AT5G52380

Zinc knuckle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008202
Physical Location & Seq
Reverse (-)
21261353 .. 21263151
1799 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G52380.1

Sequence Viewer

Length: 807 bp
ATGGTGAACCAAAGGAGAAGACTTGCGCAGAAGCGATACAAGGAAGCTAATCCAGAGCTGTTTCCAAAAGCAGAACCAACGCCACCAAAGGACCCTAATAAGAAGAAGAAGAAGAAAAGCTTGTTCAAGAAAAAGAAACCTGGATCTTCCACTGATAGACCTCAGAGAACAGGCTCTTCCACAAGACATCCTCTTCGAGTTCCTGGTATGAAACCTGGAGAAGGCTGTTTTATATGTCATTCCAAAACCCATATTGCTAAGCTTTGTCCTGAAAAATCTGAGTGGGAGAGAAACAAGATATGCTTGCAATGTAGGAGGAGAGGGCATAGTCTTAAAAACTGCCCTGAAAAGAACAATGAGAGCTCCGAGAAGAAGCTCTGTTACAATTGTGGAGATACTGGTCATTCACTTTCCCATTGTCCTTACCCTATGGAAGATGGAGGGACCAAATTTGCAAGTTGTTTCATATGCAAGGGACAAGGGCACATAAGCAAGAACTGTCCCGAAAACAAGCATGGAATCTACCCAATGGGCGGGTGTTGTAAAGTGTGCGGGAGTGTGGCGCATCTCGTCAAAGACTGTCCTGATAAATTCAACCAAGAGTCAGCCCAACCAAAGAAGACTTCAAGATTTGATGCTACACCAAGAGGAAAGCTCACTAAGTTTAGTGGGGACGATCTTGAAGACGATTTCACTGAAGAGCCCAAAAGCAGCAAGAAGATCAATACCTCTGATGATTCAGCTCAGAATAGTGTCGAGGTGAAGAAAAAGAAACAAGGTCCAAAGATTGTCAATTTCGTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF zf-CCHC zf-CCHC_4

Protein Analysis

268

Amino Acids

30.04

Weight (kDa)

9.57

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 27
AciI CCGC 2 cut(s) 534, 552
AclWI GGATC 1 cut(s) 151
AcsI RAATTY 2 cut(s) 449, 590
AcuI CTGAAG 1 cut(s) 717
AfiI CCNNNNNNNGG 2 cut(s) 221, 533
AgsI TTSAA 4 cut(s) 127, 595, 627, 683
AjnI CCWGG 3 cut(s) 139, 202, 214
AjuI GAANNNNNNNTTGG 2 cut(s) 607, 639
AluBI AGCT 8 cut(s) 47, 58, 120, 262, 363, 376, 655, 743
AluI AGCT 8 cut(s) 47, 58, 120, 262, 363, 376, 655, 743
Alw21I GWGCWC 1 cut(s) 365
AlwI GGATC 1 cut(s) 151
ApeKI GCWGC 1 cut(s) 711
ApoI RAATTY 2 cut(s) 449, 590
ArsI GACNNNNNNTTYG 2 cut(s) 177, 209
AspLEI GCGC 2 cut(s) 28, 565
AspS9I GGNCC 3 cut(s) 91, 444, 779
AsuHPI GGTGA 2 cut(s) 16, 772
AvaII GGWCC 3 cut(s) 91, 444, 779
BaeGI GKGCMC 1 cut(s) 486
BanII GRGCYC 2 cut(s) 365, 705
BarI GAAGNNNNNNTAC 2 cut(s) 365, 397
BbsI GAAGAC 3 cut(s) 25, 626, 690
Bbv12I GWGCWC 1 cut(s) 365
BbvI GCAGC 1 cut(s) 723
BccI CCATC 1 cut(s) 431
BciT130I CCWGG 3 cut(s) 141, 204, 216
BisI GCNGC 1 cut(s) 712
BlpI GCTNAGC 1 cut(s) 258
BlsI GCNGC 1 cut(s) 713
Bme1390I CCNGG 3 cut(s) 141, 204, 216
Bme18I GGWCC 3 cut(s) 91, 444, 779
BmgT120I GGNCC 3 cut(s) 91, 444, 779
BmiI GGNNCC 2 cut(s) 93, 445
BmrFI CCNGG 3 cut(s) 141, 204, 216
BmsI GCATC 2 cut(s) 574, 625
BpiI GAAGAC 3 cut(s) 25, 626, 690
BplI GAGNNNNNCTC 2 cut(s) 639, 671
BpmI CTGGAG 1 cut(s) 237
Bpu1102I GCTNAGC 1 cut(s) 258
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 533
Bse1I ACTGG 1 cut(s) 403
Bse3DI GCAATG 1 cut(s) 314
BseBI CCWGG 3 cut(s) 141, 204, 216
BseGI GGATG 1 cut(s) 187
BseLI CCNNNNNNNGG 2 cut(s) 221, 533
BseMI GCAATG 1 cut(s) 314
BseMII CTCAG 3 cut(s) 176, 270, 758
BseNI ACTGG 1 cut(s) 403
BseRI GAGGAG 1 cut(s) 331
BseSI GKGCMC 1 cut(s) 486
BseXI GCAGC 1 cut(s) 723
BsiHKAI GWGCWC 1 cut(s) 365
BslFI GGGAC 4 cut(s) 457, 486, 489, 686
BslI CCNNNNNNNGG 2 cut(s) 221, 533
BsmFI GGGAC 4 cut(s) 457, 486, 489, 686
Bsp1286I GDGCHC 3 cut(s) 365, 486, 705
Bsp143I GATC 3 cut(s) 143, 676, 720
Bsp1720I GCTNAGC 1 cut(s) 258
BspACI CCGC 2 cut(s) 534, 552
BspCNI CTCAG 3 cut(s) 175, 271, 757
BspLI GGNNCC 2 cut(s) 93, 445
BspPI GGATC 1 cut(s) 151
BspQI GCTCTTC 2 cut(s) 181, 693
BsrDI GCAATG 1 cut(s) 314
BsrI ACTGG 1 cut(s) 403
BssMI GATC 3 cut(s) 143, 676, 720
Bst2UI CCWGG 3 cut(s) 141, 204, 216
Bst4CI ACNGT 2 cut(s) 500, 581
Bst6I CTCTTC 3 cut(s) 181, 198, 693
BstC8I GCNNGC 1 cut(s) 305
BstDEI CTNAG 5 cut(s) 162, 258, 279, 660, 744
BstENI CCTNNNNNAGG 1 cut(s) 219
BstF5I GGATG 1 cut(s) 187
BstHHI GCGC 2 cut(s) 28, 565
BstKTI GATC 3 cut(s) 146, 679, 723
BstMBI GATC 3 cut(s) 143, 676, 720
BstNI CCWGG 3 cut(s) 141, 204, 216
BstSCI CCNGG 3 cut(s) 139, 202, 214
BstSLI GKGCMC 1 cut(s) 486
BstV1I GCAGC 1 cut(s) 723
BstV2I GAAGAC 3 cut(s) 25, 626, 690
BstX2I RGATCY 1 cut(s) 143
BstYI RGATCY 1 cut(s) 143
BtsCI GGATG 1 cut(s) 187
BtsIMutI CAGTG 2 cut(s) 150, 693
Cac8I GCNNGC 1 cut(s) 305
CfoI GCGC 2 cut(s) 28, 565
Cfr13I GGNCC 3 cut(s) 91, 444, 779
CviAII CATG 1 cut(s) 515
DdeI CTNAG 5 cut(s) 162, 258, 279, 660, 744
DpnI GATC 3 cut(s) 145, 678, 722
DpnII GATC 3 cut(s) 143, 676, 720
Eam1104I CTCTTC 3 cut(s) 181, 198, 693
EarI CTCTTC 3 cut(s) 181, 198, 693
Ecl136II GAGCTC 1 cut(s) 363
Eco24I GRGCYC 2 cut(s) 365, 705
Eco47I GGWCC 3 cut(s) 91, 444, 779
Eco53kI GAGCTC 1 cut(s) 363
Eco57I CTGAAG 1 cut(s) 717
EcoICRI GAGCTC 1 cut(s) 363
EcoNI CCTNNNNNAGG 1 cut(s) 219
EcoO109I RGGNCCY 1 cut(s) 91
EcoRII CCWGG 3 cut(s) 139, 202, 214
EcoT38I GRGCYC 2 cut(s) 365, 705
FaeI CATG 1 cut(s) 518
FalI AAGNNNNNCTT 4 cut(s) 104, 136, 287, 319
FaqI GGGAC 4 cut(s) 457, 486, 489, 686
FatI CATG 1 cut(s) 514
FauI CCCGC 2 cut(s) 527, 545
FauNDI CATATG 1 cut(s) 467
Fnu4HI GCNGC 1 cut(s) 712
FokI GGATG 1 cut(s) 174
FriOI GRGCYC 2 cut(s) 365, 705
Fsp4HI GCNGC 1 cut(s) 712
FspI TGCGCA 1 cut(s) 27
GlaI GCGC 2 cut(s) 27, 564
GluI GCNGC 1 cut(s) 712
GsuI CTGGAG 1 cut(s) 237
HhaI GCGC 2 cut(s) 28, 565
Hin1II CATG 1 cut(s) 518
Hin6I GCGC 2 cut(s) 26, 563
HinP1I GCGC 2 cut(s) 26, 563
HindIII AAGCTT 2 cut(s) 118, 260
HinfI GANTC 3 cut(s) 519, 602, 737
HphI GGTGA 2 cut(s) 16, 772
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 5 cut(s) 165, 280, 367, 733, 747
Hpy188III TCNNGA 7 cut(s) 53, 127, 269, 503, 584, 627, 680
Hpy8I GTNNAC 1 cut(s) 7
HpyAV CCTTC 1 cut(s) 215
HpyCH4III ACNGT 2 cut(s) 500, 581
HpyCH4V TGCA 3 cut(s) 307, 455, 471
HpyF3I CTNAG 5 cut(s) 162, 258, 279, 660, 744
Hsp92II CATG 1 cut(s) 518
HspAI GCGC 2 cut(s) 26, 563
Kzo9I GATC 3 cut(s) 143, 676, 720
LguI GCTCTTC 2 cut(s) 181, 693
LmnI GCTCC 1 cut(s) 368
Lsp1109I GCAGC 1 cut(s) 723
LweI GCATC 2 cut(s) 574, 625
MaeIII GTNAC 1 cut(s) 380
MalI GATC 3 cut(s) 145, 678, 722
MboI GATC 3 cut(s) 143, 676, 720
MfeI CAATTG 1 cut(s) 385
MflI RGATCY 1 cut(s) 143
MhlI GDGCHC 3 cut(s) 365, 486, 705
MluCI AATT 4 cut(s) 385, 449, 590, 793
MlyI GAGTC 1 cut(s) 611
MmeI TCCRAC 1 cut(s) 781
MnlI CCTC 8 cut(s) 171, 201, 309, 314, 434, 641, 739, 751
MseI TTAA 1 cut(s) 333
MspR9I CCNGG 3 cut(s) 141, 204, 216
MunI CAATTG 1 cut(s) 385
MvaI CCWGG 3 cut(s) 141, 204, 216
NdeI CATATG 1 cut(s) 467
NdeII GATC 3 cut(s) 143, 676, 720
NlaIII CATG 1 cut(s) 518
NlaIV GGNNCC 2 cut(s) 93, 445
NsbI TGCGCA 1 cut(s) 27
PciSI GCTCTTC 2 cut(s) 181, 693
PfeI GAWTC 2 cut(s) 519, 737
PkrI GCNGC 1 cut(s) 713
PleI GAGTC 1 cut(s) 610
PpsI GAGTC 1 cut(s) 610
PpuMI RGGWCCY 1 cut(s) 91
Psp124BI GAGCTC 1 cut(s) 365
Psp5II RGGWCCY 1 cut(s) 91
Psp6I CCWGG 3 cut(s) 139, 202, 214
PspGI CCWGG 3 cut(s) 139, 202, 214
PspN4I GGNNCC 2 cut(s) 93, 445
PspPI GGNCC 3 cut(s) 91, 444, 779
PspPPI RGGWCCY 1 cut(s) 91
PsuI RGATCY 1 cut(s) 143
SacI GAGCTC 1 cut(s) 365
SapI GCTCTTC 2 cut(s) 181, 693
SaqAI TTAA 1 cut(s) 333
SatI GCNGC 1 cut(s) 712
Sau3AI GATC 3 cut(s) 143, 676, 720
Sau96I GGNCC 3 cut(s) 91, 444, 779
SchI GAGTC 1 cut(s) 611
ScrFI CCNGG 3 cut(s) 141, 204, 216
SduI GDGCHC 3 cut(s) 365, 486, 705
SfaNI GCATC 2 cut(s) 574, 625
SinI GGWCC 3 cut(s) 91, 444, 779
Sse9I AATT 4 cut(s) 385, 449, 590, 793
SsiI CCGC 2 cut(s) 534, 552
SstI GAGCTC 1 cut(s) 365
StyD4I CCNGG 3 cut(s) 139, 202, 214
TaaI ACNGT 2 cut(s) 500, 581
TaqI TCGA 2 cut(s) 196, 756
TasI AATT 4 cut(s) 385, 449, 590, 793
TfiI GAWTC 2 cut(s) 519, 737
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
TscAI CASTG 2 cut(s) 157, 700
TseI GCWGC 1 cut(s) 711
TspDTI ATGAA 2 cut(s) 224, 454
TspRI CASTG 2 cut(s) 157, 700
VpaK11BI GGWCC 3 cut(s) 91, 444, 779
XagI CCTNNNNNAGG 1 cut(s) 219
XapI RAATTY 2 cut(s) 449, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.