Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0006021

Physical Location & Seq

Genomic Coordinates Reverse (-)

41531263 .. 41534767

Gene Structure 3505 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1339800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1011 bp

ATGGGGATCGTTTCTCAGGAAGCGATCAAGCAGTTCCAAGCTCTGATGGATCAAGTTGATGAGCCTCTCAAGAGAACGTTTCAGAATGTTCATCAAGGCTATCTAGTTGAGACTTTCGGCCGTTTTCTCAAAGCAAGGGATTCCAACGTTGCCAAAGCCCATAAAATGTTGGTCGATTGTTTAAACTGGAGGGTACAAAACGATATCGACAATATTTTGGCCAAGCCCATAGTTCCAACTGAACTATACAGAGGAGTGCGAGATTCACAGCTCATAGGTCTTTCGGGTTACTCAAGAGAGGGCTTGCCCATCTTTGCCATTGGTGTTGGACTAAGTACATTTGACAAAGCATCAGTTCACTACTATGTTCAGTCACACATTCAAATTAATGAATATCGAGATCGTGTAATTTTGCCTTCTGCATCGAAGAAGTATGGTAAGCATATTACCACCTGCATCAAGGTTTTAGATATGACTGGTTTGAAGCTCTCAGCACTAAGCCAAATAAAGTTAATGACAATTATATCAACCATTGATGATTTGAACTACCCGGAGAGGACAAATACATACTACATTGTAAATGCCCCATATATATTCTCAGCTTGTTGGAAGGTTGTCAAGCCACTTTTGCAGGAGAGGACAAGGAAAAAAGTGCAAGTGTTGTCTGGTTGTGGTAGGGATGAACTGTTGAAGATAATGGATTATGCATCTCTTCCACATTTCTGTAAAAGTGAAGGTTCGGGATCATCTCGAAATTCACAGAATGGAGCTGACAATTGCTTCTCCCTGGACCACCACTTTCATCAACAGCTCTACAACCACATCAAGCAGCAATCGATGATGAAGGAATCCGTTCAACCATTCAGACACGGTTCTTTTCATGTGGATGTACCTGAGGCAGCTGCAGAAAGAACACAGATTGCTAAAACGATAGAATCCGAGTTTAACAAATTTGGTAATGGGAATGGGCTATCTGAATCTATAGATGGCCTCAAAATCAATGGTGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CRAL_TRIO CRAL_TRIO_N

Protein Analysis

337

Amino Acids

37.96

Weight (kDa)

8.74

Isoelectric Point (pI)

35.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CRAL_TRIO_N	PF03765	8 - 59	4.9e-09	CRAL/TRIO, N-terminal domain
CRAL_TRIO	PF00650	89 - 241	9.7e-19	CRAL/TRIO domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006021)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G47730 AT5G47730 AT5G47730 AT5G47730
fragaria_vesca	FvH4_3g00280 FvH4_3g00280 FvH4_3g00280 FvH4_4g17420
malus_domestica	MD05G1362300.v1.1 MD10G1339800.v1.1
prunus_persica	Prupe.4G003100_v2.0.a1 Prupe.4G003100_v2.0.a1 Prupe.4G003100_v2.0.a1 Prupe.4G003100_v2.0.a1
pyrus_communis	pycom05g32870 pycom10g28960
rosa_chinensis	RchiOBHm_Chr5g0000411
rosa_laevigata	RLG00000030864
rosa_multiflora	Rmu_co8373963.1_g000001 Rmu_sc0003949.1_g000001
rosa_roxburghii	Rroxscaffold_1G00075750
rosa_rugosa	Rorug04G0383400
rosa_samantha	Rh5AG003300 Rh5BG004400 Rh5CG003500 Rh5DG003400
rosa_wichuraiana	Rw5G000330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	461
Acc36I	ACCTGC	1 cut(s)	461
AclI	AACGTT	2 cut(s)	77, 147
AclWI	GGATC	3 cut(s)	14, 57, 751
AcoI	YGGCCR	2 cut(s)	118, 219
AcsI	RAATTY	2 cut(s)	754, 950
AfaI	GTAC	3 cut(s)	195, 337, 891
AgsI	TTSAA	5 cut(s)	383, 484, 544, 691, 857
AjnI	CCWGG	1 cut(s)	786
AluBI	AGCT	7 cut(s)	41, 271, 487, 602, 770, 811, 902
AluI	AGCT	7 cut(s)	41, 271, 487, 602, 770, 811, 902
Alw26I	GTCTC	1 cut(s)	104
AlwI	GGATC	3 cut(s)	14, 57, 751
AoxI	GGCC	3 cut(s)	118, 219, 988
ApeKI	GCWGC	3 cut(s)	829, 899, 902
ApoI	RAATTY	2 cut(s)	754, 950
AseI	ATTAAT	1 cut(s)	387
Asp700I	GAANNNNTTC	1 cut(s)	852
AspS9I	GGNCC	1 cut(s)	790
AsuC2I	CCSGG	1 cut(s)	551
AvaII	GGWCC	1 cut(s)	790
AxyI	CCTNAGG	1 cut(s)	894
BalI	TGGCCA	1 cut(s)	221
BbvI	GCAGC	3 cut(s)	841, 889, 911
BccI	CCATC	3 cut(s)	40, 317, 980
BceAI	ACGGC	1 cut(s)	105
BciT130I	CCWGG	1 cut(s)	788
BcnI	CCSGG	1 cut(s)	551
BcoDI	GTCTC	1 cut(s)	104
BfaI	CTAG	1 cut(s)	104
BfmI	CTRYAG	2 cut(s)	903, 981
BfuAI	ACCTGC	1 cut(s)	461
BisI	GCNGC	3 cut(s)	830, 900, 903
BlsI	GCNGC	3 cut(s)	831, 901, 904
Bme1390I	CCNGG	2 cut(s)	551, 788
Bme18I	GGWCC	1 cut(s)	790
BmgT120I	GGNCC	1 cut(s)	790
BmrFI	CCNGG	2 cut(s)	551, 788
BmsI	GCATC	4 cut(s)	359, 431, 465, 716
BpmI	CTGGAG	1 cut(s)	208
BpuEI	CTTGAG	2 cut(s)	53, 277
BpuMI	CCSGG	1 cut(s)	551
Bsa29I	ATCGAT	1 cut(s)	836
BsaJI	CCNNGG	1 cut(s)	786
Bse1I	ACTGG	2 cut(s)	191, 481
Bse21I	CCTNAGG	1 cut(s)	894
BseBI	CCWGG	1 cut(s)	788
BseCI	ATCGAT	1 cut(s)	836
BseDI	CCNNGG	1 cut(s)	786
BseGI	GGATG	2 cut(s)	685, 892
BseMII	CTCAG	4 cut(s)	29, 504, 612, 885
BseNI	ACTGG	2 cut(s)	191, 481
BseRI	GAGGAG	1 cut(s)	267
BseX3I	CGGCCG	1 cut(s)	118
BseXI	GCAGC	3 cut(s)	841, 889, 911
Bsh1285I	CGRYCG	1 cut(s)	121
BshFI	GGCC	3 cut(s)	120, 221, 990
BshVI	ATCGAT	1 cut(s)	836
BsiEI	CGRYCG	1 cut(s)	121
BsiSI	CCGG	1 cut(s)	551
BsmAI	GTCTC	1 cut(s)	104
BsnI	GGCC	3 cut(s)	120, 221, 990
Bsp143I	GATC	5 cut(s)	6, 24, 49, 400, 743
BspANI	GGCC	3 cut(s)	120, 221, 990
BspCNI	CTCAG	4 cut(s)	28, 503, 611, 886
BspDI	ATCGAT	1 cut(s)	836
BspMAI	CTGCAG	1 cut(s)	907
BspMI	ACCTGC	1 cut(s)	461
BspPI	GGATC	3 cut(s)	14, 57, 751
BsrI	ACTGG	2 cut(s)	191, 481
BssECI	CCNNGG	1 cut(s)	786
BssMI	GATC	5 cut(s)	6, 24, 49, 400, 743
Bst2UI	CCWGG	1 cut(s)	788
Bst4CI	ACNGT	2 cut(s)	687, 872
Bst6I	CTCTTC	1 cut(s)	717
BstC8I	GCNNGC	1 cut(s)	305
BstDEI	CTNAG	6 cut(s)	15, 332, 490, 497, 598, 894
BstF5I	GGATG	2 cut(s)	685, 892
BstKTI	GATC	5 cut(s)	9, 27, 52, 403, 746
BstMAI	GTCTC	1 cut(s)	104
BstMBI	GATC	5 cut(s)	6, 24, 49, 400, 743
BstMCI	CGRYCG	1 cut(s)	121
BstMWI	GCNNNNNNNGC	1 cut(s)	628
BstNI	CCWGG	1 cut(s)	788
BstSCI	CCNGG	2 cut(s)	549, 786
BstSFI	CTRYAG	2 cut(s)	903, 981
BstV1I	GCAGC	3 cut(s)	841, 889, 911
BstZI	CGGCCG	1 cut(s)	118
Bsu15I	ATCGAT	1 cut(s)	836
Bsu36I	CCTNAGG	1 cut(s)	894
BsuRI	GGCC	3 cut(s)	120, 221, 990
BsuTUI	ATCGAT	1 cut(s)	836
BtsCI	GGATG	2 cut(s)	685, 892
BveI	ACCTGC	1 cut(s)	461
Cac8I	GCNNGC	1 cut(s)	305
Cfr13I	GGNCC	1 cut(s)	790
ClaI	ATCGAT	1 cut(s)	836
Csp6I	GTAC	3 cut(s)	194, 336, 890
CviAII	CATG	1 cut(s)	881
CviQI	GTAC	3 cut(s)	194, 336, 890
DdeI	CTNAG	6 cut(s)	15, 332, 490, 497, 598, 894
DpnI	GATC	5 cut(s)	8, 26, 51, 402, 745
DpnII	GATC	5 cut(s)	6, 24, 49, 400, 743
DraI	TTTAAA	1 cut(s)	183
EaeI	YGGCCR	2 cut(s)	118, 219
EagI	CGGCCG	1 cut(s)	118
Eam1104I	CTCTTC	1 cut(s)	717
EarI	CTCTTC	1 cut(s)	717
EclXI	CGGCCG	1 cut(s)	118
Eco32I	GATATC	1 cut(s)	205
Eco47I	GGWCC	1 cut(s)	790
Eco52I	CGGCCG	1 cut(s)	118
Eco81I	CCTNAGG	1 cut(s)	894
EcoRII	CCWGG	1 cut(s)	786
EcoRV	GATATC	1 cut(s)	205
EcoT22I	ATGCAT	1 cut(s)	709
FaeI	CATG	1 cut(s)	884
FatI	CATG	1 cut(s)	880
Fnu4HI	GCNGC	3 cut(s)	830, 900, 903
FokI	GGATG	2 cut(s)	692, 899
Fsp4HI	GCNGC	3 cut(s)	830, 900, 903
FspBI	CTAG	1 cut(s)	104
GluI	GCNGC	3 cut(s)	830, 900, 903
GsuI	CTGGAG	1 cut(s)	208
HaeIII	GGCC	3 cut(s)	120, 221, 990
HapII	CCGG	1 cut(s)	551
Hin1II	CATG	1 cut(s)	884
HinfI	GANTC	5 cut(s)	140, 263, 848, 935, 977
HpaII	CCGG	1 cut(s)	551
Hpy166II	GTNNAC	1 cut(s)	358
Hpy188I	TCNGA	5 cut(s)	45, 84, 866, 940, 976
Hpy188III	TCNNGA	6 cut(s)	17, 70, 294, 398, 741, 750
Hpy8I	GTNNAC	1 cut(s)	358
HpyAV	CCTTC	4 cut(s)	426, 604, 728, 838
HpyCH4III	ACNGT	2 cut(s)	687, 872
HpyCH4IV	ACGT	2 cut(s)	77, 147
HpyCH4V	TGCA	6 cut(s)	422, 456, 631, 655, 707, 905
HpyF10VI	GCNNNNNNNGC	1 cut(s)	628
HpyF3I	CTNAG	6 cut(s)	15, 332, 490, 497, 598, 894
HpySE526I	ACGT	2 cut(s)	77, 147
Hsp92II	CATG	1 cut(s)	884
Kzo9I	GATC	5 cut(s)	6, 24, 49, 400, 743
LmnI	GCTCC	1 cut(s)	767
Lsp1109I	GCAGC	3 cut(s)	841, 889, 911
LweI	GCATC	4 cut(s)	359, 431, 465, 716
MaeI	CTAG	1 cut(s)	104
MaeII	ACGT	2 cut(s)	77, 147
MaeIII	GTNAC	3 cut(s)	287, 372, 1004
MalI	GATC	5 cut(s)	8, 26, 51, 402, 745
MboI	GATC	5 cut(s)	6, 24, 49, 400, 743
MboII	GAAGA	3 cut(s)	439, 703, 704
MfeI	CAATTG	1 cut(s)	775
MlsI	TGGCCA	1 cut(s)	221
MluCI	AATT	6 cut(s)	384, 408, 519, 754, 775, 950
MluNI	TGGCCA	1 cut(s)	221
MmeI	TCCRAC	4 cut(s)	168, 260, 307, 587
MnlI	CCTC	8 cut(s)	75, 183, 245, 292, 549, 630, 889, 1001
Mox20I	TGGCCA	1 cut(s)	221
Mph1103I	ATGCAT	1 cut(s)	709
MroXI	GAANNNNTTC	1 cut(s)	852
MscI	TGGCCA	1 cut(s)	221
MseI	TTAA	4 cut(s)	182, 387, 512, 945
MslI	CAYNNNNRTG	2 cut(s)	363, 885
Msp20I	TGGCCA	1 cut(s)	221
MspA1I	CMGCKG	1 cut(s)	902
MspI	CCGG	1 cut(s)	551
MspR9I	CCNGG	2 cut(s)	551, 788
MssI	GTTTAAAC	1 cut(s)	183
MunI	CAATTG	1 cut(s)	775
MvaI	CCWGG	1 cut(s)	788
MwoI	GCNNNNNNNGC	1 cut(s)	628
NciI	CCSGG	1 cut(s)	551
NdeII	GATC	5 cut(s)	6, 24, 49, 400, 743
NlaIII	CATG	1 cut(s)	884
NmuCI	GTSAC	2 cut(s)	372, 1004
NsiI	ATGCAT	1 cut(s)	709
PaqCI	CACCTGC	1 cut(s)	461
PdmI	GAANNNNTTC	1 cut(s)	852
PfeI	GAWTC	5 cut(s)	140, 263, 848, 935, 977
PkrI	GCNGC	3 cut(s)	831, 901, 904
PmeI	GTTTAAAC	1 cut(s)	183
PshBI	ATTAAT	1 cut(s)	387
Psp1406I	AACGTT	2 cut(s)	77, 147
Psp6I	CCWGG	1 cut(s)	786
PspGI	CCWGG	1 cut(s)	786
PspPI	GGNCC	1 cut(s)	790
PstI	CTGCAG	1 cut(s)	907
PvuII	CAGCTG	1 cut(s)	902
RsaI	GTAC	3 cut(s)	195, 337, 891
RsaNI	GTAC	3 cut(s)	194, 336, 890
RseI	CAYNNNNRTG	2 cut(s)	363, 885
SaqAI	TTAA	4 cut(s)	182, 387, 512, 945
SatI	GCNGC	3 cut(s)	830, 900, 903
Sau3AI	GATC	5 cut(s)	6, 24, 49, 400, 743
Sau96I	GGNCC	1 cut(s)	790
ScrFI	CCNGG	2 cut(s)	551, 788
SfaNI	GCATC	4 cut(s)	359, 431, 465, 716
SfcI	CTRYAG	2 cut(s)	903, 981
SinI	GGWCC	1 cut(s)	790
SmiMI	CAYNNNNRTG	2 cut(s)	363, 885
SmlI	CTYRAG	2 cut(s)	68, 292
SmoI	CTYRAG	2 cut(s)	68, 292
Sse9I	AATT	6 cut(s)	384, 408, 519, 754, 775, 950
SspI	AATATT	1 cut(s)	214
SspMI	CTAG	1 cut(s)	104
StyD4I	CCNGG	2 cut(s)	549, 786
TaaI	ACNGT	2 cut(s)	687, 872
TaiI	ACGT	2 cut(s)	80, 150
TaqI	TCGA	6 cut(s)	174, 207, 397, 425, 751, 836
TasI	AATT	6 cut(s)	384, 408, 519, 754, 775, 950
TatI	WGTACW	1 cut(s)	335
TfiI	GAWTC	5 cut(s)	140, 263, 848, 935, 977
Tru1I	TTAA	4 cut(s)	182, 387, 512, 945
Tru9I	TTAA	4 cut(s)	182, 387, 512, 945
TseFI	GTSAC	2 cut(s)	372, 1004
TseI	GCWGC	3 cut(s)	829, 899, 902
Tsp45I	GTSAC	2 cut(s)	372, 1004
TspDTI	ATGAA	6 cut(s)	80, 405, 696, 791, 857, 869
TspGWI	ACGGA	1 cut(s)	841
VpaK11BI	GGWCC	1 cut(s)	790
VspI	ATTAAT	1 cut(s)	387
XapI	RAATTY	2 cut(s)	754, 950
XmnI	GAANNNNTTC	1 cut(s)	852
XspI	CTAG	1 cut(s)	104
Zsp2I	ATGCAT	1 cut(s)	709

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.