Rh1CG393900

Actin depolymerisation factor/cofilin -like domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0008202
Physical Location & Seq
Reverse (-)
66127397 .. 66129730
2334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG393900.1

Sequence Viewer

Length: 846 bp
ATGGCGAACAAAAGACAGAGAGAGGCACGCAAGAGATTCAGAGCAGAGCACCCAGAACTGGTTAAAACCCCAGAGCCAACTCCCCCAAAAGACCCAAGCAAGAAGAAGAAGGTAAAGAAGCCCAGTTTCAAGCGCAAGAGATCAGAATCTGGAGCACCCAATGACCCCAATAGAGCAGCCAAGAAGTCTCGGAGTAAACACCCACTTAGAGTCCCTGGTATGAAGCCCGGAGATAGCTGCTTCATTTGTAAAGGCACTGACCATATTGCCAAGCTTTGCCCTGAGAAAGCTGAGTGGGATAGGCACAAGATATGCTTGTTTTGTCGACAACGCGGGCATAGTCTGAAGAATTGCCAAAAGAAGAATGAGAATGATACGGAGGCGAAGTTATGTTATAATTGTGGGGAAACCGGGCATTCGCTTTCTAGATGCCCCTTACCTCTTCAAGATGGGGGAACTAAATTTGCCAAGTGCTTCGTCTGTAACGAGACTGGTCACCTGAGCAAGGACTGTCCTAAAAACTCTCATGGGATTTATCCAAAGGGTGGTTCTTGTAAAATATGTGGCGGTGTAACACATTTGGCACGAGACTGTCCTAACAAAGACAGCAGGAATTCAATAGGTTCTGGCAATTCTAATCCCAAATGGAATGAACCAAGAGGAAAGGTTACCAAATTTGCGAGTGGAGATGATCTTGAGGATGATTTCAGTTTCATAGGTGAGAAGACTGTTGCCAAAACAGTAGACGAGTCTGCCAATGTAGTTGGTGCTGCTTCTCTTCCACAAGAAAGTAACGTAAAATCAAAGAAGAAACAGGGTCCCAAAATAGTAAATTTTGCCGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF zf-CCHC zf-CCHC_4

Protein Analysis

281

Amino Acids

30.98

Weight (kDa)

9.66

Isoelectric Point (pI)

39.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC PF00098 157 - 173 3e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 396
AccB7I CCANNNNNTGG 1 cut(s) 545
AccI GTMKAC 2 cut(s) 325, 744
AccII CGCG 1 cut(s) 333
AciI CCGC 2 cut(s) 333, 567
AcsI RAATTY 4 cut(s) 461, 613, 674, 832
AcuI CTGAAG 1 cut(s) 365
AfiI CCNNNNNNNGG 3 cut(s) 58, 505, 545
AgsI TTSAA 3 cut(s) 130, 446, 618
AjnI CCWGG 1 cut(s) 214
AjuI GAANNNNNNNTTGG 2 cut(s) 532, 564
AluBI AGCT 3 cut(s) 237, 274, 290
AluI AGCT 3 cut(s) 237, 274, 290
Alw21I GWGCWC 2 cut(s) 51, 157
Alw26I GTCTC 3 cut(s) 192, 482, 582
AlwNI CAGNNNCTG 1 cut(s) 149
ApeKI GCWGC 3 cut(s) 176, 237, 770
ApoI RAATTY 4 cut(s) 461, 613, 674, 832
AspLEI GCGC 1 cut(s) 135
AspS9I GGNCC 1 cut(s) 818
AsuC2I CCSGG 2 cut(s) 228, 412
AsuHPI GGTGA 2 cut(s) 488, 731
AvaII GGWCC 1 cut(s) 818
BauI CACGAG 1 cut(s) 585
BbsI GAAGAC 1 cut(s) 731
Bbv12I GWGCWC 2 cut(s) 51, 157
BbvI GCAGC 3 cut(s) 188, 224, 757
BccI CCATC 1 cut(s) 443
BciT130I CCWGG 1 cut(s) 216
BcnI CCSGG 2 cut(s) 228, 412
BcoDI GTCTC 3 cut(s) 192, 482, 582
BfaI CTAG 1 cut(s) 426
BisI GCNGC 3 cut(s) 177, 238, 771
BlsI GCNGC 3 cut(s) 178, 239, 772
Bme1390I CCNGG 3 cut(s) 216, 228, 412
Bme18I GGWCC 1 cut(s) 818
BmgT120I GGNCC 1 cut(s) 818
BmiI GGNNCC 2 cut(s) 819, 820
BmrFI CCNGG 3 cut(s) 216, 228, 412
BmrI ACTGGG 1 cut(s) 117
BmsI GCATC 1 cut(s) 419
BmuI ACTGGG 1 cut(s) 117
BpiI GAAGAC 1 cut(s) 731
BpmI CTGGAG 1 cut(s) 171
Bpu10I CCTNAGC 1 cut(s) 500
BpuEI CTTGAG 1 cut(s) 716
BpuMI CCSGG 2 cut(s) 228, 412
BsaBI GATNNNNATC 1 cut(s) 145
BsaJI CCNNGG 1 cut(s) 214
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
Bsc4I CCNNNNNNNGG 3 cut(s) 58, 505, 545
Bse1I ACTGG 3 cut(s) 63, 123, 496
Bse8I GATNNNNATC 1 cut(s) 145
BseBI CCWGG 1 cut(s) 216
BseDI CCNNGG 1 cut(s) 214
BseGI GGATG 1 cut(s) 706
BseJI GATNNNNATC 1 cut(s) 145
BseLI CCNNNNNNNGG 3 cut(s) 58, 505, 545
BseMII CTCAG 3 cut(s) 273, 282, 491
BseNI ACTGG 3 cut(s) 63, 123, 496
BseXI GCAGC 3 cut(s) 188, 224, 757
Bsh1236I CGCG 1 cut(s) 333
BsiHKAI GWGCWC 2 cut(s) 51, 157
BsiSI CCGG 3 cut(s) 228, 411, 840
BslFI GGGAC 2 cut(s) 197, 804
BslI CCNNNNNNNGG 3 cut(s) 58, 505, 545
BsmAI GTCTC 3 cut(s) 192, 482, 582
BsmFI GGGAC 2 cut(s) 197, 804
BsmI GAATGC 1 cut(s) 415
Bsp1286I GDGCHC 2 cut(s) 51, 157
Bsp143I GATC 2 cut(s) 140, 691
BspACI CCGC 2 cut(s) 333, 567
BspCNI CTCAG 3 cut(s) 274, 283, 492
BspFNI CGCG 1 cut(s) 333
BspLI GGNNCC 2 cut(s) 819, 820
BsrI ACTGG 3 cut(s) 63, 123, 496
BssECI CCNNGG 1 cut(s) 214
BssMI GATC 2 cut(s) 140, 691
BssSI CACGAG 1 cut(s) 585
Bst2BI CACGAG 1 cut(s) 585
Bst2UI CCWGG 1 cut(s) 216
Bst4CI ACNGT 4 cut(s) 512, 593, 730, 742
Bst6I CTCTTC 2 cut(s) 447, 783
BstC8I GCNNGC 2 cut(s) 28, 335
BstDEI CTNAG 4 cut(s) 206, 282, 291, 500
BstEII GGTNACC 2 cut(s) 494, 667
BstENI CCTNNNNNAGG 1 cut(s) 503
BstF5I GGATG 1 cut(s) 706
BstFNI CGCG 1 cut(s) 333
BstHHI GCGC 1 cut(s) 135
BstKTI GATC 2 cut(s) 143, 694
BstMAI GTCTC 3 cut(s) 192, 482, 582
BstMBI GATC 2 cut(s) 140, 691
BstNI CCWGG 1 cut(s) 216
BstPI GGTNACC 2 cut(s) 494, 667
BstSCI CCNGG 3 cut(s) 214, 226, 410
BstUI CGCG 1 cut(s) 333
BstV1I GCAGC 3 cut(s) 188, 224, 757
BstV2I GAAGAC 1 cut(s) 731
BtsCI GGATG 1 cut(s) 706
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 2 cut(s) 28, 335
CaiI CAGNNNCTG 1 cut(s) 149
CfoI GCGC 1 cut(s) 135
Cfr13I GGNCC 1 cut(s) 818
CviAII CATG 1 cut(s) 527
CviJI RGCY 7 cut(s) 76, 121, 179, 226, 237, 274, 290
CviKI_1 RGCY 7 cut(s) 76, 121, 179, 226, 237, 274, 290
DdeI CTNAG 4 cut(s) 206, 282, 291, 500
DpnI GATC 2 cut(s) 142, 693
DpnII GATC 2 cut(s) 140, 691
Eam1104I CTCTTC 2 cut(s) 447, 783
EarI CTCTTC 2 cut(s) 447, 783
Eco47I GGWCC 1 cut(s) 818
Eco57I CTGAAG 1 cut(s) 365
Eco91I GGTNACC 2 cut(s) 494, 667
EcoNI CCTNNNNNAGG 1 cut(s) 503
EcoO109I RGGNCCY 1 cut(s) 818
EcoO65I GGTNACC 2 cut(s) 494, 667
EcoRI GAATTC 1 cut(s) 613
EcoRII CCWGG 1 cut(s) 214
FaeI CATG 1 cut(s) 530
FaiI YATR 9 cut(s) 221, 264, 313, 339, 391, 396, 528, 562, 716
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FaqI GGGAC 2 cut(s) 197, 804
FatI CATG 1 cut(s) 526
FauI CCCGC 1 cut(s) 326
FblI GTMKAC 2 cut(s) 325, 744
Fnu4HI GCNGC 3 cut(s) 177, 238, 771
FokI GGATG 1 cut(s) 713
Fsp4HI GCNGC 3 cut(s) 177, 238, 771
FspBI CTAG 1 cut(s) 426
GlaI GCGC 1 cut(s) 134
GluI GCNGC 3 cut(s) 177, 238, 771
GsuI CTGGAG 1 cut(s) 171
HapII CCGG 3 cut(s) 228, 411, 840
HhaI GCGC 1 cut(s) 135
Hin1II CATG 1 cut(s) 530
Hin6I GCGC 1 cut(s) 133
HinP1I GCGC 1 cut(s) 133
HincII GTYRAC 1 cut(s) 326
HindII GTYRAC 1 cut(s) 326
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 4 cut(s) 36, 146, 210, 749
HpaII CCGG 3 cut(s) 228, 411, 840
HphI GGTGA 2 cut(s) 488, 731
Hpy166II GTNNAC 3 cut(s) 197, 326, 745
Hpy188I TCNGA 4 cut(s) 41, 145, 192, 345
Hpy188III TCNNGA 4 cut(s) 150, 426, 446, 695
Hpy8I GTNNAC 3 cut(s) 197, 326, 745
HpyAV CCTTC 1 cut(s) 103
HpyCH4III ACNGT 4 cut(s) 512, 593, 730, 742
HpyCH4IV ACGT 1 cut(s) 795
HpyF3I CTNAG 4 cut(s) 206, 282, 291, 500
HpySE526I ACGT 1 cut(s) 795
Hsp92II CATG 1 cut(s) 530
HspAI GCGC 1 cut(s) 133
KflI GGGWCCC 1 cut(s) 818
Kzo9I GATC 2 cut(s) 140, 691
LmnI GCTCC 1 cut(s) 152
Lsp1109I GCAGC 3 cut(s) 188, 224, 757
LweI GCATC 1 cut(s) 419
MaeI CTAG 1 cut(s) 426
MaeII ACGT 1 cut(s) 795
MaeIII GTNAC 5 cut(s) 482, 494, 571, 667, 791
MalI GATC 2 cut(s) 142, 693
MboI GATC 2 cut(s) 140, 691
MboII GAAGA 8 cut(s) 115, 118, 358, 373, 434, 736, 770, 820
MhlI GDGCHC 2 cut(s) 51, 157
MluCI AATT 7 cut(s) 349, 397, 461, 613, 631, 674, 832
MlyI GAGTC 2 cut(s) 219, 758
MnlI CCTC 5 cut(s) 16, 373, 450, 653, 691
MseI TTAA 1 cut(s) 63
MspI CCGG 3 cut(s) 228, 411, 840
MspR9I CCNGG 3 cut(s) 216, 228, 412
Mva1269I GAATGC 1 cut(s) 415
MvaI CCWGG 1 cut(s) 216
MvnI CGCG 1 cut(s) 333
NciI CCSGG 2 cut(s) 228, 412
NdeII GATC 2 cut(s) 140, 691
NlaIII CATG 1 cut(s) 530
NlaIV GGNNCC 2 cut(s) 819, 820
NmuCI GTSAC 1 cut(s) 494
PcsI WCGNNNNNNNCGW 1 cut(s) 483
PctI GAATGC 1 cut(s) 415
PfeI GAWTC 2 cut(s) 36, 146
PflMI CCANNNNNTGG 1 cut(s) 545
PkrI GCNGC 3 cut(s) 178, 239, 772
PleI GAGTC 2 cut(s) 218, 757
PpsI GAGTC 2 cut(s) 218, 757
PpuMI RGGWCCY 1 cut(s) 818
PsiI TTATAA 1 cut(s) 396
Psp5II RGGWCCY 1 cut(s) 818
Psp6I CCWGG 1 cut(s) 214
PspEI GGTNACC 2 cut(s) 494, 667
PspGI CCWGG 1 cut(s) 214
PspN4I GGNNCC 2 cut(s) 819, 820
PspPI GGNCC 1 cut(s) 818
PspPPI RGGWCCY 1 cut(s) 818
PstNI CAGNNNCTG 1 cut(s) 149
SalI GTCGAC 1 cut(s) 324
SaqAI TTAA 1 cut(s) 63
SatI GCNGC 3 cut(s) 177, 238, 771
Sau3AI GATC 2 cut(s) 140, 691
Sau96I GGNCC 1 cut(s) 818
SchI GAGTC 2 cut(s) 219, 758
ScrFI CCNGG 3 cut(s) 216, 228, 412
SduI GDGCHC 2 cut(s) 51, 157
SfaNI GCATC 1 cut(s) 419
SinI GGWCC 1 cut(s) 818
SmlI CTYRAG 1 cut(s) 695
SmoI CTYRAG 1 cut(s) 695
Sse9I AATT 7 cut(s) 349, 397, 461, 613, 631, 674, 832
SsiI CCGC 2 cut(s) 333, 567
SspMI CTAG 1 cut(s) 426
StyD4I CCNGG 3 cut(s) 214, 226, 410
TaaI ACNGT 4 cut(s) 512, 593, 730, 742
TaiI ACGT 1 cut(s) 798
TaqI TCGA 1 cut(s) 325
TasI AATT 7 cut(s) 349, 397, 461, 613, 631, 674, 832
TfiI GAWTC 2 cut(s) 36, 146
Tru1I TTAA 1 cut(s) 63
Tru9I TTAA 1 cut(s) 63
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 1 cut(s) 494
TseI GCWGC 3 cut(s) 176, 237, 770
Tsp45I GTSAC 1 cut(s) 494
TspDTI ATGAA 4 cut(s) 232, 236, 666, 703
TspGWI ACGGA 1 cut(s) 392
TspRI CASTG 1 cut(s) 262
Van91I CCANNNNNTGG 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 818
XagI CCTNNNNNAGG 1 cut(s) 503
XapI RAATTY 4 cut(s) 461, 613, 674, 832
XbaI TCTAGA 1 cut(s) 425
XmiI GTMKAC 2 cut(s) 325, 744
XspI CTAG 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.