Rw6G011830

cinnamoyl-CoA reductase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003662
Physical Location & Seq
Forward (+)
21164515 .. 21165479
965 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G011830.1

Sequence Viewer

Length: 624 bp
ATGTCGAAGCAGGGTGAGGTCGTATGCGTCACCGGTGGAAGCGGCTGCATCGGATCATGGGTTGTCCGCCTCCTCCTCGACCGCGGCTACACCGTCCACGCCACCGTCAAAGACCTTATCGACCTCCTCGACTACTATTCCATCTCCGCCGCAGTCCACGGCTGCTCCGGCGTCTTCCACCTCGCCTCTCCTTGCATCGTCGACCAAGTCCATGACCCCCAGAAGGAGCTGTTGGACCCGGTGATTAAAGGAACGCTGAATGTTCTAACGGCGGCTAAGCAGGCCTGCGTGAAGCGCGTGGTGCTCACGTCCTCCATATCGGCCATCACTCCCAGCCCTACTTGGCAGGCCGATGTCGTCAAGAGAGAGGATTGCTGGACTGATGTTGAGTACTGCAAGCAGAAAGGACTGTGGTATCCTTTATCGAAAACTCTGGCGGAGAAAGCTGCGTGGGAATTTGCCGAGGAGAAGGGGTTGGATGTGGTTGTGGTGAATCCGGGGACAGTGATGGGTGATGTTATATCGCCGAGGCTCAATGCTAGCAAAGGTGAGTGCTTTGTTCAGTTTCTCATGAAACCTTTCCTGCATTTGCCTAATTTGTCATCATATTATCTGTCTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

207

Amino Acids

22.7

Weight (kDa)

6.19

Isoelectric Point (pI)

29.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 8 - 172 2.8e-20 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 12 - 169 2.9e-06 NAD(P)H-binding
3Beta_HSD PF01073 40 - 150 2.4e-11 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 55 - 177 2.7e-08 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 201
AccII CGCG 2 cut(s) 84, 297
AciI CCGC 8 cut(s) 42, 67, 82, 84, 147, 150, 272, 437
AclWI GGATC 1 cut(s) 61
AcoI YGGCCR 1 cut(s) 321
AcsI RAATTY 1 cut(s) 455
AcyI GRCGYC 1 cut(s) 171
AfaI GTAC 1 cut(s) 392
AfiI CCNNNNNNNGG 1 cut(s) 223
AgeI ACCGGT 1 cut(s) 32
AjiI CACGTC 1 cut(s) 309
AjuI GAANNNNNNNTTGG 2 cut(s) 215, 247
AluBI AGCT 2 cut(s) 229, 446
AluI AGCT 2 cut(s) 229, 446
Alw21I GWGCWC 1 cut(s) 306
AlwI GGATC 1 cut(s) 61
AoxI GGCC 3 cut(s) 282, 321, 348
ApeKI GCWGC 3 cut(s) 45, 162, 446
ApoI RAATTY 1 cut(s) 455
AsiGI ACCGGT 1 cut(s) 32
Asp700I GAANNNNTTC 1 cut(s) 578
AspLEI GCGC 1 cut(s) 297
AspS9I GGNCC 1 cut(s) 235
AsuC2I CCSGG 2 cut(s) 239, 498
AsuHPI GGTGA 6 cut(s) 22, 26, 253, 502, 524, 560
AsuNHI GCTAGC 1 cut(s) 539
AvaII GGWCC 1 cut(s) 235
BaeI ACNNNNGTAYC 2 cut(s) 398, 431
BbsI GAAGAC 1 cut(s) 166
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 3 cut(s) 32, 149, 433
BccI CCATC 3 cut(s) 149, 332, 502
BceAI ACGGC 2 cut(s) 175, 285
BciVI GTATCC 1 cut(s) 426
BcnI CCSGG 2 cut(s) 239, 498
BfaI CTAG 1 cut(s) 540
BfuI GTATCC 1 cut(s) 426
BisI GCNGC 7 cut(s) 43, 46, 85, 150, 163, 273, 447
BlpI GCTNAGC 1 cut(s) 276
BlsI GCNGC 7 cut(s) 44, 47, 86, 151, 164, 274, 448
BmcAI AGTACT 1 cut(s) 392
Bme1390I CCNGG 2 cut(s) 239, 498
Bme18I GGWCC 1 cut(s) 235
BmgBI CACGTC 1 cut(s) 309
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 1 cut(s) 237
BmrFI CCNGG 2 cut(s) 239, 498
BmsI GCATC 2 cut(s) 57, 204
BmtI GCTAGC 1 cut(s) 543
BpiI GAAGAC 1 cut(s) 166
Bpu1102I GCTNAGC 1 cut(s) 276
BpuMI CCSGG 2 cut(s) 239, 498
BsaHI GRCGYC 1 cut(s) 171
BsaJI CCNNGG 5 cut(s) 82, 157, 462, 497, 527
BsaWI WCCGGW 1 cut(s) 32
BsaXI ACNNNNNCTCC 4 cut(s) 149, 179, 458, 488
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse118I RCCGGY 1 cut(s) 32
BseDI CCNNGG 5 cut(s) 82, 157, 462, 497, 527
BseGI GGATG 1 cut(s) 484
BseLI CCNNNNNNNGG 1 cut(s) 223
BseRI GAGGAG 4 cut(s) 62, 65, 116, 479
BseXI GCAGC 3 cut(s) 32, 149, 433
BseYI CCCAGC 1 cut(s) 332
Bsh1236I CGCG 2 cut(s) 84, 297
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 3 cut(s) 284, 323, 350
BshTI ACCGGT 1 cut(s) 32
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 306
BsiSI CCGG 4 cut(s) 33, 168, 239, 497
BslFI GGGAC 1 cut(s) 514
BslI CCNNNNNNNGG 1 cut(s) 223
BsmFI GGGAC 1 cut(s) 514
BsnI GGCC 3 cut(s) 284, 323, 350
Bsp1286I GDGCHC 1 cut(s) 306
Bsp143I GATC 1 cut(s) 53
Bsp1720I GCTNAGC 1 cut(s) 276
BspACI CCGC 8 cut(s) 42, 67, 82, 84, 147, 150, 272, 437
BspANI GGCC 3 cut(s) 284, 323, 350
BspFNI CGCG 2 cut(s) 84, 297
BspHI TCATGA 1 cut(s) 570
BspLI GGNNCC 1 cut(s) 237
BspOI GCTAGC 1 cut(s) 543
BspPI GGATC 1 cut(s) 61
BsrFI RCCGGY 1 cut(s) 32
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 5 cut(s) 82, 157, 462, 497, 527
BssMI GATC 1 cut(s) 53
BssNI GRCGYC 1 cut(s) 171
Bst4CI ACNGT 4 cut(s) 94, 106, 411, 505
BstACI GRCGYC 1 cut(s) 171
BstC8I GCNNGC 5 cut(s) 282, 286, 348, 398, 541
BstDEI CTNAG 1 cut(s) 276
BstDSI CCRYGG 2 cut(s) 82, 157
BstF5I GGATG 1 cut(s) 484
BstFNI CGCG 2 cut(s) 84, 297
BstHHI GCGC 1 cut(s) 297
BstKTI GATC 1 cut(s) 56
BstMBI GATC 1 cut(s) 53
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 5 cut(s) 168, 281, 294, 301, 443
BstSCI CCNGG 2 cut(s) 237, 496
BstUI CGCG 2 cut(s) 84, 297
BstV1I GCAGC 3 cut(s) 32, 149, 433
BstV2I GAAGAC 1 cut(s) 166
BsuI GTATCC 1 cut(s) 426
BsuRI GGCC 3 cut(s) 284, 323, 350
BtgI CCRYGG 2 cut(s) 82, 157
BtrI CACGTC 1 cut(s) 309
BtsCI GGATG 1 cut(s) 484
BtsIMutI CAGTG 1 cut(s) 510
Cac8I GCNNGC 5 cut(s) 282, 286, 348, 398, 541
CciI TCATGA 1 cut(s) 570
CfoI GCGC 1 cut(s) 297
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 1 cut(s) 235
Cfr42I CCGCGG 1 cut(s) 85
CseI GACGC 2 cut(s) 16, 160
Csp6I GTAC 1 cut(s) 391
CspAI ACCGGT 1 cut(s) 32
CviAII CATG 3 cut(s) 57, 212, 571
CviQI GTAC 1 cut(s) 391
DdeI CTNAG 1 cut(s) 276
DpnI GATC 1 cut(s) 55
DpnII GATC 1 cut(s) 53
EaeI YGGCCR 1 cut(s) 321
EciI GGCGGA 3 cut(s) 56, 136, 452
Eco147I AGGCCT 1 cut(s) 284
Eco47I GGWCC 1 cut(s) 235
FaeI CATG 3 cut(s) 60, 215, 574
FaiI YATR 7 cut(s) 25, 58, 213, 317, 521, 572, 607
FaqI GGGAC 1 cut(s) 514
FatI CATG 3 cut(s) 56, 211, 570
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 7 cut(s) 43, 46, 85, 150, 163, 273, 447
FokI GGATG 1 cut(s) 491
Fsp4HI GCNGC 7 cut(s) 43, 46, 85, 150, 163, 273, 447
FspBI CTAG 1 cut(s) 540
GlaI GCGC 1 cut(s) 296
GluI GCNGC 7 cut(s) 43, 46, 85, 150, 163, 273, 447
GsaI CCCAGC 1 cut(s) 336
HaeIII GGCC 3 cut(s) 284, 323, 350
HapII CCGG 4 cut(s) 33, 168, 239, 497
HgaI GACGC 2 cut(s) 16, 160
HhaI GCGC 1 cut(s) 297
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 3 cut(s) 60, 215, 574
Hin6I GCGC 1 cut(s) 295
HinP1I GCGC 1 cut(s) 295
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 1 cut(s) 493
HpaII CCGG 4 cut(s) 33, 168, 239, 497
HphI GGTGA 6 cut(s) 22, 26, 253, 502, 524, 560
Hpy166II GTNNAC 3 cut(s) 97, 157, 202
Hpy188I TCNGA 1 cut(s) 53
Hpy188III TCNNGA 2 cut(s) 361, 571
Hpy8I GTNNAC 3 cut(s) 97, 157, 202
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 2 cut(s) 217, 463
HpyCH4III ACNGT 4 cut(s) 94, 106, 411, 505
HpyCH4IV ACGT 1 cut(s) 308
HpyCH4V TGCA 4 cut(s) 48, 195, 396, 586
HpyF10VI GCNNNNNNNGC 5 cut(s) 168, 281, 294, 301, 443
HpyF3I CTNAG 1 cut(s) 276
HpySE526I ACGT 1 cut(s) 308
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 3 cut(s) 60, 215, 574
HspAI GCGC 1 cut(s) 295
KspI CCGCGG 1 cut(s) 85
Kzo9I GATC 1 cut(s) 53
LmnI GCTCC 2 cut(s) 170, 226
Lsp1109I GCAGC 3 cut(s) 32, 149, 433
LweI GCATC 2 cut(s) 57, 204
MaeI CTAG 1 cut(s) 540
MaeII ACGT 1 cut(s) 308
MaeIII GTNAC 1 cut(s) 28
MalI GATC 1 cut(s) 55
MboI GATC 1 cut(s) 53
MboII GAAGA 1 cut(s) 166
MhlI GDGCHC 1 cut(s) 306
MluCI AATT 2 cut(s) 455, 595
MmeI TCCRAC 2 cut(s) 213, 456
MroXI GAANNNNTTC 1 cut(s) 578
MseI TTAA 2 cut(s) 246, 622
MspA1I CMGCKG 1 cut(s) 84
MspI CCGG 4 cut(s) 33, 168, 239, 497
MspR9I CCNGG 2 cut(s) 239, 498
MvnI CGCG 2 cut(s) 84, 297
MwoI GCNNNNNNNGC 5 cut(s) 168, 281, 294, 301, 443
NciI CCSGG 2 cut(s) 239, 498
NdeII GATC 1 cut(s) 53
NheI GCTAGC 1 cut(s) 539
NlaIII CATG 3 cut(s) 60, 215, 574
NlaIV GGNNCC 1 cut(s) 237
NmeAIII GCCGAG 2 cut(s) 487, 552
NmuCI GTSAC 1 cut(s) 28
PagI TCATGA 1 cut(s) 570
PceI AGGCCT 1 cut(s) 284
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PdmI GAANNNNTTC 1 cut(s) 578
PfeI GAWTC 1 cut(s) 493
PflFI GACNNNGTC 1 cut(s) 206
PinAI ACCGGT 1 cut(s) 32
PkrI GCNGC 7 cut(s) 44, 47, 86, 151, 164, 274, 448
PspFI CCCAGC 1 cut(s) 332
PspN4I GGNNCC 1 cut(s) 237
PspPI GGNCC 1 cut(s) 235
PsyI GACNNNGTC 1 cut(s) 206
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
SacII CCGCGG 1 cut(s) 85
SalI GTCGAC 1 cut(s) 200
SaqAI TTAA 2 cut(s) 246, 622
SatI GCNGC 7 cut(s) 43, 46, 85, 150, 163, 273, 447
Sau3AI GATC 1 cut(s) 53
Sau96I GGNCC 1 cut(s) 235
ScaI AGTACT 1 cut(s) 392
ScrFI CCNGG 2 cut(s) 239, 498
SduI GDGCHC 1 cut(s) 306
SetI ASST 9 cut(s) 21, 117, 126, 183, 231, 311, 448, 550, 580
SfaNI GCATC 2 cut(s) 57, 204
Sfr303I CCGCGG 1 cut(s) 85
SgrAI CRCCGGYG 1 cut(s) 32
SgrBI CCGCGG 1 cut(s) 85
SinI GGWCC 1 cut(s) 235
Sse9I AATT 2 cut(s) 455, 595
SseBI AGGCCT 1 cut(s) 284
SsiI CCGC 8 cut(s) 42, 67, 82, 84, 147, 150, 272, 437
SspMI CTAG 1 cut(s) 540
StuI AGGCCT 1 cut(s) 284
StyD4I CCNGG 2 cut(s) 237, 496
TaaI ACNGT 4 cut(s) 94, 106, 411, 505
TaiI ACGT 1 cut(s) 311
TaqI TCGA 6 cut(s) 5, 78, 120, 129, 201, 425
TasI AATT 2 cut(s) 455, 595
TatI WGTACW 1 cut(s) 390
TauI GCSGC 4 cut(s) 45, 87, 152, 275
TfiI GAWTC 1 cut(s) 493
Tru1I TTAA 2 cut(s) 246, 622
Tru9I TTAA 2 cut(s) 246, 622
TscAI CASTG 1 cut(s) 510
TseFI GTSAC 1 cut(s) 28
TseI GCWGC 3 cut(s) 45, 162, 446
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 1 cut(s) 587
TspRI CASTG 1 cut(s) 510
Tth111I GACNNNGTC 1 cut(s) 206
VpaK11BI GGWCC 1 cut(s) 235
XapI RAATTY 1 cut(s) 455
XmiI GTMKAC 1 cut(s) 201
XmnI GAANNNNTTC 1 cut(s) 578
XspI CTAG 1 cut(s) 540
ZrmI AGTACT 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.