MD01G1201600.v1.1

Actin depolymerisation factor/cofilin -like domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008202
Physical Location & Seq
Reverse (-)
29778802 .. 29779790
989 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1201600.v1.1.491

Sequence Viewer

Length: 408 bp
ATGGCGGTGTCTGGCATGGCCGTGAGTGATGAATGCAAACTCAAGTTCGGGGAGCTAAAAGCGAAGAGAAATTACCGATTCATTGTATTCAAGATTGACAAACAAGAAGTGGTGGTAGAGAAACTTGGCAGCCCAGATGAAACATATGAGGATTTTGCGGAGTCCCTGCCTGCTGATGAGTGCCGCTATGCTGTCTTCGATTTTGATTTCACCACTTCTGAGAACTGCCAGAAAAGCAAGATTTTCTTCATTGCATGGTCTCCTGAGATATCAAGGGTGAGAATGAAGATGGTGTATGCAAGCTCTAGAGATCGATTCAAGAGGGAACTTGACGGCATTCAAGTCGAGTTGCAGGCAACTGATCCGAGCGAAATGAGCCTCGACATTATTAAAGGCCGAGCTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF zf-CCHC zf-CCHC_4

Protein Analysis

136

Amino Acids

15.56

Weight (kDa)

5.15

Isoelectric Point (pI)

46.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 5 - 133 7.9e-41 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 5, 158, 184
AclWI GGATC 1 cut(s) 356
AcoI YGGCCR 1 cut(s) 18
AgsI TTSAA 3 cut(s) 91, 319, 341
AluBI AGCT 3 cut(s) 55, 303, 401
AluI AGCT 3 cut(s) 55, 303, 401
Alw21I GWGCWC 1 cut(s) 403
Alw26I GTCTC 1 cut(s) 264
AlwI GGATC 1 cut(s) 356
AoxI GGCC 2 cut(s) 18, 394
ApeKI GCWGC 1 cut(s) 129
AsuHPI GGTGA 2 cut(s) 202, 289
BanII GRGCYC 1 cut(s) 403
BarI GAAGNNNNNNTAC 2 cut(s) 56, 88
BbsI GAAGAC 1 cut(s) 187
Bbv12I GWGCWC 1 cut(s) 403
BbvI GCAGC 1 cut(s) 141
BccI CCATC 1 cut(s) 283
BceAI ACGGC 2 cut(s) 5, 349
BcgI CGANNNNNNTGC 2 cut(s) 325, 359
BcoDI GTCTC 1 cut(s) 264
BfaI CTAG 1 cut(s) 306
BisI GCNGC 2 cut(s) 130, 184
BlsI GCNGC 2 cut(s) 131, 185
BpiI GAAGAC 1 cut(s) 187
BpuEI CTTGAG 1 cut(s) 26
Bsa29I ATCGAT 1 cut(s) 313
BsaI GGTCTC 1 cut(s) 264
Bse3DI GCAATG 1 cut(s) 249
BseCI ATCGAT 1 cut(s) 313
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 2 cut(s) 210, 255
BseXI GCAGC 1 cut(s) 141
BshFI GGCC 2 cut(s) 20, 396
BshVI ATCGAT 1 cut(s) 313
BsiHKAI GWGCWC 1 cut(s) 403
BslFI GGGAC 1 cut(s) 148
BsmAI GTCTC 1 cut(s) 264
BsmFI GGGAC 1 cut(s) 148
BsmI GAATGC 2 cut(s) 38, 336
BsnI GGCC 2 cut(s) 20, 396
Bso31I GGTCTC 1 cut(s) 264
Bsp1286I GDGCHC 1 cut(s) 403
Bsp143I GATC 2 cut(s) 310, 361
BspACI CCGC 3 cut(s) 5, 158, 184
BspANI GGCC 2 cut(s) 20, 396
BspCNI CTCAG 2 cut(s) 211, 256
BspDI ATCGAT 1 cut(s) 313
BspPI GGATC 1 cut(s) 356
BspTNI GGTCTC 1 cut(s) 264
BsrDI GCAATG 1 cut(s) 249
BssMI GATC 2 cut(s) 310, 361
Bst6I CTCTTC 1 cut(s) 59
BstC8I GCNNGC 3 cut(s) 171, 301, 354
BstDEI CTNAG 2 cut(s) 219, 264
BstKTI GATC 2 cut(s) 313, 364
BstMAI GTCTC 1 cut(s) 264
BstMBI GATC 2 cut(s) 310, 361
BstMWI GCNNNNNNNGC 2 cut(s) 234, 375
BstV1I GCAGC 1 cut(s) 141
BstV2I GAAGAC 1 cut(s) 187
Bsu15I ATCGAT 1 cut(s) 313
BsuRI GGCC 2 cut(s) 20, 396
BsuTUI ATCGAT 1 cut(s) 313
Cac8I GCNNGC 3 cut(s) 171, 301, 354
ClaI ATCGAT 1 cut(s) 313
CviAII CATG 2 cut(s) 16, 255
CviJI RGCY 7 cut(s) 20, 55, 132, 303, 378, 396, 401
CviKI_1 RGCY 7 cut(s) 20, 55, 132, 303, 378, 396, 401
DdeI CTNAG 2 cut(s) 219, 264
DpnI GATC 2 cut(s) 312, 363
DpnII GATC 2 cut(s) 310, 361
EaeI YGGCCR 1 cut(s) 18
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Ecl136II GAGCTC 1 cut(s) 401
Eco24I GRGCYC 1 cut(s) 403
Eco31I GGTCTC 1 cut(s) 264
Eco32I GATATC 1 cut(s) 270
Eco53kI GAGCTC 1 cut(s) 401
EcoICRI GAGCTC 1 cut(s) 401
EcoRV GATATC 1 cut(s) 270
EcoT38I GRGCYC 1 cut(s) 403
FaeI CATG 2 cut(s) 19, 258
FaiI YATR 6 cut(s) 17, 145, 147, 189, 256, 297
FalI AAGNNNNNCTT 2 cut(s) 230, 262
FaqI GGGAC 1 cut(s) 148
FatI CATG 2 cut(s) 15, 254
FauNDI CATATG 1 cut(s) 145
Fnu4HI GCNGC 2 cut(s) 130, 184
FriOI GRGCYC 1 cut(s) 403
Fsp4HI GCNGC 2 cut(s) 130, 184
FspBI CTAG 1 cut(s) 306
GluI GCNGC 2 cut(s) 130, 184
HaeIII GGCC 2 cut(s) 20, 396
Hin1II CATG 2 cut(s) 19, 258
HinfI GANTC 3 cut(s) 78, 161, 315
HphI GGTGA 2 cut(s) 202, 289
Hpy188I TCNGA 2 cut(s) 220, 366
Hpy188III TCNNGA 4 cut(s) 91, 263, 306, 319
HpyCH4V TGCA 4 cut(s) 36, 254, 299, 352
HpyF10VI GCNNNNNNNGC 2 cut(s) 234, 375
HpyF3I CTNAG 2 cut(s) 219, 264
Hsp92II CATG 2 cut(s) 19, 258
Kzo9I GATC 2 cut(s) 310, 361
LmnI GCTCC 1 cut(s) 52
LpnPI CCDG 6 cut(s) 147, 179, 183, 242, 276, 338
Lsp1109I GCAGC 1 cut(s) 141
MaeI CTAG 1 cut(s) 306
MalI GATC 2 cut(s) 312, 363
MboI GATC 2 cut(s) 310, 361
MboII GAAGA 4 cut(s) 76, 187, 238, 298
MhlI GDGCHC 1 cut(s) 403
MluCI AATT 1 cut(s) 70
MlyI GAGTC 1 cut(s) 170
MnlI CCTC 3 cut(s) 142, 315, 389
MseI TTAA 2 cut(s) 390, 406
MslI CAYNNNNRTG 1 cut(s) 20
Mva1269I GAATGC 2 cut(s) 38, 336
MwoI GCNNNNNNNGC 2 cut(s) 234, 375
NdeI CATATG 1 cut(s) 145
NdeII GATC 2 cut(s) 310, 361
NlaIII CATG 2 cut(s) 19, 258
PctI GAATGC 2 cut(s) 38, 336
PfeI GAWTC 2 cut(s) 78, 315
PkrI GCNGC 2 cut(s) 131, 185
PleI GAGTC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 169
Psp124BI GAGCTC 1 cut(s) 403
RseI CAYNNNNRTG 1 cut(s) 20
SacI GAGCTC 1 cut(s) 403
SaqAI TTAA 2 cut(s) 390, 406
SatI GCNGC 2 cut(s) 130, 184
Sau3AI GATC 2 cut(s) 310, 361
SchI GAGTC 1 cut(s) 170
SduI GDGCHC 1 cut(s) 403
SetI ASST 3 cut(s) 57, 305, 403
SmiMI CAYNNNNRTG 1 cut(s) 20
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
Sse9I AATT 1 cut(s) 70
SsiI CCGC 3 cut(s) 5, 158, 184
SspMI CTAG 1 cut(s) 306
SstI GAGCTC 1 cut(s) 403
TaqI TCGA 4 cut(s) 198, 313, 345, 381
TasI AATT 1 cut(s) 70
TauI GCSGC 1 cut(s) 186
TfiI GAWTC 2 cut(s) 78, 315
Tru1I TTAA 2 cut(s) 390, 406
Tru9I TTAA 2 cut(s) 390, 406
TseI GCWGC 1 cut(s) 129
TspDTI ATGAA 5 cut(s) 45, 70, 153, 238, 299
XbaI TCTAGA 1 cut(s) 305
XspI CTAG 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.