AT5G58490

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003662
Physical Location & Seq
Forward (+)
23643009 .. 23644679
1671 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G58490.1

Sequence Viewer

Length: 975 bp
ATGTTAACGGACGAGAGAGAAGTAGTCTGTGTCACCGGCGCCAGTGGCTGCATCGGCTCGTGGCTGGTCCATCAGCTCCTCCTCCGCGGCTACTCCGTCCACGCCACCGTGAAAAACCTCCAGGATGAGAAAGAGACGAAACATCTAGAAGGTCTCGAAGGTGCAGCCACACGCCTCCATCTATTCGAGATGGATCTCCTACAATACGACACCGTTTCCGCCGCCATCAATGGTTGCTCCGGCGTATTCCACCTCGCATCACCTTGTATCGTCGATGAAGTCCAAGATCCCCAGAAGCAACTACTTGACCCGGCGGTTAAAGGAACCATAAATGTTCTGACGGCGGCAAAAGAAGCCAGTGTTAAGAGAGTTGTTGTGACGTCTTCGATATCGGCGATTACTCCAAGTCCCAACTGGCCTGCTGATAAGATCAAGAATGAGGAATGTTGGGCTGCTGAAGACTACTGCAGGCAAAATGGATTGTGGTATCCACTGTCGAAGACGCTTGCTGAGAAGGCAGCTTGGGAATTTGCAGAGGAGAAAGGATTGGATGTGGTTGTGGTGAATCCAGGCACTGTCATGGGGCCTGTGATTCCTCCGTCTCTTAACGCTAGCATGCACATGCTTCTACGCCTTCTTCAGGGGTGCACGGAGACATACGAGAACTTCTTTATGGGGTCTGTGCATTTCAAGGATGTGGCTTTAGCTCATATTCTTGTATACGAGGATCCATATTCGAAAGGAAGGCACTTGTGCGTTGAGGCTATCTCTCACTACGGTGATTTTGTAGCCAAAGTTGCTGAGCTCTATCCCAATTACAATGTCCCCAAGTTACCGAGAGAGACTCAACCGGGTTTACTTCGAGATAAGAATGCATCAAAGAAGCTCATAGATTTGGGGTTAAAGTTCATTTCCATGGAGGAAATCATCAAGGAAGGTGTGGAGAGTCTTAAAAGCAAAGGATTTATCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

324

Amino Acids

35.76

Weight (kDa)

5.62

Isoelectric Point (pI)

31.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 142 1.3e-08 NmrA-like family
Epimerase PF01370 9 - 246 1.3e-26 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 9 - 152 7.7e-10 Polysaccharide biosynthesis protein
KR PF08659 9 - 136 4.9e-08 KR domain
3Beta_HSD PF01073 11 - 240 1.1e-20 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 129 3.2e-15 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 214 9.4e-13 Male sterility protein
NAD_binding_10 PF13460 13 - 192 1e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 383
AccB1I GGYRCC 1 cut(s) 38
AccI GTMKAC 1 cut(s) 720
AccII CGCG 1 cut(s) 87
AciI CCGC 6 cut(s) 85, 87, 219, 222, 314, 344
AclWI GGATC 4 cut(s) 201, 281, 722, 735
AcsI RAATTY 1 cut(s) 527
AcuI CTGAAG 2 cut(s) 477, 623
AcyI GRCGYC 2 cut(s) 39, 380
AfiI CCNNNNNNNGG 1 cut(s) 640
AgsI TTSAA 1 cut(s) 691
AjnI CCWGG 2 cut(s) 120, 568
AleI CACNNNNGTG 1 cut(s) 777
AluBI AGCT 5 cut(s) 76, 521, 707, 805, 886
AluI AGCT 5 cut(s) 76, 521, 707, 805, 886
Alw21I GWGCWC 2 cut(s) 650, 807
Alw26I GTCTC 5 cut(s) 128, 158, 606, 647, 836
Alw44I GTGCAC 1 cut(s) 646
AlwI GGATC 4 cut(s) 201, 281, 722, 735
AlwNI CAGNNNCTG 2 cut(s) 48, 575
AoxI GGCC 2 cut(s) 416, 584
ApaLI GTGCAC 1 cut(s) 646
ApeKI GCWGC 4 cut(s) 48, 164, 452, 518
ApoI RAATTY 1 cut(s) 527
AspLEI GCGC 1 cut(s) 41
AspS9I GGNCC 2 cut(s) 67, 584
AsuC2I CCSGG 2 cut(s) 311, 852
AsuHPI GGTGA 4 cut(s) 25, 252, 574, 791
AsuII TTCGAA 1 cut(s) 737
AsuNHI GCTAGC 1 cut(s) 611
AvaII GGWCC 1 cut(s) 67
BaeGI GKGCMC 1 cut(s) 650
BamHI GGATCC 1 cut(s) 727
BanI GGYRCC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 807
BauI CACGAG 1 cut(s) 58
BbsI GAAGAC 3 cut(s) 375, 465, 506
Bbv12I GWGCWC 2 cut(s) 650, 807
BbvI GCAGC 4 cut(s) 35, 176, 439, 530
BccI CCATC 4 cut(s) 78, 184, 186, 233
BceAI ACGGC 1 cut(s) 357
BciT130I CCWGG 2 cut(s) 122, 570
BciVI GTATCC 1 cut(s) 498
BcnI CCSGG 2 cut(s) 311, 852
BcoDI GTCTC 5 cut(s) 128, 158, 606, 647, 836
BfaI CTAG 2 cut(s) 146, 612
BfmI CTRYAG 1 cut(s) 466
BfoI RGCGCY 1 cut(s) 42
BfuI GTATCC 1 cut(s) 498
BisI GCNGC 7 cut(s) 49, 88, 165, 222, 345, 453, 519
BlpI GCTNAGC 1 cut(s) 801
BlsI GCNGC 7 cut(s) 50, 89, 166, 223, 346, 454, 520
Bme1390I CCNGG 4 cut(s) 122, 311, 570, 852
Bme18I GGWCC 1 cut(s) 67
BmgT120I GGNCC 2 cut(s) 67, 584
BmiI GGNNCC 4 cut(s) 40, 325, 585, 729
BmrFI CCNGG 4 cut(s) 122, 311, 570, 852
BmsI GCATC 3 cut(s) 60, 266, 884
BmtI GCTAGC 1 cut(s) 615
BpiI GAAGAC 3 cut(s) 375, 465, 506
BplI GAGNNNNNCTC 4 cut(s) 752, 784, 829, 861
BpmI CTGGAG 1 cut(s) 104
Bpu1102I GCTNAGC 1 cut(s) 801
Bpu14I TTCGAA 1 cut(s) 737
BpuMI CCSGG 2 cut(s) 311, 852
BsaHI GRCGYC 2 cut(s) 39, 380
BsaI GGTCTC 1 cut(s) 158
BsaJI CCNNGG 2 cut(s) 85, 915
Bsc4I CCNNNNNNNGG 1 cut(s) 640
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 3 cut(s) 42, 357, 419
BseBI CCWGG 2 cut(s) 122, 570
BseDI CCNNGG 2 cut(s) 85, 915
BseGI GGATG 3 cut(s) 130, 556, 700
BseLI CCNNNNNNNGG 1 cut(s) 640
BseMII CTCAG 2 cut(s) 501, 792
BseNI ACTGG 3 cut(s) 42, 357, 419
BseRI GAGGAG 3 cut(s) 68, 71, 551
BseSI GKGCMC 1 cut(s) 650
BseXI GCAGC 4 cut(s) 35, 176, 439, 530
BsgI GTGCAG 1 cut(s) 183
Bsh1236I CGCG 1 cut(s) 87
BshFI GGCC 2 cut(s) 418, 586
BshNI GGYRCC 1 cut(s) 38
BsiHKAI GWGCWC 2 cut(s) 650, 807
BsiSI CCGG 4 cut(s) 36, 240, 311, 851
BslFI GGGAC 2 cut(s) 393, 809
BslI CCNNNNNNNGG 1 cut(s) 640
BsmAI GTCTC 5 cut(s) 128, 158, 606, 647, 836
BsmBI CGTCTC 2 cut(s) 128, 606
BsmFI GGGAC 2 cut(s) 393, 809
BsmI GAATGC 1 cut(s) 877
BsnI GGCC 2 cut(s) 418, 586
Bso31I GGTCTC 1 cut(s) 158
Bsp119I TTCGAA 1 cut(s) 737
Bsp1286I GDGCHC 2 cut(s) 650, 807
Bsp143I GATC 4 cut(s) 193, 286, 429, 727
Bsp1720I GCTNAGC 1 cut(s) 801
Bsp19I CCATGG 1 cut(s) 915
BspACI CCGC 6 cut(s) 85, 87, 219, 222, 314, 344
BspANI GGCC 2 cut(s) 418, 586
BspCNI CTCAG 2 cut(s) 502, 793
BspFNI CGCG 1 cut(s) 87
BspLI GGNNCC 4 cut(s) 40, 325, 585, 729
BspMAI CTGCAG 1 cut(s) 470
BspOI GCTAGC 1 cut(s) 615
BspPI GGATC 4 cut(s) 201, 281, 722, 735
BspT104I TTCGAA 1 cut(s) 737
BspT107I GGYRCC 1 cut(s) 38
BspTNI GGTCTC 1 cut(s) 158
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 3 cut(s) 42, 357, 419
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 2 cut(s) 85, 915
BssMI GATC 4 cut(s) 193, 286, 429, 727
BssNAI GTATAC 1 cut(s) 721
BssNI GRCGYC 2 cut(s) 39, 380
BssSI CACGAG 1 cut(s) 58
BssT1I CCWWGG 1 cut(s) 915
Bst1107I GTATAC 1 cut(s) 721
Bst2BI CACGAG 1 cut(s) 58
Bst2UI CCWGG 2 cut(s) 122, 570
Bst4CI ACNGT 5 cut(s) 109, 214, 495, 577, 779
BstACI GRCGYC 2 cut(s) 39, 380
BstBI TTCGAA 1 cut(s) 737
BstC8I GCNNGC 5 cut(s) 420, 470, 507, 613, 617
BstDEI CTNAG 2 cut(s) 510, 801
BstDSI CCRYGG 2 cut(s) 85, 915
BstENI CCTNNNNNAGG 1 cut(s) 638
BstF5I GGATG 3 cut(s) 130, 556, 700
BstFNI CGCG 1 cut(s) 87
BstH2I RGCGCY 1 cut(s) 42
BstHHI GCGC 1 cut(s) 41
BstKTI GATC 4 cut(s) 196, 289, 432, 730
BstMAI GTCTC 5 cut(s) 128, 158, 606, 647, 836
BstMBI GATC 4 cut(s) 193, 286, 429, 727
BstMWI GCNNNNNNNGC 5 cut(s) 45, 54, 353, 515, 797
BstNI CCWGG 2 cut(s) 122, 570
BstNSI RCATGY 2 cut(s) 619, 625
BstSCI CCNGG 4 cut(s) 120, 309, 568, 850
BstSFI CTRYAG 1 cut(s) 466
BstSLI GKGCMC 1 cut(s) 650
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 4 cut(s) 35, 176, 439, 530
BstV2I GAAGAC 3 cut(s) 375, 465, 506
BstX2I RGATCY 3 cut(s) 193, 286, 727
BstYI RGATCY 3 cut(s) 193, 286, 727
BstZ17I GTATAC 1 cut(s) 721
BsuI GTATCC 1 cut(s) 498
BsuRI GGCC 2 cut(s) 418, 586
BtgI CCRYGG 2 cut(s) 85, 915
BtsCI GGATG 3 cut(s) 130, 556, 700
BtsIMutI CAGTG 4 cut(s) 49, 364, 491, 573
Cac8I GCNNGC 5 cut(s) 420, 470, 507, 613, 617
CaiI CAGNNNCTG 2 cut(s) 48, 575
CfoI GCGC 1 cut(s) 41
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 2 cut(s) 67, 584
Cfr42I CCGCGG 1 cut(s) 88
CseI GACGC 1 cut(s) 511
CviAII CATG 4 cut(s) 580, 616, 622, 916
DdeI CTNAG 2 cut(s) 510, 801
DinI GGCGCC 1 cut(s) 40
DpnI GATC 4 cut(s) 195, 288, 431, 729
DpnII GATC 4 cut(s) 193, 286, 429, 727
EciI GGCGGA 1 cut(s) 208
Ecl136II GAGCTC 1 cut(s) 805
Eco130I CCWWGG 1 cut(s) 915
Eco24I GRGCYC 1 cut(s) 807
Eco31I GGTCTC 1 cut(s) 158
Eco32I GATATC 1 cut(s) 390
Eco47I GGWCC 1 cut(s) 67
Eco53kI GAGCTC 1 cut(s) 805
Eco57I CTGAAG 2 cut(s) 477, 623
EcoICRI GAGCTC 1 cut(s) 805
EcoNI CCTNNNNNAGG 1 cut(s) 638
EcoO109I RGGNCCY 1 cut(s) 584
EcoRII CCWGG 2 cut(s) 120, 568
EcoRV GATATC 1 cut(s) 390
EcoT14I CCWWGG 1 cut(s) 915
EcoT22I ATGCAT 1 cut(s) 877
EcoT38I GRGCYC 1 cut(s) 807
EgeI GGCGCC 1 cut(s) 40
EheI GGCGCC 1 cut(s) 40
ErhI CCWWGG 1 cut(s) 915
Esp3I CGTCTC 2 cut(s) 128, 606
FaeI CATG 4 cut(s) 583, 619, 625, 919
FaqI GGGAC 2 cut(s) 393, 809
FatI CATG 4 cut(s) 579, 615, 621, 915
FblI GTMKAC 1 cut(s) 720
Fnu4HI GCNGC 7 cut(s) 49, 88, 165, 222, 345, 453, 519
FokI GGATG 3 cut(s) 137, 563, 707
FriOI GRGCYC 1 cut(s) 807
Fsp4HI GCNGC 7 cut(s) 49, 88, 165, 222, 345, 453, 519
FspBI CTAG 2 cut(s) 146, 612
GlaI GCGC 1 cut(s) 40
GluI GCNGC 7 cut(s) 49, 88, 165, 222, 345, 453, 519
GsuI CTGGAG 1 cut(s) 104
HaeII RGCGCY 1 cut(s) 42
HaeIII GGCC 2 cut(s) 418, 586
HapII CCGG 4 cut(s) 36, 240, 311, 851
HgaI GACGC 1 cut(s) 511
HhaI GCGC 1 cut(s) 41
Hin1I GRCGYC 2 cut(s) 39, 380
Hin1II CATG 4 cut(s) 583, 619, 625, 919
Hin6I GCGC 1 cut(s) 39
HinP1I GCGC 1 cut(s) 39
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 4 cut(s) 565, 592, 844, 946
HpaI GTTAAC 1 cut(s) 6
HpaII CCGG 4 cut(s) 36, 240, 311, 851
HphI GGTGA 4 cut(s) 25, 252, 574, 791
Hpy166II GTNNAC 5 cut(s) 6, 100, 648, 721, 857
Hpy188I TCNGA 1 cut(s) 339
Hpy188III TCNNGA 5 cut(s) 146, 155, 187, 433, 863
Hpy8I GTNNAC 5 cut(s) 6, 100, 648, 721, 857
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 6 cut(s) 143, 152, 508, 644, 738, 929
HpyCH4III ACNGT 5 cut(s) 109, 214, 495, 577, 779
HpyCH4IV ACGT 1 cut(s) 380
HpyCH4V TGCA 8 cut(s) 51, 164, 468, 533, 619, 648, 685, 875
HpyF10VI GCNNNNNNNGC 5 cut(s) 45, 54, 353, 515, 797
HpyF3I CTNAG 2 cut(s) 510, 801
HpySE526I ACGT 1 cut(s) 380
Hsp92I GRCGYC 2 cut(s) 39, 380
Hsp92II CATG 4 cut(s) 583, 619, 625, 919
HspAI GCGC 1 cut(s) 39
KasI GGCGCC 1 cut(s) 38
KspAI GTTAAC 1 cut(s) 6
KspI CCGCGG 1 cut(s) 88
Kzo9I GATC 4 cut(s) 193, 286, 429, 727
LmnI GCTCC 2 cut(s) 81, 242
Lsp1109I GCAGC 4 cut(s) 35, 176, 439, 530
LweI GCATC 3 cut(s) 60, 266, 884
MaeI CTAG 2 cut(s) 146, 612
MaeII ACGT 1 cut(s) 380
MaeIII GTNAC 3 cut(s) 31, 376, 831
MalI GATC 4 cut(s) 195, 288, 431, 729
MboI GATC 4 cut(s) 193, 286, 429, 727
MboII GAAGA 4 cut(s) 375, 470, 511, 629
MflI RGATCY 3 cut(s) 193, 286, 727
MhlI GDGCHC 2 cut(s) 650, 807
MluCI AATT 2 cut(s) 527, 814
Mly113I GGCGCC 1 cut(s) 39
MlyI GAGTC 2 cut(s) 838, 955
Mph1103I ATGCAT 1 cut(s) 877
MseI TTAA 7 cut(s) 5, 318, 363, 606, 902, 951, 973
MslI CAYNNNNRTG 4 cut(s) 578, 620, 777, 914
MspA1I CMGCKG 1 cut(s) 87
MspI CCGG 4 cut(s) 36, 240, 311, 851
MspR9I CCNGG 4 cut(s) 122, 311, 570, 852
Mva1269I GAATGC 1 cut(s) 877
MvaI CCWGG 2 cut(s) 122, 570
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 5 cut(s) 45, 54, 353, 515, 797
NarI GGCGCC 1 cut(s) 39
NciI CCSGG 2 cut(s) 311, 852
NcoI CCATGG 1 cut(s) 915
NdeII GATC 4 cut(s) 193, 286, 429, 727
NheI GCTAGC 1 cut(s) 611
NlaIII CATG 4 cut(s) 583, 619, 625, 919
NlaIV GGNNCC 4 cut(s) 40, 325, 585, 729
NmuCI GTSAC 2 cut(s) 31, 376
NsiI ATGCAT 1 cut(s) 877
NspI RCATGY 2 cut(s) 619, 625
NspV TTCGAA 1 cut(s) 737
OliI CACNNNNGTG 1 cut(s) 777
PaeI GCATGC 1 cut(s) 619
PcsI WCGNNNNNNNCGW 1 cut(s) 392
PctI GAATGC 1 cut(s) 877
PfeI GAWTC 2 cut(s) 565, 592
PfoI TCCNGGA 1 cut(s) 120
PkrI GCNGC 7 cut(s) 50, 89, 166, 223, 346, 454, 520
PleI GAGTC 2 cut(s) 838, 954
PluTI GGCGCC 1 cut(s) 42
PpsI GAGTC 2 cut(s) 838, 954
Psp124BI GAGCTC 1 cut(s) 807
Psp6I CCWGG 2 cut(s) 120, 568
PspGI CCWGG 2 cut(s) 120, 568
PspN4I GGNNCC 4 cut(s) 40, 325, 585, 729
PspPI GGNCC 2 cut(s) 67, 584
PstI CTGCAG 1 cut(s) 470
PstNI CAGNNNCTG 2 cut(s) 48, 575
PsuI RGATCY 3 cut(s) 193, 286, 727
RseI CAYNNNNRTG 4 cut(s) 578, 620, 777, 914
SacI GAGCTC 1 cut(s) 807
SacII CCGCGG 1 cut(s) 88
SaqAI TTAA 7 cut(s) 5, 318, 363, 606, 902, 951, 973
SatI GCNGC 7 cut(s) 49, 88, 165, 222, 345, 453, 519
Sau3AI GATC 4 cut(s) 193, 286, 429, 727
Sau96I GGNCC 2 cut(s) 67, 584
SchI GAGTC 2 cut(s) 838, 955
ScrFI CCNGG 4 cut(s) 122, 311, 570, 852
SduI GDGCHC 2 cut(s) 650, 807
SfaNI GCATC 3 cut(s) 60, 266, 884
SfcI CTRYAG 1 cut(s) 466
SfoI GGCGCC 1 cut(s) 40
Sfr303I CCGCGG 1 cut(s) 88
SfuI TTCGAA 1 cut(s) 737
SgrAI CRCCGGYG 1 cut(s) 35
SgrBI CCGCGG 1 cut(s) 88
SinI GGWCC 1 cut(s) 67
SmiMI CAYNNNNRTG 4 cut(s) 578, 620, 777, 914
SphI GCATGC 1 cut(s) 619
Sse9I AATT 2 cut(s) 527, 814
SsiI CCGC 6 cut(s) 85, 87, 219, 222, 314, 344
SspDI GGCGCC 1 cut(s) 38
SspMI CTAG 2 cut(s) 146, 612
SstI GAGCTC 1 cut(s) 807
StyD4I CCNGG 4 cut(s) 120, 309, 568, 850
StyI CCWWGG 1 cut(s) 915
TaaI ACNGT 5 cut(s) 109, 214, 495, 577, 779
TaiI ACGT 1 cut(s) 383
TaqI TCGA 7 cut(s) 156, 186, 273, 386, 497, 737, 862
TasI AATT 2 cut(s) 527, 814
TauI GCSGC 3 cut(s) 90, 224, 347
TfiI GAWTC 2 cut(s) 565, 592
Tru1I TTAA 7 cut(s) 5, 318, 363, 606, 902, 951, 973
Tru9I TTAA 7 cut(s) 5, 318, 363, 606, 902, 951, 973
TscAI CASTG 4 cut(s) 49, 364, 498, 580
TseFI GTSAC 2 cut(s) 31, 376
TseI GCWGC 4 cut(s) 48, 164, 452, 518
Tsp45I GTSAC 2 cut(s) 31, 376
TspDTI ATGAA 2 cut(s) 291, 898
TspGWI ACGGA 4 cut(s) 23, 85, 588, 665
TspRI CASTG 4 cut(s) 49, 364, 498, 580
VneI GTGCAC 1 cut(s) 646
VpaK11BI GGWCC 1 cut(s) 67
XagI CCTNNNNNAGG 1 cut(s) 638
XapI RAATTY 1 cut(s) 527
XbaI TCTAGA 1 cut(s) 145
XceI RCATGY 2 cut(s) 619, 625
XcmI CCANNNNNNNNNTGG 1 cut(s) 411
XmiI GTMKAC 1 cut(s) 720
XspI CTAG 2 cut(s) 146, 612
ZraI GACGTC 1 cut(s) 381
Zsp2I ATGCAT 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.