Rh1DG410900

Actin depolymerisation factor/cofilin -like domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0008202
Physical Location & Seq
Reverse (-)
62790651 .. 62793398
2748 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG410900.1

Sequence Viewer

Length: 795 bp
ATGAGAATGATACGGAGGCGAAGTTATGTTATAATTGTGGGGAAACCGGGCATTCGCTTTCTAGATGCCCCTTACCTCTTCAAGATGGGTGGTTCTTGTAAAATATGTGGCGGTGTAACACATTTGGCACGAGACTGTCCTAACAAAGACAGCAGGAATTCAATAGGTTCTGGCAATTCTAATCCCAAATGGAATGAACCAAGAGGAAAGGTTACCAAATTTGCGAGTGGAGATGATCTTGAGGATGATTTCAGTTTCATAGGTGAGAAGACTGTTGCCAAAACAGTAGACGAGTCTGCCAATGTAGTTGGTGCTGCTTCTCTTCCACAAGAAAGTAACGTAAAATCAAAGAAGAAACAGGGTCCCAAAATAGCCGCCTCGCCTGGCTTTGCAGTGTCTGGCCTAGCCGTGAATGAGGAGTGCAAGCAGAAGTTCTTGGAGCTAAAAGCCAAGAGAAACCATCGTTTCATTATATTCAAGATTGATAAGCAAGAAGTGGTGGTAGAGAAACTTGGAGAGCCAGACGAAACATACGATGATTTCACAGCCTCCCTGCCTGCGGAGGAGTGCCGCTATGCTGTGTTTGATCTTGATTTCACCACTATTGAGAACTGCCAGAAAAGCAAGATTTTCTTCATTGCATGGTCACCTGATACATCAAAGGTGAGAATGAAGATGGTGTATGCTAGCTCCAAAGATAGATTCAAGAGAGAATTGGATGGCATTCAAGTTGAGTTGCAAGCAACTGATCCAAGTGAATTGAGCTTTGACATTATAAAATCCCGAGCTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF zf-CCHC zf-CCHC_4

Protein Analysis

264

Amino Acids

29.5

Weight (kDa)

8.89

Isoelectric Point (pI)

43.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 135 - 262 2.8e-42 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 32, 776
AccI GTMKAC 1 cut(s) 288
AciI CCGC 4 cut(s) 111, 375, 560, 571
AclWI GGATC 1 cut(s) 743
AcsI RAATTY 2 cut(s) 157, 218
AfiI CCNNNNNNNGG 1 cut(s) 559
AgsI TTSAA 5 cut(s) 82, 162, 478, 706, 728
AjnI CCWGG 1 cut(s) 382
AjuI GAANNNNNNNTTGG 2 cut(s) 686, 718
AluBI AGCT 4 cut(s) 442, 690, 765, 788
AluI AGCT 4 cut(s) 442, 690, 765, 788
Alw21I GWGCWC 1 cut(s) 790
Alw26I GTCTC 1 cut(s) 126
AlwI GGATC 1 cut(s) 743
AlwNI CAGNNNCTG 1 cut(s) 398
Ama87I CYCGRG 1 cut(s) 783
AoxI GGCC 1 cut(s) 400
ApeKI GCWGC 1 cut(s) 314
ApoI RAATTY 2 cut(s) 157, 218
AspS9I GGNCC 1 cut(s) 362
AsuC2I CCSGG 1 cut(s) 48
AsuHPI GGTGA 4 cut(s) 275, 589, 639, 676
AsuNHI GCTAGC 1 cut(s) 686
AvaI CYCGRG 1 cut(s) 783
AvaII GGWCC 1 cut(s) 362
BanII GRGCYC 1 cut(s) 790
BauI CACGAG 1 cut(s) 129
BbsI GAAGAC 1 cut(s) 275
Bbv12I GWGCWC 1 cut(s) 790
BbvI GCAGC 1 cut(s) 301
BccI CCATC 4 cut(s) 79, 468, 670, 713
BceAI ACGGC 1 cut(s) 392
BciT130I CCWGG 1 cut(s) 384
BcnI CCSGG 1 cut(s) 48
BcoDI GTCTC 1 cut(s) 126
BfaI CTAG 3 cut(s) 62, 404, 687
BisI GCNGC 3 cut(s) 315, 375, 571
BlsI GCNGC 3 cut(s) 316, 376, 572
Bme1390I CCNGG 2 cut(s) 48, 384
Bme18I GGWCC 1 cut(s) 362
BmeT110I CYCGRG 1 cut(s) 783
BmgT120I GGNCC 1 cut(s) 362
BmiI GGNNCC 2 cut(s) 363, 364
BmrFI CCNGG 2 cut(s) 48, 384
BmsI GCATC 1 cut(s) 55
BmtI GCTAGC 1 cut(s) 690
BpiI GAAGAC 1 cut(s) 275
BpuEI CTTGAG 1 cut(s) 260
BpuMI CCSGG 1 cut(s) 48
BsaXI ACNNNNNCTCC 2 cut(s) 7, 37
Bsc4I CCNNNNNNNGG 1 cut(s) 559
Bse3DI GCAATG 1 cut(s) 636
BseBI CCWGG 1 cut(s) 384
BseGI GGATG 2 cut(s) 250, 724
BseLI CCNNNNNNNGG 1 cut(s) 559
BseMI GCAATG 1 cut(s) 636
BseRI GAGGAG 2 cut(s) 431, 578
BseXI GCAGC 1 cut(s) 301
BshFI GGCC 1 cut(s) 402
BsiHKAI GWGCWC 1 cut(s) 790
BsiHKCI CYCGRG 1 cut(s) 783
BsiSI CCGG 1 cut(s) 47
BslFI GGGAC 1 cut(s) 348
BslI CCNNNNNNNGG 1 cut(s) 559
BsmAI GTCTC 1 cut(s) 126
BsmFI GGGAC 1 cut(s) 348
BsmI GAATGC 2 cut(s) 51, 723
BsnI GGCC 1 cut(s) 402
BsoBI CYCGRG 1 cut(s) 783
Bsp1286I GDGCHC 1 cut(s) 790
Bsp143I GATC 3 cut(s) 235, 586, 748
BspACI CCGC 4 cut(s) 111, 375, 560, 571
BspANI GGCC 1 cut(s) 402
BspLI GGNNCC 2 cut(s) 363, 364
BspOI GCTAGC 1 cut(s) 690
BspPI GGATC 1 cut(s) 743
BsrDI GCAATG 1 cut(s) 636
BssMI GATC 3 cut(s) 235, 586, 748
BssSI CACGAG 1 cut(s) 129
Bst2BI CACGAG 1 cut(s) 129
Bst2UI CCWGG 1 cut(s) 384
Bst4CI ACNGT 3 cut(s) 137, 274, 286
Bst6I CTCTTC 2 cut(s) 83, 327
BstC8I GCNNGC 4 cut(s) 425, 558, 688, 741
BstEII GGTNACC 2 cut(s) 211, 645
BstF5I GGATG 2 cut(s) 250, 724
BstKTI GATC 3 cut(s) 238, 589, 751
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 3 cut(s) 235, 586, 748
BstMWI GCNNNNNNNGC 1 cut(s) 621
BstNI CCWGG 1 cut(s) 384
BstPI GGTNACC 2 cut(s) 211, 645
BstSCI CCNGG 2 cut(s) 46, 382
BstV1I GCAGC 1 cut(s) 301
BstV2I GAAGAC 1 cut(s) 275
BsuRI GGCC 1 cut(s) 402
BtsCI GGATG 2 cut(s) 250, 724
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 1 cut(s) 399
Cac8I GCNNGC 4 cut(s) 425, 558, 688, 741
CaiI CAGNNNCTG 1 cut(s) 398
Cfr13I GGNCC 1 cut(s) 362
CspCI CAANNNNNGTGG 2 cut(s) 70, 105
CviAII CATG 1 cut(s) 642
DpnI GATC 3 cut(s) 237, 588, 750
DpnII GATC 3 cut(s) 235, 586, 748
Eam1104I CTCTTC 2 cut(s) 83, 327
EarI CTCTTC 2 cut(s) 83, 327
Ecl136II GAGCTC 1 cut(s) 788
Eco24I GRGCYC 1 cut(s) 790
Eco47I GGWCC 1 cut(s) 362
Eco53kI GAGCTC 1 cut(s) 788
Eco88I CYCGRG 1 cut(s) 783
Eco91I GGTNACC 2 cut(s) 211, 645
EcoICRI GAGCTC 1 cut(s) 788
EcoO109I RGGNCCY 1 cut(s) 362
EcoO65I GGTNACC 2 cut(s) 211, 645
EcoRI GAATTC 1 cut(s) 157
EcoRII CCWGG 1 cut(s) 382
EcoT38I GRGCYC 1 cut(s) 790
FaeI CATG 1 cut(s) 645
FalI AAGNNNNNCTT 2 cut(s) 617, 649
FaqI GGGAC 1 cut(s) 348
FatI CATG 1 cut(s) 641
FblI GTMKAC 1 cut(s) 288
Fnu4HI GCNGC 3 cut(s) 315, 375, 571
FokI GGATG 2 cut(s) 257, 731
FriOI GRGCYC 1 cut(s) 790
Fsp4HI GCNGC 3 cut(s) 315, 375, 571
FspBI CTAG 3 cut(s) 62, 404, 687
GluI GCNGC 3 cut(s) 315, 375, 571
HaeIII GGCC 1 cut(s) 402
HapII CCGG 1 cut(s) 47
Hin1II CATG 1 cut(s) 645
HinfI GANTC 2 cut(s) 293, 702
HpaII CCGG 1 cut(s) 47
HphI GGTGA 4 cut(s) 275, 589, 639, 676
Hpy166II GTNNAC 1 cut(s) 289
Hpy188III TCNNGA 7 cut(s) 62, 82, 239, 478, 590, 706, 783
Hpy8I GTNNAC 1 cut(s) 289
HpyCH4III ACNGT 3 cut(s) 137, 274, 286
HpyCH4IV ACGT 1 cut(s) 339
HpyCH4V TGCA 4 cut(s) 392, 423, 641, 739
HpyF10VI GCNNNNNNNGC 1 cut(s) 621
HpySE526I ACGT 1 cut(s) 339
Hsp92II CATG 1 cut(s) 645
KflI GGGWCCC 1 cut(s) 362
Kzo9I GATC 3 cut(s) 235, 586, 748
LmnI GCTCC 2 cut(s) 439, 695
Lsp1109I GCAGC 1 cut(s) 301
LweI GCATC 1 cut(s) 55
MaeI CTAG 3 cut(s) 62, 404, 687
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 4 cut(s) 115, 211, 335, 645
MalI GATC 3 cut(s) 237, 588, 750
MboI GATC 3 cut(s) 235, 586, 748
MboII GAAGA 6 cut(s) 70, 280, 314, 364, 625, 685
MhlI GDGCHC 1 cut(s) 790
MluCI AATT 6 cut(s) 33, 157, 175, 218, 713, 758
MlyI GAGTC 1 cut(s) 302
MnlI CCTC 8 cut(s) 9, 86, 197, 235, 388, 409, 556, 559
MseI TTAA 1 cut(s) 793
MspI CCGG 1 cut(s) 47
MspR9I CCNGG 2 cut(s) 48, 384
Mva1269I GAATGC 2 cut(s) 51, 723
MvaI CCWGG 1 cut(s) 384
MwoI GCNNNNNNNGC 1 cut(s) 621
NciI CCSGG 1 cut(s) 48
NdeII GATC 3 cut(s) 235, 586, 748
NheI GCTAGC 1 cut(s) 686
NlaIII CATG 1 cut(s) 645
NlaIV GGNNCC 2 cut(s) 363, 364
NmuCI GTSAC 1 cut(s) 645
PcsI WCGNNNNNNNCGW 1 cut(s) 531
PctI GAATGC 2 cut(s) 51, 723
PfeI GAWTC 1 cut(s) 702
PkrI GCNGC 3 cut(s) 316, 376, 572
PleI GAGTC 1 cut(s) 301
PpsI GAGTC 1 cut(s) 301
PpuMI RGGWCCY 1 cut(s) 362
PsiI TTATAA 2 cut(s) 32, 776
Psp124BI GAGCTC 1 cut(s) 790
Psp5II RGGWCCY 1 cut(s) 362
Psp6I CCWGG 1 cut(s) 382
PspEI GGTNACC 2 cut(s) 211, 645
PspGI CCWGG 1 cut(s) 382
PspN4I GGNNCC 2 cut(s) 363, 364
PspPI GGNCC 1 cut(s) 362
PspPPI RGGWCCY 1 cut(s) 362
PstNI CAGNNNCTG 1 cut(s) 398
SacI GAGCTC 1 cut(s) 790
SaqAI TTAA 1 cut(s) 793
SatI GCNGC 3 cut(s) 315, 375, 571
Sau3AI GATC 3 cut(s) 235, 586, 748
Sau96I GGNCC 1 cut(s) 362
SchI GAGTC 1 cut(s) 302
ScrFI CCNGG 2 cut(s) 48, 384
SduI GDGCHC 1 cut(s) 790
SfaNI GCATC 1 cut(s) 55
SinI GGWCC 1 cut(s) 362
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 6 cut(s) 33, 157, 175, 218, 713, 758
SsiI CCGC 4 cut(s) 111, 375, 560, 571
SspMI CTAG 3 cut(s) 62, 404, 687
SstI GAGCTC 1 cut(s) 790
StyD4I CCNGG 2 cut(s) 46, 382
TaaI ACNGT 3 cut(s) 137, 274, 286
TaiI ACGT 1 cut(s) 342
TasI AATT 6 cut(s) 33, 157, 175, 218, 713, 758
TauI GCSGC 2 cut(s) 377, 573
TfiI GAWTC 1 cut(s) 702
Tru1I TTAA 1 cut(s) 793
Tru9I TTAA 1 cut(s) 793
TscAI CASTG 1 cut(s) 399
TseFI GTSAC 1 cut(s) 645
TseI GCWGC 1 cut(s) 314
Tsp45I GTSAC 1 cut(s) 645
TspDTI ATGAA 5 cut(s) 210, 247, 457, 625, 686
TspGWI ACGGA 1 cut(s) 28
TspRI CASTG 1 cut(s) 399
VpaK11BI GGWCC 1 cut(s) 362
XapI RAATTY 2 cut(s) 157, 218
XbaI TCTAGA 1 cut(s) 61
XmiI GTMKAC 1 cut(s) 288
XspI CTAG 3 cut(s) 62, 404, 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.