FvH4_7g29542

Actin depolymerisation factor/cofilin -like domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0008202
Physical Location & Seq
Reverse (-)
21704372 .. 21706690
2319 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g29542.t2

Sequence Viewer

Length: 834 bp
ATGGCGAACAAAAGACAGAGAGAGGCTCGCAAGAGATTCAGAGCAGAGCACCCGGAACTGGTCAAAACCCCAGAGCCAACTCCCCCAAAAGACCCAACCAAGAAGAAGAAGAACAAGGTCAAGAAGCCAAGCTTCAAGCCCAAGAGATCAGATTCTGATGGAGCAGCCAAGAAGTCTCGGAGTAAACACCCACTTAGAGTCCCTGGTATGAAGCCCGGAGATAGCTGCTTCATTTGTAAAGGCACTGACCATATTGCCAAGCTTTGCCCTGAGAAAGCTGAGTGGGATAGGCACAAGATATGCTTGTTTTGTCGACAAAGGGGGCATAGTCTGAAGAATTGTCAAAAGAAGAATGAGAATGATATGGAGGCGAAGTTGTGTTATAATTGTGGAGAATCTGGGCATTCGCTTTCTAGATGCCCCTTGCCTCTTCAAGATGGTGGAACTAAATTTGCCAAGTGCTTCGTCTGTAATGAGACTGGTCACCTGAGCAAGGACTGTCCTAAAAACTCTCATGGGATTTATCCAAAGGGCGGTTCTTGTAAAATATGTGGAGGTGTAACACATTTGGCACGAGACTGTCCTAATAAAGACAGCAGGAATTCAATGGGTGGTGGCAATTCTAATCCCAAATGGAATGAACCAAGAGGAAAGCTTACTAAATTTACAAGTGGAGATGATCTTGAGGATGATTTCAGTTTCATAGGTGAGAAGTCTGTTGCCAAAACAGAAGATGATTCTGCTGTAGTCGCTGGTTCTGCTTCTCGTCCACAGGAAGCTTCTGTAACATCAAAAAAGAAACAGGGTCCAAAGATAGTGAATTTTGTTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF zf-CCHC zf-CCHC_4

Protein Analysis

278

Amino Acids

30.54

Weight (kDa)

9.59

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC PF00098 153 - 169 2.2e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 384
AccI GTMKAC 1 cut(s) 313
AciI CCGC 1 cut(s) 534
AcsI RAATTY 4 cut(s) 449, 601, 662, 820
AcuI CTGAAG 1 cut(s) 353
AfiI CCNNNNNNNGG 3 cut(s) 58, 493, 533
AgsI TTSAA 3 cut(s) 136, 434, 606
AjnI CCWGG 1 cut(s) 202
AjuI GAANNNNNNNTTGG 2 cut(s) 520, 552
AluBI AGCT 6 cut(s) 132, 225, 262, 278, 655, 779
AluI AGCT 6 cut(s) 132, 225, 262, 278, 655, 779
Alw21I GWGCWC 1 cut(s) 51
Alw26I GTCTC 3 cut(s) 180, 470, 570
AlwNI CAGNNNCTG 1 cut(s) 155
ApeKI GCWGC 2 cut(s) 164, 225
ApoI RAATTY 4 cut(s) 449, 601, 662, 820
AspS9I GGNCC 1 cut(s) 806
AsuC2I CCSGG 2 cut(s) 53, 216
AsuHPI GGTGA 2 cut(s) 476, 719
AvaII GGWCC 1 cut(s) 806
BauI CACGAG 1 cut(s) 573
Bbv12I GWGCWC 1 cut(s) 51
BbvI GCAGC 2 cut(s) 176, 212
BccI CCATC 2 cut(s) 152, 431
BciT130I CCWGG 1 cut(s) 204
BcnI CCSGG 2 cut(s) 53, 216
BcoDI GTCTC 3 cut(s) 180, 470, 570
BfaI CTAG 1 cut(s) 414
BfmI CTRYAG 1 cut(s) 744
BisI GCNGC 2 cut(s) 165, 226
BlsI GCNGC 2 cut(s) 166, 227
Bme1390I CCNGG 3 cut(s) 53, 204, 216
Bme18I GGWCC 1 cut(s) 806
BmgT120I GGNCC 1 cut(s) 806
BmiI GGNNCC 1 cut(s) 807
BmrFI CCNGG 3 cut(s) 53, 204, 216
BmsI GCATC 1 cut(s) 407
BplI GAGNNNNNCTC 2 cut(s) 10, 42
Bpu10I CCTNAGC 1 cut(s) 488
BpuEI CTTGAG 1 cut(s) 704
BpuMI CCSGG 2 cut(s) 53, 216
BsaJI CCNNGG 1 cut(s) 202
BsaXI ACNNNNNCTCC 2 cut(s) 359, 389
Bsc4I CCNNNNNNNGG 3 cut(s) 58, 493, 533
Bse1I ACTGG 2 cut(s) 63, 484
BseBI CCWGG 1 cut(s) 204
BseDI CCNNGG 1 cut(s) 202
BseGI GGATG 1 cut(s) 694
BseLI CCNNNNNNNGG 3 cut(s) 58, 493, 533
BseMII CTCAG 3 cut(s) 261, 270, 479
BseNI ACTGG 2 cut(s) 63, 484
BseXI GCAGC 2 cut(s) 176, 212
BsiHKAI GWGCWC 1 cut(s) 51
BsiSI CCGG 2 cut(s) 53, 216
BslFI GGGAC 1 cut(s) 185
BslI CCNNNNNNNGG 3 cut(s) 58, 493, 533
BsmAI GTCTC 3 cut(s) 180, 470, 570
BsmFI GGGAC 1 cut(s) 185
BsmI GAATGC 1 cut(s) 403
Bsp1286I GDGCHC 1 cut(s) 51
Bsp143I GATC 2 cut(s) 146, 679
BspACI CCGC 1 cut(s) 534
BspCNI CTCAG 3 cut(s) 262, 271, 480
BspLI GGNNCC 1 cut(s) 807
BsrI ACTGG 2 cut(s) 63, 484
BssECI CCNNGG 1 cut(s) 202
BssMI GATC 2 cut(s) 146, 679
BssSI CACGAG 1 cut(s) 573
Bst2BI CACGAG 1 cut(s) 573
Bst2UI CCWGG 1 cut(s) 204
Bst4CI ACNGT 2 cut(s) 500, 581
Bst6I CTCTTC 1 cut(s) 435
BstC8I GCNNGC 1 cut(s) 28
BstDEI CTNAG 4 cut(s) 194, 270, 279, 488
BstEII GGTNACC 1 cut(s) 482
BstENI CCTNNNNNAGG 1 cut(s) 491
BstF5I GGATG 1 cut(s) 694
BstKTI GATC 2 cut(s) 149, 682
BstMAI GTCTC 3 cut(s) 180, 470, 570
BstMBI GATC 2 cut(s) 146, 679
BstMWI GCNNNNNNNGC 2 cut(s) 749, 758
BstNI CCWGG 1 cut(s) 204
BstPI GGTNACC 1 cut(s) 482
BstSCI CCNGG 3 cut(s) 51, 202, 214
BstSFI CTRYAG 1 cut(s) 744
BstV1I GCAGC 2 cut(s) 176, 212
BtsCI GGATG 1 cut(s) 694
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 1 cut(s) 28
CaiI CAGNNNCTG 1 cut(s) 155
Cfr13I GGNCC 1 cut(s) 806
CviAII CATG 1 cut(s) 515
DdeI CTNAG 4 cut(s) 194, 270, 279, 488
DpnI GATC 2 cut(s) 148, 681
DpnII GATC 2 cut(s) 146, 679
Eam1104I CTCTTC 1 cut(s) 435
EarI CTCTTC 1 cut(s) 435
Eco47I GGWCC 1 cut(s) 806
Eco57I CTGAAG 1 cut(s) 353
Eco91I GGTNACC 1 cut(s) 482
EcoNI CCTNNNNNAGG 1 cut(s) 491
EcoO65I GGTNACC 1 cut(s) 482
EcoRI GAATTC 1 cut(s) 601
EcoRII CCWGG 1 cut(s) 202
FaeI CATG 1 cut(s) 518
FaiI YATR 9 cut(s) 209, 252, 301, 327, 365, 384, 516, 550, 704
FalI AAGNNNNNCTT 4 cut(s) 116, 148, 287, 319
FaqI GGGAC 1 cut(s) 185
FatI CATG 1 cut(s) 514
FblI GTMKAC 1 cut(s) 313
Fnu4HI GCNGC 2 cut(s) 165, 226
FokI GGATG 1 cut(s) 701
Fsp4HI GCNGC 2 cut(s) 165, 226
FspBI CTAG 1 cut(s) 414
GluI GCNGC 2 cut(s) 165, 226
HapII CCGG 2 cut(s) 53, 216
Hin1II CATG 1 cut(s) 518
HincII GTYRAC 1 cut(s) 314
HindII GTYRAC 1 cut(s) 314
HindIII AAGCTT 4 cut(s) 130, 260, 653, 777
HinfI GANTC 5 cut(s) 36, 152, 198, 395, 737
HpaII CCGG 2 cut(s) 53, 216
HphI GGTGA 2 cut(s) 476, 719
Hpy166II GTNNAC 3 cut(s) 185, 314, 770
Hpy188I TCNGA 5 cut(s) 41, 151, 157, 180, 333
Hpy188III TCNNGA 4 cut(s) 121, 414, 434, 683
Hpy8I GTNNAC 3 cut(s) 185, 314, 770
HpyCH4III ACNGT 2 cut(s) 500, 581
HpyF10VI GCNNNNNNNGC 2 cut(s) 749, 758
HpyF3I CTNAG 4 cut(s) 194, 270, 279, 488
Hsp92II CATG 1 cut(s) 518
Kzo9I GATC 2 cut(s) 146, 679
LmnI GCTCC 1 cut(s) 161
Lsp1109I GCAGC 2 cut(s) 176, 212
LweI GCATC 1 cut(s) 407
MaeI CTAG 1 cut(s) 414
MaeIII GTNAC 3 cut(s) 482, 559, 784
MalI GATC 2 cut(s) 148, 681
MboI GATC 2 cut(s) 146, 679
MboII GAAGA 7 cut(s) 115, 118, 121, 346, 361, 422, 743
MhlI GDGCHC 1 cut(s) 51
MluCI AATT 7 cut(s) 337, 385, 449, 601, 619, 662, 820
MlyI GAGTC 1 cut(s) 207
MmeI TCCRAC 1 cut(s) 808
MnlI CCTC 6 cut(s) 16, 361, 438, 548, 641, 679
MspI CCGG 2 cut(s) 53, 216
MspR9I CCNGG 3 cut(s) 53, 204, 216
Mva1269I GAATGC 1 cut(s) 403
MvaI CCWGG 1 cut(s) 204
MwoI GCNNNNNNNGC 2 cut(s) 749, 758
NciI CCSGG 2 cut(s) 53, 216
NdeII GATC 2 cut(s) 146, 679
NlaIII CATG 1 cut(s) 518
NlaIV GGNNCC 1 cut(s) 807
NmuCI GTSAC 1 cut(s) 482
PctI GAATGC 1 cut(s) 403
PfeI GAWTC 4 cut(s) 36, 152, 395, 737
PkrI GCNGC 2 cut(s) 166, 227
PleI GAGTC 1 cut(s) 206
PpsI GAGTC 1 cut(s) 206
PsiI TTATAA 1 cut(s) 384
Psp6I CCWGG 1 cut(s) 202
PspEI GGTNACC 1 cut(s) 482
PspGI CCWGG 1 cut(s) 202
PspN4I GGNNCC 1 cut(s) 807
PspPI GGNCC 1 cut(s) 806
PstNI CAGNNNCTG 1 cut(s) 155
SalI GTCGAC 1 cut(s) 312
SatI GCNGC 2 cut(s) 165, 226
Sau3AI GATC 2 cut(s) 146, 679
Sau96I GGNCC 1 cut(s) 806
SchI GAGTC 1 cut(s) 207
ScrFI CCNGG 3 cut(s) 53, 204, 216
SduI GDGCHC 1 cut(s) 51
SfaNI GCATC 1 cut(s) 407
SfcI CTRYAG 1 cut(s) 744
SinI GGWCC 1 cut(s) 806
SmlI CTYRAG 1 cut(s) 683
SmoI CTYRAG 1 cut(s) 683
Sse9I AATT 7 cut(s) 337, 385, 449, 601, 619, 662, 820
SsiI CCGC 1 cut(s) 534
SspMI CTAG 1 cut(s) 414
StyD4I CCNGG 3 cut(s) 51, 202, 214
TaaI ACNGT 2 cut(s) 500, 581
TaqI TCGA 1 cut(s) 313
TasI AATT 7 cut(s) 337, 385, 449, 601, 619, 662, 820
TfiI GAWTC 4 cut(s) 36, 152, 395, 737
TscAI CASTG 1 cut(s) 250
TseFI GTSAC 1 cut(s) 482
TseI GCWGC 2 cut(s) 164, 225
Tsp45I GTSAC 1 cut(s) 482
TspDTI ATGAA 4 cut(s) 220, 224, 654, 691
TspRI CASTG 1 cut(s) 250
VpaK11BI GGWCC 1 cut(s) 806
XagI CCTNNNNNAGG 1 cut(s) 491
XapI RAATTY 4 cut(s) 449, 601, 662, 820
XbaI TCTAGA 1 cut(s) 413
XmiI GTMKAC 1 cut(s) 313
XspI CTAG 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.