MD11G1030600.v1.1

Lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0005408
Physical Location & Seq
Forward (+)
2671424 .. 2672474
1051 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1030600.v1.1.491

Sequence Viewer

Length: 714 bp
ATGTTATTTTTTTCAAAATCTCCAAGTCCTCTGCTTTGGCAACGAAAATCGAAAACCCATTTGTTTCCATCCATCAGAACTCGAAATCTCCATATTCATGTCTCCAAGAAAGCTTCTTGTTTTCTGGGTCGACGTCTTCTTCCTCATAACTCTCTTCCTCCGCCGTGGCTCGGCCGAGAACGTCAACTTCTACTTCCCATCTTTGAACTTCCGAAACCTCACCTTCCTCGGCGACTCCCACATCCGAAACGGTGTCTTGGGGCTCACCGCGAGCTCACCGTCCCCTCCTCCAGCGCCGGTGCCGTCATCTACAACAACCCAATTCGGTTCTTCGACCCGGAGTCCAACATCACTGCTTCTTTCTCCACAAAGTTCGCTTTCTCCATCGCCAACGTCAATCCGAGCTCCTACGGCGACGGCATATCGTTTTTCCTCTCGCAGGAGAACTGGACGCTGGGCAGCCCTGGTGGATATCTGGGTCTCGTCAATTCCTCGCAATTGACCAAGAACAAGTTCGTAGCCGTCGAATTTGACACGAAGCTCGATCTTCACTTCGACGATCCGAACGACAATCAGGTCGGATTGGACATTGAGAGCCTCAATTCGATCAAGACCGCTGATCCGATTTCACAGGGTGTCGATTTCAAGGGCGGAATTCGATCACTGCTTGGATTGATTACAAGAACGACCAATAAAAGCTTAAAGTGTATCTGA

Protein Analysis

238

Amino Acids

26.48

Weight (kDa)

9.92

Isoelectric Point (pI)

49.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 97 - 216 1.1e-31 Legume lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 575
AatII GACGTC 1 cut(s) 136
AccB1I GGYRCC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 130
AccII CGCG 1 cut(s) 270
AciI CCGC 4 cut(s) 161, 268, 615, 651
AclWI GGATC 2 cut(s) 554, 614
AcoI YGGCCR 1 cut(s) 172
AcsI RAATTY 2 cut(s) 527, 654
AcyI GRCGYC 1 cut(s) 133
AdeI CACNNNGTG 1 cut(s) 635
AfiI CCNNNNNNNGG 2 cut(s) 170, 439
AgsI TTSAA 3 cut(s) 15, 206, 646
AhdI GACNNNNNGTC 1 cut(s) 340
AjnI CCWGG 1 cut(s) 463
AjuI GAANNNNNNNTTGG 2 cut(s) 497, 529
AluBI AGCT 5 cut(s) 113, 274, 405, 541, 699
AluI AGCT 5 cut(s) 113, 274, 405, 541, 699
Alw21I GWGCWC 2 cut(s) 276, 407
Alw26I GTCTC 2 cut(s) 106, 485
AlwI GGATC 2 cut(s) 554, 614
AoxI GGCC 1 cut(s) 172
ApeKI GCWGC 1 cut(s) 459
ApoI RAATTY 2 cut(s) 527, 654
Asp700I GAANNNNTTC 1 cut(s) 512
AspLEI GCGC 1 cut(s) 296
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 3 cut(s) 212, 257, 268
BanI GGYRCC 1 cut(s) 299
BanII GRGCYC 3 cut(s) 265, 276, 407
BbsI GAAGAC 1 cut(s) 128
Bbv12I GWGCWC 2 cut(s) 276, 407
BbvI GCAGC 1 cut(s) 471
BccI CCATC 4 cut(s) 76, 80, 206, 392
BceAI ACGGC 5 cut(s) 148, 287, 427, 433, 506
BciT130I CCWGG 1 cut(s) 465
BcnI CCSGG 1 cut(s) 338
BcoDI GTCTC 2 cut(s) 106, 485
BfoI RGCGCY 1 cut(s) 297
BisI GCNGC 1 cut(s) 460
BlsI GCNGC 1 cut(s) 461
Bme1390I CCNGG 2 cut(s) 338, 465
BmeRI GACNNNNNGTC 1 cut(s) 340
BmiI GGNNCC 1 cut(s) 301
BmrFI CCNGG 2 cut(s) 338, 465
BpiI GAAGAC 1 cut(s) 128
BpmI CTGGAG 1 cut(s) 274
BpuMI CCSGG 1 cut(s) 338
BsaHI GRCGYC 1 cut(s) 133
BsaI GGTCTC 1 cut(s) 485
BsaJI CCNNGG 3 cut(s) 164, 227, 463
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 439
Bse118I RCCGGY 1 cut(s) 296
Bse1I ACTGG 1 cut(s) 452
BseBI CCWGG 1 cut(s) 465
BseDI CCNNGG 3 cut(s) 164, 227, 463
BseGI GGATG 2 cut(s) 68, 241
BseLI CCNNNNNNNGG 2 cut(s) 170, 439
BseNI ACTGG 1 cut(s) 452
BseRI GAGGAG 1 cut(s) 277
BseX3I CGGCCG 1 cut(s) 172
BseXI GCAGC 1 cut(s) 471
BseYI CCCAGC 1 cut(s) 454
Bsh1236I CGCG 1 cut(s) 270
Bsh1285I CGRYCG 1 cut(s) 175
BshFI GGCC 1 cut(s) 174
BshNI GGYRCC 1 cut(s) 299
BsiEI CGRYCG 1 cut(s) 175
BsiHKAI GWGCWC 2 cut(s) 276, 407
BsiSI CCGG 2 cut(s) 297, 338
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 2 cut(s) 170, 439
BsmAI GTCTC 2 cut(s) 106, 485
BsmFI GGGAC 1 cut(s) 266
BsnI GGCC 1 cut(s) 174
Bso31I GGTCTC 1 cut(s) 485
Bsp1286I GDGCHC 3 cut(s) 265, 276, 407
Bsp143I GATC 5 cut(s) 544, 559, 606, 619, 659
BspACI CCGC 4 cut(s) 161, 268, 615, 651
BspANI GGCC 1 cut(s) 174
BspFNI CGCG 1 cut(s) 270
BspLI GGNNCC 1 cut(s) 301
BspPI GGATC 2 cut(s) 554, 614
BspT107I GGYRCC 1 cut(s) 299
BspTNI GGTCTC 1 cut(s) 485
BsrFI RCCGGY 1 cut(s) 296
BsrI ACTGG 1 cut(s) 452
BssAI RCCGGY 1 cut(s) 296
BssECI CCNNGG 3 cut(s) 164, 227, 463
BssMI GATC 5 cut(s) 544, 559, 606, 619, 659
BssNI GRCGYC 1 cut(s) 133
Bst2UI CCWGG 1 cut(s) 465
Bst4CI ACNGT 2 cut(s) 252, 280
Bst6I CTCTTC 1 cut(s) 159
BstACI GRCGYC 1 cut(s) 133
BstC8I GCNNGC 1 cut(s) 272
BstDSI CCRYGG 1 cut(s) 164
BstENI CCTNNNNNAGG 1 cut(s) 437
BstF5I GGATG 2 cut(s) 68, 241
BstFNI CGCG 1 cut(s) 270
BstH2I RGCGCY 1 cut(s) 297
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 5 cut(s) 547, 562, 609, 622, 662
BstMAI GTCTC 2 cut(s) 106, 485
BstMBI GATC 5 cut(s) 544, 559, 606, 619, 659
BstMCI CGRYCG 1 cut(s) 175
BstMWI GCNNNNNNNGC 1 cut(s) 411
BstNI CCWGG 1 cut(s) 465
BstSCI CCNGG 2 cut(s) 336, 463
BstUI CGCG 1 cut(s) 270
BstV1I GCAGC 1 cut(s) 471
BstV2I GAAGAC 1 cut(s) 128
BstZI CGGCCG 1 cut(s) 172
BsuRI GGCC 1 cut(s) 174
BtgI CCRYGG 1 cut(s) 164
BtgZI GCGATG 1 cut(s) 370
BtsCI GGATG 2 cut(s) 68, 241
BtsI GCAGTG 2 cut(s) 351, 662
BtsIMutI CAGTG 2 cut(s) 351, 662
Cac8I GCNNGC 1 cut(s) 272
CfoI GCGC 1 cut(s) 296
Cfr10I RCCGGY 1 cut(s) 296
CseI GACGC 1 cut(s) 460
CviAII CATG 1 cut(s) 98
DpnI GATC 5 cut(s) 546, 561, 608, 621, 661
DpnII GATC 5 cut(s) 544, 559, 606, 619, 659
DraIII CACNNNGTG 1 cut(s) 635
DrdI GACNNNNNNGTC 1 cut(s) 575
DriI GACNNNNNGTC 1 cut(s) 340
DseDI GACNNNNNNGTC 1 cut(s) 575
EaeI YGGCCR 1 cut(s) 172
EagI CGGCCG 1 cut(s) 172
Eam1104I CTCTTC 1 cut(s) 159
Eam1105I GACNNNNNGTC 1 cut(s) 340
EarI CTCTTC 1 cut(s) 159
EciI GGCGGA 2 cut(s) 150, 666
Ecl136II GAGCTC 2 cut(s) 274, 405
EclXI CGGCCG 1 cut(s) 172
Eco24I GRGCYC 3 cut(s) 265, 276, 407
Eco31I GGTCTC 1 cut(s) 485
Eco32I GATATC 1 cut(s) 473
Eco52I CGGCCG 1 cut(s) 172
Eco53kI GAGCTC 2 cut(s) 274, 405
EcoICRI GAGCTC 2 cut(s) 274, 405
EcoNI CCTNNNNNAGG 1 cut(s) 437
EcoRI GAATTC 1 cut(s) 654
EcoRII CCWGG 1 cut(s) 463
EcoRV GATATC 1 cut(s) 473
EcoT38I GRGCYC 3 cut(s) 265, 276, 407
FaeI CATG 1 cut(s) 101
FaiI YATR 4 cut(s) 93, 99, 147, 422
FaqI GGGAC 1 cut(s) 266
FatI CATG 1 cut(s) 97
FblI GTMKAC 1 cut(s) 130
Fnu4HI GCNGC 1 cut(s) 460
FokI GGATG 2 cut(s) 55, 228
FriOI GRGCYC 3 cut(s) 265, 276, 407
Fsp4HI GCNGC 1 cut(s) 460
GlaI GCGC 1 cut(s) 295
GluI GCNGC 1 cut(s) 460
GsaI CCCAGC 1 cut(s) 458
GsuI CTGGAG 1 cut(s) 274
HaeII RGCGCY 1 cut(s) 297
HaeIII GGCC 1 cut(s) 174
HapII CCGG 2 cut(s) 297, 338
HgaI GACGC 1 cut(s) 460
HhaI GCGC 1 cut(s) 296
Hin1I GRCGYC 1 cut(s) 133
Hin1II CATG 1 cut(s) 101
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HincII GTYRAC 2 cut(s) 131, 185
HindII GTYRAC 2 cut(s) 131, 185
HindIII AAGCTT 2 cut(s) 111, 697
HinfI GANTC 2 cut(s) 234, 341
HpaII CCGG 2 cut(s) 297, 338
HphI GGTGA 3 cut(s) 212, 257, 268
Hpy166II GTNNAC 2 cut(s) 131, 185
Hpy188I TCNGA 8 cut(s) 77, 213, 246, 402, 564, 581, 624, 713
Hpy188III TCNNGA 1 cut(s) 610
Hpy8I GTNNAC 2 cut(s) 131, 185
Hpy99I CGWCG 4 cut(s) 135, 419, 527, 560
HpyAV CCTTC 1 cut(s) 233
HpyCH4III ACNGT 2 cut(s) 252, 280
HpyCH4IV ACGT 3 cut(s) 133, 181, 393
HpyF10VI GCNNNNNNNGC 1 cut(s) 411
HpySE526I ACGT 3 cut(s) 133, 181, 393
Hsp92I GRCGYC 1 cut(s) 133
Hsp92II CATG 1 cut(s) 101
HspAI GCGC 1 cut(s) 294
Kzo9I GATC 5 cut(s) 544, 559, 606, 619, 659
LmnI GCTCC 1 cut(s) 410
Lsp1109I GCAGC 1 cut(s) 471
MaeII ACGT 3 cut(s) 133, 181, 393
MalI GATC 5 cut(s) 546, 561, 608, 621, 661
MboI GATC 5 cut(s) 544, 559, 606, 619, 659
MboII GAAGA 5 cut(s) 128, 131, 146, 322, 539
MfeI CAATTG 1 cut(s) 497
MhlI GDGCHC 3 cut(s) 265, 276, 407
MluCI AATT 6 cut(s) 321, 487, 497, 527, 601, 654
MlyI GAGTC 2 cut(s) 228, 350
MmeI TCCRAC 2 cut(s) 369, 559
MroXI GAANNNNTTC 1 cut(s) 512
MseI TTAA 1 cut(s) 701
MslI CAYNNNNRTG 1 cut(s) 96
MspA1I CMGCKG 1 cut(s) 617
MspI CCGG 2 cut(s) 297, 338
MspR9I CCNGG 2 cut(s) 338, 465
MunI CAATTG 1 cut(s) 497
MvaI CCWGG 1 cut(s) 465
MvnI CGCG 1 cut(s) 270
MwoI GCNNNNNNNGC 1 cut(s) 411
NciI CCSGG 1 cut(s) 338
NdeII GATC 5 cut(s) 544, 559, 606, 619, 659
NlaIII CATG 1 cut(s) 101
NlaIV GGNNCC 1 cut(s) 301
NmeAIII GCCGAG 3 cut(s) 150, 200, 208
PcsI WCGNNNNNNNCGW 2 cut(s) 522, 564
PdmI GAANNNNTTC 1 cut(s) 512
PkrI GCNGC 1 cut(s) 461
PleI GAGTC 2 cut(s) 228, 349
PpsI GAGTC 2 cut(s) 228, 349
Psp124BI GAGCTC 2 cut(s) 276, 407
Psp6I CCWGG 1 cut(s) 463
PspFI CCCAGC 1 cut(s) 454
PspGI CCWGG 1 cut(s) 463
PspN4I GGNNCC 1 cut(s) 301
RseI CAYNNNNRTG 1 cut(s) 96
SacI GAGCTC 2 cut(s) 276, 407
SalI GTCGAC 1 cut(s) 129
SaqAI TTAA 1 cut(s) 701
SatI GCNGC 1 cut(s) 460
Sau3AI GATC 5 cut(s) 544, 559, 606, 619, 659
SchI GAGTC 2 cut(s) 228, 350
ScrFI CCNGG 2 cut(s) 338, 465
SduI GDGCHC 3 cut(s) 265, 276, 407
SgrAI CRCCGGYG 1 cut(s) 296
SmiMI CAYNNNNRTG 1 cut(s) 96
Sse9I AATT 6 cut(s) 321, 487, 497, 527, 601, 654
SsiI CCGC 4 cut(s) 161, 268, 615, 651
SstI GAGCTC 2 cut(s) 276, 407
StyD4I CCNGG 2 cut(s) 336, 463
TaaI ACNGT 2 cut(s) 252, 280
TaiI ACGT 3 cut(s) 136, 184, 396
TasI AATT 6 cut(s) 321, 487, 497, 527, 601, 654
Tru1I TTAA 1 cut(s) 701
Tru9I TTAA 1 cut(s) 701
TscAI CASTG 2 cut(s) 358, 669
TseI GCWGC 1 cut(s) 459
TspDTI ATGAA 1 cut(s) 86
TspRI CASTG 2 cut(s) 358, 669
XagI CCTNNNNNAGG 1 cut(s) 437
XapI RAATTY 2 cut(s) 527, 654
XmiI GTMKAC 1 cut(s) 130
XmnI GAANNNNTTC 1 cut(s) 512
ZraI GACGTC 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.