Rw4G017980

cinnamoyl-CoA reductase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003662
Physical Location & Seq
Reverse (-)
40153434 .. 40156930
3497 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G017980.1

Sequence Viewer

Length: 1011 bp
ATGTCGAAGCAGGGTGAGGTCGTATGCGTCACCGGTGGAAGCGGCTGCATCGGATCATGGGTTGTCCGCCTCCTCCTCGACCGCGGCTACACCGTCCACGCCACCGTCAAAGACCTTATCGACCTCCTCGACTACGATTCCATCTCCGCCGCAGTCCACGGCTGCTCCGGCGTCTTCCACCTCGCCTCTCCTTGCATCGTCGACCAAGTCCATGACCCCCAGAAGGAGCTGTTGGACCCGGTGATTAAAGGAACGCTGAATGTTCTAACGGCGGCTAAGCAGGCCTGCGTGAAGCGCGTGGTGCTCACGTCCTCCATATCGGCCATCACTCCCAGCCCTACTTGGCAGGCCGATGTCGTCAAGAGAGAGGATTGCTGGACTGATGTTGAGTACTGCAAGCAGAAAGGACTGTGGTATCCTTTATCGAAAACTCTGGCGGAGAAAGCTGCGTGGGAATTTGCCGAGGAGAAGGGGTTGGATGTGGTTGTGGTGAATCCGGGGACAGTGATGGGTGATGTTATATCGCCGAGGCTCAATGCTAGCAAAGGTGAGTGCTCTGTTCAGTTTCTCATGAAACCTTTCTTGCATTTGCCTAATTTTCATTTTACTTTTCTCTTGGTGGTGTCAAGTGAGATTGTTGGAGAAGAAGCTTCCACCACTTCTGGCCACATTATGCTACTTGAGGAACAACCCATTACTCATTCTTCTGCAAAAATGGAAGCTCAATCTCCAGGTATTTCAGCAATCCAGAGTTTCCTTATGTTTTTAGCTTTCAAGTTGGTGAACTGGTTGGGAAAAATTCGGCATCAAAACATCATCAAACTTTTGGGTTACTGTGTTCATGGAAAAACAAGGTTTCTTGTGTATGAAATGATGCAGAATGGCTCCTTACAAACTCAATTGTATGGTATGAACTTTCTATTGGTATCAAGTAACATAGATGGAGAAAGACATTTGCAAATGGCACAAGAAGGTTTTGTTGATGCGAAAAGATATTATATCAGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

336

Amino Acids

37.17

Weight (kDa)

6.26

Isoelectric Point (pI)

35.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 8 - 172 1.4e-20 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 40 - 149 1.8e-11 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 40 - 168 9.8e-06 NAD(P)H-binding
NAD_binding_4 PF07993 52 - 175 7.6e-08 Male sterility protein
PK_Tyr_Ser-Thr PF07714 261 - 312 1.3e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 201
AccII CGCG 2 cut(s) 84, 297
AciI CCGC 8 cut(s) 42, 67, 82, 84, 147, 150, 272, 437
AclWI GGATC 1 cut(s) 61
AcoI YGGCCR 2 cut(s) 321, 664
AcsI RAATTY 2 cut(s) 455, 798
AcyI GRCGYC 1 cut(s) 171
AfaI GTAC 1 cut(s) 392
AfiI CCNNNNNNNGG 1 cut(s) 223
AgeI ACCGGT 1 cut(s) 32
AgsI TTSAA 1 cut(s) 775
AjiI CACGTC 1 cut(s) 309
AjnI CCWGG 1 cut(s) 730
AjuI GAANNNNNNNTTGG 4 cut(s) 215, 247, 905, 937
AluBI AGCT 6 cut(s) 229, 446, 650, 722, 770, 1005
AluI AGCT 6 cut(s) 229, 446, 650, 722, 770, 1005
Alw21I GWGCWC 2 cut(s) 306, 557
AlwI GGATC 1 cut(s) 61
AoxI GGCC 4 cut(s) 282, 321, 348, 664
ApeKI GCWGC 3 cut(s) 45, 162, 446
ApoI RAATTY 2 cut(s) 455, 798
AsiGI ACCGGT 1 cut(s) 32
Asp700I GAANNNNTTC 1 cut(s) 578
AspLEI GCGC 1 cut(s) 297
AspS9I GGNCC 1 cut(s) 235
AsuC2I CCSGG 2 cut(s) 239, 498
AsuHPI GGTGA 7 cut(s) 22, 26, 253, 502, 524, 560, 793
AsuNHI GCTAGC 1 cut(s) 539
AvaII GGWCC 1 cut(s) 235
BaeI ACNNNNGTAYC 2 cut(s) 398, 431
BalI TGGCCA 1 cut(s) 666
BbsI GAAGAC 1 cut(s) 166
Bbv12I GWGCWC 2 cut(s) 306, 557
BbvI GCAGC 3 cut(s) 32, 149, 433
BccI CCATC 4 cut(s) 149, 332, 502, 935
BceAI ACGGC 2 cut(s) 175, 285
BciT130I CCWGG 1 cut(s) 732
BciVI GTATCC 1 cut(s) 426
BcnI CCSGG 2 cut(s) 239, 498
BfaI CTAG 1 cut(s) 540
BfuI GTATCC 1 cut(s) 426
BisI GCNGC 7 cut(s) 43, 46, 85, 150, 163, 273, 447
BlpI GCTNAGC 1 cut(s) 276
BlsI GCNGC 7 cut(s) 44, 47, 86, 151, 164, 274, 448
BmcAI AGTACT 1 cut(s) 392
Bme1390I CCNGG 3 cut(s) 239, 498, 732
Bme18I GGWCC 1 cut(s) 235
BmgBI CACGTC 1 cut(s) 309
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 2 cut(s) 237, 886
BmrFI CCNGG 3 cut(s) 239, 498, 732
BmsI GCATC 5 cut(s) 57, 204, 814, 864, 973
BmtI GCTAGC 1 cut(s) 543
BpiI GAAGAC 1 cut(s) 166
BpmI CTGGAG 1 cut(s) 714
Bpu1102I GCTNAGC 1 cut(s) 276
BpuEI CTTGAG 1 cut(s) 701
BpuMI CCSGG 2 cut(s) 239, 498
BsaHI GRCGYC 1 cut(s) 171
BsaJI CCNNGG 5 cut(s) 82, 157, 462, 497, 527
BsaWI WCCGGW 1 cut(s) 32
BsaXI ACNNNNNCTCC 4 cut(s) 149, 179, 458, 488
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse118I RCCGGY 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 791
BseBI CCWGG 1 cut(s) 732
BseDI CCNNGG 5 cut(s) 82, 157, 462, 497, 527
BseGI GGATG 1 cut(s) 484
BseLI CCNNNNNNNGG 1 cut(s) 223
BseNI ACTGG 1 cut(s) 791
BseRI GAGGAG 4 cut(s) 62, 65, 116, 479
BseXI GCAGC 3 cut(s) 32, 149, 433
BseYI CCCAGC 1 cut(s) 332
Bsh1236I CGCG 2 cut(s) 84, 297
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 4 cut(s) 284, 323, 350, 666
BshTI ACCGGT 1 cut(s) 32
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 2 cut(s) 306, 557
BsiSI CCGG 4 cut(s) 33, 168, 239, 497
BslFI GGGAC 1 cut(s) 514
BslI CCNNNNNNNGG 1 cut(s) 223
BsmFI GGGAC 1 cut(s) 514
BsnI GGCC 4 cut(s) 284, 323, 350, 666
Bsp1286I GDGCHC 2 cut(s) 306, 557
Bsp143I GATC 1 cut(s) 53
Bsp1720I GCTNAGC 1 cut(s) 276
BspACI CCGC 8 cut(s) 42, 67, 82, 84, 147, 150, 272, 437
BspANI GGCC 4 cut(s) 284, 323, 350, 666
BspFNI CGCG 2 cut(s) 84, 297
BspHI TCATGA 1 cut(s) 570
BspLI GGNNCC 2 cut(s) 237, 886
BspOI GCTAGC 1 cut(s) 543
BspPI GGATC 1 cut(s) 61
BsrFI RCCGGY 1 cut(s) 32
BsrI ACTGG 1 cut(s) 791
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 5 cut(s) 82, 157, 462, 497, 527
BssMI GATC 1 cut(s) 53
BssNI GRCGYC 1 cut(s) 171
Bst2UI CCWGG 1 cut(s) 732
Bst4CI ACNGT 5 cut(s) 94, 106, 411, 505, 836
BstACI GRCGYC 1 cut(s) 171
BstC8I GCNNGC 5 cut(s) 282, 286, 348, 398, 541
BstDEI CTNAG 1 cut(s) 276
BstDSI CCRYGG 2 cut(s) 82, 157
BstF5I GGATG 1 cut(s) 484
BstFNI CGCG 2 cut(s) 84, 297
BstHHI GCGC 1 cut(s) 297
BstKTI GATC 1 cut(s) 56
BstMBI GATC 1 cut(s) 53
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 5 cut(s) 168, 281, 294, 301, 443
BstNI CCWGG 1 cut(s) 732
BstSCI CCNGG 3 cut(s) 237, 496, 730
BstUI CGCG 2 cut(s) 84, 297
BstV1I GCAGC 3 cut(s) 32, 149, 433
BstV2I GAAGAC 1 cut(s) 166
BsuI GTATCC 1 cut(s) 426
BsuRI GGCC 4 cut(s) 284, 323, 350, 666
BtgI CCRYGG 2 cut(s) 82, 157
BtrI CACGTC 1 cut(s) 309
BtsCI GGATG 1 cut(s) 484
BtsIMutI CAGTG 1 cut(s) 510
Cac8I GCNNGC 5 cut(s) 282, 286, 348, 398, 541
CciI TCATGA 1 cut(s) 570
CfoI GCGC 1 cut(s) 297
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 1 cut(s) 235
Cfr42I CCGCGG 1 cut(s) 85
CseI GACGC 2 cut(s) 16, 160
Csp6I GTAC 1 cut(s) 391
CspAI ACCGGT 1 cut(s) 32
CviAII CATG 4 cut(s) 57, 212, 571, 842
CviQI GTAC 1 cut(s) 391
DdeI CTNAG 1 cut(s) 276
DpnI GATC 1 cut(s) 55
DpnII GATC 1 cut(s) 53
EaeI YGGCCR 2 cut(s) 321, 664
EciI GGCGGA 3 cut(s) 56, 136, 452
Eco147I AGGCCT 1 cut(s) 284
Eco47I GGWCC 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 730
FaeI CATG 4 cut(s) 60, 215, 574, 845
FaqI GGGAC 1 cut(s) 514
FatI CATG 4 cut(s) 56, 211, 570, 841
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 7 cut(s) 43, 46, 85, 150, 163, 273, 447
FokI GGATG 1 cut(s) 491
Fsp4HI GCNGC 7 cut(s) 43, 46, 85, 150, 163, 273, 447
FspBI CTAG 1 cut(s) 540
GlaI GCGC 1 cut(s) 296
GluI GCNGC 7 cut(s) 43, 46, 85, 150, 163, 273, 447
GsaI CCCAGC 1 cut(s) 336
GsuI CTGGAG 1 cut(s) 714
HaeIII GGCC 4 cut(s) 284, 323, 350, 666
HapII CCGG 4 cut(s) 33, 168, 239, 497
HgaI GACGC 2 cut(s) 16, 160
HhaI GCGC 1 cut(s) 297
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 4 cut(s) 60, 215, 574, 845
Hin6I GCGC 1 cut(s) 295
HinP1I GCGC 1 cut(s) 295
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HindIII AAGCTT 1 cut(s) 648
HinfI GANTC 2 cut(s) 137, 493
HpaII CCGG 4 cut(s) 33, 168, 239, 497
HphI GGTGA 7 cut(s) 22, 26, 253, 502, 524, 560, 793
Hpy166II GTNNAC 4 cut(s) 97, 157, 202, 784
Hpy188I TCNGA 1 cut(s) 53
Hpy188III TCNNGA 3 cut(s) 361, 571, 748
Hpy8I GTNNAC 4 cut(s) 97, 157, 202, 784
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 3 cut(s) 217, 463, 965
HpyCH4III ACNGT 5 cut(s) 94, 106, 411, 505, 836
HpyCH4IV ACGT 1 cut(s) 308
HpyCH4V TGCA 7 cut(s) 48, 195, 396, 586, 710, 877, 958
HpyF10VI GCNNNNNNNGC 5 cut(s) 168, 281, 294, 301, 443
HpyF3I CTNAG 1 cut(s) 276
HpySE526I ACGT 1 cut(s) 308
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 4 cut(s) 60, 215, 574, 845
HspAI GCGC 1 cut(s) 295
KspI CCGCGG 1 cut(s) 85
Kzo9I GATC 1 cut(s) 53
LmnI GCTCC 3 cut(s) 170, 226, 890
Lsp1109I GCAGC 3 cut(s) 32, 149, 433
LweI GCATC 5 cut(s) 57, 204, 814, 864, 973
MaeI CTAG 1 cut(s) 540
MaeII ACGT 1 cut(s) 308
MaeIII GTNAC 3 cut(s) 28, 830, 932
MalI GATC 1 cut(s) 55
MboI GATC 1 cut(s) 53
MboII GAAGA 3 cut(s) 166, 656, 696
MfeI CAATTG 1 cut(s) 899
MhlI GDGCHC 2 cut(s) 306, 557
MlsI TGGCCA 1 cut(s) 666
MluCI AATT 4 cut(s) 455, 595, 798, 899
MluNI TGGCCA 1 cut(s) 666
MmeI TCCRAC 3 cut(s) 213, 456, 619
Mox20I TGGCCA 1 cut(s) 666
MroXI GAANNNNTTC 1 cut(s) 578
MscI TGGCCA 1 cut(s) 666
MseI TTAA 1 cut(s) 246
Msp20I TGGCCA 1 cut(s) 666
MspA1I CMGCKG 1 cut(s) 84
MspI CCGG 4 cut(s) 33, 168, 239, 497
MspR9I CCNGG 3 cut(s) 239, 498, 732
MunI CAATTG 1 cut(s) 899
MvaI CCWGG 1 cut(s) 732
MvnI CGCG 2 cut(s) 84, 297
MwoI GCNNNNNNNGC 5 cut(s) 168, 281, 294, 301, 443
NciI CCSGG 2 cut(s) 239, 498
NdeII GATC 1 cut(s) 53
NheI GCTAGC 1 cut(s) 539
NlaIII CATG 4 cut(s) 60, 215, 574, 845
NlaIV GGNNCC 2 cut(s) 237, 886
NmeAIII GCCGAG 2 cut(s) 487, 552
NmuCI GTSAC 1 cut(s) 28
PagI TCATGA 1 cut(s) 570
PceI AGGCCT 1 cut(s) 284
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PdmI GAANNNNTTC 1 cut(s) 578
PfeI GAWTC 2 cut(s) 137, 493
PflFI GACNNNGTC 1 cut(s) 206
PinAI ACCGGT 1 cut(s) 32
PkrI GCNGC 7 cut(s) 44, 47, 86, 151, 164, 274, 448
Psp6I CCWGG 1 cut(s) 730
PspFI CCCAGC 1 cut(s) 332
PspGI CCWGG 1 cut(s) 730
PspN4I GGNNCC 2 cut(s) 237, 886
PspPI GGNCC 1 cut(s) 235
PsyI GACNNNGTC 1 cut(s) 206
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
SacII CCGCGG 1 cut(s) 85
SalI GTCGAC 1 cut(s) 200
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 7 cut(s) 43, 46, 85, 150, 163, 273, 447
Sau3AI GATC 1 cut(s) 53
Sau96I GGNCC 1 cut(s) 235
ScaI AGTACT 1 cut(s) 392
ScrFI CCNGG 3 cut(s) 239, 498, 732
SduI GDGCHC 2 cut(s) 306, 557
SfaNI GCATC 5 cut(s) 57, 204, 814, 864, 973
Sfr303I CCGCGG 1 cut(s) 85
SgrAI CRCCGGYG 1 cut(s) 32
SgrBI CCGCGG 1 cut(s) 85
SinI GGWCC 1 cut(s) 235
SmlI CTYRAG 1 cut(s) 680
SmoI CTYRAG 1 cut(s) 680
Sse9I AATT 4 cut(s) 455, 595, 798, 899
SseBI AGGCCT 1 cut(s) 284
SsiI CCGC 8 cut(s) 42, 67, 82, 84, 147, 150, 272, 437
SspMI CTAG 1 cut(s) 540
StuI AGGCCT 1 cut(s) 284
StyD4I CCNGG 3 cut(s) 237, 496, 730
TaaI ACNGT 5 cut(s) 94, 106, 411, 505, 836
TaiI ACGT 1 cut(s) 311
TaqI TCGA 6 cut(s) 5, 78, 120, 129, 201, 425
TasI AATT 4 cut(s) 455, 595, 798, 899
TatI WGTACW 1 cut(s) 390
TauI GCSGC 4 cut(s) 45, 87, 152, 275
TfiI GAWTC 2 cut(s) 137, 493
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TscAI CASTG 1 cut(s) 510
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 3 cut(s) 45, 162, 446
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 5 cut(s) 587, 590, 830, 882, 926
TspRI CASTG 1 cut(s) 510
Tth111I GACNNNGTC 1 cut(s) 206
VpaK11BI GGWCC 1 cut(s) 235
XapI RAATTY 2 cut(s) 455, 798
XmiI GTMKAC 1 cut(s) 201
XmnI GAANNNNTTC 1 cut(s) 578
XspI CTAG 1 cut(s) 540
ZrmI AGTACT 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.