCAGATGATCTCCGGCGACGAGTACCTTCGCCGGCAATAATGGTTGGAACAAATTATAGAGATTCTCATGAATTTTTCGATTCCGATACAATCTGTATCTCTGATCAATACATTATGGAGTCTTATTTCACTACATACACTTCCTTAGACGACTCAGAGTTTCGAAACTTCTTGCTTCTTCAACAATGTTCTGACAAAGTTCCTACTCAAGATTCAAACATTGTTCCTGCAACTTCATTTATACAACATAGAAACATTACTGGTGAAGGTTCACATCGTAATCTCATCACATCCATCAAGCTTCTTCTTCATCACTCAGAGTCTCAGTTTCGTGAGCTCGTGATCGTTGAACGGTTGCCTTTGGGTGTTTTTGCTGATCCTTTTGAGCTTCAGAGTCTTCAACAACGCAGAGCTTTCAGTGACGTATCTGTGTTTGGTGACACAAATCTTGAGCTGCCTTCGTTTAGATCGAACAGATCCGTCGTTGAGATTCATGTTGAAATCAGCTCTAGAAATTATGAAAATGGTGAAATAAGCTTTAAGCTTCCTTTACATGCTCGGTATCAGCCTCTGGACGACAGTGGATACTCTAGAGTGGAGTTTGGCGAGCCAGACTTGTTCTTGTGCTCGAGCCATGTACAAAACCAGCGACGTGAACGGAGGAGATGTTTGGTTTTGTCGATTGGCAGATCGAAGACTGAAACCAGGTCTGTTTTCTGGGATATCCCTGCCGGAATCAGGGGTCATACAGAGTATGTTTCCGCCTTGACATTCGCCGCTGCGGTTTTTTCTGCATTTTCAATACTCGTGGCATCGGTTTTGAGCTCAAAGGGTGAATCTTGTAAAAACTTGAAGCAGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PIG-X

Protein Analysis

296

Amino Acids

33.7

Weight (kDa)

6.21

Isoelectric Point (pI)

56.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIG-X PF08320 102 - 283 7.4e-22 PIG-X / PBN1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 789, 804, 809
AclWI GGATC 2 cut(s) 398, 498
AcsI RAATTY 1 cut(s) 98
AcuI CTGAAG 1 cut(s) 401
AfaI GTAC 2 cut(s) 51, 666
AfiI CCNNNNNNNGG 1 cut(s) 765
AgsI TTSAA 7 cut(s) 209, 243, 377, 428, 527, 828, 880
AjiI CACGTC 1 cut(s) 680
AjnI CCWGG 1 cut(s) 731
AluBI AGCT 9 cut(s) 328, 364, 415, 440, 481, 534, 564, 571, 852
AluI AGCT 9 cut(s) 328, 364, 415, 440, 481, 534, 564, 571, 852
Alw21I GWGCWC 3 cut(s) 366, 656, 854
Alw26I GTCTC 1 cut(s) 354
AlwI GGATC 2 cut(s) 398, 498
AlwNI CAGNNNCTG 1 cut(s) 598
Ama87I CYCGRG 1 cut(s) 655
ApeKI GCWGC 3 cut(s) 17, 481, 806
ApoI RAATTY 1 cut(s) 98
AsuHPI GGTGA 4 cut(s) 302, 476, 566, 872
AsuII TTCGAA 1 cut(s) 190
AvaI CYCGRG 1 cut(s) 655
BanII GRGCYC 2 cut(s) 366, 854
BauI CACGAG 2 cut(s) 365, 833
BbsI GAAGAC 2 cut(s) 416, 728
Bbv12I GWGCWC 3 cut(s) 366, 656, 854
BbvI GCAGC 3 cut(s) 4, 468, 793
BccI CCATC 1 cut(s) 329
BciT130I CCWGG 1 cut(s) 733
BciVI GTATCC 1 cut(s) 605
BclI TGATCA 1 cut(s) 130
BcoDI GTCTC 1 cut(s) 354
BfaI CTAG 2 cut(s) 537, 618
BfuI GTATCC 1 cut(s) 605
BisI GCNGC 4 cut(s) 18, 482, 804, 807
BlsI GCNGC 4 cut(s) 19, 483, 805, 808
Bme1390I CCNGG 1 cut(s) 733
BmeT110I CYCGRG 1 cut(s) 655
BmgBI CACGTC 1 cut(s) 680
BmrFI CCNGG 1 cut(s) 733
BmsI GCATC 1 cut(s) 848
BpiI GAAGAC 2 cut(s) 416, 728
Bpu14I TTCGAA 1 cut(s) 190
BpuEI CTTGAG 2 cut(s) 219, 497
BsaXI ACNNNNNCTCC 2 cut(s) 679, 709
Bsc4I CCNNNNNNNGG 1 cut(s) 765
Bse118I RCCGGY 1 cut(s) 58
Bse1I ACTGG 1 cut(s) 292
BseBI CCWGG 1 cut(s) 733
BseGI GGATG 2 cut(s) 25, 317
BseLI CCNNNNNNNGG 1 cut(s) 765
BseMII CTCAG 3 cut(s) 195, 357, 365
BseNI ACTGG 1 cut(s) 292
BseRI GAGGAG 1 cut(s) 703
BseXI GCAGC 3 cut(s) 4, 468, 793
BsiHKAI GWGCWC 3 cut(s) 366, 656, 854
BsiHKCI CYCGRG 1 cut(s) 655
BsiSI CCGG 3 cut(s) 40, 59, 759
BslI CCNNNNNNNGG 1 cut(s) 765
BsmAI GTCTC 1 cut(s) 354
BsoBI CYCGRG 1 cut(s) 655
Bsp119I TTCGAA 1 cut(s) 190
Bsp1286I GDGCHC 3 cut(s) 366, 656, 854
Bsp1407I TGTACA 1 cut(s) 664
Bsp143I GATC 7 cut(s) 34, 130, 369, 403, 494, 503, 716
BspACI CCGC 3 cut(s) 789, 804, 809
BspCNI CTCAG 3 cut(s) 194, 356, 364
BspHI TCATGA 2 cut(s) 94, 887
BspPI GGATC 2 cut(s) 398, 498
BspT104I TTCGAA 1 cut(s) 190
BsrFI RCCGGY 1 cut(s) 58
BsrGI TGTACA 1 cut(s) 664
BsrI ACTGG 1 cut(s) 292
BssAI RCCGGY 1 cut(s) 58
BssMI GATC 7 cut(s) 34, 130, 369, 403, 494, 503, 716
BssSI CACGAG 2 cut(s) 365, 833
Bst2BI CACGAG 2 cut(s) 365, 833
Bst2UI CCWGG 1 cut(s) 733
Bst4CI ACNGT 2 cut(s) 381, 608
BstAUI TGTACA 1 cut(s) 664
BstBI TTCGAA 1 cut(s) 190
BstC8I GCNNGC 2 cut(s) 60, 635
BstDEI CTNAG 4 cut(s) 172, 181, 343, 351
BstF5I GGATG 2 cut(s) 25, 317
BstKTI GATC 7 cut(s) 37, 133, 372, 406, 497, 506, 719
BstMAI GTCTC 1 cut(s) 354
BstMBI GATC 7 cut(s) 34, 130, 369, 403, 494, 503, 716
BstNI CCWGG 1 cut(s) 733
BstNSI RCATGY 1 cut(s) 584
BstSCI CCNGG 1 cut(s) 731
BstV1I GCAGC 3 cut(s) 4, 468, 793
BstV2I GAAGAC 2 cut(s) 416, 728
BstX2I RGATCY 1 cut(s) 503
BstYI RGATCY 1 cut(s) 503
BsuI GTATCC 1 cut(s) 605
BtrI CACGTC 1 cut(s) 680
BtsCI GGATG 2 cut(s) 25, 317
BtsIMutI CAGTG 2 cut(s) 451, 613
Cac8I GCNNGC 2 cut(s) 60, 635
CaiI CAGNNNCTG 1 cut(s) 598
CciI TCATGA 2 cut(s) 94, 887
Cfr10I RCCGGY 1 cut(s) 58
CsiI ACCWGGT 1 cut(s) 731
Csp6I GTAC 2 cut(s) 50, 665
CspCI CAANNNNNGTGG 2 cut(s) 816, 851
CviAII CATG 5 cut(s) 95, 521, 581, 662, 888
CviQI GTAC 2 cut(s) 50, 665
DdeI CTNAG 4 cut(s) 172, 181, 343, 351
DpnI GATC 7 cut(s) 36, 132, 371, 405, 496, 505, 718
DpnII GATC 7 cut(s) 34, 130, 369, 403, 494, 503, 716
EciI GGCGGA 1 cut(s) 778
Ecl136II GAGCTC 2 cut(s) 364, 852
Eco24I GRGCYC 2 cut(s) 366, 854
Eco32I GATATC 1 cut(s) 751
Eco53kI GAGCTC 2 cut(s) 364, 852
Eco57I CTGAAG 1 cut(s) 401
Eco88I CYCGRG 1 cut(s) 655
EcoICRI GAGCTC 2 cut(s) 364, 852
EcoRII CCWGG 1 cut(s) 731
EcoRV GATATC 1 cut(s) 751
EcoT38I GRGCYC 2 cut(s) 366, 854
FaeI CATG 5 cut(s) 98, 524, 584, 665, 891
FatI CATG 5 cut(s) 94, 520, 580, 661, 887
FbaI TGATCA 1 cut(s) 130
Fnu4HI GCNGC 4 cut(s) 18, 482, 804, 807
FokI GGATG 2 cut(s) 12, 304
FriOI GRGCYC 2 cut(s) 366, 854
Fsp4HI GCNGC 4 cut(s) 18, 482, 804, 807
FspBI CTAG 2 cut(s) 537, 618
GluI GCNGC 4 cut(s) 18, 482, 804, 807
HapII CCGG 3 cut(s) 40, 59, 759
Hin1II CATG 5 cut(s) 98, 524, 584, 665, 891
HindIII AAGCTT 3 cut(s) 326, 562, 569
HpaII CCGG 3 cut(s) 40, 59, 759
HphI GGTGA 4 cut(s) 302, 476, 566, 872
Hpy166II GTNNAC 2 cut(s) 299, 683
Hpy188I TCNGA 6 cut(s) 112, 130, 184, 220, 346, 420
Hpy8I GTNNAC 2 cut(s) 299, 683
Hpy99I CGWCG 3 cut(s) 48, 512, 681
HpyAV CCTTC 3 cut(s) 63, 287, 495
HpyCH4III ACNGT 2 cut(s) 381, 608
HpyCH4IV ACGT 2 cut(s) 450, 679
HpyCH4V TGCA 3 cut(s) 20, 257, 821
HpyF3I CTNAG 4 cut(s) 172, 181, 343, 351
HpySE526I ACGT 2 cut(s) 450, 679
Hsp92II CATG 5 cut(s) 98, 524, 584, 665, 891
KroI GCCGGC 1 cut(s) 58
KroNI GCCGGC 1 cut(s) 60
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 7 cut(s) 34, 130, 369, 403, 494, 503, 716
Lsp1109I GCAGC 3 cut(s) 4, 468, 793
LweI GCATC 1 cut(s) 848
MabI ACCWGGT 1 cut(s) 731
MaeI CTAG 2 cut(s) 537, 618
MaeII ACGT 2 cut(s) 450, 679
MaeIII GTNAC 2 cut(s) 446, 464
MalI GATC 7 cut(s) 36, 132, 371, 405, 496, 505, 718
MboI GATC 7 cut(s) 34, 130, 369, 403, 494, 503, 716
MboII GAAGA 5 cut(s) 197, 323, 326, 416, 733
MflI RGATCY 1 cut(s) 503
MhlI GDGCHC 3 cut(s) 366, 656, 854
MluCI AATT 3 cut(s) 79, 98, 541
MlyI GAGTC 4 cut(s) 155, 173, 356, 430
MmeI TCCRAC 1 cut(s) 52
MnlI CCTC 2 cut(s) 606, 681
MroNI GCCGGC 1 cut(s) 58
MseI TTAA 1 cut(s) 567
MslI CAYNNNNRTG 1 cut(s) 30
MspA1I CMGCKG 1 cut(s) 806
MspI CCGG 3 cut(s) 40, 59, 759
MspR9I CCNGG 1 cut(s) 733
MvaI CCWGG 1 cut(s) 733
NaeI GCCGGC 1 cut(s) 60
NdeII GATC 7 cut(s) 34, 130, 369, 403, 494, 503, 716
NgoMIV GCCGGC 1 cut(s) 58
NlaIII CATG 5 cut(s) 98, 524, 584, 665, 891
NmuCI GTSAC 2 cut(s) 446, 464
NspI RCATGY 1 cut(s) 584
NspV TTCGAA 1 cut(s) 190
PaeR7I CTCGAG 1 cut(s) 655
PagI TCATGA 2 cut(s) 94, 887
PcsI WCGNNNNNNNCGW 1 cut(s) 494
PdiI GCCGGC 1 cut(s) 60
PfeI GAWTC 6 cut(s) 89, 107, 239, 517, 762, 863
PkrI GCNGC 4 cut(s) 19, 483, 805, 808
PleI GAGTC 4 cut(s) 154, 173, 355, 429
PpsI GAGTC 4 cut(s) 154, 173, 355, 429
Psp124BI GAGCTC 2 cut(s) 366, 854
Psp6I CCWGG 1 cut(s) 731
PspGI CCWGG 1 cut(s) 731
PspXI VCTCGAGB 1 cut(s) 655
PstNI CAGNNNCTG 1 cut(s) 598
PsuI RGATCY 1 cut(s) 503
RsaI GTAC 2 cut(s) 51, 666
RsaNI GTAC 2 cut(s) 50, 665
RseI CAYNNNNRTG 1 cut(s) 30
SacI GAGCTC 2 cut(s) 366, 854
SaqAI TTAA 1 cut(s) 567
SatI GCNGC 4 cut(s) 18, 482, 804, 807
Sau3AI GATC 7 cut(s) 34, 130, 369, 403, 494, 503, 716
SchI GAGTC 4 cut(s) 155, 173, 356, 430
ScrFI CCNGG 1 cut(s) 733
SduI GDGCHC 3 cut(s) 366, 656, 854
SexAI ACCWGGT 1 cut(s) 731
SfaNI GCATC 1 cut(s) 848
Sfr274I CTCGAG 1 cut(s) 655
SfuI TTCGAA 1 cut(s) 190
SlaI CTCGAG 1 cut(s) 655
SmiMI CAYNNNNRTG 1 cut(s) 30
SmlI CTYRAG 3 cut(s) 234, 476, 655
SmoI CTYRAG 3 cut(s) 234, 476, 655
Sse9I AATT 3 cut(s) 79, 98, 541
SsiI CCGC 3 cut(s) 789, 804, 809
SspMI CTAG 2 cut(s) 537, 618
SstI GAGCTC 2 cut(s) 366, 854
StyD4I CCNGG 1 cut(s) 731
TaaI ACNGT 2 cut(s) 381, 608
TaiI ACGT 2 cut(s) 453, 682
TaqI TCGA 6 cut(s) 105, 190, 497, 656, 707, 719
TasI AATT 3 cut(s) 79, 98, 541
TatI WGTACW 1 cut(s) 664
TauI GCSGC 1 cut(s) 806
TfiI GAWTC 6 cut(s) 89, 107, 239, 517, 762, 863
Tru1I TTAA 1 cut(s) 567
Tru9I TTAA 1 cut(s) 567
TscAI CASTG 2 cut(s) 451, 613
TseFI GTSAC 2 cut(s) 446, 464
TseI GCWGC 3 cut(s) 17, 481, 806
Tsp45I GTSAC 2 cut(s) 446, 464
TspDTI ATGAA 5 cut(s) 111, 252, 326, 509, 561
TspGWI ACGGA 2 cut(s) 496, 700
TspRI CASTG 2 cut(s) 451, 613
XapI RAATTY 1 cut(s) 98
XbaI TCTAGA 2 cut(s) 536, 617
XceI RCATGY 1 cut(s) 584
XhoI CTCGAG 1 cut(s) 655
XspI CTAG 2 cut(s) 537, 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.