FvH4_3g39460

cinnamoyl-CoA reductase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003662
Physical Location & Seq
Reverse (-)
33572323 .. 33575647
3325 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g39460.t1

Sequence Viewer

Length: 975 bp
ATGTCGAAGCAGGGTGCTGAGGTCGTATGCGTCACCGGTGGAAGCGGCTGCATTGGATCATGGCTCGTCCGCCTCCTCCTCGACCGCGGCTACTCCGTCCACGCCACCGTCAAAAACCTCCATGACGAGGCCGAGACGAAGCATCTAGAAGCCCTAGAAGGAGCCTCCACCCGTCTCCGTCTCTTCCAGATCGACCTCCTCGACCGCGCTTCCATCTCCGCCGCCGTCCACGGCTGCTCCGGCGTCTTCCACCTCGCCTCTCCTTGCATCGTTGACCAAGTCCATGACCCCGAGAAGGAGCTTCTGGATCCGGCGATTAAAGGAACTCTGAATGTTCTGACGGCGGCGGAGGAGGCTGGAGTCAGGCGCGTGGTGGTCACGTCCTCCGTCTCGGCCATCCATCCCAGCCCTAGCTGGCCTGCTGATGTGGTCAAGAGAGAGGATTGCTGGACTGATGTTGAGTACTGCAAGAAAAAGGGATTATGGTATCCTTTGTCGAAAACGCTGGCGGAGAAAGCGGCTTGGGATTTTGCCAAGGAGAAGGGGCTGGATGTGGTTGTGGTGAATCCGGGGACGGTGATGGGTGATGTTATTTCGCCCAGGCTCAATGCTAGCATGTTAATGCTTGTGCGCCTTCTCGAAGGCTGCACTGAAACATATGAGGATTTCTTTATGGGTTCTGTACATTTTAAAGATGTAGCTCTAGCACACATTTTAGTTTATGAGAACAAATCAGCCACTGGTAGGCACTCGTGCGTTGAGGCGATATCACATTATGGTGATTTTGTGGCAAAGGTAGCTGAACTTTACCCTGAGTACGAGGTTCCCAGTTTGCCAAAGGATACTCAGCCTGGTTTGTTGAGGGAAAAGACTGGAGCGAAAAAGCTAATGGACTTGGGTATAGACTTCATCCCCATGGATCAAATTATCAAGGATTCCATTGAGAGCCTAAAGAGCAAGGGATTTATTTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

325

Amino Acids

35.44

Weight (kDa)

5.68

Isoelectric Point (pI)

28.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 8 - 140 3.4e-12 NmrA-like family
Epimerase PF01370 9 - 245 1.7e-26 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 9 - 136 6e-10 Polysaccharide biosynthesis protein
KR PF08659 9 - 134 3.3e-08 KR domain
3Beta_HSD PF01073 11 - 243 7.2e-20 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 129 1.4e-17 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 209 1.6e-09 Male sterility protein
NAD_binding_10 PF13460 13 - 193 1.6e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 87, 207, 369
AclWI GGATC 4 cut(s) 64, 302, 315, 927
AcoI YGGCCR 1 cut(s) 393
AcyI GRCGYC 1 cut(s) 243
AfaI GTAC 3 cut(s) 464, 684, 818
AfiI CCNNNNNNNGG 3 cut(s) 127, 295, 744
AgeI ACCGGT 1 cut(s) 35
AjiI CACGTC 1 cut(s) 381
AjnI CCWGG 2 cut(s) 599, 850
AluBI AGCT 5 cut(s) 301, 414, 701, 800, 886
AluI AGCT 5 cut(s) 301, 414, 701, 800, 886
Alw26I GTCTC 4 cut(s) 128, 179, 185, 394
AlwI GGATC 4 cut(s) 64, 302, 315, 927
AlwNI CAGNNNCTG 1 cut(s) 740
Ama87I CYCGRG 1 cut(s) 290
AoxI GGCC 3 cut(s) 129, 393, 416
ApeKI GCWGC 3 cut(s) 48, 234, 645
AsiGI ACCGGT 1 cut(s) 35
AspLEI GCGC 3 cut(s) 209, 369, 633
AsuC2I CCSGG 1 cut(s) 570
AsuHPI GGTGA 5 cut(s) 25, 574, 589, 596, 791
AsuNHI GCTAGC 1 cut(s) 611
AvaI CYCGRG 1 cut(s) 290
BamHI GGATCC 1 cut(s) 307
BauI CACGAG 1 cut(s) 751
BbsI GAAGAC 1 cut(s) 238
BbvCI CCTCAGC 1 cut(s) 18
BbvI GCAGC 3 cut(s) 35, 221, 632
BccI CCATC 4 cut(s) 221, 404, 408, 574
BceAI ACGGC 3 cut(s) 209, 247, 357
BciT130I CCWGG 2 cut(s) 601, 852
BciVI GTATCC 2 cut(s) 498, 835
BcnI CCSGG 1 cut(s) 570
BcoDI GTCTC 4 cut(s) 128, 179, 185, 394
BfaI CTAG 5 cut(s) 146, 155, 411, 612, 704
BfuI GTATCC 2 cut(s) 498, 835
BisI GCNGC 8 cut(s) 46, 49, 88, 222, 235, 345, 519, 646
BlsI GCNGC 8 cut(s) 47, 50, 89, 223, 236, 346, 520, 647
BmcAI AGTACT 1 cut(s) 464
Bme1390I CCNGG 3 cut(s) 570, 601, 852
BmeT110I CYCGRG 1 cut(s) 290
BmgBI CACGTC 1 cut(s) 381
BmiI GGNNCC 3 cut(s) 163, 309, 825
BmrFI CCNGG 3 cut(s) 570, 601, 852
BmrI ACTGGG 1 cut(s) 822
BmsI GCATC 2 cut(s) 151, 276
BmtI GCTAGC 1 cut(s) 615
BmuI ACTGGG 1 cut(s) 822
BpiI GAAGAC 1 cut(s) 238
BpmI CTGGAG 2 cut(s) 378, 894
Bpu10I CCTNAGC 1 cut(s) 18
BpuMI CCSGG 1 cut(s) 570
BsaHI GRCGYC 1 cut(s) 243
BsaJI CCNNGG 6 cut(s) 85, 229, 534, 569, 599, 915
BsaWI WCCGGW 1 cut(s) 35
BsaXI ACNNNNNCTCC 2 cut(s) 221, 251
Bsc4I CCNNNNNNNGG 3 cut(s) 127, 295, 744
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 3 cut(s) 745, 828, 877
BseBI CCWGG 2 cut(s) 601, 852
BseDI CCNNGG 6 cut(s) 85, 229, 534, 569, 599, 915
BseGI GGATG 4 cut(s) 396, 400, 556, 909
BseLI CCNNNNNNNGG 3 cut(s) 127, 295, 744
BseMII CTCAG 3 cut(s) 9, 804, 860
BseNI ACTGG 3 cut(s) 745, 828, 877
BseRI GAGGAG 4 cut(s) 65, 68, 188, 365
BseXI GCAGC 3 cut(s) 35, 221, 632
BseYI CCCAGC 1 cut(s) 404
BsgI GTGCAG 1 cut(s) 631
Bsh1236I CGCG 3 cut(s) 87, 207, 369
Bsh1285I CGRYCG 2 cut(s) 85, 205
BshFI GGCC 3 cut(s) 131, 395, 418
BshTI ACCGGT 1 cut(s) 35
BsiEI CGRYCG 2 cut(s) 85, 205
BsiHKCI CYCGRG 1 cut(s) 290
BsiSI CCGG 4 cut(s) 36, 240, 311, 569
BslFI GGGAC 1 cut(s) 586
BslI CCNNNNNNNGG 3 cut(s) 127, 295, 744
BsmAI GTCTC 4 cut(s) 128, 179, 185, 394
BsmBI CGTCTC 4 cut(s) 128, 179, 185, 394
BsmFI GGGAC 1 cut(s) 586
BsnI GGCC 3 cut(s) 131, 395, 418
BsoBI CYCGRG 1 cut(s) 290
Bsp1407I TGTACA 1 cut(s) 682
Bsp143I GATC 4 cut(s) 56, 189, 307, 919
Bsp19I CCATGG 1 cut(s) 915
BspANI GGCC 3 cut(s) 131, 395, 418
BspCNI CTCAG 3 cut(s) 10, 805, 859
BspFNI CGCG 3 cut(s) 87, 207, 369
BspLI GGNNCC 3 cut(s) 163, 309, 825
BspOI GCTAGC 1 cut(s) 615
BspPI GGATC 4 cut(s) 64, 302, 315, 927
BsrFI RCCGGY 1 cut(s) 35
BsrGI TGTACA 1 cut(s) 682
BsrI ACTGG 3 cut(s) 745, 828, 877
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 6 cut(s) 85, 229, 534, 569, 599, 915
BssMI GATC 4 cut(s) 56, 189, 307, 919
BssNI GRCGYC 1 cut(s) 243
BssSI CACGAG 1 cut(s) 751
BssT1I CCWWGG 2 cut(s) 534, 915
Bst2BI CACGAG 1 cut(s) 751
Bst2UI CCWGG 2 cut(s) 601, 852
Bst4CI ACNGT 2 cut(s) 109, 577
Bst6I CTCTTC 1 cut(s) 188
BstACI GRCGYC 1 cut(s) 243
BstAUI TGTACA 1 cut(s) 682
BstC8I GCNNGC 4 cut(s) 416, 420, 507, 613
BstDEI CTNAG 3 cut(s) 18, 813, 846
BstDSI CCRYGG 3 cut(s) 85, 229, 915
BstF5I GGATG 4 cut(s) 396, 400, 556, 909
BstFNI CGCG 3 cut(s) 87, 207, 369
BstHHI GCGC 3 cut(s) 209, 369, 633
BstKTI GATC 4 cut(s) 59, 192, 310, 922
BstMAI GTCTC 4 cut(s) 128, 179, 185, 394
BstMBI GATC 4 cut(s) 56, 189, 307, 919
BstMCI CGRYCG 2 cut(s) 85, 205
BstMWI GCNNNNNNNGC 5 cut(s) 240, 353, 515, 797, 954
BstNI CCWGG 2 cut(s) 601, 852
BstNSI RCATGY 1 cut(s) 619
BstSCI CCNGG 3 cut(s) 568, 599, 850
BstUI CGCG 3 cut(s) 87, 207, 369
BstV1I GCAGC 3 cut(s) 35, 221, 632
BstV2I GAAGAC 1 cut(s) 238
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BsuI GTATCC 2 cut(s) 498, 835
BsuRI GGCC 3 cut(s) 131, 395, 418
BtgI CCRYGG 3 cut(s) 85, 229, 915
BtrI CACGTC 1 cut(s) 381
BtsCI GGATG 4 cut(s) 396, 400, 556, 909
BtsIMutI CAGTG 2 cut(s) 648, 738
Cac8I GCNNGC 4 cut(s) 416, 420, 507, 613
CaiI CAGNNNCTG 1 cut(s) 740
CfoI GCGC 3 cut(s) 209, 369, 633
Cfr10I RCCGGY 1 cut(s) 35
Cfr42I CCGCGG 1 cut(s) 88
CseI GACGC 2 cut(s) 19, 232
Csp6I GTAC 3 cut(s) 463, 683, 817
CspAI ACCGGT 1 cut(s) 35
CviAII CATG 5 cut(s) 60, 122, 284, 616, 916
CviQI GTAC 3 cut(s) 463, 683, 817
DdeI CTNAG 3 cut(s) 18, 813, 846
DpnI GATC 4 cut(s) 58, 191, 309, 921
DpnII GATC 4 cut(s) 56, 189, 307, 919
DraI TTTAAA 1 cut(s) 691
EaeI YGGCCR 1 cut(s) 393
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
EciI GGCGGA 4 cut(s) 59, 208, 362, 524
Eco130I CCWWGG 2 cut(s) 534, 915
Eco32I GATATC 1 cut(s) 768
Eco88I CYCGRG 1 cut(s) 290
EcoRII CCWGG 2 cut(s) 599, 850
EcoRV GATATC 1 cut(s) 768
EcoT14I CCWWGG 2 cut(s) 534, 915
ErhI CCWWGG 2 cut(s) 534, 915
Esp3I CGTCTC 4 cut(s) 128, 179, 185, 394
FaeI CATG 5 cut(s) 63, 125, 287, 619, 919
FaqI GGGAC 1 cut(s) 586
FatI CATG 5 cut(s) 59, 121, 283, 615, 915
FauNDI CATATG 1 cut(s) 658
Fnu4HI GCNGC 8 cut(s) 46, 49, 88, 222, 235, 345, 519, 646
FokI GGATG 4 cut(s) 383, 387, 563, 896
Fsp4HI GCNGC 8 cut(s) 46, 49, 88, 222, 235, 345, 519, 646
FspBI CTAG 5 cut(s) 146, 155, 411, 612, 704
GlaI GCGC 3 cut(s) 208, 368, 632
GluI GCNGC 8 cut(s) 46, 49, 88, 222, 235, 345, 519, 646
GsaI CCCAGC 1 cut(s) 408
GsuI CTGGAG 2 cut(s) 378, 894
HaeIII GGCC 3 cut(s) 131, 395, 418
HapII CCGG 4 cut(s) 36, 240, 311, 569
HgaI GACGC 2 cut(s) 19, 232
HhaI GCGC 3 cut(s) 209, 369, 633
Hin1I GRCGYC 1 cut(s) 243
Hin1II CATG 5 cut(s) 63, 125, 287, 619, 919
Hin6I GCGC 3 cut(s) 207, 367, 631
HinP1I GCGC 3 cut(s) 207, 367, 631
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 3 cut(s) 360, 565, 935
HpaII CCGG 4 cut(s) 36, 240, 311, 569
HphI GGTGA 5 cut(s) 25, 574, 589, 596, 791
Hpy166II GTNNAC 3 cut(s) 100, 229, 274
Hpy188I TCNGA 2 cut(s) 330, 339
Hpy188III TCNNGA 5 cut(s) 146, 187, 305, 433, 638
Hpy8I GTNNAC 3 cut(s) 100, 229, 274
HpyAV CCTTC 5 cut(s) 152, 289, 535, 635, 644
HpyCH4III ACNGT 2 cut(s) 109, 577
HpyCH4IV ACGT 1 cut(s) 380
HpyCH4V TGCA 4 cut(s) 51, 267, 468, 648
HpyF10VI GCNNNNNNNGC 5 cut(s) 240, 353, 515, 797, 954
HpyF3I CTNAG 3 cut(s) 18, 813, 846
HpySE526I ACGT 1 cut(s) 380
Hsp92I GRCGYC 1 cut(s) 243
Hsp92II CATG 5 cut(s) 63, 125, 287, 619, 919
HspAI GCGC 3 cut(s) 207, 367, 631
KspI CCGCGG 1 cut(s) 88
Kzo9I GATC 4 cut(s) 56, 189, 307, 919
LmnI GCTCC 4 cut(s) 161, 242, 298, 875
Lsp1109I GCAGC 3 cut(s) 35, 221, 632
LweI GCATC 2 cut(s) 151, 276
MaeI CTAG 5 cut(s) 146, 155, 411, 612, 704
MaeII ACGT 1 cut(s) 380
MaeIII GTNAC 2 cut(s) 31, 376
MalI GATC 4 cut(s) 58, 191, 309, 921
MboI GATC 4 cut(s) 56, 189, 307, 919
MboII GAAGA 2 cut(s) 175, 238
MflI RGATCY 1 cut(s) 307
MluCI AATT 1 cut(s) 924
MlyI GAGTC 1 cut(s) 369
MseI TTAA 3 cut(s) 318, 620, 690
MslI CAYNNNNRTG 3 cut(s) 620, 777, 914
MspA1I CMGCKG 1 cut(s) 87
MspI CCGG 4 cut(s) 36, 240, 311, 569
MspR9I CCNGG 3 cut(s) 570, 601, 852
MvaI CCWGG 2 cut(s) 601, 852
MvnI CGCG 3 cut(s) 87, 207, 369
MwoI GCNNNNNNNGC 5 cut(s) 240, 353, 515, 797, 954
NciI CCSGG 1 cut(s) 570
NcoI CCATGG 1 cut(s) 915
NdeI CATATG 1 cut(s) 658
NdeII GATC 4 cut(s) 56, 189, 307, 919
NheI GCTAGC 1 cut(s) 611
NlaIII CATG 5 cut(s) 63, 125, 287, 619, 919
NlaIV GGNNCC 3 cut(s) 163, 309, 825
NmeAIII GCCGAG 2 cut(s) 157, 371
NmuCI GTSAC 2 cut(s) 31, 376
NspI RCATGY 1 cut(s) 619
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PfeI GAWTC 2 cut(s) 565, 935
PflFI GACNNNGTC 1 cut(s) 278
PinAI ACCGGT 1 cut(s) 35
PkrI GCNGC 8 cut(s) 47, 50, 89, 223, 236, 346, 520, 647
PleI GAGTC 1 cut(s) 368
PpsI GAGTC 1 cut(s) 368
Psp6I CCWGG 2 cut(s) 599, 850
PspFI CCCAGC 1 cut(s) 404
PspGI CCWGG 2 cut(s) 599, 850
PspN4I GGNNCC 3 cut(s) 163, 309, 825
PstNI CAGNNNCTG 1 cut(s) 740
PsuI RGATCY 1 cut(s) 307
PsyI GACNNNGTC 1 cut(s) 278
RsaI GTAC 3 cut(s) 464, 684, 818
RsaNI GTAC 3 cut(s) 463, 683, 817
RseI CAYNNNNRTG 3 cut(s) 620, 777, 914
SacII CCGCGG 1 cut(s) 88
SaqAI TTAA 3 cut(s) 318, 620, 690
SatI GCNGC 8 cut(s) 46, 49, 88, 222, 235, 345, 519, 646
Sau3AI GATC 4 cut(s) 56, 189, 307, 919
ScaI AGTACT 1 cut(s) 464
SchI GAGTC 1 cut(s) 369
ScrFI CCNGG 3 cut(s) 570, 601, 852
SfaNI GCATC 2 cut(s) 151, 276
Sfr303I CCGCGG 1 cut(s) 88
SgrAI CRCCGGYG 1 cut(s) 35
SgrBI CCGCGG 1 cut(s) 88
SmiMI CAYNNNNRTG 3 cut(s) 620, 777, 914
Sse9I AATT 1 cut(s) 924
SspMI CTAG 5 cut(s) 146, 155, 411, 612, 704
StyD4I CCNGG 3 cut(s) 568, 599, 850
StyI CCWWGG 2 cut(s) 534, 915
TaaI ACNGT 2 cut(s) 109, 577
TaiI ACGT 1 cut(s) 383
TaqI TCGA 6 cut(s) 5, 81, 192, 201, 497, 639
TasI AATT 1 cut(s) 924
TatI WGTACW 2 cut(s) 462, 682
TauI GCSGC 5 cut(s) 48, 90, 224, 347, 521
TfiI GAWTC 2 cut(s) 565, 935
Tru1I TTAA 3 cut(s) 318, 620, 690
Tru9I TTAA 3 cut(s) 318, 620, 690
TscAI CASTG 2 cut(s) 655, 745
TseFI GTSAC 2 cut(s) 31, 376
TseI GCWGC 3 cut(s) 48, 234, 645
Tsp45I GTSAC 2 cut(s) 31, 376
TspDTI ATGAA 1 cut(s) 898
TspGWI ACGGA 3 cut(s) 85, 167, 376
TspRI CASTG 2 cut(s) 655, 745
Tth111I GACNNNGTC 1 cut(s) 278
XbaI TCTAGA 1 cut(s) 145
XceI RCATGY 1 cut(s) 619
XspI CTAG 5 cut(s) 146, 155, 411, 612, 704
ZrmI AGTACT 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.