Rh5AG003300

transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0006021
Physical Location & Seq
Forward (+)
259383 .. 262602
3220 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG003300.1

Sequence Viewer

Length: 702 bp
ATGCCAGTCTTTGCTATTGGGGTTGGTTTAAGTACATTTAATAAAGCGTCTGTTCATTACTATGTGCAGTCACACATTCAAATCAATGAATATCGAGATCGTGTTATTTTGCCGTCTGCATCGAAGAAGTATGGTAAGCCTATTACCACGTGTGTGAAGGTTTTAGATATGACTGGGTTGAAGCTTTCAGCACTAAACCAAATTAAGTTGTTGACAATTATATCCACAGTTGATGATTTGAACTACCCAGAGAAGACAAATACGTACTACATTGTAAATGTGCCTTACATATTCTCAGCCTGTTGGAAGGTTGTCAAGCCCCTTTTGCAGGAGAGGACAAGGAAAAAAGTACAAGTATTGTCTGGTTGTGGTACAGATGAGTTGTTGAAGATAATGGATTATGCATCTCTTCCCCATTTCTGTAAAAGGGAAGGTTCTGGATCATCTCGGCATTCACAGAATGGAACTGACAATTGCTTCTCCTTGGATGACCGTTTTCATCAACAGCTCTACAACTACATCAAGCAGCAATCTTTGATCAATGAATCCGTTCAACCAATCAAGCAGGGTTCTTTTCATGTGGACATACCTGAGCGAGCTGCAGAAGGAACACAGATAACAATAGAATCTGAGTTCAACAAGTTTGGTAACGGCAATGGGGTATCTGATTCTCTAAATGCCCTCAAAATCAATGGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

233

Amino Acids

26.16

Weight (kDa)

8.7

Isoelectric Point (pI)

32.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 43 - 140 9.2e-17 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 448
AcvI CACGTG 1 cut(s) 150
AfaI GTAC 4 cut(s) 34, 266, 351, 373
AfiI CCNNNNNNNGG 1 cut(s) 328
AflIII ACRYGT 1 cut(s) 149
AgsI TTSAA 6 cut(s) 80, 181, 241, 388, 554, 637
AleI CACNNNNGTG 1 cut(s) 152
AluBI AGCT 3 cut(s) 184, 508, 599
AluI AGCT 3 cut(s) 184, 508, 599
AlwI GGATC 1 cut(s) 448
ApeKI GCWGC 2 cut(s) 526, 599
Asp700I GAANNNNTTC 1 cut(s) 549
BbrPI CACGTG 1 cut(s) 150
BbsI GAAGAC 1 cut(s) 260
BbvI GCAGC 2 cut(s) 538, 586
BceAI ACGGC 2 cut(s) 97, 667
BclI TGATCA 1 cut(s) 537
BfmI CTRYAG 1 cut(s) 600
BisI GCNGC 2 cut(s) 527, 600
BlsI GCNGC 2 cut(s) 528, 601
BmrI ACTGGG 1 cut(s) 183
BmsI GCATC 2 cut(s) 128, 413
BmuI ACTGGG 1 cut(s) 183
BpiI GAAGAC 1 cut(s) 260
Bpu10I CCTNAGC 1 cut(s) 591
BsaAI YACGTR 2 cut(s) 150, 264
BsaJI CCNNGG 1 cut(s) 483
Bsc4I CCNNNNNNNGG 1 cut(s) 328
Bse1I ACTGG 2 cut(s) 5, 178
Bse3DI GCAATG 1 cut(s) 661
BseDI CCNNGG 1 cut(s) 483
BseGI GGATG 1 cut(s) 493
BseLI CCNNNNNNNGG 1 cut(s) 328
BseMI GCAATG 1 cut(s) 661
BseMII CTCAG 3 cut(s) 309, 582, 621
BseNI ACTGG 2 cut(s) 5, 178
BseXI GCAGC 2 cut(s) 538, 586
BsgI GTGCAG 1 cut(s) 86
BslI CCNNNNNNNGG 1 cut(s) 328
BsmI GAATGC 1 cut(s) 451
Bsp143I GATC 3 cut(s) 97, 440, 537
BspCNI CTCAG 3 cut(s) 308, 583, 622
BspMAI CTGCAG 1 cut(s) 604
BspPI GGATC 1 cut(s) 448
BsrDI GCAATG 1 cut(s) 661
BsrI ACTGG 2 cut(s) 5, 178
BssECI CCNNGG 1 cut(s) 483
BssMI GATC 3 cut(s) 97, 440, 537
BssT1I CCWWGG 1 cut(s) 483
Bst4CI ACNGT 2 cut(s) 229, 494
Bst6I CTCTTC 1 cut(s) 414
BstBAI YACGTR 2 cut(s) 150, 264
BstC8I GCNNGC 1 cut(s) 597
BstDEI CTNAG 3 cut(s) 295, 591, 630
BstENI CCTNNNNNAGG 1 cut(s) 326
BstF5I GGATG 1 cut(s) 493
BstKTI GATC 3 cut(s) 100, 443, 540
BstMBI GATC 3 cut(s) 97, 440, 537
BstMWI GCNNNNNNNGC 1 cut(s) 325
BstSFI CTRYAG 1 cut(s) 600
BstSNI TACGTA 1 cut(s) 264
BstV1I GCAGC 2 cut(s) 538, 586
BstV2I GAAGAC 1 cut(s) 260
BtsCI GGATG 1 cut(s) 493
Cac8I GCNNGC 1 cut(s) 597
CseI GACGC 1 cut(s) 36
Csp6I GTAC 4 cut(s) 33, 265, 350, 372
CviAII CATG 1 cut(s) 578
CviJI RGCY 6 cut(s) 139, 184, 299, 319, 508, 599
CviKI_1 RGCY 6 cut(s) 139, 184, 299, 319, 508, 599
CviQI GTAC 4 cut(s) 33, 265, 350, 372
DdeI CTNAG 3 cut(s) 295, 591, 630
DpnI GATC 3 cut(s) 99, 442, 539
DpnII GATC 3 cut(s) 97, 440, 537
Eam1104I CTCTTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 414
Eco105I TACGTA 1 cut(s) 264
Eco130I CCWWGG 1 cut(s) 483
Eco72I CACGTG 1 cut(s) 150
EcoNI CCTNNNNNAGG 1 cut(s) 326
EcoT14I CCWWGG 1 cut(s) 483
EcoT22I ATGCAT 1 cut(s) 406
ErhI CCWWGG 1 cut(s) 483
FaeI CATG 1 cut(s) 581
FaiI YATR 8 cut(s) 63, 132, 170, 221, 290, 402, 579, 587
FatI CATG 1 cut(s) 577
FbaI TGATCA 1 cut(s) 537
Fnu4HI GCNGC 2 cut(s) 527, 600
FokI GGATG 1 cut(s) 500
Fsp4HI GCNGC 2 cut(s) 527, 600
GluI GCNGC 2 cut(s) 527, 600
HgaI GACGC 1 cut(s) 36
Hin1II CATG 1 cut(s) 581
HincII GTYRAC 1 cut(s) 213
HindII GTYRAC 1 cut(s) 213
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 3 cut(s) 545, 626, 668
Hpy166II GTNNAC 2 cut(s) 213, 583
Hpy188I TCNGA 2 cut(s) 631, 667
Hpy188III TCNNGA 2 cut(s) 95, 438
Hpy8I GTNNAC 2 cut(s) 213, 583
HpyAV CCTTC 4 cut(s) 151, 301, 425, 599
HpyCH4III ACNGT 2 cut(s) 229, 494
HpyCH4IV ACGT 2 cut(s) 149, 263
HpyCH4V TGCA 5 cut(s) 67, 119, 328, 404, 602
HpyF10VI GCNNNNNNNGC 1 cut(s) 325
HpyF3I CTNAG 3 cut(s) 295, 591, 630
HpySE526I ACGT 2 cut(s) 149, 263
Hsp92II CATG 1 cut(s) 581
Ksp22I TGATCA 1 cut(s) 537
Kzo9I GATC 3 cut(s) 97, 440, 537
LpnPI CCDG 9 cut(s) 18, 159, 261, 313, 314, 348, 423, 551, 603
Lsp1109I GCAGC 2 cut(s) 538, 586
LweI GCATC 2 cut(s) 128, 413
MaeII ACGT 2 cut(s) 149, 263
MaeIII GTNAC 3 cut(s) 69, 647, 695
MalI GATC 3 cut(s) 99, 442, 539
MboI GATC 3 cut(s) 97, 440, 537
MboII GAAGA 4 cut(s) 136, 265, 400, 401
MfeI CAATTG 1 cut(s) 472
MluCI AATT 3 cut(s) 201, 216, 472
MmeI TCCRAC 1 cut(s) 284
MnlI CCTC 2 cut(s) 327, 692
Mph1103I ATGCAT 1 cut(s) 406
MroXI GAANNNNTTC 1 cut(s) 549
MseI TTAA 3 cut(s) 29, 39, 204
MslI CAYNNNNRTG 2 cut(s) 60, 152
MunI CAATTG 1 cut(s) 472
Mva1269I GAATGC 1 cut(s) 451
MwoI GCNNNNNNNGC 1 cut(s) 325
NdeII GATC 3 cut(s) 97, 440, 537
NlaIII CATG 1 cut(s) 581
NmeAIII GCCGAG 1 cut(s) 427
NmuCI GTSAC 2 cut(s) 69, 695
NsiI ATGCAT 1 cut(s) 406
OliI CACNNNNGTG 1 cut(s) 152
PctI GAATGC 1 cut(s) 451
PdmI GAANNNNTTC 1 cut(s) 549
PfeI GAWTC 3 cut(s) 545, 626, 668
PkrI GCNGC 2 cut(s) 528, 601
PmaCI CACGTG 1 cut(s) 150
PmlI CACGTG 1 cut(s) 150
Ppu21I YACGTR 2 cut(s) 150, 264
PspCI CACGTG 1 cut(s) 150
PstI CTGCAG 1 cut(s) 604
RsaI GTAC 4 cut(s) 34, 266, 351, 373
RsaNI GTAC 4 cut(s) 33, 265, 350, 372
RseI CAYNNNNRTG 2 cut(s) 60, 152
SaqAI TTAA 3 cut(s) 29, 39, 204
SatI GCNGC 2 cut(s) 527, 600
Sau3AI GATC 3 cut(s) 97, 440, 537
SetI ASST 9 cut(s) 152, 162, 186, 266, 312, 436, 510, 592, 601
SfaNI GCATC 2 cut(s) 128, 413
SfcI CTRYAG 1 cut(s) 600
SmiMI CAYNNNNRTG 2 cut(s) 60, 152
SnaBI TACGTA 1 cut(s) 264
Sse9I AATT 3 cut(s) 201, 216, 472
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 2 cut(s) 229, 494
TaiI ACGT 2 cut(s) 152, 266
TaqI TCGA 2 cut(s) 94, 122
TasI AATT 3 cut(s) 201, 216, 472
TatI WGTACW 2 cut(s) 32, 349
TfiI GAWTC 3 cut(s) 545, 626, 668
Tru1I TTAA 3 cut(s) 29, 39, 204
Tru9I TTAA 3 cut(s) 29, 39, 204
TseFI GTSAC 2 cut(s) 69, 695
TseI GCWGC 2 cut(s) 526, 599
Tsp45I GTSAC 2 cut(s) 69, 695
TspDTI ATGAA 5 cut(s) 44, 102, 488, 558, 566
TspGWI ACGGA 1 cut(s) 538
XagI CCTNNNNNAGG 1 cut(s) 326
XmnI GAANNNNTTC 1 cut(s) 549
Zsp2I ATGCAT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.