RLG00000026522

Actin depolymerisation factor/cofilin -like domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0008202
Physical Location & Seq
Forward (+)
3563638 .. 3566872
3235 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026522

Sequence Viewer

Length: 1023 bp
ATGGCGAACAAAAGACAGAGAGAGGCACGCAAGAGATTCAGAGCAGAGCACCCAGAACTGGTTAAAACCCCAGAGCCAACTCCCCCAAAAGACCCAATCAAGAAGAAGAAGGTAAAGAAGCCCAGTTTCAAGCGCAAGAGATCAGAATCTGGAGCACCCAATGACCCCAATAGAGCAGCCAAGAAGTCTCGGAGTAAACACCCACTTAGAGTCCCTGGTATGAAGCCTGGTGATAGCTGCTTCATTTGTAAAGGCACTGACCATATTGCCAAGCTTTGCCCTGAGAAAGCTGAGTGGGATAGGCACAAGATTTGCTTGTTTTGTCGACAACGCGGGCATAGTCTGAAGAATTGCCAAAAGAAGAATGAGAATGATATGGAGGCGAAGTTATGTTATAATTGTGGGGAAACTGGGCATTCGCTTTCGAGATGCCCCTTACCTCTTCAAGATGGGAATGAACCAAGAGGAAAGGTTACCAAATTTGCGAGTGGAGATGATCTTGAGGATGATTTCAGTTTCATAGGTGAGAAGACTGTTGCCACAACAGTAGACGAGTCTGCCAATGTAGTTGGTACTGCTTCTCTTCCACAAGAAAGTAACGCCGCATCGCCTGGCTTTGCAGTGTCTGGCCTAGCCGTGAATGAGGAGTGCAAGCAGAAGTTCTTGGAGCTAAAAGCCAAGAGAAACCATCGTTTCATTATATTCAAGATTGATAAGCAAGAAGTGGTGGTAGAGAAACTTGGAGAGCCAGACGAAACTTACGATGATTTCACAGCCTCGCTGCCTGCGGAGGAGTGCCGCTATGCTGTGTTTGATCTTGATTTCACCACCATTGAGAACTGCCAGAAAAGCAAGATTTTCTTCATTGCATGGTCACCTGATACATCAAAGGTGAGAATGAAGATGGTGTATGCTAGCTCCAAAGATAGATTCAAGAGAGAATTGGATGGCATTCAAGTTGAGTTGCAAGCAACTGATCCAAGTGAACTGAGCTTTGACATTATAAAATCCCGAGCTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF zf-CCHC zf-CCHC_4

Protein Analysis

341

Amino Acids

38.43

Weight (kDa)

9.06

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 211 - 338 5e-42 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 396, 1004
AccI GTMKAC 2 cut(s) 325, 549
AccII CGCG 1 cut(s) 333
AciI CCGC 4 cut(s) 333, 603, 788, 799
AclWI GGATC 1 cut(s) 971
AcsI RAATTY 1 cut(s) 479
AcuI CTGAAG 1 cut(s) 365
AfaI GTAC 1 cut(s) 574
AfiI CCNNNNNNNGG 1 cut(s) 58
AgsI TTSAA 5 cut(s) 130, 446, 706, 934, 956
AjnI CCWGG 3 cut(s) 214, 226, 610
AjuI GAANNNNNNNTTGG 2 cut(s) 914, 946
AluBI AGCT 7 cut(s) 237, 274, 290, 670, 918, 993, 1016
AluI AGCT 7 cut(s) 237, 274, 290, 670, 918, 993, 1016
Alw21I GWGCWC 3 cut(s) 51, 157, 1018
Alw26I GTCTC 1 cut(s) 192
AlwI GGATC 1 cut(s) 971
AlwNI CAGNNNCTG 2 cut(s) 149, 626
Ama87I CYCGRG 1 cut(s) 1011
AoxI GGCC 1 cut(s) 628
ApeKI GCWGC 3 cut(s) 176, 237, 781
ApoI RAATTY 1 cut(s) 479
AspLEI GCGC 1 cut(s) 135
AsuHPI GGTGA 5 cut(s) 242, 536, 817, 867, 904
AsuNHI GCTAGC 1 cut(s) 914
AvaI CYCGRG 1 cut(s) 1011
BanII GRGCYC 1 cut(s) 1018
BbsI GAAGAC 1 cut(s) 536
Bbv12I GWGCWC 3 cut(s) 51, 157, 1018
BbvI GCAGC 3 cut(s) 188, 224, 768
BccI CCATC 4 cut(s) 443, 696, 898, 941
BceAI ACGGC 1 cut(s) 620
BciT130I CCWGG 3 cut(s) 216, 228, 612
BcoDI GTCTC 1 cut(s) 192
BfaI CTAG 2 cut(s) 632, 915
BisI GCNGC 5 cut(s) 177, 238, 603, 782, 799
BlsI GCNGC 5 cut(s) 178, 239, 604, 783, 800
Bme1390I CCNGG 3 cut(s) 216, 228, 612
BmeT110I CYCGRG 1 cut(s) 1011
BmrFI CCNGG 3 cut(s) 216, 228, 612
BmrI ACTGGG 2 cut(s) 117, 420
BmsI GCATC 2 cut(s) 419, 614
BmtI GCTAGC 1 cut(s) 918
BmuI ACTGGG 2 cut(s) 117, 420
BpiI GAAGAC 1 cut(s) 536
BpmI CTGGAG 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 521
BsaBI GATNNNNATC 1 cut(s) 145
BsaJI CCNNGG 1 cut(s) 214
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
Bsc4I CCNNNNNNNGG 1 cut(s) 58
Bse1I ACTGG 3 cut(s) 63, 123, 415
Bse3DI GCAATG 1 cut(s) 864
Bse8I GATNNNNATC 1 cut(s) 145
BseBI CCWGG 3 cut(s) 216, 228, 612
BseDI CCNNGG 1 cut(s) 214
BseGI GGATG 2 cut(s) 511, 952
BseJI GATNNNNATC 1 cut(s) 145
BseLI CCNNNNNNNGG 1 cut(s) 58
BseMI GCAATG 1 cut(s) 864
BseMII CTCAG 3 cut(s) 273, 282, 980
BseNI ACTGG 3 cut(s) 63, 123, 415
BseRI GAGGAG 2 cut(s) 659, 806
BseXI GCAGC 3 cut(s) 188, 224, 768
Bsh1236I CGCG 1 cut(s) 333
BshFI GGCC 1 cut(s) 630
BsiHKAI GWGCWC 3 cut(s) 51, 157, 1018
BsiHKCI CYCGRG 1 cut(s) 1011
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 1 cut(s) 58
BsmAI GTCTC 1 cut(s) 192
BsmFI GGGAC 1 cut(s) 197
BsmI GAATGC 2 cut(s) 415, 951
BsnI GGCC 1 cut(s) 630
BsoBI CYCGRG 1 cut(s) 1011
Bsp1286I GDGCHC 3 cut(s) 51, 157, 1018
Bsp143I GATC 4 cut(s) 140, 496, 814, 976
BspACI CCGC 4 cut(s) 333, 603, 788, 799
BspANI GGCC 1 cut(s) 630
BspCNI CTCAG 3 cut(s) 274, 283, 981
BspFNI CGCG 1 cut(s) 333
BspOI GCTAGC 1 cut(s) 918
BspPI GGATC 1 cut(s) 971
BsrDI GCAATG 1 cut(s) 864
BsrI ACTGG 3 cut(s) 63, 123, 415
BssECI CCNNGG 1 cut(s) 214
BssMI GATC 4 cut(s) 140, 496, 814, 976
Bst2UI CCWGG 3 cut(s) 216, 228, 612
Bst4CI ACNGT 2 cut(s) 535, 547
Bst6I CTCTTC 2 cut(s) 447, 588
BstC8I GCNNGC 6 cut(s) 28, 335, 653, 786, 916, 969
BstDEI CTNAG 4 cut(s) 206, 282, 291, 989
BstEII GGTNACC 2 cut(s) 472, 873
BstF5I GGATG 2 cut(s) 511, 952
BstFNI CGCG 1 cut(s) 333
BstHHI GCGC 1 cut(s) 135
BstKTI GATC 4 cut(s) 143, 499, 817, 979
BstMAI GTCTC 1 cut(s) 192
BstMBI GATC 4 cut(s) 140, 496, 814, 976
BstMWI GCNNNNNNNGC 1 cut(s) 849
BstNI CCWGG 3 cut(s) 216, 228, 612
BstPI GGTNACC 2 cut(s) 472, 873
BstSCI CCNGG 3 cut(s) 214, 226, 610
BstUI CGCG 1 cut(s) 333
BstV1I GCAGC 3 cut(s) 188, 224, 768
BstV2I GAAGAC 1 cut(s) 536
BsuRI GGCC 1 cut(s) 630
BtgZI GCGATG 1 cut(s) 591
BtsCI GGATG 2 cut(s) 511, 952
BtsI GCAGTG 1 cut(s) 627
BtsIMutI CAGTG 2 cut(s) 255, 627
Cac8I GCNNGC 6 cut(s) 28, 335, 653, 786, 916, 969
CaiI CAGNNNCTG 2 cut(s) 149, 626
CfoI GCGC 1 cut(s) 135
Csp6I GTAC 1 cut(s) 573
CviAII CATG 1 cut(s) 870
CviQI GTAC 1 cut(s) 573
DdeI CTNAG 4 cut(s) 206, 282, 291, 989
DpnI GATC 4 cut(s) 142, 498, 816, 978
DpnII GATC 4 cut(s) 140, 496, 814, 976
Eam1104I CTCTTC 2 cut(s) 447, 588
EarI CTCTTC 2 cut(s) 447, 588
Ecl136II GAGCTC 1 cut(s) 1016
Eco24I GRGCYC 1 cut(s) 1018
Eco53kI GAGCTC 1 cut(s) 1016
Eco57I CTGAAG 1 cut(s) 365
Eco88I CYCGRG 1 cut(s) 1011
Eco91I GGTNACC 2 cut(s) 472, 873
EcoICRI GAGCTC 1 cut(s) 1016
EcoO65I GGTNACC 2 cut(s) 472, 873
EcoRII CCWGG 3 cut(s) 214, 226, 610
EcoT38I GRGCYC 1 cut(s) 1018
FaeI CATG 1 cut(s) 873
FalI AAGNNNNNCTT 4 cut(s) 299, 331, 845, 877
FaqI GGGAC 1 cut(s) 197
FatI CATG 1 cut(s) 869
FauI CCCGC 1 cut(s) 326
FblI GTMKAC 2 cut(s) 325, 549
Fnu4HI GCNGC 5 cut(s) 177, 238, 603, 782, 799
FokI GGATG 2 cut(s) 518, 959
FriOI GRGCYC 1 cut(s) 1018
Fsp4HI GCNGC 5 cut(s) 177, 238, 603, 782, 799
FspBI CTAG 2 cut(s) 632, 915
GlaI GCGC 1 cut(s) 134
GluI GCNGC 5 cut(s) 177, 238, 603, 782, 799
GsuI CTGGAG 1 cut(s) 171
HaeIII GGCC 1 cut(s) 630
HhaI GCGC 1 cut(s) 135
Hin1II CATG 1 cut(s) 873
Hin6I GCGC 1 cut(s) 133
HinP1I GCGC 1 cut(s) 133
HincII GTYRAC 1 cut(s) 326
HindII GTYRAC 1 cut(s) 326
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 5 cut(s) 36, 146, 210, 554, 930
HphI GGTGA 5 cut(s) 242, 536, 817, 867, 904
Hpy166II GTNNAC 4 cut(s) 197, 326, 550, 986
Hpy188I TCNGA 4 cut(s) 41, 145, 192, 345
Hpy188III TCNNGA 9 cut(s) 100, 150, 426, 446, 500, 706, 818, 934, 1011
Hpy8I GTNNAC 4 cut(s) 197, 326, 550, 986
HpyAV CCTTC 1 cut(s) 103
HpyCH4III ACNGT 2 cut(s) 535, 547
HpyCH4V TGCA 4 cut(s) 620, 651, 869, 967
HpyF10VI GCNNNNNNNGC 1 cut(s) 849
HpyF3I CTNAG 4 cut(s) 206, 282, 291, 989
Hsp92II CATG 1 cut(s) 873
HspAI GCGC 1 cut(s) 133
Kzo9I GATC 4 cut(s) 140, 496, 814, 976
LmnI GCTCC 3 cut(s) 152, 667, 923
Lsp1109I GCAGC 3 cut(s) 188, 224, 768
LweI GCATC 2 cut(s) 419, 614
MaeI CTAG 2 cut(s) 632, 915
MaeIII GTNAC 3 cut(s) 472, 596, 873
MalI GATC 4 cut(s) 142, 498, 816, 978
MboI GATC 4 cut(s) 140, 496, 814, 976
MboII GAAGA 9 cut(s) 115, 118, 358, 373, 434, 541, 575, 853, 913
MhlI GDGCHC 3 cut(s) 51, 157, 1018
MluCI AATT 4 cut(s) 349, 397, 479, 941
MlyI GAGTC 2 cut(s) 219, 563
MnlI CCTC 8 cut(s) 16, 373, 450, 458, 496, 637, 784, 787
MseI TTAA 2 cut(s) 63, 1021
MspR9I CCNGG 3 cut(s) 216, 228, 612
Mva1269I GAATGC 2 cut(s) 415, 951
MvaI CCWGG 3 cut(s) 216, 228, 612
MvnI CGCG 1 cut(s) 333
MwoI GCNNNNNNNGC 1 cut(s) 849
NdeII GATC 4 cut(s) 140, 496, 814, 976
NheI GCTAGC 1 cut(s) 914
NlaIII CATG 1 cut(s) 873
NmuCI GTSAC 1 cut(s) 873
PcsI WCGNNNNNNNCGW 1 cut(s) 759
PctI GAATGC 2 cut(s) 415, 951
PfeI GAWTC 3 cut(s) 36, 146, 930
PkrI GCNGC 5 cut(s) 178, 239, 604, 783, 800
PleI GAGTC 2 cut(s) 218, 562
PpsI GAGTC 2 cut(s) 218, 562
PsiI TTATAA 2 cut(s) 396, 1004
Psp124BI GAGCTC 1 cut(s) 1018
Psp6I CCWGG 3 cut(s) 214, 226, 610
PspEI GGTNACC 2 cut(s) 472, 873
PspGI CCWGG 3 cut(s) 214, 226, 610
PstNI CAGNNNCTG 2 cut(s) 149, 626
RsaI GTAC 1 cut(s) 574
RsaNI GTAC 1 cut(s) 573
SacI GAGCTC 1 cut(s) 1018
SalI GTCGAC 1 cut(s) 324
SaqAI TTAA 2 cut(s) 63, 1021
SatI GCNGC 5 cut(s) 177, 238, 603, 782, 799
Sau3AI GATC 4 cut(s) 140, 496, 814, 976
SchI GAGTC 2 cut(s) 219, 563
ScrFI CCNGG 3 cut(s) 216, 228, 612
SduI GDGCHC 3 cut(s) 51, 157, 1018
SfaNI GCATC 2 cut(s) 419, 614
SmlI CTYRAG 1 cut(s) 500
SmoI CTYRAG 1 cut(s) 500
Sse9I AATT 4 cut(s) 349, 397, 479, 941
SsiI CCGC 4 cut(s) 333, 603, 788, 799
SspMI CTAG 2 cut(s) 632, 915
SstI GAGCTC 1 cut(s) 1018
StyD4I CCNGG 3 cut(s) 214, 226, 610
TaaI ACNGT 2 cut(s) 535, 547
TaqI TCGA 2 cut(s) 325, 425
TasI AATT 4 cut(s) 349, 397, 479, 941
TauI GCSGC 2 cut(s) 605, 801
TfiI GAWTC 3 cut(s) 36, 146, 930
Tru1I TTAA 2 cut(s) 63, 1021
Tru9I TTAA 2 cut(s) 63, 1021
TscAI CASTG 2 cut(s) 262, 627
TseFI GTSAC 1 cut(s) 873
TseI GCWGC 3 cut(s) 176, 237, 781
Tsp45I GTSAC 1 cut(s) 873
TspDTI ATGAA 7 cut(s) 232, 236, 471, 508, 685, 853, 914
TspRI CASTG 2 cut(s) 262, 627
XapI RAATTY 1 cut(s) 479
XmiI GTMKAC 2 cut(s) 325, 549
XspI CTAG 2 cut(s) 632, 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.