MD06G1038100.v1.1

Phosphatidylinositol-glycan biosynthesis class X

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007198
Physical Location & Seq
Forward (+)
4863924 .. 4866710
2787 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1038100.v1.1.491

Sequence Viewer

Length: 999 bp
ATGAAAGTAGAAACCATGAAGGCAAGAACATCCCAGCCATCAGCCGCCGTTGCCTTCTCCAATTCCTTCCTTCTGGGATTTGCCTTGGTATTCTTCTCCGCCATTGGTTTCTGCACACATTCCTCTGCACATTATTCAACTCATCAGGCGGGCAGTGATTCAAACTATGATGGTGGTGGCTCAAATATCAGTGCCTTTTCTAGTTTCAACAAGTATATTATGCAGTCTTATTACGAAAAATATGAAAACTTGTTCGATTCAGATTTCCAAGATTTTGTAGCACAAGAGCTTCCTTTTGGTTTGTGTGAGGTGCTGCCACATGATCTGAGTCTTGTTGTTAGACCCCCAATTTTGCGCCGCAATTTGATAGGCAAAGGTTCTCATCGTCATCTCACCTCATCCATTAGAGTAAACATCAAACCAGAGCCCATATTTGAGCTACCAACTCACCATTGTGAGCTCATAATCATTGAGAGACTGCCATCTGGAGTATTTGCAGATCCATTTGAGCTTCAACATCTCCTTCAGCGTGGTGCATATAGCGCTATAGCTGTGTTTGGAGATGCAAATTTGGAACTGCCTTCATTTCTTTCTAATCGTACCTCTGTTGAGGTTCACATGGATATTGGTCATAATTTTTTATTGAGGCATGACAAAGAGTCTAACATCAACATAGAGCTTCCATTGCATGCACGATATCCACCTCTAGATGAAAGCGGCTATTGGGAAGTCAAATTTGGTATGCCGGATTTGTTCATGCGTTGTAGCATTGAGGGAACTTCACAAAATCAAAGCTGTTTGTATAAGATGCCAGAGATCAATGGTTCTGAATTAGGATACAGTAAACTTGTCTGGAAAATCCCTTCTGGGATGAAGGCGCATGCTGGAATCGTATCCACTCTCACGTTCGTTGCAGCCTTAATGTCGGCTTTTGCAATTGCTGCCACATCCATTTTTCATTCAGGCATTGCCTCCGGCAAACATTTGAAAGAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PIG-X

Protein Analysis

333

Amino Acids

36.84

Weight (kDa)

6.23

Isoelectric Point (pI)

52.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIG-X PF08320 125 - 314 4e-23 PIG-X / PBN1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 45, 99, 149, 358, 717
AclWI GGATC 1 cut(s) 494
AcsI RAATTY 2 cut(s) 568, 734
AcuI CTGAAG 1 cut(s) 509
AfaI GTAC 1 cut(s) 601
AfeI AGCGCT 1 cut(s) 544
AgsI TTSAA 5 cut(s) 138, 162, 208, 515, 988
AhdI GACNNNNNGTC 1 cut(s) 658
AjuI GAANNNNNNNTTGG 2 cut(s) 720, 752
AleI CACNNNNGTG 1 cut(s) 453
AluBI AGCT 7 cut(s) 289, 439, 460, 511, 551, 679, 795
AluI AGCT 7 cut(s) 289, 439, 460, 511, 551, 679, 795
Alw21I GWGCWC 1 cut(s) 462
Alw26I GTCTC 1 cut(s) 469
AlwI GGATC 1 cut(s) 494
Aor51HI AGCGCT 1 cut(s) 544
ApeKI GCWGC 3 cut(s) 313, 914, 941
ApoI RAATTY 2 cut(s) 568, 734
AspLEI GCGC 3 cut(s) 357, 545, 880
AsuHPI GGTGA 2 cut(s) 385, 440
BanII GRGCYC 2 cut(s) 429, 462
Bbv12I GWGCWC 1 cut(s) 462
BbvI GCAGC 3 cut(s) 300, 926, 928
BccI CCATC 3 cut(s) 46, 164, 490
BceAI ACGGC 1 cut(s) 32
BciVI GTATCC 2 cut(s) 830, 904
BcoDI GTCTC 1 cut(s) 469
BfaI CTAG 2 cut(s) 201, 707
BfmI CTRYAG 1 cut(s) 546
BfoI RGCGCY 1 cut(s) 546
BfuI GTATCC 2 cut(s) 830, 904
BisI GCNGC 6 cut(s) 45, 314, 358, 718, 915, 942
BlsI GCNGC 6 cut(s) 46, 315, 359, 719, 916, 943
BmeRI GACNNNNNGTC 1 cut(s) 658
BmsI GCATC 2 cut(s) 553, 798
BpmI CTGGAG 1 cut(s) 507
BsaJI CCNNGG 1 cut(s) 84
Bse3DI GCAATG 2 cut(s) 683, 966
BseDI CCNNGG 1 cut(s) 84
BseGI GGATG 4 cut(s) 29, 398, 876, 947
BseMI GCAATG 2 cut(s) 683, 966
BseMII CTCAG 1 cut(s) 317
BseXI GCAGC 3 cut(s) 300, 926, 928
BseYI CCCAGC 1 cut(s) 33
BsgI GTGCAG 2 cut(s) 97, 111
BsiHKAI GWGCWC 1 cut(s) 462
BsiSI CCGG 2 cut(s) 746, 975
BsmAI GTCTC 1 cut(s) 469
Bsp1286I GDGCHC 2 cut(s) 429, 462
Bsp143I GATC 3 cut(s) 322, 499, 816
BspACI CCGC 5 cut(s) 45, 99, 149, 358, 717
BspCNI CTCAG 1 cut(s) 318
BspPI GGATC 1 cut(s) 494
BsrDI GCAATG 2 cut(s) 683, 966
BssECI CCNNGG 1 cut(s) 84
BssMI GATC 3 cut(s) 322, 499, 816
BssT1I CCWWGG 1 cut(s) 84
Bst4CI ACNGT 1 cut(s) 842
BstAPI GCANNNNNTGC 1 cut(s) 941
BstC8I GCNNGC 3 cut(s) 151, 690, 882
BstDEI CTNAG 1 cut(s) 326
BstF5I GGATG 4 cut(s) 29, 398, 876, 947
BstH2I RGCGCY 1 cut(s) 546
BstHHI GCGC 3 cut(s) 357, 545, 880
BstKTI GATC 3 cut(s) 325, 502, 819
BstMAI GTCTC 1 cut(s) 469
BstMBI GATC 3 cut(s) 322, 499, 816
BstMWI GCNNNNNNNGC 4 cut(s) 50, 542, 685, 941
BstNSI RCATGY 2 cut(s) 692, 884
BstSFI CTRYAG 1 cut(s) 546
BstV1I GCAGC 3 cut(s) 300, 926, 928
BstX2I RGATCY 1 cut(s) 499
BstYI RGATCY 1 cut(s) 499
BsuI GTATCC 2 cut(s) 830, 904
BtsCI GGATG 4 cut(s) 29, 398, 876, 947
BtsI GCAGTG 1 cut(s) 160
BtsIMutI CAGTG 2 cut(s) 160, 196
Cac8I GCNNGC 3 cut(s) 151, 690, 882
CfoI GCGC 3 cut(s) 357, 545, 880
Csp6I GTAC 1 cut(s) 600
CviAII CATG 7 cut(s) 16, 320, 619, 650, 689, 757, 881
CviQI GTAC 1 cut(s) 600
DdeI CTNAG 1 cut(s) 326
DpnI GATC 3 cut(s) 324, 501, 818
DpnII GATC 3 cut(s) 322, 499, 816
DriI GACNNNNNGTC 1 cut(s) 658
Eam1105I GACNNNNNGTC 1 cut(s) 658
EciI GGCGGA 1 cut(s) 88
Ecl136II GAGCTC 1 cut(s) 460
Eco130I CCWWGG 1 cut(s) 84
Eco24I GRGCYC 2 cut(s) 429, 462
Eco32I GATATC 1 cut(s) 698
Eco47III AGCGCT 1 cut(s) 544
Eco53kI GAGCTC 1 cut(s) 460
Eco57I CTGAAG 1 cut(s) 509
EcoICRI GAGCTC 1 cut(s) 460
EcoRV GATATC 1 cut(s) 698
EcoT14I CCWWGG 1 cut(s) 84
EcoT38I GRGCYC 2 cut(s) 429, 462
ErhI CCWWGG 1 cut(s) 84
FaeI CATG 7 cut(s) 19, 323, 622, 653, 692, 760, 884
FatI CATG 7 cut(s) 15, 319, 618, 649, 688, 756, 880
FauI CCCGC 1 cut(s) 142
Fnu4HI GCNGC 6 cut(s) 45, 314, 358, 718, 915, 942
FokI GGATG 4 cut(s) 16, 385, 883, 934
FriOI GRGCYC 2 cut(s) 429, 462
Fsp4HI GCNGC 6 cut(s) 45, 314, 358, 718, 915, 942
FspBI CTAG 2 cut(s) 201, 707
GlaI GCGC 3 cut(s) 356, 544, 879
GluI GCNGC 6 cut(s) 45, 314, 358, 718, 915, 942
GsaI CCCAGC 1 cut(s) 37
GsuI CTGGAG 1 cut(s) 507
HaeII RGCGCY 1 cut(s) 546
HapII CCGG 2 cut(s) 746, 975
HhaI GCGC 3 cut(s) 357, 545, 880
Hin1II CATG 7 cut(s) 19, 323, 622, 653, 692, 760, 884
Hin6I GCGC 3 cut(s) 355, 543, 878
HinP1I GCGC 3 cut(s) 355, 543, 878
HinfI GANTC 6 cut(s) 158, 257, 328, 659, 888, 992
HpaII CCGG 2 cut(s) 746, 975
HphI GGTGA 2 cut(s) 385, 440
Hpy166II GTNNAC 3 cut(s) 412, 616, 845
Hpy188I TCNGA 3 cut(s) 262, 327, 829
Hpy188III TCNNGA 3 cut(s) 486, 707, 853
Hpy8I GTNNAC 3 cut(s) 412, 616, 845
HpyAV CCTTC 8 cut(s) 13, 64, 76, 80, 533, 591, 868, 873
HpyCH4III ACNGT 1 cut(s) 842
HpyCH4IV ACGT 1 cut(s) 905
HpyF10VI GCNNNNNNNGC 4 cut(s) 50, 542, 685, 941
HpyF3I CTNAG 1 cut(s) 326
HpySE526I ACGT 1 cut(s) 905
Hsp92II CATG 7 cut(s) 19, 323, 622, 653, 692, 760, 884
HspAI GCGC 3 cut(s) 355, 543, 878
Kzo9I GATC 3 cut(s) 322, 499, 816
Lsp1109I GCAGC 3 cut(s) 300, 926, 928
LweI GCATC 2 cut(s) 553, 798
MaeI CTAG 2 cut(s) 201, 707
MaeII ACGT 1 cut(s) 905
MalI GATC 3 cut(s) 324, 501, 818
MboI GATC 3 cut(s) 322, 499, 816
MboII GAAGA 1 cut(s) 85
MfeI CAATTG 1 cut(s) 936
MflI RGATCY 1 cut(s) 499
MhlI GDGCHC 2 cut(s) 429, 462
MluCI AATT 8 cut(s) 61, 348, 361, 568, 634, 734, 830, 936
MlyI GAGTC 2 cut(s) 337, 668
MnlI CCTC 9 cut(s) 133, 301, 406, 604, 613, 639, 714, 766, 982
MseI TTAA 2 cut(s) 920, 997
MslI CAYNNNNRTG 1 cut(s) 453
MspI CCGG 2 cut(s) 746, 975
MunI CAATTG 1 cut(s) 936
MwoI GCNNNNNNNGC 4 cut(s) 50, 542, 685, 941
NdeII GATC 3 cut(s) 322, 499, 816
NlaIII CATG 7 cut(s) 19, 323, 622, 653, 692, 760, 884
NspI RCATGY 2 cut(s) 692, 884
OliI CACNNNNGTG 1 cut(s) 453
PaeI GCATGC 2 cut(s) 692, 884
PfeI GAWTC 4 cut(s) 158, 257, 888, 992
PkrI GCNGC 6 cut(s) 46, 315, 359, 719, 916, 943
PleI GAGTC 2 cut(s) 336, 667
PpsI GAGTC 2 cut(s) 336, 667
Psp124BI GAGCTC 1 cut(s) 462
PspFI CCCAGC 1 cut(s) 33
PsuI RGATCY 1 cut(s) 499
RsaI GTAC 1 cut(s) 601
RsaNI GTAC 1 cut(s) 600
RseI CAYNNNNRTG 1 cut(s) 453
SacI GAGCTC 1 cut(s) 462
SaqAI TTAA 2 cut(s) 920, 997
SatI GCNGC 6 cut(s) 45, 314, 358, 718, 915, 942
Sau3AI GATC 3 cut(s) 322, 499, 816
SchI GAGTC 2 cut(s) 337, 668
SduI GDGCHC 2 cut(s) 429, 462
SfaNI GCATC 2 cut(s) 553, 798
SfcI CTRYAG 1 cut(s) 546
SmiMI CAYNNNNRTG 1 cut(s) 453
SphI GCATGC 2 cut(s) 692, 884
Sse9I AATT 8 cut(s) 61, 348, 361, 568, 634, 734, 830, 936
SsiI CCGC 5 cut(s) 45, 99, 149, 358, 717
SspMI CTAG 2 cut(s) 201, 707
SstI GAGCTC 1 cut(s) 462
StyI CCWWGG 1 cut(s) 84
TaaI ACNGT 1 cut(s) 842
TaiI ACGT 1 cut(s) 908
TaqI TCGA 1 cut(s) 255
TasI AATT 8 cut(s) 61, 348, 361, 568, 634, 734, 830, 936
TauI GCSGC 3 cut(s) 47, 360, 720
TfiI GAWTC 4 cut(s) 158, 257, 888, 992
Tru1I TTAA 2 cut(s) 920, 997
Tru9I TTAA 2 cut(s) 920, 997
TscAI CASTG 2 cut(s) 160, 196
TseI GCWGC 3 cut(s) 313, 914, 941
TspDTI ATGAA 8 cut(s) 17, 32, 258, 573, 726, 745, 887, 947
TspRI CASTG 2 cut(s) 160, 196
XapI RAATTY 2 cut(s) 568, 734
XbaI TCTAGA 1 cut(s) 706
XceI RCATGY 2 cut(s) 692, 884
XspI CTAG 2 cut(s) 201, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.