FvH4_4g17420

transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006021
Physical Location & Seq
Reverse (-)
21362615 .. 21364148
1534 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g17420.t1

Sequence Viewer

Length: 429 bp
ATGTTGATAGATTGTTTAAACTGGAGGGTGCAAAATGAGATTGACAATATATTGGCTAAACCCATACTTCCAATTGAATTGTACAGAGGAGTGCGAGAGTCACAACTCATAGGCCTTTCAGGTTACTCTAGAGAGGGTATGCCAGTCTTTGCTATTGGGGTTGGGTTAAGTACATTTGATAAAGCGTCTGTTCACTACTATGTGCAGTCACACATTCAAATCAATGAATATCGAGATCATGTTATTTTGAAGTATGGTAAGCCTATTACCACGTGTGTGAAAGTTTTAGATATGACTGGCTTGAAGCTTTCAGCACTAAGCCACATTAAGTTGTTGACAATTATATCAACAGTTGATGATTTGAATTACCCAGAGAAGACAAGTATGTACTACATTGTAAATGCGCCTTACATATTCTCAGCTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

143

Amino Acids

16.09

Weight (kDa)

6.89

Isoelectric Point (pI)

42.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 33 - 141 1.9e-08 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcvI CACGTG 1 cut(s) 273
AfaI GTAC 3 cut(s) 83, 172, 389
AflIII ACRYGT 1 cut(s) 272
AgsI TTSAA 5 cut(s) 77, 218, 250, 304, 364
AleI CACNNNNGTG 1 cut(s) 275
AluBI AGCT 2 cut(s) 307, 422
AluI AGCT 2 cut(s) 307, 422
AoxI GGCC 1 cut(s) 112
AspLEI GCGC 1 cut(s) 406
BbrPI CACGTG 1 cut(s) 273
BbsI GAAGAC 1 cut(s) 383
BfaI CTAG 1 cut(s) 129
BpiI GAAGAC 1 cut(s) 383
BpmI CTGGAG 1 cut(s) 43
BsaAI YACGTR 1 cut(s) 273
Bse1I ACTGG 3 cut(s) 26, 143, 301
BseNI ACTGG 3 cut(s) 26, 143, 301
BseRI GAGGAG 1 cut(s) 102
BsgI GTGCAG 1 cut(s) 224
BshFI GGCC 1 cut(s) 114
BsnI GGCC 1 cut(s) 114
Bsp1407I TGTACA 1 cut(s) 81
Bsp143I GATC 1 cut(s) 235
BspANI GGCC 1 cut(s) 114
BsrGI TGTACA 1 cut(s) 81
BsrI ACTGG 3 cut(s) 26, 143, 301
BssMI GATC 1 cut(s) 235
Bst4CI ACNGT 1 cut(s) 352
BstAUI TGTACA 1 cut(s) 81
BstBAI YACGTR 1 cut(s) 273
BstDEI CTNAG 2 cut(s) 317, 418
BstHHI GCGC 1 cut(s) 406
BstKTI GATC 1 cut(s) 238
BstMBI GATC 1 cut(s) 235
BstV2I GAAGAC 1 cut(s) 383
BsuRI GGCC 1 cut(s) 114
CfoI GCGC 1 cut(s) 406
CseI GACGC 1 cut(s) 174
Csp6I GTAC 3 cut(s) 82, 171, 388
CviAII CATG 1 cut(s) 239
CviJI RGCY 7 cut(s) 56, 114, 262, 300, 307, 321, 422
CviKI_1 RGCY 7 cut(s) 56, 114, 262, 300, 307, 321, 422
CviQI GTAC 3 cut(s) 82, 171, 388
DdeI CTNAG 2 cut(s) 317, 418
DpnI GATC 1 cut(s) 237
DpnII GATC 1 cut(s) 235
DraI TTTAAA 1 cut(s) 18
Eco147I AGGCCT 1 cut(s) 114
Eco72I CACGTG 1 cut(s) 273
FaeI CATG 1 cut(s) 242
FatI CATG 1 cut(s) 238
FspBI CTAG 1 cut(s) 129
GlaI GCGC 1 cut(s) 405
GsuI CTGGAG 1 cut(s) 43
HaeIII GGCC 1 cut(s) 114
HgaI GACGC 1 cut(s) 174
HhaI GCGC 1 cut(s) 406
Hin1II CATG 1 cut(s) 242
Hin6I GCGC 1 cut(s) 404
HinP1I GCGC 1 cut(s) 404
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HindIII AAGCTT 1 cut(s) 305
HinfI GANTC 1 cut(s) 98
Hpy166II GTNNAC 2 cut(s) 193, 336
Hpy188III TCNNGA 2 cut(s) 129, 233
Hpy8I GTNNAC 2 cut(s) 193, 336
HpyCH4III ACNGT 1 cut(s) 352
HpyCH4IV ACGT 1 cut(s) 272
HpyCH4V TGCA 2 cut(s) 31, 205
HpyF3I CTNAG 2 cut(s) 317, 418
HpySE526I ACGT 1 cut(s) 272
Hsp92II CATG 1 cut(s) 242
HspAI GCGC 1 cut(s) 404
Kzo9I GATC 1 cut(s) 235
LpnPI CCDG 5 cut(s) 7, 105, 156, 282, 384
MaeI CTAG 1 cut(s) 129
MaeII ACGT 1 cut(s) 272
MaeIII GTNAC 3 cut(s) 99, 122, 207
MalI GATC 1 cut(s) 237
MboI GATC 1 cut(s) 235
MboII GAAGA 1 cut(s) 388
MfeI CAATTG 1 cut(s) 72
MluCI AATT 4 cut(s) 72, 77, 339, 364
MlyI GAGTC 1 cut(s) 107
MnlI CCTC 3 cut(s) 18, 80, 127
MseI TTAA 3 cut(s) 17, 167, 327
MslI CAYNNNNRTG 2 cut(s) 198, 275
MssI GTTTAAAC 1 cut(s) 18
MunI CAATTG 1 cut(s) 72
NdeII GATC 1 cut(s) 235
NlaIII CATG 1 cut(s) 242
NmuCI GTSAC 2 cut(s) 99, 207
OliI CACNNNNGTG 1 cut(s) 275
PceI AGGCCT 1 cut(s) 114
PleI GAGTC 1 cut(s) 106
PmaCI CACGTG 1 cut(s) 273
PmeI GTTTAAAC 1 cut(s) 18
PmlI CACGTG 1 cut(s) 273
PpsI GAGTC 1 cut(s) 106
Ppu21I YACGTR 1 cut(s) 273
PspCI CACGTG 1 cut(s) 273
RsaI GTAC 3 cut(s) 83, 172, 389
RsaNI GTAC 3 cut(s) 82, 171, 388
RseI CAYNNNNRTG 2 cut(s) 198, 275
SaqAI TTAA 3 cut(s) 17, 167, 327
Sau3AI GATC 1 cut(s) 235
SchI GAGTC 1 cut(s) 107
SetI ASST 4 cut(s) 124, 275, 309, 424
SmiMI CAYNNNNRTG 2 cut(s) 198, 275
Sse9I AATT 4 cut(s) 72, 77, 339, 364
SseBI AGGCCT 1 cut(s) 114
SspMI CTAG 1 cut(s) 129
StuI AGGCCT 1 cut(s) 114
TaaI ACNGT 1 cut(s) 352
TaiI ACGT 1 cut(s) 275
TaqI TCGA 1 cut(s) 232
TasI AATT 4 cut(s) 72, 77, 339, 364
TatI WGTACW 3 cut(s) 81, 170, 387
Tru1I TTAA 3 cut(s) 17, 167, 327
Tru9I TTAA 3 cut(s) 17, 167, 327
TseFI GTSAC 2 cut(s) 99, 207
Tsp45I GTSAC 2 cut(s) 99, 207
TspDTI ATGAA 1 cut(s) 240
XbaI TCTAGA 1 cut(s) 128
XspI CTAG 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.