Rh1AG421100

Actin depolymerisation factor/cofilin -like domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0008202
Physical Location & Seq
Reverse (-)
65115070 .. 65116014
945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG421100.1

Sequence Viewer

Length: 426 bp
ATGGCCGCCTCGCCTGGCTTTGCAGTGTCTGGCCTAGCCGTGAATGAGGAGTGCAAGCAGAAGTTCTTGGAGCTAAAAGCCAAGAGAAACCATCGTTTCATTATATTCAAGATTGATAAGCAAGAAGTGGTGGTAGAGAAACTTGGAGAGCCAGACGAAACTTACGATGATTTCACAGCCTCGCTGCCTGCGGAGGAGTGTCGCTATGCTGTGTTTGATCTTGATTTCACTACTATTGAGAACTGCCAGAAAAGCAAGATTTTCTTCATTGCATGGTCACCTGATACATCAAAGGTGAGAATGAAGATGGTGTATGCTAGCTCCAAAGATAGATTCAAGAGAGAATTGGATGGCATTCAAGTTGAGTTGCAAGCAACTGATCCAAGTGAATTGAGCTTTGACATTATAAAATCCCGAGCTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF zf-CCHC zf-CCHC_4

Protein Analysis

141

Amino Acids

16.14

Weight (kDa)

5.3

Isoelectric Point (pI)

48.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 12 - 139 4e-43 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 407
AciI CCGC 2 cut(s) 6, 191
AclWI GGATC 1 cut(s) 374
AcoI YGGCCR 1 cut(s) 3
AgsI TTSAA 3 cut(s) 109, 337, 359
AjnI CCWGG 1 cut(s) 13
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AluBI AGCT 4 cut(s) 73, 321, 396, 419
AluI AGCT 4 cut(s) 73, 321, 396, 419
Alw21I GWGCWC 1 cut(s) 421
AlwI GGATC 1 cut(s) 374
AlwNI CAGNNNCTG 1 cut(s) 29
Ama87I CYCGRG 1 cut(s) 414
AoxI GGCC 2 cut(s) 3, 31
ApeKI GCWGC 1 cut(s) 184
AsuHPI GGTGA 2 cut(s) 270, 307
AsuNHI GCTAGC 1 cut(s) 317
AvaI CYCGRG 1 cut(s) 414
BanII GRGCYC 1 cut(s) 421
Bbv12I GWGCWC 1 cut(s) 421
BbvI GCAGC 1 cut(s) 171
BccI CCATC 3 cut(s) 99, 301, 344
BceAI ACGGC 1 cut(s) 23
BciT130I CCWGG 1 cut(s) 15
BfaI CTAG 2 cut(s) 35, 318
BisI GCNGC 2 cut(s) 6, 185
BlsI GCNGC 2 cut(s) 7, 186
Bme1390I CCNGG 1 cut(s) 15
BmeT110I CYCGRG 1 cut(s) 414
BmrFI CCNGG 1 cut(s) 15
BmtI GCTAGC 1 cut(s) 321
Bse3DI GCAATG 1 cut(s) 267
BseBI CCWGG 1 cut(s) 15
BseGI GGATG 1 cut(s) 355
BseMI GCAATG 1 cut(s) 267
BseRI GAGGAG 2 cut(s) 62, 209
BseXI GCAGC 1 cut(s) 171
BshFI GGCC 2 cut(s) 5, 33
BsiHKAI GWGCWC 1 cut(s) 421
BsiHKCI CYCGRG 1 cut(s) 414
BsmI GAATGC 1 cut(s) 354
BsnI GGCC 2 cut(s) 5, 33
BsoBI CYCGRG 1 cut(s) 414
Bsp1286I GDGCHC 1 cut(s) 421
Bsp143I GATC 2 cut(s) 217, 379
BspACI CCGC 2 cut(s) 6, 191
BspANI GGCC 2 cut(s) 5, 33
BspOI GCTAGC 1 cut(s) 321
BspPI GGATC 1 cut(s) 374
BsrDI GCAATG 1 cut(s) 267
BssMI GATC 2 cut(s) 217, 379
Bst2UI CCWGG 1 cut(s) 15
BstC8I GCNNGC 4 cut(s) 56, 189, 319, 372
BstEII GGTNACC 1 cut(s) 276
BstF5I GGATG 1 cut(s) 355
BstKTI GATC 2 cut(s) 220, 382
BstMBI GATC 2 cut(s) 217, 379
BstMWI GCNNNNNNNGC 1 cut(s) 252
BstNI CCWGG 1 cut(s) 15
BstPI GGTNACC 1 cut(s) 276
BstSCI CCNGG 1 cut(s) 13
BstV1I GCAGC 1 cut(s) 171
BsuRI GGCC 2 cut(s) 5, 33
BtsCI GGATG 1 cut(s) 355
BtsI GCAGTG 1 cut(s) 30
BtsIMutI CAGTG 1 cut(s) 30
Cac8I GCNNGC 4 cut(s) 56, 189, 319, 372
CaiI CAGNNNCTG 1 cut(s) 29
CviAII CATG 1 cut(s) 273
DpnI GATC 2 cut(s) 219, 381
DpnII GATC 2 cut(s) 217, 379
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 419
Eco24I GRGCYC 1 cut(s) 421
Eco53kI GAGCTC 1 cut(s) 419
Eco88I CYCGRG 1 cut(s) 414
Eco91I GGTNACC 1 cut(s) 276
EcoICRI GAGCTC 1 cut(s) 419
EcoO65I GGTNACC 1 cut(s) 276
EcoRII CCWGG 1 cut(s) 13
EcoT38I GRGCYC 1 cut(s) 421
FaeI CATG 1 cut(s) 276
FaiI YATR 5 cut(s) 104, 207, 274, 315, 407
FalI AAGNNNNNCTT 2 cut(s) 248, 280
FatI CATG 1 cut(s) 272
Fnu4HI GCNGC 2 cut(s) 6, 185
FokI GGATG 1 cut(s) 362
FriOI GRGCYC 1 cut(s) 421
Fsp4HI GCNGC 2 cut(s) 6, 185
FspBI CTAG 2 cut(s) 35, 318
GluI GCNGC 2 cut(s) 6, 185
HaeIII GGCC 2 cut(s) 5, 33
Hin1II CATG 1 cut(s) 276
HinfI GANTC 1 cut(s) 333
HphI GGTGA 2 cut(s) 270, 307
Hpy188III TCNNGA 4 cut(s) 109, 221, 337, 414
HpyCH4V TGCA 4 cut(s) 23, 54, 272, 370
HpyF10VI GCNNNNNNNGC 1 cut(s) 252
Hsp92II CATG 1 cut(s) 276
Kzo9I GATC 2 cut(s) 217, 379
LmnI GCTCC 2 cut(s) 70, 326
LpnPI CCDG 6 cut(s) 15, 27, 165, 201, 260, 294
Lsp1109I GCAGC 1 cut(s) 171
MaeI CTAG 2 cut(s) 35, 318
MaeIII GTNAC 1 cut(s) 276
MalI GATC 2 cut(s) 219, 381
MboI GATC 2 cut(s) 217, 379
MboII GAAGA 2 cut(s) 256, 316
MhlI GDGCHC 1 cut(s) 421
MluCI AATT 2 cut(s) 344, 389
MnlI CCTC 4 cut(s) 19, 40, 187, 190
MseI TTAA 1 cut(s) 424
MspR9I CCNGG 1 cut(s) 15
Mva1269I GAATGC 1 cut(s) 354
MvaI CCWGG 1 cut(s) 15
MwoI GCNNNNNNNGC 1 cut(s) 252
NdeII GATC 2 cut(s) 217, 379
NheI GCTAGC 1 cut(s) 317
NlaIII CATG 1 cut(s) 276
NmuCI GTSAC 1 cut(s) 276
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PctI GAATGC 1 cut(s) 354
PfeI GAWTC 1 cut(s) 333
PkrI GCNGC 2 cut(s) 7, 186
PsiI TTATAA 1 cut(s) 407
Psp124BI GAGCTC 1 cut(s) 421
Psp6I CCWGG 1 cut(s) 13
PspEI GGTNACC 1 cut(s) 276
PspGI CCWGG 1 cut(s) 13
PstNI CAGNNNCTG 1 cut(s) 29
SacI GAGCTC 1 cut(s) 421
SaqAI TTAA 1 cut(s) 424
SatI GCNGC 2 cut(s) 6, 185
Sau3AI GATC 2 cut(s) 217, 379
ScrFI CCNGG 1 cut(s) 15
SduI GDGCHC 1 cut(s) 421
SetI ASST 6 cut(s) 75, 283, 297, 323, 398, 421
Sse9I AATT 2 cut(s) 344, 389
SsiI CCGC 2 cut(s) 6, 191
SspMI CTAG 2 cut(s) 35, 318
SstI GAGCTC 1 cut(s) 421
StyD4I CCNGG 1 cut(s) 13
TasI AATT 2 cut(s) 344, 389
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 1 cut(s) 333
Tru1I TTAA 1 cut(s) 424
Tru9I TTAA 1 cut(s) 424
TscAI CASTG 1 cut(s) 30
TseFI GTSAC 1 cut(s) 276
TseI GCWGC 1 cut(s) 184
Tsp45I GTSAC 1 cut(s) 276
TspDTI ATGAA 3 cut(s) 88, 256, 317
TspRI CASTG 1 cut(s) 30
XspI CTAG 2 cut(s) 35, 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.