Prupe.2G295100_v2.0.a1

Zinc knuckle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0008202
Physical Location & Seq
Reverse (-)
28637939 .. 28640507
2569 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G295100.1

Sequence Viewer

Length: 825 bp
ATGGTGAGCAAAAGACAGAGAGATGCTCGCAAGAGATTCAGGGCAGAAAACCCTGAACTGGTTAAACAGCCGACGCCGCCTAAGGACCCAAGCAAGAAGAAGGGAAAGAAGAGCACGTTCAAGCGCAAGAAAGCACCCAATGGTCCAGACAAACCCACCAAAACTTCTCATAGCAAACACCCACTTAGAGTCCCTGGTATGAGGCCTGGGGAGAGCTGTTTCATTTGTAAAGCCACCAACCATATCGCCAAGCTTTGCCCTGAGCGAGCTGGGTGGGACAAGCACAAGATATGTTTGTATTGTCGACAACGTGGGCATAGCATCAAGGATTGCCTTAACAAGAATGATGACACCGTGGCTGTGAAGTTATGTTATAATTGTGGGGAAACTGGGCATTCACTATCTACCTGCCCCCTACCTCTTCAAGAAGGAGGAACTAAATTTGCCAAGTGCTTCATCTGTAACGAGACCGGTCACTTAAGCAAGGACTGCTCTAAAAATACTCATGGGATTTATCCAAAGGGTGGTAGTTGTAAGATTTGTGGAGGCGTAACACATTTGGCTAGAGATTGTCCCAACAAAGGCAACAGAGATTCAATGGCTGTTGGCGGAGCTTTTCACTCATGGAATGAAAATCAAAGGCCAAGAGGAAAGGTTACCAAACTTGTGAGTGGAGACGATCTTGATGATGATTTCAATTTCGTAGATAAGAAACGTGGTGGTGCAACAGACGAGTCAGTTGAAAGTCCTGCTTCTCATGCGCAAGAAAGTCCTGTAAAATCAAAGAAGAAACAGGGACCCAAAGTAGTGAATTTTGTTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF zf-CCHC zf-CCHC_4

Protein Analysis

275

Amino Acids

30.17

Weight (kDa)

9.65

Isoelectric Point (pI)

34.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 375
Acc16I TGCGCA 1 cut(s) 762
Acc36I ACCTGC 1 cut(s) 416
AccB7I CCANNNNNTGG 1 cut(s) 524
AccI GTMKAC 1 cut(s) 304
AciI CCGC 2 cut(s) 77, 609
AcsI RAATTY 2 cut(s) 440, 811
AcyI GRCGYC 1 cut(s) 74
AfiI CCNNNNNNNGG 3 cut(s) 58, 524, 581
AflII CTTAAG 1 cut(s) 478
AgeI ACCGGT 1 cut(s) 470
AgsI TTSAA 5 cut(s) 121, 425, 597, 697, 743
AjnI CCWGG 2 cut(s) 193, 205
AluBI AGCT 4 cut(s) 216, 253, 269, 614
AluI AGCT 4 cut(s) 216, 253, 269, 614
Alw21I GWGCWC 1 cut(s) 116
Alw26I GTCTC 2 cut(s) 461, 669
AoxI GGCC 2 cut(s) 203, 641
ApoI RAATTY 2 cut(s) 440, 811
AsiGI ACCGGT 1 cut(s) 470
AspLEI GCGC 2 cut(s) 126, 763
AspS9I GGNCC 3 cut(s) 85, 143, 797
AsuHPI GGTGA 1 cut(s) 16
AvaII GGWCC 3 cut(s) 85, 143, 797
AxyI CCTNAGG 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 116
BciT130I CCWGG 2 cut(s) 195, 207
BcoDI GTCTC 2 cut(s) 461, 669
BfaI CTAG 1 cut(s) 564
BfrI CTTAAG 1 cut(s) 478
BfuAI ACCTGC 1 cut(s) 416
BisI GCNGC 1 cut(s) 77
BlsI GCNGC 1 cut(s) 78
Bme1390I CCNGG 2 cut(s) 195, 207
Bme18I GGWCC 3 cut(s) 85, 143, 797
BmgT120I GGNCC 3 cut(s) 85, 143, 797
BmiI GGNNCC 3 cut(s) 87, 798, 799
BmrFI CCNGG 2 cut(s) 195, 207
BmrI ACTGGG 1 cut(s) 399
BmsI GCATC 2 cut(s) 13, 330
BmuI ACTGGG 1 cut(s) 399
BplI GAGNNNNNCTC 2 cut(s) 10, 42
Bpu10I CCTNAGC 1 cut(s) 261
BsaHI GRCGYC 1 cut(s) 74
BsaI GGTCTC 1 cut(s) 461
BsaJI CCNNGG 3 cut(s) 193, 206, 354
BsaWI WCCGGW 1 cut(s) 470
Bsc4I CCNNNNNNNGG 3 cut(s) 58, 524, 581
Bse118I RCCGGY 1 cut(s) 470
Bse1I ACTGG 2 cut(s) 63, 394
Bse21I CCTNAGG 1 cut(s) 81
BseBI CCWGG 2 cut(s) 195, 207
BseDI CCNNGG 3 cut(s) 193, 206, 354
BseLI CCNNNNNNNGG 3 cut(s) 58, 524, 581
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 2 cut(s) 63, 394
BseYI CCCAGC 1 cut(s) 269
BshFI GGCC 2 cut(s) 205, 643
BshTI ACCGGT 1 cut(s) 470
BsiHKAI GWGCWC 1 cut(s) 116
BsiSI CCGG 1 cut(s) 471
BslFI GGGAC 4 cut(s) 176, 290, 558, 810
BslI CCNNNNNNNGG 3 cut(s) 58, 524, 581
BsmAI GTCTC 2 cut(s) 461, 669
BsmBI CGTCTC 1 cut(s) 669
BsmFI GGGAC 4 cut(s) 176, 290, 558, 810
BsmI GAATGC 1 cut(s) 394
BsnI GGCC 2 cut(s) 205, 643
Bso31I GGTCTC 1 cut(s) 461
Bsp1286I GDGCHC 1 cut(s) 116
Bsp143I GATC 1 cut(s) 679
BspACI CCGC 2 cut(s) 77, 609
BspANI GGCC 2 cut(s) 205, 643
BspCNI CTCAG 1 cut(s) 253
BspLI GGNNCC 3 cut(s) 87, 798, 799
BspMI ACCTGC 1 cut(s) 416
BspQI GCTCTTC 1 cut(s) 104
BspTI CTTAAG 1 cut(s) 478
BspTNI GGTCTC 1 cut(s) 461
BsrFI RCCGGY 1 cut(s) 470
BsrI ACTGG 2 cut(s) 63, 394
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 3 cut(s) 193, 206, 354
BssMI GATC 1 cut(s) 679
BssNI GRCGYC 1 cut(s) 74
Bst2UI CCWGG 2 cut(s) 195, 207
Bst4CI ACNGT 1 cut(s) 355
Bst6I CTCTTC 2 cut(s) 104, 426
BstACI GRCGYC 1 cut(s) 74
BstAFI CTTAAG 1 cut(s) 478
BstAPI GCANNNNNTGC 1 cut(s) 489
BstC8I GCNNGC 2 cut(s) 28, 267
BstDEI CTNAG 3 cut(s) 81, 185, 261
BstDSI CCRYGG 1 cut(s) 354
BstEII GGTNACC 1 cut(s) 655
BstHHI GCGC 2 cut(s) 126, 763
BstKTI GATC 1 cut(s) 682
BstMAI GTCTC 2 cut(s) 461, 669
BstMBI GATC 1 cut(s) 679
BstMWI GCNNNNNNNGC 3 cut(s) 76, 489, 758
BstNI CCWGG 2 cut(s) 195, 207
BstPI GGTNACC 1 cut(s) 655
BstSCI CCNGG 2 cut(s) 193, 205
Bsu36I CCTNAGG 1 cut(s) 81
BsuRI GGCC 2 cut(s) 205, 643
BtgI CCRYGG 1 cut(s) 354
BveI ACCTGC 1 cut(s) 416
Cac8I GCNNGC 2 cut(s) 28, 267
CfoI GCGC 2 cut(s) 126, 763
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 3 cut(s) 85, 143, 797
CseI GACGC 1 cut(s) 82
CspAI ACCGGT 1 cut(s) 470
CviAII CATG 3 cut(s) 506, 624, 758
DdeI CTNAG 3 cut(s) 81, 185, 261
DpnI GATC 1 cut(s) 681
DpnII GATC 1 cut(s) 679
Eam1104I CTCTTC 2 cut(s) 104, 426
EarI CTCTTC 2 cut(s) 104, 426
EciI GGCGGA 1 cut(s) 624
Eco147I AGGCCT 1 cut(s) 205
Eco31I GGTCTC 1 cut(s) 461
Eco47I GGWCC 3 cut(s) 85, 143, 797
Eco81I CCTNAGG 1 cut(s) 81
Eco91I GGTNACC 1 cut(s) 655
EcoO109I RGGNCCY 2 cut(s) 85, 797
EcoO65I GGTNACC 1 cut(s) 655
EcoRII CCWGG 2 cut(s) 193, 205
Esp3I CGTCTC 1 cut(s) 669
FaeI CATG 3 cut(s) 509, 627, 761
FalI AAGNNNNNCTT 2 cut(s) 736, 768
FaqI GGGAC 4 cut(s) 176, 290, 558, 810
FatI CATG 3 cut(s) 505, 623, 757
FblI GTMKAC 1 cut(s) 304
Fnu4HI GCNGC 1 cut(s) 77
Fsp4HI GCNGC 1 cut(s) 77
FspBI CTAG 1 cut(s) 564
FspI TGCGCA 1 cut(s) 762
GlaI GCGC 2 cut(s) 125, 762
GluI GCNGC 1 cut(s) 77
GsaI CCCAGC 1 cut(s) 273
HaeIII GGCC 2 cut(s) 205, 643
HapII CCGG 1 cut(s) 471
HgaI GACGC 1 cut(s) 82
HhaI GCGC 2 cut(s) 126, 763
Hin1I GRCGYC 1 cut(s) 74
Hin1II CATG 3 cut(s) 509, 627, 761
Hin6I GCGC 2 cut(s) 124, 761
HinP1I GCGC 2 cut(s) 124, 761
HincII GTYRAC 1 cut(s) 305
HindII GTYRAC 1 cut(s) 305
HindIII AAGCTT 1 cut(s) 251
HinfI GANTC 4 cut(s) 36, 189, 593, 734
HpaII CCGG 1 cut(s) 471
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 305
Hpy188III TCNNGA 3 cut(s) 146, 425, 683
Hpy8I GTNNAC 1 cut(s) 305
Hpy99I CGWCG 1 cut(s) 76
HpyAV CCTTC 2 cut(s) 94, 422
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4IV ACGT 3 cut(s) 116, 310, 715
HpyCH4V TGCA 1 cut(s) 725
HpyF10VI GCNNNNNNNGC 3 cut(s) 76, 489, 758
HpyF3I CTNAG 3 cut(s) 81, 185, 261
HpySE526I ACGT 3 cut(s) 116, 310, 715
Hsp92I GRCGYC 1 cut(s) 74
Hsp92II CATG 3 cut(s) 509, 627, 761
HspAI GCGC 2 cut(s) 124, 761
KflI GGGWCCC 1 cut(s) 797
Kzo9I GATC 1 cut(s) 679
LguI GCTCTTC 1 cut(s) 104
LmnI GCTCC 1 cut(s) 611
LweI GCATC 2 cut(s) 13, 330
MaeI CTAG 1 cut(s) 564
MaeII ACGT 3 cut(s) 116, 310, 715
MaeIII GTNAC 4 cut(s) 461, 473, 550, 655
MalI GATC 1 cut(s) 681
MboI GATC 1 cut(s) 679
MboII GAAGA 4 cut(s) 109, 121, 413, 799
MhlI GDGCHC 1 cut(s) 116
MluCI AATT 4 cut(s) 376, 440, 697, 811
MlyI GAGTC 2 cut(s) 198, 743
MmeI TCCRAC 1 cut(s) 799
MnlI CCTC 5 cut(s) 195, 425, 429, 539, 641
MseI TTAA 3 cut(s) 63, 336, 479
MspCI CTTAAG 1 cut(s) 478
MspI CCGG 1 cut(s) 471
MspR9I CCNGG 2 cut(s) 195, 207
Mva1269I GAATGC 1 cut(s) 394
MvaI CCWGG 2 cut(s) 195, 207
MwoI GCNNNNNNNGC 3 cut(s) 76, 489, 758
NdeII GATC 1 cut(s) 679
NlaIII CATG 3 cut(s) 509, 627, 761
NlaIV GGNNCC 3 cut(s) 87, 798, 799
NmuCI GTSAC 1 cut(s) 473
NsbI TGCGCA 1 cut(s) 762
PceI AGGCCT 1 cut(s) 205
PciSI GCTCTTC 1 cut(s) 104
PctI GAATGC 1 cut(s) 394
PfeI GAWTC 2 cut(s) 36, 593
PflMI CCANNNNNTGG 1 cut(s) 524
PinAI ACCGGT 1 cut(s) 470
PkrI GCNGC 1 cut(s) 78
PleI GAGTC 2 cut(s) 197, 742
PpsI GAGTC 2 cut(s) 197, 742
PpuMI RGGWCCY 2 cut(s) 85, 797
PsiI TTATAA 1 cut(s) 375
Psp5II RGGWCCY 2 cut(s) 85, 797
Psp6I CCWGG 2 cut(s) 193, 205
PspEI GGTNACC 1 cut(s) 655
PspFI CCCAGC 1 cut(s) 269
PspGI CCWGG 2 cut(s) 193, 205
PspN4I GGNNCC 3 cut(s) 87, 798, 799
PspPI GGNCC 3 cut(s) 85, 143, 797
PspPPI RGGWCCY 2 cut(s) 85, 797
SalI GTCGAC 1 cut(s) 303
SapI GCTCTTC 1 cut(s) 104
SaqAI TTAA 3 cut(s) 63, 336, 479
SatI GCNGC 1 cut(s) 77
Sau3AI GATC 1 cut(s) 679
Sau96I GGNCC 3 cut(s) 85, 143, 797
SchI GAGTC 2 cut(s) 198, 743
ScrFI CCNGG 2 cut(s) 195, 207
SduI GDGCHC 1 cut(s) 116
SfaNI GCATC 2 cut(s) 13, 330
SinI GGWCC 3 cut(s) 85, 143, 797
SmlI CTYRAG 1 cut(s) 478
SmoI CTYRAG 1 cut(s) 478
Sse9I AATT 4 cut(s) 376, 440, 697, 811
SseBI AGGCCT 1 cut(s) 205
SsiI CCGC 2 cut(s) 77, 609
SspMI CTAG 1 cut(s) 564
StuI AGGCCT 1 cut(s) 205
StyD4I CCNGG 2 cut(s) 193, 205
TaaI ACNGT 1 cut(s) 355
TaiI ACGT 3 cut(s) 119, 313, 718
TaqI TCGA 1 cut(s) 304
TasI AATT 4 cut(s) 376, 440, 697, 811
TauI GCSGC 1 cut(s) 79
TfiI GAWTC 2 cut(s) 36, 593
Tru1I TTAA 3 cut(s) 63, 336, 479
Tru9I TTAA 3 cut(s) 63, 336, 479
TseFI GTSAC 1 cut(s) 473
Tsp45I GTSAC 1 cut(s) 473
TspDTI ATGAA 3 cut(s) 211, 445, 645
Van91I CCANNNNNTGG 1 cut(s) 524
Vha464I CTTAAG 1 cut(s) 478
VpaK11BI GGWCC 3 cut(s) 85, 143, 797
XapI RAATTY 2 cut(s) 440, 811
XmiI GTMKAC 1 cut(s) 304
XspI CTAG 1 cut(s) 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.