AT5G55830

Legume lectin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005408
Physical Location & Seq
Forward (+)
22594617 .. 22596843
2227 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G55830.1

Sequence Viewer

Length: 2046 bp
ATGTCTCTATCAAGAAAGCTTCTTGTTATCTTCTTCACATGGATAACAGCTCTATCCATGTCGAAACCAATCTTCGTATCATCCGATAACATGAATTTCACATTCAAATCCTTCACAATCCGTAACCTCACATTCCTCGGAGATTCACATCTCCGAAACGGTGTCGTTGGGCTCACAAGAGAGCTAGGTGTTCCCGATACAAGCTCCGGTACAGTAATCTACAACAATCCGATCCGTTTCTACGATCCGGATTCAAACACCACGGCGTCTTTCTCCACTCACTTCTCCTTCACCGTTCAAAACTTAAACCCAGATCCAACCTCCGCCGGTGACGGACTCGCTTTCTTCCTCTCTCACGACAACGATACTTTAGGAAGTCCCGGTGGTTACTTAGGTCTCGTCAATTCCTCTCAGCCGATGAAGAATCGATTCGTCGCTATCGAATTCGATACGAAATTGGATCCTCATTTCAACGATCCAAATGGTAATCACATTGGTTTAGATGTTGATAGTTTGAACTCAATCTCAACTTCAGATCCTTTGCTTTCGTCTCAGATTGATCTCAAATCTGGTAAATCGATAACTTCTTGGATCGATTACAAGAACGATTTACGTTTATTGAACGTGTTCTTGAGTTATACAGATCCAGTCACTACAACGAAGAAGCCAGAGAAGCCTCTTTTATCGGTTAACATCGATCTTTCTCCGTTCTTGAACGGTGAAATGTATGTAGGATTCTCAGGTTCAACAGAAGGAAGCACAGAGATTCATCTGATCGAGAATTGGAGTTTCAAGACTTCTGGATTTCTTCCGGTGAGATCGAAATCTAATCATCTTCACAATGTTTCAGATAGTTCTGTGGTGAATGATGATCCTGTTGTGATACCGAGTAAGAAGCGGAGGCATCGACACAATCTTGCTATTGGACTTGGTATTTCTTGTCCGGTGTTAATCTGTTTAGCTCTCTTTGTGTTTGGGTACTTTACTTTGAAGAAATGGAAAAGTGTGAAAGCAGAGAAGGAGCTTAAGACTGAGCTAATCACTGGTTTAAGAGAGTTTAGCTATAAAGAGCTTTACACAGCTACTAAAGGGTTTCATTCAAGTAGAGTCATTGGAAGAGGAGCGTTTGGGAATGTTTACAGAGCTATGTTTGTTTCCTCTGGGACGATCTCTGCTGTGAAAAGATCGAGGCATAATTCAACCGAAGGTAAAACAGAGTTTTTGGCAGAGTTATCGATTATTGCTTGCTTGCGGCACAAGAATCTTGTTCAGTTGCAGGGTTGGTGTAACGAGAAAGGTGAATTGCTTCTTGTCTATGAGTTCATGCCTAATGGAAGTCTTGATAAGATTCTGTATCAAGAATCACAAACAGGAGCTGTGGCGCTTGACTGGTCTCATAGGCTGAATATAGCGATTGGTTTAGCATCAGCTTTGTCTTATCTTCACCATGAGTGTGAGCAGCAAGTGGTTCACAGAGATATTAAGACGAGCAACATAATGCTTGACATAAACTTCAACGCAAGGCTGGGAGATTTTGGTCTAGCTAGACTCACGGAGCATGATAAGAGCCCTGTCTCAACTTTAACCGCTGGTACAATGGGTTACCTCGCACCAGAGTATCTCCAATACGGCACAGCGACTGAGAAAACAGATGCTTTTAGCTACGGAGTGGTGATCCTAGAAGTTGCTTGTGGGAGAAGGCCAATAGATAAAGAACCAGAGAGTCAAAAGACAGTGAATCTAGTGGATTGGGTTTGGAGATTGCACAGTGAAGGAAGAGTTCTTGAAGCTGTGGATGAGAGATTGAAAGGTGAGTTTGATGAAGAAATGATGAAGAAACTTTTGCTTGTGGGTCTTAAATGTGCCCATCCAGATAGTAATGAGAGACCATCTATGAGACGTGTTCTTCAGATACTTAACAATGAGATTGAGCCAAGTCCGGTTCCGAAGATGAAACCTACATTGTCTTTCTCTTGTGGGTTAAGTCTTGATGACATTGTTTCGGAAGATGAAGAAGGAGACAGCATCGTGTACGTTGTATCTTAG

Protein Analysis

681

Amino Acids

75.84

Weight (kDa)

6.15

Isoelectric Point (pI)

41.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 31 - 275 4.4e-64 Legume lectin domain
Lectin_L-type_dom PF18483 52 - 258 5.2e-08 Legume lectin beta-barrel domain
Pkinase PF00069 365 - 637 4.4e-50 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 367 - 638 4.1e-47 Protein tyrosine and serine/threonine kinase
Kinase-like PF14531 447 - 576 8.1e-06 Kinase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 247
AciI CCGC 4 cut(s) 324, 898, 1252, 1587
AcsI RAATTY 2 cut(s) 94, 443
AcuI CTGAAG 2 cut(s) 516, 1892
AcyI GRCGYC 1 cut(s) 266
AfaI GTAC 4 cut(s) 211, 980, 1594, 2033
AflII CTTAAG 1 cut(s) 1025
AflIII ACRYGT 2 cut(s) 624, 1900
AjiI CACGTC 1 cut(s) 1901
Alw26I GTCTC 8 cut(s) 9, 401, 555, 1398, 1579, 1879, 1891, 2013
AlwNI CAGNNNCTG 2 cut(s) 1376, 1640
Aor13HI TCCGGA 1 cut(s) 247
AoxI GGCC 1 cut(s) 1700
ApeKI GCWGC 1 cut(s) 1459
ApoI RAATTY 2 cut(s) 94, 443
Asp700I GAANNNNTTC 3 cut(s) 428, 626, 1305
AspLEI GCGC 1 cut(s) 1384
AsuC2I CCSGG 1 cut(s) 381
AsuHPI GGTGA 9 cut(s) 283, 341, 731, 826, 874, 1310, 1436, 1684, 1823
BaeGI GKGCMC 1 cut(s) 1867
BaeI ACNNNNGTAYC 2 cut(s) 1601, 1634
BamHI GGATCC 1 cut(s) 460
BanII GRGCYC 2 cut(s) 174, 1571
BbvI GCAGC 1 cut(s) 1471
BccI CCATC 2 cut(s) 1875, 1897
BceAI ACGGC 2 cut(s) 279, 1645
BcgI CGANNNNNNTGC 2 cut(s) 1215, 1249
BcnI CCSGG 1 cut(s) 381
BcoDI GTCTC 8 cut(s) 9, 401, 555, 1398, 1579, 1879, 1891, 2013
BfaI CTAG 5 cut(s) 185, 1541, 1545, 1679, 1742
BfoI RGCGCY 1 cut(s) 1385
BfrI CTTAAG 1 cut(s) 1025
BisI GCNGC 2 cut(s) 1253, 1460
BlsI GCNGC 2 cut(s) 1254, 1461
Bme1390I CCNGG 1 cut(s) 381
BmgBI CACGTC 1 cut(s) 1901
BmiI GGNNCC 2 cut(s) 462, 1944
BmrFI CCNGG 1 cut(s) 381
BmsI GCATC 4 cut(s) 913, 1433, 1642, 2034
BpuEI CTTGAG 1 cut(s) 652
BpuMI CCSGG 1 cut(s) 381
Bsa29I ATCGAT 5 cut(s) 427, 578, 594, 696, 1235
BsaBI GATNNNNATC 2 cut(s) 147, 823
BsaHI GRCGYC 1 cut(s) 266
BsaI GGTCTC 3 cut(s) 401, 1398, 1879
BsaJI CCNNGG 2 cut(s) 136, 261
BsaWI WCCGGW 5 cut(s) 206, 247, 811, 943, 1939
Bse118I RCCGGY 1 cut(s) 326
Bse1I ACTGG 3 cut(s) 647, 1048, 1394
Bse8I GATNNNNATC 2 cut(s) 147, 823
BseAI TCCGGA 1 cut(s) 247
BseCI ATCGAT 5 cut(s) 427, 578, 594, 696, 1235
BseDI CCNNGG 2 cut(s) 136, 261
BseGI GGATG 3 cut(s) 80, 1801, 1867
BseJI GATNNNNATC 2 cut(s) 147, 823
BseMII CTCAG 5 cut(s) 425, 566, 753, 1023, 1632
BseNI ACTGG 3 cut(s) 647, 1048, 1394
BseRI GAGGAG 1 cut(s) 1134
BseSI GKGCMC 1 cut(s) 1867
BseXI GCAGC 1 cut(s) 1471
BseYI CCCAGC 1 cut(s) 1525
BshFI GGCC 1 cut(s) 1702
BshVI ATCGAT 5 cut(s) 427, 578, 594, 696, 1235
BsiSI CCGG 7 cut(s) 207, 248, 327, 381, 812, 944, 1940
BslFI GGGAC 2 cut(s) 363, 1177
BsmAI GTCTC 8 cut(s) 9, 401, 555, 1398, 1579, 1879, 1891, 2013
BsmBI CGTCTC 2 cut(s) 555, 1891
BsmFI GGGAC 2 cut(s) 363, 1177
BsnI GGCC 1 cut(s) 1702
Bso31I GGTCTC 3 cut(s) 401, 1398, 1879
Bsp1286I GDGCHC 3 cut(s) 174, 1571, 1867
Bsp13I TCCGGA 1 cut(s) 247
BspACI CCGC 4 cut(s) 324, 898, 1252, 1587
BspANI GGCC 1 cut(s) 1702
BspCNI CTCAG 5 cut(s) 424, 565, 752, 1024, 1633
BspDI ATCGAT 5 cut(s) 427, 578, 594, 696, 1235
BspEI TCCGGA 1 cut(s) 247
BspLI GGNNCC 2 cut(s) 462, 1944
BspTI CTTAAG 1 cut(s) 1025
BspTNI GGTCTC 3 cut(s) 401, 1398, 1879
BsrFI RCCGGY 1 cut(s) 326
BsrI ACTGG 3 cut(s) 647, 1048, 1394
BssAI RCCGGY 1 cut(s) 326
BssECI CCNNGG 2 cut(s) 136, 261
BssNI GRCGYC 1 cut(s) 266
Bst4CI ACNGT 6 cut(s) 161, 214, 295, 719, 1735, 1769
Bst6I CTCTTC 2 cut(s) 1111, 1771
BstACI GRCGYC 1 cut(s) 266
BstAFI CTTAAG 1 cut(s) 1025
BstC8I GCNNGC 2 cut(s) 1246, 1250
BstDEI CTNAG 7 cut(s) 391, 411, 552, 739, 1032, 1641, 2043
BstDSI CCRYGG 1 cut(s) 261
BstEII GGTNACC 1 cut(s) 1601
BstF5I GGATG 3 cut(s) 80, 1801, 1867
BstH2I RGCGCY 1 cut(s) 1385
BstHHI GCGC 1 cut(s) 1384
BstMAI GTCTC 8 cut(s) 9, 401, 555, 1398, 1579, 1879, 1891, 2013
BstMWI GCNNNNNNNGC 1 cut(s) 673
BstPI GGTNACC 1 cut(s) 1601
BstSCI CCNGG 1 cut(s) 379
BstSLI GKGCMC 1 cut(s) 1867
BstV1I GCAGC 1 cut(s) 1471
BstX2I RGATCY 4 cut(s) 313, 460, 535, 643
BstYI RGATCY 4 cut(s) 313, 460, 535, 643
Bsu15I ATCGAT 5 cut(s) 427, 578, 594, 696, 1235
BsuRI GGCC 1 cut(s) 1702
BsuTUI ATCGAT 5 cut(s) 427, 578, 594, 696, 1235
BtgI CCRYGG 1 cut(s) 261
BtrI CACGTC 1 cut(s) 1901
BtsCI GGATG 3 cut(s) 80, 1801, 1867
BtsIMutI CAGTG 3 cut(s) 1041, 1740, 1774
Cac8I GCNNGC 2 cut(s) 1246, 1250
CaiI CAGNNNCTG 2 cut(s) 1376, 1640
CfoI GCGC 1 cut(s) 1384
Cfr10I RCCGGY 1 cut(s) 326
ClaI ATCGAT 5 cut(s) 427, 578, 594, 696, 1235
CseI GACGC 1 cut(s) 255
Csp6I GTAC 4 cut(s) 210, 979, 1593, 2032
CviAII CATG 6 cut(s) 39, 58, 91, 1324, 1448, 1559
CviQI GTAC 4 cut(s) 210, 979, 1593, 2032
DdeI CTNAG 7 cut(s) 391, 411, 552, 739, 1032, 1641, 2043
Eam1104I CTCTTC 2 cut(s) 1111, 1771
EarI CTCTTC 2 cut(s) 1111, 1771
EciI GGCGGA 1 cut(s) 313
Eco24I GRGCYC 2 cut(s) 174, 1571
Eco31I GGTCTC 3 cut(s) 401, 1398, 1879
Eco57I CTGAAG 2 cut(s) 516, 1892
Eco91I GGTNACC 1 cut(s) 1601
EcoO65I GGTNACC 1 cut(s) 1601
EcoRI GAATTC 1 cut(s) 443
EcoT38I GRGCYC 2 cut(s) 174, 1571
Esp3I CGTCTC 2 cut(s) 555, 1891
FaeI CATG 6 cut(s) 42, 61, 94, 1327, 1451, 1562
FaqI GGGAC 2 cut(s) 363, 1177
FatI CATG 6 cut(s) 38, 57, 90, 1323, 1447, 1558
Fnu4HI GCNGC 2 cut(s) 1253, 1460
FokI GGATG 3 cut(s) 67, 1808, 1854
FriOI GRGCYC 2 cut(s) 174, 1571
Fsp4HI GCNGC 2 cut(s) 1253, 1460
FspBI CTAG 5 cut(s) 185, 1541, 1545, 1679, 1742
GlaI GCGC 1 cut(s) 1383
GluI GCNGC 2 cut(s) 1253, 1460
GsaI CCCAGC 1 cut(s) 1529
HaeII RGCGCY 1 cut(s) 1385
HaeIII GGCC 1 cut(s) 1702
HapII CCGG 7 cut(s) 207, 248, 327, 381, 812, 944, 1940
HgaI GACGC 1 cut(s) 255
HhaI GCGC 1 cut(s) 1384
Hin1I GRCGYC 1 cut(s) 266
Hin1II CATG 6 cut(s) 42, 61, 94, 1327, 1451, 1562
Hin6I GCGC 1 cut(s) 1382
HinP1I GCGC 1 cut(s) 1382
HincII GTYRAC 1 cut(s) 691
HindII GTYRAC 1 cut(s) 691
HindIII AAGCTT 1 cut(s) 17
HpaI GTTAAC 1 cut(s) 691
HpaII CCGG 7 cut(s) 207, 248, 327, 381, 812, 944, 1940
HphI GGTGA 9 cut(s) 283, 341, 731, 826, 874, 1310, 1436, 1684, 1823
Hpy166II GTNNAC 4 cut(s) 691, 1138, 1471, 2032
Hpy8I GTNNAC 4 cut(s) 691, 1138, 1471, 2032
Hpy99I CGWCG 1 cut(s) 437
HpyAV CCTTC 8 cut(s) 121, 298, 746, 1012, 1199, 1692, 1766, 2009
HpyCH4III ACNGT 6 cut(s) 161, 214, 295, 719, 1735, 1769
HpyCH4IV ACGT 4 cut(s) 613, 624, 1900, 2034
HpyCH4V TGCA 2 cut(s) 1276, 1765
HpyF10VI GCNNNNNNNGC 1 cut(s) 673
HpyF3I CTNAG 7 cut(s) 391, 411, 552, 739, 1032, 1641, 2043
HpySE526I ACGT 4 cut(s) 613, 624, 1900, 2034
Hsp92I GRCGYC 1 cut(s) 266
Hsp92II CATG 6 cut(s) 42, 61, 94, 1327, 1451, 1562
HspAI GCGC 1 cut(s) 1382
Kpn2I TCCGGA 1 cut(s) 247
KspAI GTTAAC 1 cut(s) 691
LmnI GCTCC 5 cut(s) 209, 1021, 1121, 1373, 1555
Lsp1109I GCAGC 1 cut(s) 1471
LweI GCATC 4 cut(s) 913, 1433, 1642, 2034
MaeI CTAG 5 cut(s) 185, 1541, 1545, 1679, 1742
MaeII ACGT 4 cut(s) 613, 624, 1900, 2034
MaeIII GTNAC 6 cut(s) 122, 329, 386, 649, 1286, 1601
MflI RGATCY 4 cut(s) 313, 460, 535, 643
MhlI GDGCHC 3 cut(s) 174, 1571, 1867
MluCI AATT 7 cut(s) 94, 403, 443, 455, 781, 1195, 1301
MlyI GAGTC 4 cut(s) 330, 1116, 1542, 1732
MmeI TCCRAC 1 cut(s) 341
MroI TCCGGA 1 cut(s) 247
MroXI GAANNNNTTC 3 cut(s) 428, 626, 1305
MslI CAYNNNNRTG 1 cut(s) 1452
MspA1I CMGCKG 1 cut(s) 1589
MspCI CTTAAG 1 cut(s) 1025
MspI CCGG 7 cut(s) 207, 248, 327, 381, 812, 944, 1940
MspR9I CCNGG 1 cut(s) 381
MwoI GCNNNNNNNGC 1 cut(s) 673
NciI CCSGG 1 cut(s) 381
NlaIII CATG 6 cut(s) 42, 61, 94, 1327, 1451, 1562
NlaIV GGNNCC 2 cut(s) 462, 1944
NmuCI GTSAC 2 cut(s) 329, 649
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 438
PdmI GAANNNNTTC 3 cut(s) 428, 626, 1305
PkrI GCNGC 2 cut(s) 1254, 1461
PleI GAGTC 4 cut(s) 330, 1115, 1542, 1731
PpsI GAGTC 4 cut(s) 330, 1115, 1542, 1731
PspEI GGTNACC 1 cut(s) 1601
PspFI CCCAGC 1 cut(s) 1525
PspN4I GGNNCC 2 cut(s) 462, 1944
PstNI CAGNNNCTG 2 cut(s) 1376, 1640
PsuI RGATCY 4 cut(s) 313, 460, 535, 643
RsaI GTAC 4 cut(s) 211, 980, 1594, 2033
RsaNI GTAC 4 cut(s) 210, 979, 1593, 2032
RseI CAYNNNNRTG 1 cut(s) 1452
SatI GCNGC 2 cut(s) 1253, 1460
SchI GAGTC 4 cut(s) 330, 1116, 1542, 1732
ScrFI CCNGG 1 cut(s) 381
SduI GDGCHC 3 cut(s) 174, 1571, 1867
SfaNI GCATC 4 cut(s) 913, 1433, 1642, 2034
SgrAI CRCCGGYG 1 cut(s) 326
SmiMI CAYNNNNRTG 1 cut(s) 1452
SmlI CTYRAG 2 cut(s) 631, 1025
SmoI CTYRAG 2 cut(s) 631, 1025
Sse9I AATT 7 cut(s) 94, 403, 443, 455, 781, 1195, 1301
SsiI CCGC 4 cut(s) 324, 898, 1252, 1587
SspMI CTAG 5 cut(s) 185, 1541, 1545, 1679, 1742
StyD4I CCNGG 1 cut(s) 379
TaaI ACNGT 6 cut(s) 161, 214, 295, 719, 1735, 1769
TaiI ACGT 4 cut(s) 616, 627, 1903, 2037
TasI AATT 7 cut(s) 94, 403, 443, 455, 781, 1195, 1301
TauI GCSGC 1 cut(s) 1255
TscAI CASTG 3 cut(s) 1048, 1740, 1774
TseFI GTSAC 2 cut(s) 329, 649
TseI GCWGC 1 cut(s) 1459
Tsp45I GTSAC 2 cut(s) 329, 649
TspDTI ATGAA 9 cut(s) 107, 434, 758, 1085, 1312, 1836, 1847, 1967, 2025
TspGWI ACGGA 6 cut(s) 110, 224, 348, 696, 1568, 1680
TspRI CASTG 3 cut(s) 1048, 1740, 1774
Vha464I CTTAAG 1 cut(s) 1025
XapI RAATTY 2 cut(s) 94, 443
XmnI GAANNNNTTC 3 cut(s) 428, 626, 1305
XspI CTAG 5 cut(s) 185, 1541, 1545, 1679, 1742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.