RLG00000030864

transfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0006021
Physical Location & Seq
Forward (+)
290156 .. 293663
3508 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030864

Sequence Viewer

Length: 846 bp
ATGTTGATGGATTGTTTAAACTGGAGGGTACAAAATGAGATTGACAGTATATTGGCCAAACCCATAATTCCAACTGAATTGTACAGAGGAGTGCGAGACTCACAGCTCATAGGCCTTTCAGGTTACTCTAGAGAGGGTATGCCAGTCTTTGCTATTGGGGTTGGGTTAAGTACATTTAATAGAGCGTCTGTTCATTACTATGTGCAGTCACACATTCAAATCAATGAATATCGAGATCGTGTTATTTTGCCATCTGCATCGAAGAAGTATGGTAAGCCTATTACCACGTGTGTGAAGGTTTTAGATATGACTGGGTTGAAGCTTTCAGCACTAAACCAAATTAAGTTGTTGACAATTATATCAACAGTTGATGATTTGAACTACCCAGAGAAGACAAATACGTACTACATTGTAAATGCGCCTTACATATTCTCAGCCTGTTGGAAGGTTGTCAAGCCCCTTTTGCAGGAGAGGACAAGAAAAAAGGTACAAGTATTGTCTGGTTGTGGTACAGATGAGTTGTTGAAGATAATGGATTATGCATCTCTTCCCCATTTCTGTAAAAGGGAAGGTTCTGGATCATCTCGGCATTCACAGAATGGAACTGACAATTGCTTCTCCTTGGATCACCATTTTCATCAACAGCTCTACAACTACATCAAGCAGCAATCTTTGATCAATGAATCCGTTCAACCAATCAAGCAGGGTTCTTTTCATCTGGACATACCTGAGCCAGCTGCAGAAGGAACACAGATTGCCAAAACAATAGAATCTGAGTTCAACAAGTTTGGTAATGGCAATGGGGTATCTGATTCTCTAAATGGCCTCAAAATCAATGGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

282

Amino Acids

31.54

Weight (kDa)

8.42

Isoelectric Point (pI)

32.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 34 - 186 2.9e-18 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 586, 633
AcoI YGGCCR 1 cut(s) 54
AcvI CACGTG 1 cut(s) 288
AfaI GTAC 6 cut(s) 30, 83, 172, 404, 489, 511
AfiI CCNNNNNNNGG 1 cut(s) 466
AflIII ACRYGT 1 cut(s) 287
AgsI TTSAA 6 cut(s) 218, 319, 379, 526, 692, 781
AleI CACNNNNGTG 1 cut(s) 290
AluBI AGCT 4 cut(s) 106, 322, 646, 737
AluI AGCT 4 cut(s) 106, 322, 646, 737
Alw26I GTCTC 1 cut(s) 90
AlwI GGATC 2 cut(s) 586, 633
AoxI GGCC 3 cut(s) 54, 112, 823
ApeKI GCWGC 2 cut(s) 664, 737
Asp700I GAANNNNTTC 1 cut(s) 687
AspLEI GCGC 1 cut(s) 421
AsuHPI GGTGA 1 cut(s) 620
BalI TGGCCA 1 cut(s) 56
BbrPI CACGTG 1 cut(s) 288
BbsI GAAGAC 1 cut(s) 398
BbvI GCAGC 2 cut(s) 676, 724
BccI CCATC 1 cut(s) 259
BclI TGATCA 1 cut(s) 675
BcoDI GTCTC 1 cut(s) 90
BfaI CTAG 1 cut(s) 129
BfmI CTRYAG 1 cut(s) 738
BisI GCNGC 2 cut(s) 665, 738
BlsI GCNGC 2 cut(s) 666, 739
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 2 cut(s) 266, 551
BmuI ACTGGG 1 cut(s) 321
BpiI GAAGAC 1 cut(s) 398
BpmI CTGGAG 1 cut(s) 43
Bpu10I CCTNAGC 1 cut(s) 729
BsaAI YACGTR 2 cut(s) 288, 402
BsaJI CCNNGG 1 cut(s) 621
Bsc4I CCNNNNNNNGG 1 cut(s) 466
Bse1I ACTGG 3 cut(s) 26, 143, 316
Bse3DI GCAATG 1 cut(s) 805
BseDI CCNNGG 1 cut(s) 621
BseLI CCNNNNNNNGG 1 cut(s) 466
BseMI GCAATG 1 cut(s) 805
BseMII CTCAG 3 cut(s) 447, 720, 765
BseNI ACTGG 3 cut(s) 26, 143, 316
BseRI GAGGAG 1 cut(s) 102
BseXI GCAGC 2 cut(s) 676, 724
BsgI GTGCAG 1 cut(s) 224
BshFI GGCC 3 cut(s) 56, 114, 825
BslI CCNNNNNNNGG 1 cut(s) 466
BsmAI GTCTC 1 cut(s) 90
BsmI GAATGC 1 cut(s) 589
BsnI GGCC 3 cut(s) 56, 114, 825
Bsp1407I TGTACA 1 cut(s) 81
Bsp143I GATC 4 cut(s) 235, 578, 625, 675
BspANI GGCC 3 cut(s) 56, 114, 825
BspCNI CTCAG 3 cut(s) 446, 721, 766
BspMAI CTGCAG 1 cut(s) 742
BspPI GGATC 2 cut(s) 586, 633
BsrDI GCAATG 1 cut(s) 805
BsrGI TGTACA 1 cut(s) 81
BsrI ACTGG 3 cut(s) 26, 143, 316
BssECI CCNNGG 1 cut(s) 621
BssMI GATC 4 cut(s) 235, 578, 625, 675
BssT1I CCWWGG 1 cut(s) 621
Bst4CI ACNGT 2 cut(s) 47, 367
Bst6I CTCTTC 1 cut(s) 552
BstAUI TGTACA 1 cut(s) 81
BstBAI YACGTR 2 cut(s) 288, 402
BstC8I GCNNGC 1 cut(s) 735
BstDEI CTNAG 3 cut(s) 433, 729, 774
BstENI CCTNNNNNAGG 1 cut(s) 464
BstHHI GCGC 1 cut(s) 421
BstKTI GATC 4 cut(s) 238, 581, 628, 678
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 4 cut(s) 235, 578, 625, 675
BstMWI GCNNNNNNNGC 1 cut(s) 463
BstSFI CTRYAG 1 cut(s) 738
BstSNI TACGTA 1 cut(s) 402
BstV1I GCAGC 2 cut(s) 676, 724
BstV2I GAAGAC 1 cut(s) 398
BsuRI GGCC 3 cut(s) 56, 114, 825
Cac8I GCNNGC 1 cut(s) 735
CfoI GCGC 1 cut(s) 421
CseI GACGC 1 cut(s) 174
Csp6I GTAC 6 cut(s) 29, 82, 171, 403, 488, 510
CviQI GTAC 6 cut(s) 29, 82, 171, 403, 488, 510
DdeI CTNAG 3 cut(s) 433, 729, 774
DpnI GATC 4 cut(s) 237, 580, 627, 677
DpnII GATC 4 cut(s) 235, 578, 625, 675
DraI TTTAAA 1 cut(s) 18
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
Eco105I TACGTA 1 cut(s) 402
Eco130I CCWWGG 1 cut(s) 621
Eco147I AGGCCT 1 cut(s) 114
Eco72I CACGTG 1 cut(s) 288
EcoNI CCTNNNNNAGG 1 cut(s) 464
EcoT14I CCWWGG 1 cut(s) 621
EcoT22I ATGCAT 1 cut(s) 544
ErhI CCWWGG 1 cut(s) 621
FbaI TGATCA 1 cut(s) 675
Fnu4HI GCNGC 2 cut(s) 665, 738
Fsp4HI GCNGC 2 cut(s) 665, 738
FspBI CTAG 1 cut(s) 129
GlaI GCGC 1 cut(s) 420
GluI GCNGC 2 cut(s) 665, 738
GsuI CTGGAG 1 cut(s) 43
HaeIII GGCC 3 cut(s) 56, 114, 825
HgaI GACGC 1 cut(s) 174
HhaI GCGC 1 cut(s) 421
Hin6I GCGC 1 cut(s) 419
HinP1I GCGC 1 cut(s) 419
HincII GTYRAC 1 cut(s) 351
HindII GTYRAC 1 cut(s) 351
HindIII AAGCTT 1 cut(s) 320
HinfI GANTC 4 cut(s) 98, 683, 770, 812
HphI GGTGA 1 cut(s) 620
Hpy166II GTNNAC 1 cut(s) 351
Hpy188I TCNGA 2 cut(s) 775, 811
Hpy188III TCNNGA 4 cut(s) 129, 233, 576, 719
Hpy8I GTNNAC 1 cut(s) 351
HpyAV CCTTC 4 cut(s) 289, 439, 563, 737
HpyCH4III ACNGT 2 cut(s) 47, 367
HpyCH4IV ACGT 2 cut(s) 287, 401
HpyCH4V TGCA 5 cut(s) 205, 257, 466, 542, 740
HpyF10VI GCNNNNNNNGC 1 cut(s) 463
HpyF3I CTNAG 3 cut(s) 433, 729, 774
HpySE526I ACGT 2 cut(s) 287, 401
HspAI GCGC 1 cut(s) 419
Ksp22I TGATCA 1 cut(s) 675
Kzo9I GATC 4 cut(s) 235, 578, 625, 675
Lsp1109I GCAGC 2 cut(s) 676, 724
LweI GCATC 2 cut(s) 266, 551
MaeI CTAG 1 cut(s) 129
MaeII ACGT 2 cut(s) 287, 401
MaeIII GTNAC 3 cut(s) 122, 207, 839
MalI GATC 4 cut(s) 237, 580, 627, 677
MboI GATC 4 cut(s) 235, 578, 625, 675
MboII GAAGA 4 cut(s) 274, 403, 538, 539
MfeI CAATTG 1 cut(s) 610
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 5 cut(s) 66, 77, 339, 354, 610
MluNI TGGCCA 1 cut(s) 56
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 2 cut(s) 95, 422
MnlI CCTC 5 cut(s) 18, 80, 127, 465, 836
Mox20I TGGCCA 1 cut(s) 56
Mph1103I ATGCAT 1 cut(s) 544
MroXI GAANNNNTTC 1 cut(s) 687
MscI TGGCCA 1 cut(s) 56
MseI TTAA 4 cut(s) 17, 167, 177, 342
MslI CAYNNNNRTG 2 cut(s) 198, 290
Msp20I TGGCCA 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 737
MssI GTTTAAAC 1 cut(s) 18
MunI CAATTG 1 cut(s) 610
Mva1269I GAATGC 1 cut(s) 589
MwoI GCNNNNNNNGC 1 cut(s) 463
NdeII GATC 4 cut(s) 235, 578, 625, 675
NmeAIII GCCGAG 1 cut(s) 565
NmuCI GTSAC 2 cut(s) 207, 839
NsiI ATGCAT 1 cut(s) 544
OliI CACNNNNGTG 1 cut(s) 290
PceI AGGCCT 1 cut(s) 114
PctI GAATGC 1 cut(s) 589
PdmI GAANNNNTTC 1 cut(s) 687
PfeI GAWTC 3 cut(s) 683, 770, 812
PkrI GCNGC 2 cut(s) 666, 739
PleI GAGTC 1 cut(s) 92
PmaCI CACGTG 1 cut(s) 288
PmeI GTTTAAAC 1 cut(s) 18
PmlI CACGTG 1 cut(s) 288
PpsI GAGTC 1 cut(s) 92
Ppu21I YACGTR 2 cut(s) 288, 402
PspCI CACGTG 1 cut(s) 288
PstI CTGCAG 1 cut(s) 742
PvuII CAGCTG 1 cut(s) 737
RsaI GTAC 6 cut(s) 30, 83, 172, 404, 489, 511
RsaNI GTAC 6 cut(s) 29, 82, 171, 403, 488, 510
RseI CAYNNNNRTG 2 cut(s) 198, 290
SaqAI TTAA 4 cut(s) 17, 167, 177, 342
SatI GCNGC 2 cut(s) 665, 738
Sau3AI GATC 4 cut(s) 235, 578, 625, 675
SchI GAGTC 1 cut(s) 92
SfaNI GCATC 2 cut(s) 266, 551
SfcI CTRYAG 1 cut(s) 738
SmiMI CAYNNNNRTG 2 cut(s) 198, 290
SnaBI TACGTA 1 cut(s) 402
Sse9I AATT 5 cut(s) 66, 77, 339, 354, 610
SseBI AGGCCT 1 cut(s) 114
SspMI CTAG 1 cut(s) 129
StuI AGGCCT 1 cut(s) 114
StyI CCWWGG 1 cut(s) 621
TaaI ACNGT 2 cut(s) 47, 367
TaiI ACGT 2 cut(s) 290, 404
TaqI TCGA 2 cut(s) 232, 260
TasI AATT 5 cut(s) 66, 77, 339, 354, 610
TatI WGTACW 2 cut(s) 81, 170
TfiI GAWTC 3 cut(s) 683, 770, 812
Tru1I TTAA 4 cut(s) 17, 167, 177, 342
Tru9I TTAA 4 cut(s) 17, 167, 177, 342
TseFI GTSAC 2 cut(s) 207, 839
TseI GCWGC 2 cut(s) 664, 737
Tsp45I GTSAC 2 cut(s) 207, 839
TspDTI ATGAA 5 cut(s) 182, 240, 626, 696, 704
TspGWI ACGGA 1 cut(s) 676
XagI CCTNNNNNAGG 1 cut(s) 464
XbaI TCTAGA 1 cut(s) 128
XmnI GAANNNNTTC 1 cut(s) 687
XspI CTAG 1 cut(s) 129
Zsp2I ATGCAT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.