MD11G1064000.v1.1

cinnamoyl-CoA reductase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003662
Physical Location & Seq
Forward (+)
5530350 .. 5533430
3081 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1064000.v1.1.491

Sequence Viewer

Length: 975 bp
ATGTCGAAGCAGGGTGAGGTCGTATGCGTTACCGGTGGAAGCGGCTGCATCGGATCCTGGCTCGTCCGTCTCCTCCTCCACCGTGATTACACCGTCCACGCCACCGTCAAGGATCTCAAGGACGATGGCGAGACGAAGCATCTAGAAGCGTTAGTAGAGGGGGCAGAGTCGCGCCTCCGTCTCTTCCAGATCGACCTCCTCGACTACAACTCCATACTCGCCGCCGTCAATGGTTGCTCCGGGGTCTTCCACCTCGCTTCTCCCTGCATCATCGATACAGTCCACGACCCCGAGAAGGAGCTCCTGGACCCGGCGATCAAAGGAACGCTTAATGTTCTGACGGCAGCGAAGAAGGCTGGGATCAGCCGTGTGGTGCTGACGTCATCCTCATGCGCCATCACTCCCAGCCCAAGCTGGCCGTCCGATAAGGTCAAGGGCGAGGATTGCTGGACAGACATTGACTACTGCAAGCAGAAGGGATTGTGGTATCCATTATCGAAAACGCTGGCGGAGAAAGCTGCGTGGGAATTTGCCAAGGAGAAGGGGCTGGATGTCGTTGTGGTGAATCCGGGCGCGGTGATGGGCCCTGTTATCTCGCCCAGGCTCAATTCTAGCATGTTATTGCTCCTTCGCATTCTTGAGGGTTGCACTGAAACATATGAGGACTTTTTTATGGGATCTGTGCATTTTAAAGATGTAGCTCTCGCACACATTTTAGTGTATGAGAACAAATCAGCGACCGGTAGGCACTTGTGTGTGGAAGCTATATCACGTTATGGTGACTTTGTGGCAAAGGTTGCTGAACTTTACCCTGAGTACAAGGTTCACAGTTTGCCAAAGGATACCCAACCCGGCTTGCTGAGGGAAAAGAGCGGAGCGAAGAAGCTAATGAACTTGGGTTTAGAATTCATTCCCATGGATCAAATTATCAAGGATGGTGTTGAGAGCCTAAAGAGCAAGGGATTTATTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

325

Amino Acids

35.48

Weight (kDa)

6.16

Isoelectric Point (pI)

26.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 129 2.2e-10 NmrA-like family
Epimerase PF01370 8 - 246 4e-26 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 8 - 138 4.3e-09 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 10 - 240 1.1e-18 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 130 1.7e-16 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 200 2.8e-09 Male sterility protein
NAD_binding_10 PF13460 12 - 192 9.7e-12 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 383
AccBSI CCGCTC 1 cut(s) 873
AccII CGCG 2 cut(s) 172, 575
AciI CCGC 5 cut(s) 42, 222, 509, 575, 873
AclWI GGATC 6 cut(s) 48, 61, 120, 368, 685, 927
AcoI YGGCCR 1 cut(s) 416
AcsI RAATTY 2 cut(s) 527, 905
AcyI GRCGYC 1 cut(s) 380
AfaI GTAC 1 cut(s) 818
AfiI CCNNNNNNNGG 2 cut(s) 295, 310
AgeI ACCGGT 2 cut(s) 32, 740
AjnI CCWGG 3 cut(s) 56, 303, 599
AleI CACNNNNGTG 1 cut(s) 753
AluBI AGCT 6 cut(s) 301, 414, 518, 701, 764, 886
AluI AGCT 6 cut(s) 301, 414, 518, 701, 764, 886
Alw21I GWGCWC 1 cut(s) 303
Alw26I GTCTC 3 cut(s) 74, 125, 185
AlwI GGATC 6 cut(s) 48, 61, 120, 368, 685, 927
Ama87I CYCGRG 1 cut(s) 290
AoxI GGCC 2 cut(s) 416, 583
ApaI GGGCCC 1 cut(s) 587
ApeKI GCWGC 3 cut(s) 45, 344, 518
ApoI RAATTY 2 cut(s) 527, 905
AsiGI ACCGGT 2 cut(s) 32, 740
Asp700I GAANNNNTTC 1 cut(s) 909
AspLEI GCGC 3 cut(s) 174, 395, 575
AspS9I GGNCC 3 cut(s) 307, 583, 584
AsuC2I CCSGG 4 cut(s) 241, 311, 570, 852
AsuHPI GGTGA 4 cut(s) 26, 574, 589, 791
AvaI CYCGRG 1 cut(s) 290
AvaII GGWCC 1 cut(s) 307
BaeGI GKGCMC 1 cut(s) 587
BamHI GGATCC 1 cut(s) 53
BanII GRGCYC 2 cut(s) 303, 587
BbsI GAAGAC 1 cut(s) 238
Bbv12I GWGCWC 1 cut(s) 303
BbvCI CCTCAGC 1 cut(s) 860
BbvI GCAGC 3 cut(s) 32, 356, 505
BccI CCATC 4 cut(s) 119, 404, 574, 929
BceAI ACGGC 4 cut(s) 209, 351, 357, 403
BciT130I CCWGG 3 cut(s) 58, 305, 601
BciVI GTATCC 2 cut(s) 498, 835
BcnI CCSGG 4 cut(s) 241, 311, 570, 852
BcoDI GTCTC 3 cut(s) 74, 125, 185
BfaI CTAG 2 cut(s) 143, 612
BfuI GTATCC 2 cut(s) 498, 835
BisI GCNGC 5 cut(s) 43, 46, 222, 345, 519
BlsI GCNGC 5 cut(s) 44, 47, 223, 346, 520
Bme1390I CCNGG 7 cut(s) 58, 241, 305, 311, 570, 601, 852
Bme18I GGWCC 1 cut(s) 307
BmeT110I CYCGRG 1 cut(s) 290
BmgT120I GGNCC 3 cut(s) 307, 583, 584
BmiI GGNNCC 3 cut(s) 55, 309, 585
BmrFI CCNGG 7 cut(s) 58, 241, 305, 311, 570, 601, 852
BmsI GCATC 3 cut(s) 57, 148, 276
BpiI GAAGAC 1 cut(s) 238
Bpu10I CCTNAGC 1 cut(s) 860
BpuEI CTTGAG 2 cut(s) 101, 659
BpuMI CCSGG 4 cut(s) 241, 311, 570, 852
Bsa29I ATCGAT 1 cut(s) 273
BsaHI GRCGYC 1 cut(s) 380
BsaJI CCNNGG 4 cut(s) 240, 534, 599, 915
BsaWI WCCGGW 2 cut(s) 32, 740
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 2 cut(s) 295, 310
Bse118I RCCGGY 2 cut(s) 32, 740
BseBI CCWGG 3 cut(s) 58, 305, 601
BseCI ATCGAT 1 cut(s) 273
BseDI CCNNGG 4 cut(s) 240, 534, 599, 915
BseGI GGATG 3 cut(s) 383, 556, 940
BseLI CCNNNNNNNGG 2 cut(s) 295, 310
BseMII CTCAG 2 cut(s) 804, 851
BseRI GAGGAG 3 cut(s) 62, 65, 188
BseSI GKGCMC 1 cut(s) 587
BseXI GCAGC 3 cut(s) 32, 356, 505
BseYI CCCAGC 2 cut(s) 356, 404
Bsh1236I CGCG 2 cut(s) 172, 575
Bsh1285I CGRYCG 1 cut(s) 741
BshFI GGCC 2 cut(s) 418, 585
BshTI ACCGGT 2 cut(s) 32, 740
BshVI ATCGAT 1 cut(s) 273
BsiEI CGRYCG 1 cut(s) 741
BsiHKAI GWGCWC 1 cut(s) 303
BsiHKCI CYCGRG 1 cut(s) 290
BsiSI CCGG 6 cut(s) 33, 240, 311, 569, 741, 852
BslI CCNNNNNNNGG 2 cut(s) 295, 310
BsmAI GTCTC 3 cut(s) 74, 125, 185
BsmBI CGTCTC 3 cut(s) 74, 125, 185
BsmI GAATGC 1 cut(s) 633
BsnI GGCC 2 cut(s) 418, 585
BsoBI CYCGRG 1 cut(s) 290
Bsp120I GGGCCC 1 cut(s) 583
Bsp1286I GDGCHC 2 cut(s) 303, 587
Bsp143I GATC 7 cut(s) 53, 112, 189, 315, 360, 677, 919
Bsp19I CCATGG 1 cut(s) 915
BspACI CCGC 5 cut(s) 42, 222, 509, 575, 873
BspANI GGCC 2 cut(s) 418, 585
BspCNI CTCAG 2 cut(s) 805, 852
BspDI ATCGAT 1 cut(s) 273
BspFNI CGCG 2 cut(s) 172, 575
BspLI GGNNCC 3 cut(s) 55, 309, 585
BspPI GGATC 6 cut(s) 48, 61, 120, 368, 685, 927
BsrBI CCGCTC 1 cut(s) 873
BsrFI RCCGGY 2 cut(s) 32, 740
BssAI RCCGGY 2 cut(s) 32, 740
BssECI CCNNGG 4 cut(s) 240, 534, 599, 915
BssMI GATC 7 cut(s) 53, 112, 189, 315, 360, 677, 919
BssNI GRCGYC 1 cut(s) 380
BssT1I CCWWGG 2 cut(s) 534, 915
Bst2UI CCWGG 3 cut(s) 58, 305, 601
Bst4CI ACNGT 5 cut(s) 83, 94, 106, 280, 830
Bst6I CTCTTC 1 cut(s) 188
BstACI GRCGYC 1 cut(s) 380
BstAPI GCANNNNNTGC 1 cut(s) 797
BstC8I GCNNGC 4 cut(s) 416, 470, 507, 857
BstDEI CTNAG 2 cut(s) 813, 860
BstDSI CCRYGG 1 cut(s) 915
BstF5I GGATG 3 cut(s) 383, 556, 940
BstFNI CGCG 2 cut(s) 172, 575
BstHHI GCGC 3 cut(s) 174, 395, 575
BstKTI GATC 7 cut(s) 56, 115, 192, 318, 363, 680, 922
BstMAI GTCTC 3 cut(s) 74, 125, 185
BstMBI GATC 7 cut(s) 53, 112, 189, 315, 360, 677, 919
BstMCI CGRYCG 1 cut(s) 741
BstMWI GCNNNNNNNGC 5 cut(s) 353, 444, 515, 797, 954
BstNI CCWGG 3 cut(s) 58, 305, 601
BstNSI RCATGY 1 cut(s) 619
BstSCI CCNGG 7 cut(s) 56, 239, 303, 309, 568, 599, 850
BstSLI GKGCMC 1 cut(s) 587
BstUI CGCG 2 cut(s) 172, 575
BstV1I GCAGC 3 cut(s) 32, 356, 505
BstV2I GAAGAC 1 cut(s) 238
BstX2I RGATCY 3 cut(s) 53, 112, 677
BstYI RGATCY 3 cut(s) 53, 112, 677
Bsu15I ATCGAT 1 cut(s) 273
BsuI GTATCC 2 cut(s) 498, 835
BsuRI GGCC 2 cut(s) 418, 585
BsuTUI ATCGAT 1 cut(s) 273
BtgI CCRYGG 1 cut(s) 915
BtsCI GGATG 3 cut(s) 383, 556, 940
BtsIMutI CAGTG 1 cut(s) 648
Cac8I GCNNGC 4 cut(s) 416, 470, 507, 857
CfoI GCGC 3 cut(s) 174, 395, 575
Cfr10I RCCGGY 2 cut(s) 32, 740
Cfr13I GGNCC 3 cut(s) 307, 583, 584
ClaI ATCGAT 1 cut(s) 273
Csp6I GTAC 1 cut(s) 817
CspAI ACCGGT 2 cut(s) 32, 740
CviAII CATG 3 cut(s) 390, 616, 916
CviQI GTAC 1 cut(s) 817
DdeI CTNAG 2 cut(s) 813, 860
DpnI GATC 7 cut(s) 55, 114, 191, 317, 362, 679, 921
DpnII GATC 7 cut(s) 53, 112, 189, 315, 360, 677, 919
DraI TTTAAA 1 cut(s) 691
EaeI YGGCCR 1 cut(s) 416
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
EciI GGCGGA 1 cut(s) 524
Ecl136II GAGCTC 1 cut(s) 301
Eco130I CCWWGG 2 cut(s) 534, 915
Eco24I GRGCYC 2 cut(s) 303, 587
Eco47I GGWCC 1 cut(s) 307
Eco53kI GAGCTC 1 cut(s) 301
Eco88I CYCGRG 1 cut(s) 290
EcoICRI GAGCTC 1 cut(s) 301
EcoO109I RGGNCCY 1 cut(s) 584
EcoRI GAATTC 1 cut(s) 905
EcoRII CCWGG 3 cut(s) 56, 303, 599
EcoT14I CCWWGG 2 cut(s) 534, 915
EcoT38I GRGCYC 2 cut(s) 303, 587
ErhI CCWWGG 2 cut(s) 534, 915
Esp3I CGTCTC 3 cut(s) 74, 125, 185
FaeI CATG 3 cut(s) 393, 619, 919
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FatI CATG 3 cut(s) 389, 615, 915
FauNDI CATATG 1 cut(s) 658
Fnu4HI GCNGC 5 cut(s) 43, 46, 222, 345, 519
FokI GGATG 3 cut(s) 370, 563, 947
FriOI GRGCYC 2 cut(s) 303, 587
Fsp4HI GCNGC 5 cut(s) 43, 46, 222, 345, 519
FspBI CTAG 2 cut(s) 143, 612
GlaI GCGC 3 cut(s) 173, 394, 574
GluI GCNGC 5 cut(s) 43, 46, 222, 345, 519
GsaI CCCAGC 2 cut(s) 360, 408
HaeIII GGCC 2 cut(s) 418, 585
HapII CCGG 6 cut(s) 33, 240, 311, 569, 741, 852
HhaI GCGC 3 cut(s) 174, 395, 575
Hin1I GRCGYC 1 cut(s) 380
Hin1II CATG 3 cut(s) 393, 619, 919
Hin6I GCGC 3 cut(s) 172, 393, 573
HinP1I GCGC 3 cut(s) 172, 393, 573
HinfI GANTC 2 cut(s) 167, 565
HpaII CCGG 6 cut(s) 33, 240, 311, 569, 741, 852
HphI GGTGA 4 cut(s) 26, 574, 589, 791
Hpy166II GTNNAC 3 cut(s) 97, 283, 826
Hpy188I TCNGA 3 cut(s) 53, 339, 424
Hpy188III TCNNGA 3 cut(s) 143, 187, 638
Hpy8I GTNNAC 3 cut(s) 97, 283, 826
HpyAV CCTTC 5 cut(s) 289, 346, 469, 535, 638
HpyCH4III ACNGT 5 cut(s) 83, 94, 106, 280, 830
HpyCH4IV ACGT 2 cut(s) 380, 772
HpyCH4V TGCA 5 cut(s) 48, 267, 468, 648, 685
HpyF10VI GCNNNNNNNGC 5 cut(s) 353, 444, 515, 797, 954
HpyF3I CTNAG 2 cut(s) 813, 860
HpySE526I ACGT 2 cut(s) 380, 772
Hsp92I GRCGYC 1 cut(s) 380
Hsp92II CATG 3 cut(s) 393, 619, 919
HspAI GCGC 3 cut(s) 172, 393, 573
Kzo9I GATC 7 cut(s) 53, 112, 189, 315, 360, 677, 919
LmnI GCTCC 5 cut(s) 242, 298, 306, 630, 875
Lsp1109I GCAGC 3 cut(s) 32, 356, 505
LweI GCATC 3 cut(s) 57, 148, 276
MaeI CTAG 2 cut(s) 143, 612
MaeII ACGT 2 cut(s) 380, 772
MaeIII GTNAC 2 cut(s) 28, 779
MalI GATC 7 cut(s) 55, 114, 191, 317, 362, 679, 921
MbiI CCGCTC 1 cut(s) 873
MboI GATC 7 cut(s) 53, 112, 189, 315, 360, 677, 919
MboII GAAGA 4 cut(s) 175, 238, 361, 892
MflI RGATCY 3 cut(s) 53, 112, 677
MhlI GDGCHC 2 cut(s) 303, 587
MluCI AATT 4 cut(s) 527, 607, 905, 924
MlyI GAGTC 1 cut(s) 176
MroXI GAANNNNTTC 1 cut(s) 909
MseI TTAA 2 cut(s) 330, 690
MslI CAYNNNNRTG 4 cut(s) 388, 716, 753, 914
MspI CCGG 6 cut(s) 33, 240, 311, 569, 741, 852
MspR9I CCNGG 7 cut(s) 58, 241, 305, 311, 570, 601, 852
Mva1269I GAATGC 1 cut(s) 633
MvaI CCWGG 3 cut(s) 58, 305, 601
MvnI CGCG 2 cut(s) 172, 575
MwoI GCNNNNNNNGC 5 cut(s) 353, 444, 515, 797, 954
NciI CCSGG 4 cut(s) 241, 311, 570, 852
NcoI CCATGG 1 cut(s) 915
NdeI CATATG 1 cut(s) 658
NdeII GATC 7 cut(s) 53, 112, 189, 315, 360, 677, 919
NlaIII CATG 3 cut(s) 393, 619, 919
NlaIV GGNNCC 3 cut(s) 55, 309, 585
NmuCI GTSAC 1 cut(s) 779
NspI RCATGY 1 cut(s) 619
OliI CACNNNNGTG 1 cut(s) 753
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 1 cut(s) 633
PdmI GAANNNNTTC 1 cut(s) 909
PfeI GAWTC 1 cut(s) 565
PfoI TCCNGGA 1 cut(s) 303
PinAI ACCGGT 2 cut(s) 32, 740
PkrI GCNGC 5 cut(s) 44, 47, 223, 346, 520
PleI GAGTC 1 cut(s) 175
PpsI GAGTC 1 cut(s) 175
Psp124BI GAGCTC 1 cut(s) 303
Psp6I CCWGG 3 cut(s) 56, 303, 599
PspFI CCCAGC 2 cut(s) 356, 404
PspGI CCWGG 3 cut(s) 56, 303, 599
PspN4I GGNNCC 3 cut(s) 55, 309, 585
PspOMI GGGCCC 1 cut(s) 583
PspPI GGNCC 3 cut(s) 307, 583, 584
PsuI RGATCY 3 cut(s) 53, 112, 677
RsaI GTAC 1 cut(s) 818
RsaNI GTAC 1 cut(s) 817
RseI CAYNNNNRTG 4 cut(s) 388, 716, 753, 914
SacI GAGCTC 1 cut(s) 303
SaqAI TTAA 2 cut(s) 330, 690
SatI GCNGC 5 cut(s) 43, 46, 222, 345, 519
Sau3AI GATC 7 cut(s) 53, 112, 189, 315, 360, 677, 919
Sau96I GGNCC 3 cut(s) 307, 583, 584
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 7 cut(s) 58, 241, 305, 311, 570, 601, 852
SduI GDGCHC 2 cut(s) 303, 587
SfaNI GCATC 3 cut(s) 57, 148, 276
SinI GGWCC 1 cut(s) 307
SmiMI CAYNNNNRTG 4 cut(s) 388, 716, 753, 914
SmlI CTYRAG 2 cut(s) 116, 638
SmoI CTYRAG 2 cut(s) 116, 638
Sse9I AATT 4 cut(s) 527, 607, 905, 924
SsiI CCGC 5 cut(s) 42, 222, 509, 575, 873
SspMI CTAG 2 cut(s) 143, 612
SstI GAGCTC 1 cut(s) 303
StyD4I CCNGG 7 cut(s) 56, 239, 303, 309, 568, 599, 850
StyI CCWWGG 2 cut(s) 534, 915
TaaI ACNGT 5 cut(s) 83, 94, 106, 280, 830
TaiI ACGT 2 cut(s) 383, 775
TaqI TCGA 5 cut(s) 5, 192, 201, 273, 497
TasI AATT 4 cut(s) 527, 607, 905, 924
TatI WGTACW 1 cut(s) 816
TauI GCSGC 2 cut(s) 45, 224
TfiI GAWTC 1 cut(s) 565
Tru1I TTAA 2 cut(s) 330, 690
Tru9I TTAA 2 cut(s) 330, 690
TscAI CASTG 1 cut(s) 655
TseFI GTSAC 1 cut(s) 779
TseI GCWGC 3 cut(s) 45, 344, 518
Tsp45I GTSAC 1 cut(s) 779
TspDTI ATGAA 3 cut(s) 898, 905, 960
TspGWI ACGGA 2 cut(s) 56, 167
TspRI CASTG 1 cut(s) 655
VpaK11BI GGWCC 1 cut(s) 307
XapI RAATTY 2 cut(s) 527, 905
XbaI TCTAGA 1 cut(s) 142
XceI RCATGY 1 cut(s) 619
XmnI GAANNNNTTC 1 cut(s) 909
XspI CTAG 2 cut(s) 143, 612
ZraI GACGTC 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.