Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0005408

Physical Location & Seq

Genomic Coordinates Forward (+)

81624463 .. 81626760

Gene Structure 2298 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G047130.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2016 bp

ATGAACCCAAGAAAGCTTCTTGCTTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCCGCCGCTTCTCCTCCGCCGCCGCCGAGAACCTCAGCTTCGACTTCCCCTCCTTCAACTTCCGCAACCTCACCTTCCTCGGCTCCTCCCACATCCGCAACGGCGTCGTCGGCCTCACCCGCGACCTCCCCGTCCCCACCTCCTCCGCCGGCTCCATCCTCTACAACTCCCCCGTCCGCTTCTTCGACCCCGACTCCAACACCACCGCCTCCTTCTCCACCCGCTTCACCTTCTCCTTCTCCAACCTCAACCCCTCCTCCTCCGGCGACGGCATGTCGTTTTTCCTCTCCCCCAACAACCAGACCCTCGGCAGCTCCGGCGCCTACCTGGGTCTCCTCAACTCCTCCCTCCCCACCCACAACCACTTCATCGCCGTCGAATTCGACACCCGCCAAGATACCCACTTTTCCGACCCGAACGACAACCACGTCGGTCTCGACATCGACACCTTGATCTCCATCAAGACCGCCGACCCCATTGACCAAGATGTTGACTTGAAGAGCGGAACGGCGATCACGGCCTGGATTGACTACAAGAACGACCAAGAAACGCTTAAGGTGTACCTGAGCTACTCCGCGTTCAAACCCGAGACCCCTGTTTTGAACGTGGGGATTGATCTTTCTGGGTATCTGAAGGAGGTTATGTATGTGGGGTTCTCTGCTTCAACTGAAGGAAGCACCGAGCTTCATGAGATTGAGAATTGGAGCTTCAGGACATCTGGGTTTGTGCCGAAAAGGCCCACATTGCGTCCTCACAATGTGTCTGATACTTATGTGATTGTCAGTCCAAGGCTGCCGGCGCCGGAGAAGGGACACAAGAGGCGGCTGGGGCTGGGGCTGGGGATTGCCGGGCCGGCGTTCTTTTGTGTTGCGCTGGTGGTTTTCGGGTATGTGAGTGTGAGGAAGTGGAGGGAGGTGAGGAGGGTGAAGAGCTTTAAAGCAGAGGTGGTGGCAGGGCCGAGGCAGTTTAGCTATAGAGAGCTGAGGGCGGCTACAGGAGGGTTTCATGGGAGTAGGATTATTGGGCATGGCGCATTTGGGACTGTGTACAAGGCGTTTTTCGTGGCGTCGGGGACTATTGCGGCGGTGAAGAGGTCCAGGCATTCTCATGAAGGTAAGACTGAGTTTCTTGCTGAGTTGTCTATTATAGCTTGCTTGAGGCACAAGAATTTGGTGCATTTGCAAGGGTGGTGTGTTGAGAAAGGTGAGTTGCTGCTTGTGTATGATTTCATGCCGAATGGTAGTCTTGAGAGAGTGCTTTACCAAGAAAGTGAGGAGGGTAACTTGTTGGATTGGCCTCATAGGCAAAATGTTGCGATTGGTTTGGCGTCTGTTTTGACGTATATGCATCAAGAATGTGAGCAGCAAGTGATTCATAGGGATATTAAGACTGGTAATGTGTTGCTGGATGGGAATTTCAATGCAAAGCTTGGTGATTTTGGATTGGCAAAGCTCATGGATCATGATAAGAGCCCTGTTTCAACGCTGACAGCTGGAACAATGGGGTACCTTGCGCCCGAGTATCTCCAGTATGGGAAAGCAACTGAGAAGACTGATGCTTTCAGCTATGGTGTGGTTATACTTGAATTGGCTTGTGGGAGGAGACCAATTGAGAGAATTCCGGATTGTCACAAAATGGTGAATTTGGTTGATTGGGTTTGGGGACTGCATTCTGAAGGAAGGATTATTGATGCAGCTGATCAGAGGTTGGCTGGGGAGTTTGATGTGGAAGAAATGCGGAAGCTGTTGCTTGTCGGTTTGAGTTGTGCTAACCCAGATAGCTCCAAGAGGCCTACAATGAGGAGAGTTCTACAGATCTTGAGTAATGAGGCAGAACCTCCAGCTGTGCCGAGGGTTAAGCCAAGTCTCAGTTTCTCTTGTGGCGTGTCTTTGTCTGTTGATGACATTGTTTCAGATTGTGATGGCAGCCTCACCAGCTCAATGTGCGAGATTGTTGTCAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002229 GO:0002239 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0016020 GO:0043207 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169

Pfam Domains

Protein Families

Lectin_legB Pkinase Pkinase_Tyr

Protein Analysis

671

Amino Acids

74.42

Weight (kDa)

6.48

Isoelectric Point (pI)

42.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lectin_legB	PF00139	29 - 262	9.9e-66	Legume lectin domain
Lectin_L-type_dom	PF18483	49 - 250	7.9e-13	Legume lectin beta-barrel domain
Pkinase	PF00069	353 - 622	1.4e-47	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	356 - 622	1e-41	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005408)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G55830
fragaria_vesca	FvH4_2g31170 FvH4_3g42990 FvH4_4g09342 FvH4_4g11641
malus_domestica	MD03G1026600.v1.1 MD11G1030600.v1.1
prunus_persica	Prupe.6G024000_v2.0.a1
pyrus_communis	pycom03g02180 pycom11g02420 pycom11g02430 pycom11g02480
rosa_chinensis	RchiOBHm_Chr5g0077031
rosa_laevigata	RLG00000036643
rosa_multiflora	Rmu_sc0003323.1_g000008 Rmu_sc0018789.1_g000001 Rmu_ssc0000470.1_g000027
rosa_roxburghii	Rroxscaffold_1G00005100
rosa_rugosa	Rorug05G0450500
rosa_samantha	Rh5AG505100 Rh5BG416900 Rh5BG526800 Rh5CG441400 Rh5CG550600 Rh5DG539000 Rh6AG352200 Rh6CG162900 Rh6DG154500
rosa_wichuraiana	Rw5G047130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	182, 479
Acc65I	GGTACC	1 cut(s)	1557
AccB1I	GGYRCC	3 cut(s)	371, 850, 1557
AccBSI	CCGCTC	1 cut(s)	555
AccII	CGCG	2 cut(s)	174, 629
AccIII	TCCGGA	1 cut(s)	1672
AclWI	GGATC	1 cut(s)	1518
AcsI	RAATTY	5 cut(s)	431, 1219, 1465, 1668, 1693
AcuI	CTGAAG	4 cut(s)	704, 741, 745, 1746
AcyI	GRCGYC	5 cut(s)	156, 372, 851, 1118, 1379
AdeI	CACNNNGTG	1 cut(s)	811
AfaI	GTAC	3 cut(s)	614, 1100, 1559
AfiI	CCNNNNNNNGG	1 cut(s)	860
AflII	CTTAAG	1 cut(s)	605
AgsI	TTSAA	8 cut(s)	109, 550, 634, 655, 717, 1471, 1533, 1637
AhdI	GACNNNNNGTC	1 cut(s)	325
AjiI	CACGTC	1 cut(s)	481
AjnI	CCWGG	3 cut(s)	378, 572, 1148
Alw26I	GTCTC	5 cut(s)	389, 491, 635, 1648, 1922
AlwI	GGATC	1 cut(s)	1518
Ama87I	CYCGRG	2 cut(s)	638, 1568
Aor13HI	TCCGGA	1 cut(s)	1672
AoxI	GGCC	7 cut(s)	163, 570, 788, 902, 1007, 1346, 1841
ApeKI	GCWGC	6 cut(s)	363, 844, 1264, 1414, 1745, 1977
ApoI	RAATTY	5 cut(s)	431, 1219, 1465, 1668, 1693
ArsI	GACNNNNNNTTYG	2 cut(s)	634, 666
Asp718I	GGTACC	1 cut(s)	1557
AspLEI	GCGC	5 cut(s)	374, 853, 925, 1085, 1567
AspS9I	GGNCC	4 cut(s)	789, 902, 1007, 1146
AsuC2I	CCSGG	1 cut(s)	901
AvaI	CYCGRG	2 cut(s)	638, 1568
AvaII	GGWCC	1 cut(s)	1146
BaeI	ACNNNNGTAYC	2 cut(s)	810, 843
BanI	GGYRCC	3 cut(s)	371, 850, 1557
BanII	GRGCYC	1 cut(s)	1526
BbsI	GAAGAC	1 cut(s)	1607
BbvCI	CCTCAGC	2 cut(s)	86, 1034
BbvI	GCAGC	6 cut(s)	375, 831, 1251, 1426, 1757, 1989
BccI	CCATC	4 cut(s)	215, 518, 1454, 1967
BceAI	ACGGC	5 cut(s)	169, 337, 410, 576, 585
BcgI	CGANNNNNNTGC	2 cut(s)	139, 173
BciT130I	CCWGG	3 cut(s)	380, 574, 1150
BclI	TGATCA	1 cut(s)	1750
BcnI	CCSGG	1 cut(s)	901
BcoDI	GTCTC	5 cut(s)	389, 491, 635, 1648, 1922
BfmI	CTRYAG	3 cut(s)	1024, 1044, 1862
BfoI	RGCGCY	2 cut(s)	375, 854
BfrI	CTTAAG	1 cut(s)	605
BglI	GCCNNNNNGGC	3 cut(s)	787, 905, 1354
BglII	AGATCT	1 cut(s)	1866
Bme1390I	CCNGG	4 cut(s)	380, 574, 901, 1150
Bme18I	GGWCC	1 cut(s)	1146
BmeRI	GACNNNNNGTC	1 cut(s)	325
BmeT110I	CYCGRG	2 cut(s)	638, 1568
BmgBI	CACGTC	1 cut(s)	481
BmgT120I	GGNCC	4 cut(s)	789, 902, 1007, 1146
BmiI	GGNNCC	5 cut(s)	136, 205, 373, 852, 1559
BmrFI	CCNGG	4 cut(s)	380, 574, 901, 1150
BmsI	GCATC	3 cut(s)	1408, 1597, 1732
BpiI	GAAGAC	1 cut(s)	1607
BpmI	CTGGAG	2 cut(s)	1562, 1875
Bpu10I	CCTNAGC	3 cut(s)	86, 617, 1034
BpuEI	CTTGAG	3 cut(s)	1228, 1319, 1891
BpuMI	CCSGG	1 cut(s)	901
BsaBI	GATNNNNATC	1 cut(s)	509
BsaHI	GRCGYC	5 cut(s)	156, 372, 851, 1118, 1379
BsaI	GGTCTC	4 cut(s)	389, 491, 635, 1648
BsaJI	CCNNGG	6 cut(s)	130, 358, 379, 839, 1010, 1901
BsaWI	WCCGGW	1 cut(s)	1672
BsaXI	ACNNNNNCTCC	8 cut(s)	86, 116, 230, 260, 293, 323, 680, 710
Bsc4I	CCNNNNNNNGG	1 cut(s)	860
Bse118I	RCCGGY	3 cut(s)	200, 847, 904
Bse1I	ACTGG	2 cut(s)	1447, 1579
Bse3DI	GCAATG	1 cut(s)	794
Bse8I	GATNNNNATC	1 cut(s)	509
BseAI	TCCGGA	1 cut(s)	1672
BseBI	CCWGG	3 cut(s)	380, 574, 1150
BseDI	CCNNGG	6 cut(s)	130, 358, 379, 839, 1010, 1901
BseGI	GGATG	3 cut(s)	144, 207, 1465
BseJI	GATNNNNATC	1 cut(s)	509
BseLI	CCNNNNNNNGG	1 cut(s)	860
BseMI	GCAATG	1 cut(s)	794
BseMII	CTCAG	7 cut(s)	100, 608, 1025, 1164, 1176, 1587, 1933
BseNI	ACTGG	2 cut(s)	1447, 1579
BseXI	GCAGC	6 cut(s)	375, 831, 1251, 1426, 1757, 1989
BseYI	CCCAGC	4 cut(s)	877, 883, 889, 1763
Bsh1236I	CGCG	2 cut(s)	174, 629
BshFI	GGCC	7 cut(s)	165, 572, 790, 904, 1009, 1348, 1843
BshNI	GGYRCC	3 cut(s)	371, 850, 1557
BsiHKCI	CYCGRG	2 cut(s)	638, 1568
BsiSI	CCGG	8 cut(s)	201, 315, 369, 848, 854, 900, 905, 1673
BslFI	GGGAC	5 cut(s)	170, 876, 1105, 1138, 1728
BslI	CCNNNNNNNGG	1 cut(s)	860
BsmAI	GTCTC	5 cut(s)	389, 491, 635, 1648, 1922
BsmFI	GGGAC	5 cut(s)	170, 876, 1105, 1138, 1728
BsmI	GAATGC	2 cut(s)	1153, 1720
BsnI	GGCC	7 cut(s)	165, 572, 790, 904, 1009, 1348, 1843
Bso31I	GGTCTC	4 cut(s)	389, 491, 635, 1648
BsoBI	CYCGRG	2 cut(s)	638, 1568
Bsp1286I	GDGCHC	1 cut(s)	1526
Bsp13I	TCCGGA	1 cut(s)	1672
Bsp1407I	TGTACA	1 cut(s)	1098
Bsp143I	GATC	6 cut(s)	504, 564, 667, 1510, 1750, 1866
BspANI	GGCC	7 cut(s)	165, 572, 790, 904, 1009, 1348, 1843
BspCNI	CTCAG	7 cut(s)	99, 609, 1026, 1165, 1177, 1588, 1932
BspEI	TCCGGA	1 cut(s)	1672
BspFNI	CGCG	2 cut(s)	174, 629
BspHI	TCATGA	3 cut(s)	739, 1159, 1513
BspLI	GGNNCC	5 cut(s)	136, 205, 373, 852, 1559
BspPI	GGATC	1 cut(s)	1518
BspQI	GCTCTTC	2 cut(s)	545, 974
BspT107I	GGYRCC	3 cut(s)	371, 850, 1557
BspTI	CTTAAG	1 cut(s)	605
BspTNI	GGTCTC	4 cut(s)	389, 491, 635, 1648
BsrBI	CCGCTC	1 cut(s)	555
BsrDI	GCAATG	1 cut(s)	794
BsrFI	RCCGGY	3 cut(s)	200, 847, 904
BsrGI	TGTACA	1 cut(s)	1098
BsrI	ACTGG	2 cut(s)	1447, 1579
BssAI	RCCGGY	3 cut(s)	200, 847, 904
BssECI	CCNNGG	6 cut(s)	130, 358, 379, 839, 1010, 1901
BssMI	GATC	6 cut(s)	504, 564, 667, 1510, 1750, 1866
BssNI	GRCGYC	5 cut(s)	156, 372, 851, 1118, 1379
BssT1I	CCWWGG	1 cut(s)	839
Bst2UI	CCWGG	3 cut(s)	380, 574, 1150
Bst4CI	ACNGT	1 cut(s)	1096
Bst6I	CTCTTC	3 cut(s)	545, 974, 1136
BstACI	GRCGYC	5 cut(s)	156, 372, 851, 1118, 1379
BstAFI	CTTAAG	1 cut(s)	605
BstAUI	TGTACA	1 cut(s)	1098
BstC8I	GCNNGC	4 cut(s)	202, 849, 906, 1204
BstDEI	CTNAG	7 cut(s)	86, 617, 1034, 1173, 1185, 1596, 1919
BstF5I	GGATG	3 cut(s)	144, 207, 1465
BstFNI	CGCG	2 cut(s)	174, 629
BstH2I	RGCGCY	2 cut(s)	375, 854
BstHHI	GCGC	5 cut(s)	374, 853, 925, 1085, 1567
BstKTI	GATC	6 cut(s)	507, 567, 670, 1513, 1753, 1869
BstMAI	GTCTC	5 cut(s)	389, 491, 635, 1648, 1922
BstMBI	GATC	6 cut(s)	504, 564, 667, 1510, 1750, 1866
BstNI	CCWGG	3 cut(s)	380, 574, 1150
BstNSI	RCATGY	1 cut(s)	328
BstSCI	CCNGG	4 cut(s)	378, 572, 899, 1148
BstSFI	CTRYAG	3 cut(s)	1024, 1044, 1862
BstUI	CGCG	2 cut(s)	174, 629
BstV1I	GCAGC	6 cut(s)	375, 831, 1251, 1426, 1757, 1989
BstV2I	GAAGAC	1 cut(s)	1607
BstX2I	RGATCY	1 cut(s)	1866
BstYI	RGATCY	1 cut(s)	1866
BsuRI	GGCC	7 cut(s)	165, 572, 790, 904, 1009, 1348, 1843
BtgZI	GCGATG	1 cut(s)	406
BtrI	CACGTC	1 cut(s)	481
BtsCI	GGATG	3 cut(s)	144, 207, 1465
Cac8I	GCNNGC	4 cut(s)	202, 849, 906, 1204
CciI	TCATGA	3 cut(s)	739, 1159, 1513
CfoI	GCGC	5 cut(s)	374, 853, 925, 1085, 1567
Cfr10I	RCCGGY	3 cut(s)	200, 847, 904
Cfr13I	GGNCC	4 cut(s)	789, 902, 1007, 1146
CseI	GACGC	4 cut(s)	145, 788, 1107, 1368
Csp6I	GTAC	3 cut(s)	613, 1099, 1558
CviAII	CATG	8 cut(s)	325, 740, 1058, 1079, 1160, 1282, 1507, 1514
CviQI	GTAC	3 cut(s)	613, 1099, 1558
DdeI	CTNAG	7 cut(s)	86, 617, 1034, 1173, 1185, 1596, 1919
DinI	GGCGCC	2 cut(s)	373, 852
DpnI	GATC	6 cut(s)	506, 566, 669, 1512, 1752, 1868
DpnII	GATC	6 cut(s)	504, 564, 667, 1510, 1750, 1866
DraI	TTTAAA	1 cut(s)	988
DraIII	CACNNNGTG	1 cut(s)	811
DrdI	GACNNNNNNGTC	2 cut(s)	182, 479
DriI	GACNNNNNGTC	1 cut(s)	325
DseDI	GACNNNNNNGTC	2 cut(s)	182, 479
Eam1104I	CTCTTC	3 cut(s)	545, 974, 1136
Eam1105I	GACNNNNNGTC	1 cut(s)	325
EarI	CTCTTC	3 cut(s)	545, 974, 1136
EciI	GGCGGA	3 cut(s)	44, 58, 187
Eco130I	CCWWGG	1 cut(s)	839
Eco147I	AGGCCT	1 cut(s)	1843
Eco24I	GRGCYC	1 cut(s)	1526
Eco31I	GGTCTC	4 cut(s)	389, 491, 635, 1648
Eco47I	GGWCC	1 cut(s)	1146
Eco57I	CTGAAG	4 cut(s)	704, 741, 745, 1746
Eco88I	CYCGRG	2 cut(s)	638, 1568
EcoRI	GAATTC	2 cut(s)	431, 1668
EcoRII	CCWGG	3 cut(s)	378, 572, 1148
EcoT14I	CCWWGG	1 cut(s)	839
EcoT22I	ATGCAT	1 cut(s)	1401
EcoT38I	GRGCYC	1 cut(s)	1526
EgeI	GGCGCC	2 cut(s)	373, 852
EheI	GGCGCC	2 cut(s)	373, 852
ErhI	CCWWGG	1 cut(s)	839
FaeI	CATG	8 cut(s)	328, 743, 1061, 1082, 1163, 1285, 1510, 1517
FalI	AAGNNNNNCTT	2 cut(s)	588, 620
FaqI	GGGAC	5 cut(s)	170, 876, 1105, 1138, 1728
FatI	CATG	8 cut(s)	324, 739, 1057, 1078, 1159, 1281, 1506, 1513
FauI	CCCGC	3 cut(s)	179, 281, 449
FbaI	TGATCA	1 cut(s)	1750
FokI	GGATG	3 cut(s)	131, 194, 1472
FriOI	GRGCYC	1 cut(s)	1526
GlaI	GCGC	5 cut(s)	373, 852, 924, 1084, 1566
GsaI	CCCAGC	4 cut(s)	881, 887, 893, 1767
GsuI	CTGGAG	2 cut(s)	1562, 1875
HaeII	RGCGCY	2 cut(s)	375, 854
HaeIII	GGCC	7 cut(s)	165, 572, 790, 904, 1009, 1348, 1843
HapII	CCGG	8 cut(s)	201, 315, 369, 848, 854, 900, 905, 1673
HgaI	GACGC	4 cut(s)	145, 788, 1107, 1368
HhaI	GCGC	5 cut(s)	374, 853, 925, 1085, 1567
Hin1I	GRCGYC	5 cut(s)	156, 372, 851, 1118, 1379
Hin1II	CATG	8 cut(s)	328, 743, 1061, 1082, 1163, 1285, 1510, 1517
Hin6I	GCGC	5 cut(s)	372, 851, 923, 1083, 1565
HinP1I	GCGC	5 cut(s)	372, 851, 923, 1083, 1565
HincII	GTYRAC	2 cut(s)	544, 2011
HindII	GTYRAC	2 cut(s)	544, 2011
HindIII	AAGCTT	2 cut(s)	14, 1478
HinfI	GANTC	2 cut(s)	245, 1423
HpaII	CCGG	8 cut(s)	201, 315, 369, 848, 854, 900, 905, 1673
Hpy166II	GTNNAC	4 cut(s)	544, 613, 1099, 2011
Hpy188I	TCNGA	6 cut(s)	463, 684, 817, 1726, 1755, 1966
Hpy8I	GTNNAC	4 cut(s)	544, 613, 1099, 2011
Hpy99I	CGWCG	6 cut(s)	161, 164, 323, 431, 485, 1123
HpyCH4III	ACNGT	1 cut(s)	1096
HpyCH4IV	ACGT	3 cut(s)	480, 657, 1391
HpyCH4V	TGCA	6 cut(s)	1228, 1234, 1399, 1475, 1720, 1745
HpyF3I	CTNAG	7 cut(s)	86, 617, 1034, 1173, 1185, 1596, 1919
HpySE526I	ACGT	3 cut(s)	480, 657, 1391
Hsp92I	GRCGYC	5 cut(s)	156, 372, 851, 1118, 1379
Hsp92II	CATG	8 cut(s)	328, 743, 1061, 1082, 1163, 1285, 1510, 1517
HspAI	GCGC	5 cut(s)	372, 851, 923, 1083, 1565
KasI	GGCGCC	2 cut(s)	371, 850
Kpn2I	TCCGGA	1 cut(s)	1672
KpnI	GGTACC	1 cut(s)	1561
KroI	GCCGGC	3 cut(s)	200, 847, 904
KroNI	GCCGGC	3 cut(s)	202, 849, 906
Ksp22I	TGATCA	1 cut(s)	1750
Kzo9I	GATC	6 cut(s)	504, 564, 667, 1510, 1750, 1866
LguI	GCTCTTC	2 cut(s)	545, 974
LmnI	GCTCC	5 cut(s)	140, 209, 371, 756, 1838
Lsp1109I	GCAGC	6 cut(s)	375, 831, 1251, 1426, 1757, 1989
LweI	GCATC	3 cut(s)	1408, 1597, 1732
MaeII	ACGT	3 cut(s)	480, 657, 1391
MaeIII	GTNAC	2 cut(s)	1331, 1679
MalI	GATC	6 cut(s)	506, 566, 669, 1512, 1752, 1868
MbiI	CCGCTC	1 cut(s)	555
MboI	GATC	6 cut(s)	504, 564, 667, 1510, 1750, 1866
MfeI	CAATTG	1 cut(s)	1659
MflI	RGATCY	1 cut(s)	1866
MhlI	GDGCHC	1 cut(s)	1526
MluCI	AATT	8 cut(s)	431, 751, 1219, 1465, 1637, 1659, 1668, 1693
Mly113I	GGCGCC	2 cut(s)	372, 851
MlyI	GAGTC	1 cut(s)	239
MmeI	TCCRAC	4 cut(s)	273, 318, 486, 1320
Mph1103I	ATGCAT	1 cut(s)	1401
MroI	TCCGGA	1 cut(s)	1672
MroNI	GCCGGC	3 cut(s)	200, 847, 904
MseI	TTAA	4 cut(s)	606, 987, 1437, 1908
MslI	CAYNNNNRTG	1 cut(s)	1158
MspA1I	CMGCKG	3 cut(s)	1544, 1748, 1895
MspCI	CTTAAG	1 cut(s)	605
MspI	CCGG	8 cut(s)	201, 315, 369, 848, 854, 900, 905, 1673
MspR9I	CCNGG	4 cut(s)	380, 574, 901, 1150
MunI	CAATTG	1 cut(s)	1659
Mva1269I	GAATGC	2 cut(s)	1153, 1720
MvaI	CCWGG	3 cut(s)	380, 574, 1150
MvnI	CGCG	2 cut(s)	174, 629
NaeI	GCCGGC	3 cut(s)	202, 849, 906
NarI	GGCGCC	2 cut(s)	372, 851
NciI	CCSGG	1 cut(s)	901
NdeII	GATC	6 cut(s)	504, 564, 667, 1510, 1750, 1866
NgoMIV	GCCGGC	3 cut(s)	200, 847, 904
NlaIII	CATG	8 cut(s)	328, 743, 1061, 1082, 1163, 1285, 1510, 1517
NlaIV	GGNNCC	5 cut(s)	136, 205, 373, 852, 1559
NmeAIII	GCCGAG	5 cut(s)	103, 111, 339, 1035, 1926
NmuCI	GTSAC	1 cut(s)	1679
NsiI	ATGCAT	1 cut(s)	1401
NspI	RCATGY	1 cut(s)	328
PagI	TCATGA	3 cut(s)	739, 1159, 1513
PceI	AGGCCT	1 cut(s)	1843
PciSI	GCTCTTC	2 cut(s)	545, 974
PcsI	WCGNNNNNNNCGW	2 cut(s)	477, 486
PctI	GAATGC	2 cut(s)	1153, 1720
PdiI	GCCGGC	3 cut(s)	202, 849, 906
PfeI	GAWTC	1 cut(s)	1423
PleI	GAGTC	1 cut(s)	239
PluTI	GGCGCC	2 cut(s)	375, 854
PpsI	GAGTC	1 cut(s)	239
Psp6I	CCWGG	3 cut(s)	378, 572, 1148
PspFI	CCCAGC	4 cut(s)	877, 883, 889, 1763
PspGI	CCWGG	3 cut(s)	378, 572, 1148
PspN4I	GGNNCC	5 cut(s)	136, 205, 373, 852, 1559
PspPI	GGNCC	4 cut(s)	789, 902, 1007, 1146
PsrI	GAACNNNNNNTAC	2 cut(s)	689, 721
PsuI	RGATCY	1 cut(s)	1866
PvuII	CAGCTG	3 cut(s)	1544, 1748, 1895
RsaI	GTAC	3 cut(s)	614, 1100, 1559
RsaNI	GTAC	3 cut(s)	613, 1099, 1558
RseI	CAYNNNNRTG	1 cut(s)	1158
SapI	GCTCTTC	2 cut(s)	545, 974
SaqAI	TTAA	4 cut(s)	606, 987, 1437, 1908
Sau3AI	GATC	6 cut(s)	504, 564, 667, 1510, 1750, 1866
Sau96I	GGNCC	4 cut(s)	789, 902, 1007, 1146
SchI	GAGTC	1 cut(s)	239
ScrFI	CCNGG	4 cut(s)	380, 574, 901, 1150
SduI	GDGCHC	1 cut(s)	1526
SfaNI	GCATC	3 cut(s)	1408, 1597, 1732
SfcI	CTRYAG	3 cut(s)	1024, 1044, 1862
SfoI	GGCGCC	2 cut(s)	373, 852
SinI	GGWCC	1 cut(s)	1146
SmiMI	CAYNNNNRTG	1 cut(s)	1158
SmlI	CTYRAG	4 cut(s)	605, 1207, 1298, 1870
SmoI	CTYRAG	4 cut(s)	605, 1207, 1298, 1870
Sse9I	AATT	8 cut(s)	431, 751, 1219, 1465, 1637, 1659, 1668, 1693
SseBI	AGGCCT	1 cut(s)	1843
SspDI	GGCGCC	2 cut(s)	371, 850
StuI	AGGCCT	1 cut(s)	1843
StyD4I	CCNGG	4 cut(s)	378, 572, 899, 1148
StyI	CCWWGG	1 cut(s)	839
TaaI	ACNGT	1 cut(s)	1096
TaiI	ACGT	3 cut(s)	483, 660, 1394
TaqI	TCGA	6 cut(s)	93, 237, 429, 435, 489, 495
TaqII	GACCGA	1 cut(s)	473
TasI	AATT	8 cut(s)	431, 751, 1219, 1465, 1637, 1659, 1668, 1693
TatI	WGTACW	1 cut(s)	1098
TauI	GCSGC	6 cut(s)	60, 74, 77, 877, 1043, 1136
TfiI	GAWTC	1 cut(s)	1423
Tru1I	TTAA	4 cut(s)	606, 987, 1437, 1908
Tru9I	TTAA	4 cut(s)	606, 987, 1437, 1908
TseFI	GTSAC	1 cut(s)	1679
TseI	GCWGC	6 cut(s)	363, 844, 1264, 1414, 1745, 1977
Tsp45I	GTSAC	1 cut(s)	1679
TspDTI	ATGAA	7 cut(s)	17, 409, 728, 1046, 1176, 1270, 1415
Vha464I	CTTAAG	1 cut(s)	605
VpaK11BI	GGWCC	1 cut(s)	1146
XapI	RAATTY	5 cut(s)	431, 1219, 1465, 1668, 1693
XceI	RCATGY	1 cut(s)	328
Zsp2I	ATGCAT	1 cut(s)	1401

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.