Rh3DG309400

cinnamoyl-CoA reductase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0003662
Physical Location & Seq
Forward (+)
33211066 .. 33212200
1135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG309400.1

Sequence Viewer

Length: 672 bp
ATGTCGAAGCAGGGTGAGGTCGTATGCGTCACCGGTGGAAGCGGCTGCATCGGATCATGGGTTGTCCGCCTCCTCCTCGACCGCGGCTACACCGTCCACGCCACCGTCAAAGACCTTATCGACCTCCTCGACTACGATTCCATCTCCGCCGCAGTCCACGGCTGCTCCGGCGTTTTCCACCTCGCCTCTCCTTGCATCGTCGACCAAGTCCATGACCCCCAGAAGGAGCTGTTGGACCCGGTGATTAAAGGAACGCTGAATGTTCTAACGGCGGCTAAGCAGGCCTGCGTGAAGCGCGTGGTGCTCACGTCCTCCATATCGGCCATCACTCCCAGCCCTACTTGGCAGGCCGATGTCGTCAAGAGAGAGGATTGCTGGACTGATGTTGAGTACTGCAAGCAGAAAGGACTGTGGTATCCTTTATCGAAAACTCTGGCGGAGAAAGCTGCGTGGGAATTTGCCGAGGAGAAGGGGTTGGATGTGGTTGTGGTGAATCCGGGAACAGTGATGGGTGATGTTATATCGCCGAGGCTCAATGCTAGCAAAGGTCAGAGGAAGAAGAGGGTCGCTAGCTACAAGGTGTACACTCTGGAGGGGAAGCTGAAAGGGACTCTCAGGAAGAGCTTCAGGTGGCTCAAGCAGACCTGTGATAGAGTGGTCTATGACCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

223

Amino Acids

24.61

Weight (kDa)

8.95

Isoelectric Point (pI)

29.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 8 - 173 2.9e-21 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 12 - 170 2.8e-07 NAD(P)H-binding
3Beta_HSD PF01073 40 - 150 3.1e-12 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 52 - 187 1.2e-08 Male sterility protein
DUF3511 PF12023 184 - 221 1.1e-19 Domain of unknown function (DUF3511)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 201
AccII CGCG 2 cut(s) 84, 297
AciI CCGC 8 cut(s) 42, 67, 82, 84, 147, 150, 272, 437
AclWI GGATC 1 cut(s) 61
AcoI YGGCCR 1 cut(s) 321
AcsI RAATTY 1 cut(s) 455
AcuI CTGAAG 1 cut(s) 610
AfaI GTAC 2 cut(s) 392, 584
AfiI CCNNNNNNNGG 1 cut(s) 223
AgeI ACCGGT 2 cut(s) 32, 666
AjiI CACGTC 1 cut(s) 309
AjuI GAANNNNNNNTTGG 2 cut(s) 215, 247
AluBI AGCT 5 cut(s) 229, 446, 573, 601, 624
AluI AGCT 5 cut(s) 229, 446, 573, 601, 624
Alw21I GWGCWC 1 cut(s) 306
AlwI GGATC 1 cut(s) 61
AoxI GGCC 3 cut(s) 282, 321, 348
ApeKI GCWGC 3 cut(s) 45, 162, 446
ApoI RAATTY 1 cut(s) 455
AsiGI ACCGGT 2 cut(s) 32, 666
Asp700I GAANNNNTTC 1 cut(s) 623
AspLEI GCGC 1 cut(s) 297
AspS9I GGNCC 1 cut(s) 235
AsuC2I CCSGG 2 cut(s) 239, 498
AsuHPI GGTGA 5 cut(s) 22, 26, 253, 502, 524
AsuNHI GCTAGC 2 cut(s) 539, 569
AvaII GGWCC 1 cut(s) 235
BaeI ACNNNNGTAYC 2 cut(s) 398, 431
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 3 cut(s) 32, 149, 433
BccI CCATC 3 cut(s) 149, 332, 502
BceAI ACGGC 2 cut(s) 175, 285
BciVI GTATCC 1 cut(s) 426
BcnI CCSGG 2 cut(s) 239, 498
BfaI CTAG 2 cut(s) 540, 570
BfuI GTATCC 1 cut(s) 426
BisI GCNGC 7 cut(s) 43, 46, 85, 150, 163, 273, 447
BlpI GCTNAGC 1 cut(s) 276
BlsI GCNGC 7 cut(s) 44, 47, 86, 151, 164, 274, 448
BmcAI AGTACT 1 cut(s) 392
Bme1390I CCNGG 2 cut(s) 239, 498
Bme18I GGWCC 1 cut(s) 235
BmgBI CACGTC 1 cut(s) 309
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 1 cut(s) 237
BmrFI CCNGG 2 cut(s) 239, 498
BmsI GCATC 2 cut(s) 57, 204
BmtI GCTAGC 2 cut(s) 543, 573
BpmI CTGGAG 1 cut(s) 611
Bpu1102I GCTNAGC 1 cut(s) 276
BpuEI CTTGAG 1 cut(s) 620
BpuMI CCSGG 2 cut(s) 239, 498
BsaJI CCNNGG 4 cut(s) 82, 157, 462, 527
BsaWI WCCGGW 2 cut(s) 32, 666
BsaXI ACNNNNNCTCC 4 cut(s) 149, 179, 458, 488
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse118I RCCGGY 2 cut(s) 32, 666
BseDI CCNNGG 4 cut(s) 82, 157, 462, 527
BseGI GGATG 1 cut(s) 484
BseLI CCNNNNNNNGG 1 cut(s) 223
BseMII CTCAG 1 cut(s) 628
BseRI GAGGAG 4 cut(s) 62, 65, 116, 479
BseXI GCAGC 3 cut(s) 32, 149, 433
BseYI CCCAGC 1 cut(s) 332
Bsh1236I CGCG 2 cut(s) 84, 297
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 3 cut(s) 284, 323, 350
BshTI ACCGGT 2 cut(s) 32, 666
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 306
BsiSI CCGG 5 cut(s) 33, 168, 239, 497, 667
BslFI GGGAC 1 cut(s) 622
BslI CCNNNNNNNGG 1 cut(s) 223
BsmFI GGGAC 1 cut(s) 622
BsnI GGCC 3 cut(s) 284, 323, 350
Bsp1286I GDGCHC 1 cut(s) 306
Bsp1407I TGTACA 1 cut(s) 582
Bsp143I GATC 1 cut(s) 53
Bsp1720I GCTNAGC 1 cut(s) 276
BspACI CCGC 8 cut(s) 42, 67, 82, 84, 147, 150, 272, 437
BspANI GGCC 3 cut(s) 284, 323, 350
BspCNI CTCAG 1 cut(s) 627
BspFNI CGCG 2 cut(s) 84, 297
BspLI GGNNCC 1 cut(s) 237
BspOI GCTAGC 2 cut(s) 543, 573
BspPI GGATC 1 cut(s) 61
BspQI GCTCTTC 1 cut(s) 614
BsrFI RCCGGY 2 cut(s) 32, 666
BsrGI TGTACA 1 cut(s) 582
BssAI RCCGGY 2 cut(s) 32, 666
BssECI CCNNGG 4 cut(s) 82, 157, 462, 527
BssMI GATC 1 cut(s) 53
Bst4CI ACNGT 4 cut(s) 94, 106, 411, 505
Bst6I CTCTTC 2 cut(s) 554, 614
BstAUI TGTACA 1 cut(s) 582
BstC8I GCNNGC 6 cut(s) 282, 286, 348, 398, 541, 571
BstDEI CTNAG 2 cut(s) 276, 614
BstDSI CCRYGG 2 cut(s) 82, 157
BstF5I GGATG 1 cut(s) 484
BstFNI CGCG 2 cut(s) 84, 297
BstHHI GCGC 1 cut(s) 297
BstKTI GATC 1 cut(s) 56
BstMBI GATC 1 cut(s) 53
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 5 cut(s) 168, 281, 294, 301, 443
BstSCI CCNGG 2 cut(s) 237, 496
BstUI CGCG 2 cut(s) 84, 297
BstV1I GCAGC 3 cut(s) 32, 149, 433
BsuI GTATCC 1 cut(s) 426
BsuRI GGCC 3 cut(s) 284, 323, 350
BtgI CCRYGG 2 cut(s) 82, 157
BtrI CACGTC 1 cut(s) 309
BtsCI GGATG 1 cut(s) 484
BtsIMutI CAGTG 1 cut(s) 510
Cac8I GCNNGC 6 cut(s) 282, 286, 348, 398, 541, 571
CfoI GCGC 1 cut(s) 297
Cfr10I RCCGGY 2 cut(s) 32, 666
Cfr13I GGNCC 1 cut(s) 235
Cfr42I CCGCGG 1 cut(s) 85
CseI GACGC 1 cut(s) 16
Csp6I GTAC 2 cut(s) 391, 583
CspAI ACCGGT 2 cut(s) 32, 666
CviAII CATG 2 cut(s) 57, 212
CviQI GTAC 2 cut(s) 391, 583
DdeI CTNAG 2 cut(s) 276, 614
DpnI GATC 1 cut(s) 55
DpnII GATC 1 cut(s) 53
EaeI YGGCCR 1 cut(s) 321
Eam1104I CTCTTC 2 cut(s) 554, 614
EarI CTCTTC 2 cut(s) 554, 614
EciI GGCGGA 3 cut(s) 56, 136, 452
Eco147I AGGCCT 1 cut(s) 284
Eco47I GGWCC 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 610
FaeI CATG 2 cut(s) 60, 215
FaiI YATR 6 cut(s) 25, 58, 213, 317, 521, 663
FaqI GGGAC 1 cut(s) 622
FatI CATG 2 cut(s) 56, 211
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 7 cut(s) 43, 46, 85, 150, 163, 273, 447
FokI GGATG 1 cut(s) 491
Fsp4HI GCNGC 7 cut(s) 43, 46, 85, 150, 163, 273, 447
FspBI CTAG 2 cut(s) 540, 570
GlaI GCGC 1 cut(s) 296
GluI GCNGC 7 cut(s) 43, 46, 85, 150, 163, 273, 447
GsaI CCCAGC 1 cut(s) 336
GsuI CTGGAG 1 cut(s) 611
HaeIII GGCC 3 cut(s) 284, 323, 350
HapII CCGG 5 cut(s) 33, 168, 239, 497, 667
HgaI GACGC 1 cut(s) 16
HhaI GCGC 1 cut(s) 297
Hin1II CATG 2 cut(s) 60, 215
Hin6I GCGC 1 cut(s) 295
HinP1I GCGC 1 cut(s) 295
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 3 cut(s) 137, 493, 610
HpaII CCGG 5 cut(s) 33, 168, 239, 497, 667
HphI GGTGA 5 cut(s) 22, 26, 253, 502, 524
Hpy166II GTNNAC 5 cut(s) 97, 157, 202, 583, 585
Hpy188I TCNGA 2 cut(s) 53, 552
Hpy188III TCNNGA 3 cut(s) 361, 590, 616
Hpy8I GTNNAC 5 cut(s) 97, 157, 202, 583, 585
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 2 cut(s) 217, 463
HpyCH4III ACNGT 4 cut(s) 94, 106, 411, 505
HpyCH4IV ACGT 1 cut(s) 308
HpyCH4V TGCA 3 cut(s) 48, 195, 396
HpyF10VI GCNNNNNNNGC 5 cut(s) 168, 281, 294, 301, 443
HpyF3I CTNAG 2 cut(s) 276, 614
HpySE526I ACGT 1 cut(s) 308
Hsp92II CATG 2 cut(s) 60, 215
HspAI GCGC 1 cut(s) 295
KspI CCGCGG 1 cut(s) 85
Kzo9I GATC 1 cut(s) 53
LguI GCTCTTC 1 cut(s) 614
LmnI GCTCC 2 cut(s) 170, 226
Lsp1109I GCAGC 3 cut(s) 32, 149, 433
LweI GCATC 2 cut(s) 57, 204
MaeI CTAG 2 cut(s) 540, 570
MaeII ACGT 1 cut(s) 308
MaeIII GTNAC 1 cut(s) 28
MalI GATC 1 cut(s) 55
MboI GATC 1 cut(s) 53
MboII GAAGA 3 cut(s) 568, 571, 631
MhlI GDGCHC 1 cut(s) 306
MluCI AATT 1 cut(s) 455
MlyI GAGTC 1 cut(s) 604
MmeI TCCRAC 2 cut(s) 213, 456
MroXI GAANNNNTTC 1 cut(s) 623
MseI TTAA 1 cut(s) 246
MspA1I CMGCKG 1 cut(s) 84
MspI CCGG 5 cut(s) 33, 168, 239, 497, 667
MspR9I CCNGG 2 cut(s) 239, 498
MvnI CGCG 2 cut(s) 84, 297
MwoI GCNNNNNNNGC 5 cut(s) 168, 281, 294, 301, 443
NciI CCSGG 2 cut(s) 239, 498
NdeII GATC 1 cut(s) 53
NheI GCTAGC 2 cut(s) 539, 569
NlaIII CATG 2 cut(s) 60, 215
NlaIV GGNNCC 1 cut(s) 237
NmeAIII GCCGAG 2 cut(s) 487, 552
NmuCI GTSAC 1 cut(s) 28
PceI AGGCCT 1 cut(s) 284
PciSI GCTCTTC 1 cut(s) 614
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PdmI GAANNNNTTC 1 cut(s) 623
PfeI GAWTC 2 cut(s) 137, 493
PflFI GACNNNGTC 1 cut(s) 206
PfoI TCCNGGA 1 cut(s) 496
PinAI ACCGGT 2 cut(s) 32, 666
PkrI GCNGC 7 cut(s) 44, 47, 86, 151, 164, 274, 448
PleI GAGTC 1 cut(s) 604
PpsI GAGTC 1 cut(s) 604
PspFI CCCAGC 1 cut(s) 332
PspN4I GGNNCC 1 cut(s) 237
PspPI GGNCC 1 cut(s) 235
PsyI GACNNNGTC 1 cut(s) 206
RsaI GTAC 2 cut(s) 392, 584
RsaNI GTAC 2 cut(s) 391, 583
SacII CCGCGG 1 cut(s) 85
SalI GTCGAC 1 cut(s) 200
SapI GCTCTTC 1 cut(s) 614
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 7 cut(s) 43, 46, 85, 150, 163, 273, 447
Sau3AI GATC 1 cut(s) 53
Sau96I GGNCC 1 cut(s) 235
ScaI AGTACT 1 cut(s) 392
SchI GAGTC 1 cut(s) 604
ScrFI CCNGG 2 cut(s) 239, 498
SduI GDGCHC 1 cut(s) 306
SfaNI GCATC 2 cut(s) 57, 204
Sfr303I CCGCGG 1 cut(s) 85
SgrAI CRCCGGYG 1 cut(s) 32
SgrBI CCGCGG 1 cut(s) 85
SinI GGWCC 1 cut(s) 235
SmlI CTYRAG 1 cut(s) 635
SmoI CTYRAG 1 cut(s) 635
Sse9I AATT 1 cut(s) 455
SseBI AGGCCT 1 cut(s) 284
SsiI CCGC 8 cut(s) 42, 67, 82, 84, 147, 150, 272, 437
SspMI CTAG 2 cut(s) 540, 570
StuI AGGCCT 1 cut(s) 284
StyD4I CCNGG 2 cut(s) 237, 496
TaaI ACNGT 4 cut(s) 94, 106, 411, 505
TaiI ACGT 1 cut(s) 311
TaqI TCGA 6 cut(s) 5, 78, 120, 129, 201, 425
TasI AATT 1 cut(s) 455
TatI WGTACW 2 cut(s) 390, 582
TauI GCSGC 4 cut(s) 45, 87, 152, 275
TfiI GAWTC 2 cut(s) 137, 493
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TscAI CASTG 1 cut(s) 510
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 3 cut(s) 45, 162, 446
Tsp45I GTSAC 1 cut(s) 28
TspRI CASTG 1 cut(s) 510
Tth111I GACNNNGTC 1 cut(s) 206
VpaK11BI GGWCC 1 cut(s) 235
XapI RAATTY 1 cut(s) 455
XmiI GTMKAC 1 cut(s) 201
XmnI GAANNNNTTC 1 cut(s) 623
XspI CTAG 2 cut(s) 540, 570
ZrmI AGTACT 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.