Rroxscaffold_1G00075750

transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006021
Physical Location & Seq
Reverse (-)
96366300 .. 96369664
3365 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00075750.1

Sequence Viewer

Length: 945 bp
ATGCCAGTTGATGAACCACTGAAGAGAACATTTCAGTTGATGGATTGTTTAAACTGGAGGGTACAAAATGAGATTGACAGTATATTGGCCAAACCCATAATTCCAACTGAATTGTACAGAGGAGTGCGAGACTCACAGCTCTTAGGCCTTTCAGGTTACTCTAGAGAGGTGCTCATTCTTACTGAAACCAGTCCTGTTTTTGATCTTTACAGTTTTCTCACTATCCCTGACACTGGTATGCCAGTCTTTGCTATTGGGGTTGGGTTAAGTACATTTAATAAAGCGTCTGTTCATTACTATGTGCAGTCACACATTCAAATCAATGAATATCGAGATCGTGTTATTTTGCCATCTGCATCGAAGAAGTATGGTAAGCCTATTACCACGTGTGTGAAGGTTTTAGATATGACTGGGTTGAAGCTTTCAGCACTAAACCAAATTAAGTTGTTGACGATTATATCAACAGTTGATGATTTGAACTACCCAGAGAAGACAAATACGTACTACATTGTAAATGCGCCTTACATATTCTCAGCATGTTGGAAGGTTGTCAAGCCCCTTTTGCAGGAGAGGACAAGAAAAAAAGTACAAGTATTGTCTGGTTGTGGTACAGATGAGTTGTTGAAGATAATGGATTATGCATCTCTTCCCCATTTCTGTAAAAGGGAAGGTTCTGGATCATCTCAGCATTCACAGAATGGAACTGACAATTGCTTCTCCTTGGATCACCATTTTCATCAACAGCTCTACAACTACATCAAGCAGCAATCTTTGATCAATGAATCCGTTCAACCAATCAAGCAGGGTTCTTTTCATGTGGACATACCTGAGCCAGCTGCAGAAGGAACACCGATTGCCAAAACAATAGAATCTGAGTTCAACAAGTTTGGTAACGGCAATGGGGTATCTGACTCTCTAAATGCCCTCAAAATCAATGGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

314

Amino Acids

35.23

Weight (kDa)

6.71

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 120 - 219 8.6e-17 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 685, 732
AcoI YGGCCR 1 cut(s) 87
AcuI CTGAAG 1 cut(s) 41
AcvI CACGTG 1 cut(s) 387
AfaI GTAC 6 cut(s) 63, 116, 271, 503, 588, 610
AfiI CCNNNNNNNGG 2 cut(s) 233, 565
AflIII ACRYGT 1 cut(s) 386
AgsI TTSAA 6 cut(s) 317, 418, 478, 625, 791, 880
AleI CACNNNNGTG 1 cut(s) 389
AluBI AGCT 4 cut(s) 139, 421, 745, 836
AluI AGCT 4 cut(s) 139, 421, 745, 836
Alw21I GWGCWC 1 cut(s) 174
Alw26I GTCTC 1 cut(s) 123
AlwI GGATC 2 cut(s) 685, 732
AoxI GGCC 2 cut(s) 87, 145
ApeKI GCWGC 2 cut(s) 763, 836
Asp700I GAANNNNTTC 1 cut(s) 786
AspLEI GCGC 1 cut(s) 520
AsuHPI GGTGA 1 cut(s) 719
BalI TGGCCA 1 cut(s) 89
BbrPI CACGTG 1 cut(s) 387
BbsI GAAGAC 1 cut(s) 497
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 2 cut(s) 775, 823
BccI CCATC 2 cut(s) 34, 358
BceAI ACGGC 1 cut(s) 910
BclI TGATCA 1 cut(s) 774
BcoDI GTCTC 1 cut(s) 123
BfaI CTAG 1 cut(s) 162
BfmI CTRYAG 1 cut(s) 837
BisI GCNGC 2 cut(s) 764, 837
BlsI GCNGC 2 cut(s) 765, 838
BmrI ACTGGG 1 cut(s) 420
BmsI GCATC 2 cut(s) 365, 650
BmuI ACTGGG 1 cut(s) 420
BpiI GAAGAC 1 cut(s) 497
BplI GAGNNNNNCTC 2 cut(s) 156, 188
BpmI CTGGAG 1 cut(s) 76
Bpu10I CCTNAGC 1 cut(s) 828
BsaAI YACGTR 2 cut(s) 387, 501
BsaJI CCNNGG 1 cut(s) 720
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 565
Bse1I ACTGG 6 cut(s) 5, 59, 189, 238, 242, 415
Bse3DI GCAATG 1 cut(s) 904
BseDI CCNNGG 1 cut(s) 720
BseLI CCNNNNNNNGG 2 cut(s) 233, 565
BseMI GCAATG 1 cut(s) 904
BseMII CTCAG 4 cut(s) 546, 698, 819, 864
BseNI ACTGG 6 cut(s) 5, 59, 189, 238, 242, 415
BseRI GAGGAG 1 cut(s) 135
BseXI GCAGC 2 cut(s) 775, 823
BsgI GTGCAG 1 cut(s) 323
BshFI GGCC 2 cut(s) 89, 147
BsiHKAI GWGCWC 1 cut(s) 174
BslI CCNNNNNNNGG 2 cut(s) 233, 565
BsmAI GTCTC 1 cut(s) 123
BsmI GAATGC 1 cut(s) 688
BsnI GGCC 2 cut(s) 89, 147
Bsp1286I GDGCHC 1 cut(s) 174
Bsp1407I TGTACA 1 cut(s) 114
Bsp143I GATC 5 cut(s) 202, 334, 677, 724, 774
BspANI GGCC 2 cut(s) 89, 147
BspCNI CTCAG 4 cut(s) 545, 697, 820, 865
BspMAI CTGCAG 1 cut(s) 841
BspPI GGATC 2 cut(s) 685, 732
BsrDI GCAATG 1 cut(s) 904
BsrGI TGTACA 1 cut(s) 114
BsrI ACTGG 6 cut(s) 5, 59, 189, 238, 242, 415
BssECI CCNNGG 1 cut(s) 720
BssMI GATC 5 cut(s) 202, 334, 677, 724, 774
BssT1I CCWWGG 1 cut(s) 720
Bst4CI ACNGT 3 cut(s) 80, 212, 466
Bst6I CTCTTC 2 cut(s) 17, 651
BstAUI TGTACA 1 cut(s) 114
BstBAI YACGTR 2 cut(s) 387, 501
BstC8I GCNNGC 1 cut(s) 834
BstDEI CTNAG 5 cut(s) 142, 532, 684, 828, 873
BstENI CCTNNNNNAGG 1 cut(s) 563
BstHHI GCGC 1 cut(s) 520
BstKTI GATC 5 cut(s) 205, 337, 680, 727, 777
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 5 cut(s) 202, 334, 677, 724, 774
BstMWI GCNNNNNNNGC 1 cut(s) 562
BstNSI RCATGY 1 cut(s) 540
BstSFI CTRYAG 1 cut(s) 837
BstSNI TACGTA 1 cut(s) 501
BstV1I GCAGC 2 cut(s) 775, 823
BstV2I GAAGAC 1 cut(s) 497
BsuRI GGCC 2 cut(s) 89, 147
BtsIMutI CAGTG 2 cut(s) 17, 231
Cac8I GCNNGC 1 cut(s) 834
CfoI GCGC 1 cut(s) 520
CseI GACGC 1 cut(s) 273
Csp6I GTAC 6 cut(s) 62, 115, 270, 502, 587, 609
CviAII CATG 2 cut(s) 537, 815
CviJI RGCY 9 cut(s) 89, 139, 147, 376, 421, 556, 745, 832, 836
CviKI_1 RGCY 9 cut(s) 89, 139, 147, 376, 421, 556, 745, 832, 836
CviQI GTAC 6 cut(s) 62, 115, 270, 502, 587, 609
DdeI CTNAG 5 cut(s) 142, 532, 684, 828, 873
DpnI GATC 5 cut(s) 204, 336, 679, 726, 776
DpnII GATC 5 cut(s) 202, 334, 677, 724, 774
DraI TTTAAA 1 cut(s) 51
EaeI YGGCCR 1 cut(s) 87
Eam1104I CTCTTC 2 cut(s) 17, 651
EarI CTCTTC 2 cut(s) 17, 651
Eco105I TACGTA 1 cut(s) 501
Eco130I CCWWGG 1 cut(s) 720
Eco147I AGGCCT 1 cut(s) 147
Eco57I CTGAAG 1 cut(s) 41
Eco72I CACGTG 1 cut(s) 387
EcoNI CCTNNNNNAGG 1 cut(s) 563
EcoT14I CCWWGG 1 cut(s) 720
EcoT22I ATGCAT 1 cut(s) 643
ErhI CCWWGG 1 cut(s) 720
FaeI CATG 2 cut(s) 540, 818
FatI CATG 2 cut(s) 536, 814
FbaI TGATCA 1 cut(s) 774
Fnu4HI GCNGC 2 cut(s) 764, 837
Fsp4HI GCNGC 2 cut(s) 764, 837
FspBI CTAG 1 cut(s) 162
GlaI GCGC 1 cut(s) 519
GluI GCNGC 2 cut(s) 764, 837
GsuI CTGGAG 1 cut(s) 76
HaeIII GGCC 2 cut(s) 89, 147
HgaI GACGC 1 cut(s) 273
HhaI GCGC 1 cut(s) 520
Hin1II CATG 2 cut(s) 540, 818
Hin6I GCGC 1 cut(s) 518
HinP1I GCGC 1 cut(s) 518
HincII GTYRAC 1 cut(s) 450
HindII GTYRAC 1 cut(s) 450
HindIII AAGCTT 1 cut(s) 419
HinfI GANTC 4 cut(s) 131, 782, 869, 911
HphI GGTGA 1 cut(s) 719
Hpy166II GTNNAC 2 cut(s) 450, 820
Hpy188I TCNGA 2 cut(s) 874, 910
Hpy188III TCNNGA 3 cut(s) 162, 332, 675
Hpy8I GTNNAC 2 cut(s) 450, 820
HpyAV CCTTC 4 cut(s) 388, 538, 662, 836
HpyCH4III ACNGT 3 cut(s) 80, 212, 466
HpyCH4IV ACGT 2 cut(s) 386, 500
HpyCH4V TGCA 5 cut(s) 304, 356, 565, 641, 839
HpyF10VI GCNNNNNNNGC 1 cut(s) 562
HpyF3I CTNAG 5 cut(s) 142, 532, 684, 828, 873
HpySE526I ACGT 2 cut(s) 386, 500
Hsp92II CATG 2 cut(s) 540, 818
HspAI GCGC 1 cut(s) 518
Ksp22I TGATCA 1 cut(s) 774
Kzo9I GATC 5 cut(s) 202, 334, 677, 724, 774
Lsp1109I GCAGC 2 cut(s) 775, 823
LweI GCATC 2 cut(s) 365, 650
MaeI CTAG 1 cut(s) 162
MaeII ACGT 2 cut(s) 386, 500
MaeIII GTNAC 4 cut(s) 155, 306, 890, 938
MalI GATC 5 cut(s) 204, 336, 679, 726, 776
MboI GATC 5 cut(s) 202, 334, 677, 724, 774
MboII GAAGA 5 cut(s) 34, 373, 502, 637, 638
MfeI CAATTG 1 cut(s) 709
MhlI GDGCHC 1 cut(s) 174
MlsI TGGCCA 1 cut(s) 89
MluCI AATT 4 cut(s) 99, 110, 438, 709
MluNI TGGCCA 1 cut(s) 89
MlyI GAGTC 2 cut(s) 125, 905
MmeI TCCRAC 2 cut(s) 128, 521
MnlI CCTC 5 cut(s) 51, 113, 160, 564, 935
Mox20I TGGCCA 1 cut(s) 89
Mph1103I ATGCAT 1 cut(s) 643
MroXI GAANNNNTTC 1 cut(s) 786
MscI TGGCCA 1 cut(s) 89
MseI TTAA 4 cut(s) 50, 266, 276, 441
MslI CAYNNNNRTG 3 cut(s) 236, 297, 389
Msp20I TGGCCA 1 cut(s) 89
MspA1I CMGCKG 1 cut(s) 836
MssI GTTTAAAC 1 cut(s) 51
MunI CAATTG 1 cut(s) 709
Mva1269I GAATGC 1 cut(s) 688
MwoI GCNNNNNNNGC 1 cut(s) 562
NdeII GATC 5 cut(s) 202, 334, 677, 724, 774
NlaIII CATG 2 cut(s) 540, 818
NmuCI GTSAC 2 cut(s) 306, 938
NsiI ATGCAT 1 cut(s) 643
NspI RCATGY 1 cut(s) 540
OliI CACNNNNGTG 1 cut(s) 389
PceI AGGCCT 1 cut(s) 147
PctI GAATGC 1 cut(s) 688
PdmI GAANNNNTTC 1 cut(s) 786
PfeI GAWTC 2 cut(s) 782, 869
PkrI GCNGC 2 cut(s) 765, 838
PleI GAGTC 2 cut(s) 125, 905
PmaCI CACGTG 1 cut(s) 387
PmeI GTTTAAAC 1 cut(s) 51
PmlI CACGTG 1 cut(s) 387
PpsI GAGTC 2 cut(s) 125, 905
Ppu21I YACGTR 2 cut(s) 387, 501
PspCI CACGTG 1 cut(s) 387
PstI CTGCAG 1 cut(s) 841
PvuII CAGCTG 1 cut(s) 836
RsaI GTAC 6 cut(s) 63, 116, 271, 503, 588, 610
RsaNI GTAC 6 cut(s) 62, 115, 270, 502, 587, 609
RseI CAYNNNNRTG 3 cut(s) 236, 297, 389
SaqAI TTAA 4 cut(s) 50, 266, 276, 441
SatI GCNGC 2 cut(s) 764, 837
Sau3AI GATC 5 cut(s) 202, 334, 677, 724, 774
SchI GAGTC 2 cut(s) 125, 905
SduI GDGCHC 1 cut(s) 174
SfaNI GCATC 2 cut(s) 365, 650
SfcI CTRYAG 1 cut(s) 837
SmiMI CAYNNNNRTG 3 cut(s) 236, 297, 389
SnaBI TACGTA 1 cut(s) 501
Sse9I AATT 4 cut(s) 99, 110, 438, 709
SseBI AGGCCT 1 cut(s) 147
SspMI CTAG 1 cut(s) 162
StuI AGGCCT 1 cut(s) 147
StyI CCWWGG 1 cut(s) 720
TaaI ACNGT 3 cut(s) 80, 212, 466
TaiI ACGT 2 cut(s) 389, 503
TaqI TCGA 2 cut(s) 331, 359
TasI AATT 4 cut(s) 99, 110, 438, 709
TatI WGTACW 3 cut(s) 114, 269, 586
TfiI GAWTC 2 cut(s) 782, 869
Tru1I TTAA 4 cut(s) 50, 266, 276, 441
Tru9I TTAA 4 cut(s) 50, 266, 276, 441
TscAI CASTG 2 cut(s) 24, 238
TseFI GTSAC 2 cut(s) 306, 938
TseI GCWGC 2 cut(s) 763, 836
Tsp45I GTSAC 2 cut(s) 306, 938
TspDTI ATGAA 6 cut(s) 27, 281, 339, 725, 795, 803
TspGWI ACGGA 1 cut(s) 775
TspRI CASTG 2 cut(s) 24, 238
XagI CCTNNNNNAGG 1 cut(s) 563
XbaI TCTAGA 1 cut(s) 161
XceI RCATGY 1 cut(s) 540
XmnI GAANNNNTTC 1 cut(s) 786
XspI CTAG 1 cut(s) 162
Zsp2I ATGCAT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.