Rroxscaffold_1G00009940

cinnamoyl-CoA reductase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003662
Physical Location & Seq
Forward (+)
12425410 .. 12428349
2940 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00009940.1

Sequence Viewer

Length: 993 bp
ATGTCGAAGCAGGGTGAGGTCGTATGCGTCACCGGTGGAAGCGGCTGCATCGGATCATGGGTCGTCCGCCTCCTCCTCGACCGCGGCTACACCGTCCACGCCACCGTCAAAGACCTCAAGGACGACGGCGAGACGAAGCATCTAGAAGCCCTAGAAGGAGCCCAAACGCGCCTCCGCCTCTTCCAGATCGACCTCCTCGACTACGATTCCATCTCCGCCGCAGTCCACGGCTGCTCCGGCGTCTTCCACCTTGCCTCTCCTTGCATCGTTGACCAAGTCGATGACCCCCAGAAGGAGCTGTTGGACCCGGCGATTAAAGGAACGCTGAATGTTCTAACGGCGGCTAAGCAGGCCGGCGTCAAGCGCGTGGTGGTCACGTCTTCCGTCTCGGCCATCACTCCCAGCCCTACTTGGCCGGCCGATGTCGTCAAGAGAGAGGATTGCTGGACTGATGTTGAGTACTGCAAGCAGAAAGGACTGTGGTATCCTTTATCGAAAACTCTGGCGGAGAAAGCTGCTTGGGAATTTGCCAAGGAGAAGGGGTTGGATGTGGTTGTGGTGAATCCGGGGACGGTGATGGGTGATGTTATATCGCCGAGGCTCAATGCTAGCATGTTGTTGCTCGTACGCCTTCTTGAAGGCTGCACTGAAACATATGAGAACTTCTTTATGGGATCTGTGCATTTTAAAGATGTAGCTCTAGCACACGTTTTAGTTTACGAGAACAAATCGGCCACTGGTAGGCACATGTGCGTTGAGGCAATATCACATTATGGTGATTTTGTAGCAAAGGTAGCTGAACTTTATCCCGAGTACAAGATTCCCAGTAACTTCTATTCTGAAACTGGTTTGCCAAAGGACACACAGCCTGGGTTGTTGAGGGAAAAGACTGGAGCGAAAAAGCTAATGGACTTGGGTATAGACTTCATCCCCATGGATCAAATTATCAAGGATGCTGTTGAGTGCCTAAAGAGCAAGGGATTTATTTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

330

Amino Acids

36.17

Weight (kDa)

5.67

Isoelectric Point (pI)

21.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 131 2.2e-13 NmrA-like family
Epimerase PF01370 8 - 245 3.1e-26 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 8 - 135 8.8e-10 Polysaccharide biosynthesis protein
KR PF08659 8 - 135 8.8e-09 KR domain
3Beta_HSD PF01073 10 - 240 1.8e-20 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 128 3.2e-17 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 205 1.1e-09 Male sterility protein
NAD_binding_10 PF13460 12 - 192 6.3e-15 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 84, 169, 366
AciI CCGC 9 cut(s) 42, 67, 82, 84, 175, 216, 219, 341, 506
AclWI GGATC 3 cut(s) 61, 682, 945
AcoI YGGCCR 4 cut(s) 390, 413, 417, 732
AcsI RAATTY 1 cut(s) 524
AcyI GRCGYC 2 cut(s) 240, 357
AfaI GTAC 3 cut(s) 461, 627, 815
AfiI CCNNNNNNNGG 2 cut(s) 292, 741
AflIII ACRYGT 2 cut(s) 706, 747
AgeI ACCGGT 1 cut(s) 32
AgsI TTSAA 1 cut(s) 638
AjiI CACGTC 1 cut(s) 378
AjnI CCWGG 1 cut(s) 868
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
AluBI AGCT 5 cut(s) 298, 515, 698, 797, 904
AluI AGCT 5 cut(s) 298, 515, 698, 797, 904
Alw26I GTCTC 2 cut(s) 125, 391
AlwI GGATC 3 cut(s) 61, 682, 945
Ama87I CYCGRG 1 cut(s) 809
AoxI GGCC 5 cut(s) 351, 390, 413, 417, 732
ApeKI GCWGC 4 cut(s) 45, 231, 515, 642
ApoI RAATTY 1 cut(s) 524
AsiGI ACCGGT 1 cut(s) 32
AspLEI GCGC 2 cut(s) 171, 366
AspS9I GGNCC 1 cut(s) 304
AsuC2I CCSGG 2 cut(s) 308, 567
AsuHPI GGTGA 6 cut(s) 22, 26, 571, 586, 593, 788
AsuNHI GCTAGC 1 cut(s) 608
AvaI CYCGRG 1 cut(s) 809
AvaII GGWCC 1 cut(s) 304
BaeI ACNNNNGTAYC 2 cut(s) 467, 500
BanII GRGCYC 1 cut(s) 163
BbsI GAAGAC 2 cut(s) 235, 372
BbvI GCAGC 4 cut(s) 32, 218, 502, 629
BccI CCATC 3 cut(s) 218, 401, 571
BceAI ACGGC 3 cut(s) 142, 244, 354
BciT130I CCWGG 1 cut(s) 870
BciVI GTATCC 1 cut(s) 495
BcnI CCSGG 2 cut(s) 308, 567
BcoDI GTCTC 2 cut(s) 125, 391
BfaI CTAG 4 cut(s) 143, 152, 609, 701
BfuI GTATCC 1 cut(s) 495
BisI GCNGC 8 cut(s) 43, 46, 85, 219, 232, 342, 516, 643
BlpI GCTNAGC 1 cut(s) 345
BlsI GCNGC 8 cut(s) 44, 47, 86, 220, 233, 343, 517, 644
BmcAI AGTACT 1 cut(s) 461
Bme1390I CCNGG 3 cut(s) 308, 567, 870
Bme18I GGWCC 1 cut(s) 304
BmeT110I CYCGRG 1 cut(s) 809
BmgBI CACGTC 1 cut(s) 378
BmgT120I GGNCC 1 cut(s) 304
BmiI GGNNCC 2 cut(s) 160, 306
BmrFI CCNGG 3 cut(s) 308, 567, 870
BmrI ACTGGG 1 cut(s) 819
BmsI GCATC 4 cut(s) 57, 148, 273, 943
BmtI GCTAGC 1 cut(s) 612
BmuI ACTGGG 1 cut(s) 819
BpiI GAAGAC 2 cut(s) 235, 372
BpmI CTGGAG 1 cut(s) 912
Bpu1102I GCTNAGC 1 cut(s) 345
BpuEI CTTGAG 1 cut(s) 101
BpuMI CCSGG 2 cut(s) 308, 567
BsaHI GRCGYC 2 cut(s) 240, 357
BsaJI CCNNGG 7 cut(s) 82, 226, 531, 566, 596, 869, 933
BsaWI WCCGGW 1 cut(s) 32
BsaXI ACNNNNNCTCC 4 cut(s) 218, 248, 527, 557
Bsc4I CCNNNNNNNGG 2 cut(s) 292, 741
Bse118I RCCGGY 3 cut(s) 32, 353, 415
Bse1I ACTGG 4 cut(s) 742, 825, 850, 895
BseBI CCWGG 1 cut(s) 870
BseDI CCNNGG 7 cut(s) 82, 226, 531, 566, 596, 869, 933
BseGI GGATG 3 cut(s) 553, 927, 958
BseLI CCNNNNNNNGG 2 cut(s) 292, 741
BseNI ACTGG 4 cut(s) 742, 825, 850, 895
BseRI GAGGAG 3 cut(s) 62, 65, 185
BseX3I CGGCCG 1 cut(s) 417
BseXI GCAGC 4 cut(s) 32, 218, 502, 629
BseYI CCCAGC 1 cut(s) 401
BsgI GTGCAG 1 cut(s) 628
Bsh1236I CGCG 3 cut(s) 84, 169, 366
Bsh1285I CGRYCG 2 cut(s) 82, 420
BshFI GGCC 5 cut(s) 353, 392, 415, 419, 734
BshTI ACCGGT 1 cut(s) 32
BsiEI CGRYCG 2 cut(s) 82, 420
BsiHKCI CYCGRG 1 cut(s) 809
BsiSI CCGG 6 cut(s) 33, 237, 308, 354, 416, 566
BsiWI CGTACG 1 cut(s) 625
BslFI GGGAC 1 cut(s) 583
BslI CCNNNNNNNGG 2 cut(s) 292, 741
BsmAI GTCTC 2 cut(s) 125, 391
BsmBI CGTCTC 2 cut(s) 125, 391
BsmFI GGGAC 1 cut(s) 583
BsnI GGCC 5 cut(s) 353, 392, 415, 419, 734
BsoBI CYCGRG 1 cut(s) 809
Bsp1286I GDGCHC 1 cut(s) 163
Bsp143I GATC 4 cut(s) 53, 186, 674, 937
Bsp1720I GCTNAGC 1 cut(s) 345
Bsp19I CCATGG 1 cut(s) 933
BspACI CCGC 9 cut(s) 42, 67, 82, 84, 175, 216, 219, 341, 506
BspANI GGCC 5 cut(s) 353, 392, 415, 419, 734
BspFNI CGCG 3 cut(s) 84, 169, 366
BspLI GGNNCC 2 cut(s) 160, 306
BspOI GCTAGC 1 cut(s) 612
BspPI GGATC 3 cut(s) 61, 682, 945
BsrFI RCCGGY 3 cut(s) 32, 353, 415
BsrI ACTGG 4 cut(s) 742, 825, 850, 895
BssAI RCCGGY 3 cut(s) 32, 353, 415
BssECI CCNNGG 7 cut(s) 82, 226, 531, 566, 596, 869, 933
BssMI GATC 4 cut(s) 53, 186, 674, 937
BssNI GRCGYC 2 cut(s) 240, 357
BssT1I CCWWGG 2 cut(s) 531, 933
Bst2UI CCWGG 1 cut(s) 870
Bst4CI ACNGT 4 cut(s) 94, 106, 480, 574
Bst6I CTCTTC 1 cut(s) 185
BstACI GRCGYC 2 cut(s) 240, 357
BstC8I GCNNGC 5 cut(s) 351, 355, 417, 467, 610
BstDEI CTNAG 1 cut(s) 345
BstDSI CCRYGG 3 cut(s) 82, 226, 933
BstF5I GGATG 3 cut(s) 553, 927, 958
BstFNI CGCG 3 cut(s) 84, 169, 366
BstHHI GCGC 2 cut(s) 171, 366
BstKTI GATC 4 cut(s) 56, 189, 677, 940
BstMAI GTCTC 2 cut(s) 125, 391
BstMBI GATC 4 cut(s) 53, 186, 674, 937
BstMCI CGRYCG 2 cut(s) 82, 420
BstMWI GCNNNNNNNGC 6 cut(s) 237, 350, 363, 512, 794, 972
BstNI CCWGG 1 cut(s) 870
BstNSI RCATGY 2 cut(s) 616, 751
BstSCI CCNGG 3 cut(s) 306, 565, 868
BstUI CGCG 3 cut(s) 84, 169, 366
BstV1I GCAGC 4 cut(s) 32, 218, 502, 629
BstV2I GAAGAC 2 cut(s) 235, 372
BstX2I RGATCY 1 cut(s) 674
BstYI RGATCY 1 cut(s) 674
BstZI CGGCCG 1 cut(s) 417
BsuI GTATCC 1 cut(s) 495
BsuRI GGCC 5 cut(s) 353, 392, 415, 419, 734
BtgI CCRYGG 3 cut(s) 82, 226, 933
BtrI CACGTC 1 cut(s) 378
BtsCI GGATG 3 cut(s) 553, 927, 958
BtsIMutI CAGTG 2 cut(s) 645, 735
Cac8I GCNNGC 5 cut(s) 351, 355, 417, 467, 610
CfoI GCGC 2 cut(s) 171, 366
Cfr10I RCCGGY 3 cut(s) 32, 353, 415
Cfr13I GGNCC 1 cut(s) 304
Cfr42I CCGCGG 1 cut(s) 85
CseI GACGC 3 cut(s) 16, 229, 346
Csp6I GTAC 3 cut(s) 460, 626, 814
CspAI ACCGGT 1 cut(s) 32
CviAII CATG 4 cut(s) 57, 613, 748, 934
CviQI GTAC 3 cut(s) 460, 626, 814
DdeI CTNAG 1 cut(s) 345
DpnI GATC 4 cut(s) 55, 188, 676, 939
DpnII GATC 4 cut(s) 53, 186, 674, 937
DraI TTTAAA 1 cut(s) 688
EaeI YGGCCR 4 cut(s) 390, 413, 417, 732
EagI CGGCCG 1 cut(s) 417
Eam1104I CTCTTC 1 cut(s) 185
EarI CTCTTC 1 cut(s) 185
EciI GGCGGA 4 cut(s) 56, 164, 205, 521
EclXI CGGCCG 1 cut(s) 417
Eco130I CCWWGG 2 cut(s) 531, 933
Eco24I GRGCYC 1 cut(s) 163
Eco47I GGWCC 1 cut(s) 304
Eco52I CGGCCG 1 cut(s) 417
Eco88I CYCGRG 1 cut(s) 809
EcoRII CCWGG 1 cut(s) 868
EcoT14I CCWWGG 2 cut(s) 531, 933
EcoT38I GRGCYC 1 cut(s) 163
ErhI CCWWGG 2 cut(s) 531, 933
Esp3I CGTCTC 2 cut(s) 125, 391
FaeI CATG 4 cut(s) 60, 616, 751, 937
FaqI GGGAC 1 cut(s) 583
FatI CATG 4 cut(s) 56, 612, 747, 933
FauNDI CATATG 1 cut(s) 655
Fnu4HI GCNGC 8 cut(s) 43, 46, 85, 219, 232, 342, 516, 643
FokI GGATG 3 cut(s) 560, 914, 965
FriOI GRGCYC 1 cut(s) 163
FseI GGCCGGCC 1 cut(s) 419
Fsp4HI GCNGC 8 cut(s) 43, 46, 85, 219, 232, 342, 516, 643
FspBI CTAG 4 cut(s) 143, 152, 609, 701
GlaI GCGC 2 cut(s) 170, 365
GluI GCNGC 8 cut(s) 43, 46, 85, 219, 232, 342, 516, 643
GsaI CCCAGC 1 cut(s) 405
GsuI CTGGAG 1 cut(s) 912
HaeIII GGCC 5 cut(s) 353, 392, 415, 419, 734
HapII CCGG 6 cut(s) 33, 237, 308, 354, 416, 566
HgaI GACGC 3 cut(s) 16, 229, 346
HhaI GCGC 2 cut(s) 171, 366
Hin1I GRCGYC 2 cut(s) 240, 357
Hin1II CATG 4 cut(s) 60, 616, 751, 937
Hin6I GCGC 2 cut(s) 169, 364
HinP1I GCGC 2 cut(s) 169, 364
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 3 cut(s) 206, 562, 820
HpaII CCGG 6 cut(s) 33, 237, 308, 354, 416, 566
HphI GGTGA 6 cut(s) 22, 26, 571, 586, 593, 788
Hpy166II GTNNAC 4 cut(s) 97, 226, 271, 718
Hpy188I TCNGA 2 cut(s) 53, 841
Hpy188III TCNNGA 5 cut(s) 143, 184, 430, 635, 809
Hpy8I GTNNAC 4 cut(s) 97, 226, 271, 718
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 5 cut(s) 149, 286, 532, 632, 641
HpyCH4III ACNGT 4 cut(s) 94, 106, 480, 574
HpyCH4IV ACGT 2 cut(s) 377, 708
HpyCH4V TGCA 5 cut(s) 48, 264, 465, 645, 682
HpyF10VI GCNNNNNNNGC 6 cut(s) 237, 350, 363, 512, 794, 972
HpyF3I CTNAG 1 cut(s) 345
HpySE526I ACGT 2 cut(s) 377, 708
Hsp92I GRCGYC 2 cut(s) 240, 357
Hsp92II CATG 4 cut(s) 60, 616, 751, 937
HspAI GCGC 2 cut(s) 169, 364
KroI GCCGGC 2 cut(s) 353, 415
KroNI GCCGGC 2 cut(s) 355, 417
KspI CCGCGG 1 cut(s) 85
Kzo9I GATC 4 cut(s) 53, 186, 674, 937
LmnI GCTCC 4 cut(s) 158, 239, 295, 893
Lsp1109I GCAGC 4 cut(s) 32, 218, 502, 629
LweI GCATC 4 cut(s) 57, 148, 273, 943
MaeI CTAG 4 cut(s) 143, 152, 609, 701
MaeII ACGT 2 cut(s) 377, 708
MaeIII GTNAC 3 cut(s) 28, 373, 827
MalI GATC 4 cut(s) 55, 188, 676, 939
MboI GATC 4 cut(s) 53, 186, 674, 937
MboII GAAGA 3 cut(s) 172, 235, 372
MflI RGATCY 1 cut(s) 674
MhlI GDGCHC 1 cut(s) 163
MluCI AATT 2 cut(s) 524, 942
MmeI TCCRAC 2 cut(s) 282, 525
MroNI GCCGGC 2 cut(s) 353, 415
MseI TTAA 2 cut(s) 315, 687
MslI CAYNNNNRTG 2 cut(s) 774, 932
MspA1I CMGCKG 1 cut(s) 84
MspI CCGG 6 cut(s) 33, 237, 308, 354, 416, 566
MspR9I CCNGG 3 cut(s) 308, 567, 870
MvaI CCWGG 1 cut(s) 870
MvnI CGCG 3 cut(s) 84, 169, 366
MwoI GCNNNNNNNGC 6 cut(s) 237, 350, 363, 512, 794, 972
NaeI GCCGGC 2 cut(s) 355, 417
NciI CCSGG 2 cut(s) 308, 567
NcoI CCATGG 1 cut(s) 933
NdeI CATATG 1 cut(s) 655
NdeII GATC 4 cut(s) 53, 186, 674, 937
NgoMIV GCCGGC 2 cut(s) 353, 415
NheI GCTAGC 1 cut(s) 608
NlaIII CATG 4 cut(s) 60, 616, 751, 937
NlaIV GGNNCC 2 cut(s) 160, 306
NmeAIII GCCGAG 2 cut(s) 368, 621
NmuCI GTSAC 2 cut(s) 28, 373
NspI RCATGY 2 cut(s) 616, 751
PciI ACATGT 1 cut(s) 747
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PdiI GCCGGC 2 cut(s) 355, 417
PfeI GAWTC 3 cut(s) 206, 562, 820
Pfl23II CGTACG 1 cut(s) 625
PflFI GACNNNGTC 1 cut(s) 275
PinAI ACCGGT 1 cut(s) 32
PkrI GCNGC 8 cut(s) 44, 47, 86, 220, 233, 343, 517, 644
PscI ACATGT 1 cut(s) 747
Psp6I CCWGG 1 cut(s) 868
PspFI CCCAGC 1 cut(s) 401
PspGI CCWGG 1 cut(s) 868
PspLI CGTACG 1 cut(s) 625
PspN4I GGNNCC 2 cut(s) 160, 306
PspPI GGNCC 1 cut(s) 304
PsuI RGATCY 1 cut(s) 674
PsyI GACNNNGTC 1 cut(s) 275
RigI GGCCGGCC 1 cut(s) 419
RsaI GTAC 3 cut(s) 461, 627, 815
RsaNI GTAC 3 cut(s) 460, 626, 814
RseI CAYNNNNRTG 2 cut(s) 774, 932
SacII CCGCGG 1 cut(s) 85
SaqAI TTAA 2 cut(s) 315, 687
SatI GCNGC 8 cut(s) 43, 46, 85, 219, 232, 342, 516, 643
Sau3AI GATC 4 cut(s) 53, 186, 674, 937
Sau96I GGNCC 1 cut(s) 304
ScaI AGTACT 1 cut(s) 461
ScrFI CCNGG 3 cut(s) 308, 567, 870
SduI GDGCHC 1 cut(s) 163
SfaNI GCATC 4 cut(s) 57, 148, 273, 943
Sfr303I CCGCGG 1 cut(s) 85
SgrAI CRCCGGYG 1 cut(s) 32
SgrBI CCGCGG 1 cut(s) 85
SinI GGWCC 1 cut(s) 304
SmiMI CAYNNNNRTG 2 cut(s) 774, 932
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse9I AATT 2 cut(s) 524, 942
SsiI CCGC 9 cut(s) 42, 67, 82, 84, 175, 216, 219, 341, 506
SspMI CTAG 4 cut(s) 143, 152, 609, 701
StyD4I CCNGG 3 cut(s) 306, 565, 868
StyI CCWWGG 2 cut(s) 531, 933
TaaI ACNGT 4 cut(s) 94, 106, 480, 574
TaiI ACGT 2 cut(s) 380, 711
TaqI TCGA 6 cut(s) 5, 78, 189, 198, 279, 494
TasI AATT 2 cut(s) 524, 942
TatI WGTACW 2 cut(s) 459, 813
TauI GCSGC 4 cut(s) 45, 87, 221, 344
TfiI GAWTC 3 cut(s) 206, 562, 820
Tru1I TTAA 2 cut(s) 315, 687
Tru9I TTAA 2 cut(s) 315, 687
TscAI CASTG 2 cut(s) 652, 742
TseFI GTSAC 2 cut(s) 28, 373
TseI GCWGC 4 cut(s) 45, 231, 515, 642
Tsp45I GTSAC 2 cut(s) 28, 373
TspDTI ATGAA 1 cut(s) 916
TspGWI ACGGA 1 cut(s) 373
TspRI CASTG 2 cut(s) 652, 742
Tth111I GACNNNGTC 1 cut(s) 275
VpaK11BI GGWCC 1 cut(s) 304
XapI RAATTY 1 cut(s) 524
XbaI TCTAGA 1 cut(s) 142
XceI RCATGY 2 cut(s) 616, 751
XspI CTAG 4 cut(s) 143, 152, 609, 701
ZrmI AGTACT 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.