AT5G47730

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006021
Physical Location & Seq
Reverse (-)
19334250 .. 19337148
2899 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G47730.3

Sequence Viewer

Length: 1026 bp
ATGGGGATCGTTTCAGAAGAAGCCATTGATGAATTCCAAGAGCTGATGGATCAAGTTGAGGAGCCACTGAAGAAAACTTATGAGAGAGTCCATCAGGGATATCTAAGGGAGAATTTGGGTCGTTTTCTGAAAGCACGAGACTGGAACGTATGCAAAGCTCACACAATGCTGGTTGAGTGTTTGCGTTGGAGGGTGGATAATGAAATTGACAGTATCCTATCTAAACCAATTGTTCCTACTGAGTTGTATAGGGATGTACGAGATTCTCAACTCATAGGAATGTCAGGTTACACCAAAGAGGGCTTGCCGGTCTTTGCTATTGGCGTCGGCCTCAGCACATTTGACAAAGCTTCTGTTCACTACTATGTCCAATCTCACATACAAATCAATGAATATAGAGACCGTGTTTTACTGCCTTCTATTTCTAAGAAGAATGGGAGGCCAATCACCACCTGCGTCAAGGTTCTAGACATGACGGGGTTGAAGCTTTCAGCTCTAAGCCAAATCAAGTTAGTGACTATCATATCGACCATTGATGATCTCAACTATCCAGAGAAGACAAACACGTACTATGTTGTGAACGCTCCATATATATTTTCCGCATGTTGGAAGGTTGTAAAACCTCTTTTACAAGAGAGGACAAGGAAAAAAGTTCATGTCTTATCCGGTTGCGGAAGGGATGAATTATTGAAGATTATGGACTTCACATCCCTGCCACATTTCTGTAGAAGTGGAAGCTCTGGGTCATCTCACCACACTCAGAGCGCAAATTGTTTTTCTATCAATCATCCCTTCCATCAACAGCTATACAACTATGTGAAGCACCATTATGAAACCCAAGGACAAGCAGAACCTGCAAAGCAAGGATCATTCCACGTGGGTTTTCCTGAGCCTGAAGCTGAACGTTGTGTGATAGCCAAAACCATAGAATCGGAGCTGCACAAGTTTGAGAACCGCAACGGTCTGGCCGTGTCCATTGATGACAGAAAATCCAGTCATGAAACACTGGCAAATGGTGACCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

341

Amino Acids

38.86

Weight (kDa)

6.94

Isoelectric Point (pI)

39.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 34 - 186 4e-19 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 461
Acc36I ACCTGC 2 cut(s) 461, 862
AciI CCGC 3 cut(s) 600, 672, 955
AclI AACGTT 1 cut(s) 904
AclWI GGATC 3 cut(s) 14, 57, 874
AcoI YGGCCR 1 cut(s) 966
AcsI RAATTY 2 cut(s) 32, 112
AcuI CTGAAG 2 cut(s) 89, 915
AcvI CACGTG 1 cut(s) 877
AcyI GRCGYC 1 cut(s) 324
AfaI GTAC 2 cut(s) 258, 569
AfiI CCNNNNNNNGG 1 cut(s) 606
AflIII ACRYGT 1 cut(s) 564
AgsI TTSAA 2 cut(s) 484, 691
AluBI AGCT 9 cut(s) 43, 158, 350, 487, 494, 738, 805, 899, 937
AluI AGCT 9 cut(s) 43, 158, 350, 487, 494, 738, 805, 899, 937
Alw26I GTCTC 2 cut(s) 132, 393
AlwI GGATC 3 cut(s) 14, 57, 874
AlwNI CAGNNNCTG 1 cut(s) 854
AoxI GGCC 3 cut(s) 328, 440, 966
ApeKI GCWGC 1 cut(s) 937
ApoI RAATTY 2 cut(s) 32, 112
AspLEI GCGC 1 cut(s) 767
AsuHPI GGTGA 2 cut(s) 439, 743
BauI CACGAG 1 cut(s) 135
BbrPI CACGTG 1 cut(s) 877
BbsI GAAGAC 1 cut(s) 563
BbvCI CCTCAGC 1 cut(s) 332
BbvI GCAGC 1 cut(s) 924
BccI CCATC 3 cut(s) 40, 99, 804
BceAI ACGGC 1 cut(s) 953
BciVI GTATCC 1 cut(s) 224
BcoDI GTCTC 2 cut(s) 132, 393
BfaI CTAG 1 cut(s) 467
BfmI CTRYAG 1 cut(s) 724
BfuAI ACCTGC 2 cut(s) 461, 862
BfuI GTATCC 1 cut(s) 224
BisI GCNGC 1 cut(s) 938
BlsI GCNGC 1 cut(s) 939
BmiI GGNNCC 1 cut(s) 63
BpiI GAAGAC 1 cut(s) 563
Bpu10I CCTNAGC 2 cut(s) 332, 888
BsaAI YACGTR 2 cut(s) 567, 877
BsaHI GRCGYC 1 cut(s) 324
BsaI GGTCTC 1 cut(s) 393
BsaJI CCNNGG 1 cut(s) 838
BsaWI WCCGGW 1 cut(s) 665
Bsc4I CCNNNNNNNGG 1 cut(s) 606
Bse118I RCCGGY 1 cut(s) 307
Bse1I ACTGG 3 cut(s) 146, 993, 1011
BseDI CCNNGG 1 cut(s) 838
BseGI GGATG 4 cut(s) 259, 685, 707, 787
BseLI CCNNNNNNNGG 1 cut(s) 606
BseMII CTCAG 4 cut(s) 231, 346, 773, 879
BseNI ACTGG 3 cut(s) 146, 993, 1011
BseRI GAGGAG 1 cut(s) 74
BseXI GCAGC 1 cut(s) 924
BsgI GTGCAG 1 cut(s) 923
BshFI GGCC 3 cut(s) 330, 442, 968
BsiSI CCGG 2 cut(s) 308, 666
BslI CCNNNNNNNGG 1 cut(s) 606
BsmAI GTCTC 2 cut(s) 132, 393
BsnI GGCC 3 cut(s) 330, 442, 968
Bso31I GGTCTC 1 cut(s) 393
Bsp143I GATC 4 cut(s) 6, 49, 538, 866
BspACI CCGC 3 cut(s) 600, 672, 955
BspANI GGCC 3 cut(s) 330, 442, 968
BspCNI CTCAG 4 cut(s) 232, 345, 772, 880
BspHI TCATGA 1 cut(s) 997
BspLI GGNNCC 1 cut(s) 63
BspMI ACCTGC 2 cut(s) 461, 862
BspPI GGATC 3 cut(s) 14, 57, 874
BspTNI GGTCTC 1 cut(s) 393
BsrFI RCCGGY 1 cut(s) 307
BsrI ACTGG 3 cut(s) 146, 993, 1011
BssAI RCCGGY 1 cut(s) 307
BssECI CCNNGG 1 cut(s) 838
BssMI GATC 4 cut(s) 6, 49, 538, 866
BssNI GRCGYC 1 cut(s) 324
BssSI CACGAG 1 cut(s) 135
BssT1I CCWWGG 1 cut(s) 838
Bst2BI CACGAG 1 cut(s) 135
Bst4CI ACNGT 3 cut(s) 212, 404, 962
BstACI GRCGYC 1 cut(s) 324
BstAPI GCANNNNNTGC 1 cut(s) 854
BstBAI YACGTR 2 cut(s) 567, 877
BstC8I GCNNGC 1 cut(s) 305
BstDEI CTNAG 7 cut(s) 104, 240, 332, 426, 497, 759, 888
BstEII GGTNACC 1 cut(s) 1016
BstF5I GGATG 4 cut(s) 259, 685, 707, 787
BstHHI GCGC 1 cut(s) 767
BstKTI GATC 4 cut(s) 9, 52, 541, 869
BstMAI GTCTC 2 cut(s) 132, 393
BstMBI GATC 4 cut(s) 6, 49, 538, 866
BstMWI GCNNNNNNNGC 1 cut(s) 854
BstNSI RCATGY 1 cut(s) 606
BstPI GGTNACC 1 cut(s) 1016
BstSFI CTRYAG 1 cut(s) 724
BstV1I GCAGC 1 cut(s) 924
BstV2I GAAGAC 1 cut(s) 563
BsuI GTATCC 1 cut(s) 224
BsuRI GGCC 3 cut(s) 330, 442, 968
BtsCI GGATG 4 cut(s) 259, 685, 707, 787
BtsIMutI CAGTG 2 cut(s) 65, 1004
BveI ACCTGC 2 cut(s) 461, 862
Cac8I GCNNGC 1 cut(s) 305
CaiI CAGNNNCTG 1 cut(s) 854
CciI TCATGA 1 cut(s) 997
CfoI GCGC 1 cut(s) 767
Cfr10I RCCGGY 1 cut(s) 307
CseI GACGC 2 cut(s) 313, 445
Csp6I GTAC 2 cut(s) 257, 568
CviAII CATG 4 cut(s) 472, 603, 656, 998
CviQI GTAC 2 cut(s) 257, 568
DdeI CTNAG 7 cut(s) 104, 240, 332, 426, 497, 759, 888
DpnI GATC 4 cut(s) 8, 51, 540, 868
DpnII GATC 4 cut(s) 6, 49, 538, 866
EaeI YGGCCR 1 cut(s) 966
Eco130I CCWWGG 1 cut(s) 838
Eco31I GGTCTC 1 cut(s) 393
Eco32I GATATC 1 cut(s) 101
Eco57I CTGAAG 2 cut(s) 89, 915
Eco72I CACGTG 1 cut(s) 877
Eco91I GGTNACC 1 cut(s) 1016
EcoO65I GGTNACC 1 cut(s) 1016
EcoRI GAATTC 1 cut(s) 32
EcoRV GATATC 1 cut(s) 101
EcoT14I CCWWGG 1 cut(s) 838
ErhI CCWWGG 1 cut(s) 838
FaeI CATG 4 cut(s) 475, 606, 659, 1001
FatI CATG 4 cut(s) 471, 602, 655, 997
Fnu4HI GCNGC 1 cut(s) 938
FokI GGATG 4 cut(s) 266, 692, 694, 774
Fsp4HI GCNGC 1 cut(s) 938
FspBI CTAG 1 cut(s) 467
GlaI GCGC 1 cut(s) 766
GluI GCNGC 1 cut(s) 938
HaeIII GGCC 3 cut(s) 330, 442, 968
HapII CCGG 2 cut(s) 308, 666
HgaI GACGC 2 cut(s) 313, 445
HhaI GCGC 1 cut(s) 767
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 4 cut(s) 475, 606, 659, 1001
Hin6I GCGC 1 cut(s) 765
HinP1I GCGC 1 cut(s) 765
HindIII AAGCTT 2 cut(s) 348, 485
HinfI GANTC 3 cut(s) 87, 263, 929
HpaII CCGG 2 cut(s) 308, 666
HphI GGTGA 2 cut(s) 439, 743
Hpy166II GTNNAC 2 cut(s) 358, 580
Hpy188I TCNGA 5 cut(s) 16, 129, 762, 934, 1025
Hpy188III TCNNGA 4 cut(s) 467, 551, 887, 998
Hpy8I GTNNAC 2 cut(s) 358, 580
Hpy99I CGWCG 1 cut(s) 329
HpyAV CCTTC 4 cut(s) 426, 604, 669, 802
HpyCH4III ACNGT 3 cut(s) 212, 404, 962
HpyCH4IV ACGT 4 cut(s) 147, 566, 876, 904
HpyCH4V TGCA 3 cut(s) 153, 857, 940
HpyF10VI GCNNNNNNNGC 1 cut(s) 854
HpyF3I CTNAG 7 cut(s) 104, 240, 332, 426, 497, 759, 888
HpySE526I ACGT 4 cut(s) 147, 566, 876, 904
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 4 cut(s) 475, 606, 659, 1001
HspAI GCGC 1 cut(s) 765
Kzo9I GATC 4 cut(s) 6, 49, 538, 866
LmnI GCTCC 3 cut(s) 61, 589, 934
Lsp1109I GCAGC 1 cut(s) 924
MaeI CTAG 1 cut(s) 467
MaeII ACGT 4 cut(s) 147, 566, 876, 904
MaeIII GTNAC 3 cut(s) 287, 514, 1016
MalI GATC 4 cut(s) 8, 51, 540, 868
MboI GATC 4 cut(s) 6, 49, 538, 866
MboII GAAGA 5 cut(s) 29, 82, 442, 568, 703
MfeI CAATTG 1 cut(s) 228
MluCI AATT 6 cut(s) 32, 112, 204, 228, 683, 769
MlyI GAGTC 1 cut(s) 96
MmeI TCCRAC 2 cut(s) 167, 587
MnlI CCTC 7 cut(s) 52, 183, 292, 341, 432, 630, 633
MslI CAYNNNNRTG 3 cut(s) 278, 363, 828
MspI CCGG 2 cut(s) 308, 666
MunI CAATTG 1 cut(s) 228
MwoI GCNNNNNNNGC 1 cut(s) 854
NdeII GATC 4 cut(s) 6, 49, 538, 866
NlaIII CATG 4 cut(s) 475, 606, 659, 1001
NlaIV GGNNCC 1 cut(s) 63
NmuCI GTSAC 2 cut(s) 514, 1016
NspI RCATGY 1 cut(s) 606
PagI TCATGA 1 cut(s) 997
PaqCI CACCTGC 1 cut(s) 461
PcsI WCGNNNNNNNCGW 1 cut(s) 966
PfeI GAWTC 2 cut(s) 263, 929
PkrI GCNGC 1 cut(s) 939
PleI GAGTC 1 cut(s) 95
PmaCI CACGTG 1 cut(s) 877
PmlI CACGTG 1 cut(s) 877
PpsI GAGTC 1 cut(s) 95
Ppu21I YACGTR 2 cut(s) 567, 877
Psp1406I AACGTT 1 cut(s) 904
PspCI CACGTG 1 cut(s) 877
PspEI GGTNACC 1 cut(s) 1016
PspN4I GGNNCC 1 cut(s) 63
PstNI CAGNNNCTG 1 cut(s) 854
RsaI GTAC 2 cut(s) 258, 569
RsaNI GTAC 2 cut(s) 257, 568
RseI CAYNNNNRTG 3 cut(s) 278, 363, 828
SatI GCNGC 1 cut(s) 938
Sau3AI GATC 4 cut(s) 6, 49, 538, 866
SchI GAGTC 1 cut(s) 96
SfcI CTRYAG 1 cut(s) 724
SmiMI CAYNNNNRTG 3 cut(s) 278, 363, 828
Sse9I AATT 6 cut(s) 32, 112, 204, 228, 683, 769
SsiI CCGC 3 cut(s) 600, 672, 955
SspMI CTAG 1 cut(s) 467
StyI CCWWGG 1 cut(s) 838
TaaI ACNGT 3 cut(s) 212, 404, 962
TaiI ACGT 4 cut(s) 150, 569, 879, 907
TaqI TCGA 1 cut(s) 527
TasI AATT 6 cut(s) 32, 112, 204, 228, 683, 769
TfiI GAWTC 2 cut(s) 263, 929
TscAI CASTG 2 cut(s) 72, 1011
TseFI GTSAC 2 cut(s) 514, 1016
TseI GCWGC 1 cut(s) 937
Tsp45I GTSAC 2 cut(s) 514, 1016
TspDTI ATGAA 7 cut(s) 45, 216, 405, 644, 696, 846, 1014
TspRI CASTG 2 cut(s) 72, 1011
XapI RAATTY 2 cut(s) 32, 112
XbaI TCTAGA 1 cut(s) 466
XceI RCATGY 1 cut(s) 606
XspI CTAG 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.