AT5G63000

Tim17/Tim22/Tim23/Pmp24 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006145
Physical Location & Seq
Reverse (-)
25280012 .. 25281652
1641 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G63000.1

Sequence Viewer

Length: 606 bp
ATGGAAGCGGTAGAAAAACCCGAAGAAACAATCCCAGGGAATTCTTCAAGATTAGCTGGAAATCCCAACTGGGGAACAGCAACTGTTGTTGGAGTATTTGCAGGATTGCTATATGGAGGGAGCAAAGAAGCTTCTGCTTCTGTGAGCAAAGATGCAGAAGTGATGTTGAAGATGGGTAGTACACAGGATAAGCGTGAACAACACAGGTTGATGCGAGATGCAATGGAGAAAAGGTTCATCCGAGTAACTCGTGGCTCCCTCATTGGAGGGATGCGTCTTGGAATGTTCACTGCTTCCTTCTTTAGTCTACAAAACTTTTTAGCTGAGACCCGAGGAGTGCATGATGTTTTCAATGTGGTTGGAGCTGGTTCAGCTACTGCTGCAGTTTTTGGCCTAATCATGCCGGGGTCTCTTGCTTGGCGTGCGCGGAATGTGTTGCTTGGTTCAGTTCTTGGAGCCACAGTGTGCTTCCCTCTTGGGTGGGTACAACTGAAGCTCATGAAGAAAGCAAACGAAGGCAATAACGTTGACACAGGCCACCACGGTGAGGTCACGAGTGGTGTTGGTGCAGCCATTGAGAGACTCGAACAACAGTTGAGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

201

Amino Acids

21.52

Weight (kDa)

9.6

Isoelectric Point (pI)

18.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 25 - 154 4.3e-08 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 307
AccII CGCG 1 cut(s) 427
AciI CCGC 2 cut(s) 8, 427
AclI AACGTT 1 cut(s) 525
AcsI RAATTY 1 cut(s) 40
AcuI CTGAAG 1 cut(s) 512
AdeI CACNNNGTG 1 cut(s) 465
AfaI GTAC 2 cut(s) 181, 486
AfiI CCNNNNNNNGG 2 cut(s) 71, 547
AgsI TTSAA 3 cut(s) 48, 169, 352
AjnI CCWGG 1 cut(s) 34
AleI CACNNNNGTG 1 cut(s) 543
AloI GAACNNNNNNTCC 2 cut(s) 218, 250
AluBI AGCT 6 cut(s) 56, 131, 323, 365, 374, 496
AluI AGCT 6 cut(s) 56, 131, 323, 365, 374, 496
Alw26I GTCTC 3 cut(s) 320, 414, 574
AlwNI CAGNNNCTG 2 cut(s) 83, 377
Ama87I CYCGRG 1 cut(s) 330
AoxI GGCC 2 cut(s) 391, 535
ApeKI GCWGC 2 cut(s) 380, 569
ApoI RAATTY 1 cut(s) 40
Asp700I GAANNNNTTC 1 cut(s) 233
AspLEI GCGC 1 cut(s) 427
AsuC2I CCSGG 1 cut(s) 405
AsuHPI GGTGA 1 cut(s) 557
AvaI CYCGRG 1 cut(s) 330
BauI CACGAG 2 cut(s) 249, 553
BbvI GCAGC 2 cut(s) 367, 581
BccI CCATC 1 cut(s) 166
BciT130I CCWGG 1 cut(s) 36
BcnI CCSGG 1 cut(s) 405
BcoDI GTCTC 3 cut(s) 320, 414, 574
BfmI CTRYAG 1 cut(s) 381
BisI GCNGC 2 cut(s) 381, 570
BlsI GCNGC 2 cut(s) 382, 571
Bme1390I CCNGG 2 cut(s) 36, 405
BmeT110I CYCGRG 1 cut(s) 330
BmiI GGNNCC 2 cut(s) 256, 457
BmrFI CCNGG 2 cut(s) 36, 405
BmrI ACTGGG 1 cut(s) 79
BmsI GCATC 4 cut(s) 142, 201, 208, 261
BmuI ACTGGG 1 cut(s) 79
BpuMI CCSGG 1 cut(s) 405
BsaI GGTCTC 2 cut(s) 320, 414
BsaJI CCNNGG 5 cut(s) 34, 35, 331, 404, 541
BsaXI ACNNNNNCTCC 4 cut(s) 218, 248, 447, 477
Bsc4I CCNNNNNNNGG 2 cut(s) 71, 547
Bse1I ACTGG 1 cut(s) 74
Bse3DI GCAATG 1 cut(s) 228
BseBI CCWGG 1 cut(s) 36
BseDI CCNNGG 5 cut(s) 34, 35, 331, 404, 541
BseGI GGATG 2 cut(s) 237, 276
BseLI CCNNNNNNNGG 2 cut(s) 71, 547
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 1 cut(s) 315
BseNI ACTGG 1 cut(s) 74
BseRI GAGGAG 1 cut(s) 348
BseXI GCAGC 2 cut(s) 367, 581
BsgI GTGCAG 1 cut(s) 588
Bsh1236I CGCG 1 cut(s) 427
BshFI GGCC 2 cut(s) 393, 537
BsiHKCI CYCGRG 1 cut(s) 330
BsiSI CCGG 1 cut(s) 404
BslI CCNNNNNNNGG 2 cut(s) 71, 547
BsmAI GTCTC 3 cut(s) 320, 414, 574
BsnI GGCC 2 cut(s) 393, 537
Bso31I GGTCTC 2 cut(s) 320, 414
BsoBI CYCGRG 1 cut(s) 330
BspACI CCGC 2 cut(s) 8, 427
BspANI GGCC 2 cut(s) 393, 537
BspCNI CTCAG 1 cut(s) 316
BspFNI CGCG 1 cut(s) 427
BspHI TCATGA 1 cut(s) 498
BspLI GGNNCC 2 cut(s) 256, 457
BspMAI CTGCAG 1 cut(s) 385
BspTNI GGTCTC 2 cut(s) 320, 414
BsrDI GCAATG 1 cut(s) 228
BsrI ACTGG 1 cut(s) 74
BssECI CCNNGG 5 cut(s) 34, 35, 331, 404, 541
BssSI CACGAG 2 cut(s) 249, 553
Bst2BI CACGAG 2 cut(s) 249, 553
Bst2UI CCWGG 1 cut(s) 36
Bst4CI ACNGT 4 cut(s) 85, 463, 545, 594
BstC8I GCNNGC 1 cut(s) 423
BstDEI CTNAG 1 cut(s) 324
BstDSI CCRYGG 1 cut(s) 541
BstF5I GGATG 2 cut(s) 237, 276
BstFNI CGCG 1 cut(s) 427
BstHHI GCGC 1 cut(s) 427
BstMAI GTCTC 3 cut(s) 320, 414, 574
BstMWI GCNNNNNNNGC 3 cut(s) 371, 380, 422
BstNI CCWGG 1 cut(s) 36
BstSCI CCNGG 2 cut(s) 34, 403
BstSFI CTRYAG 1 cut(s) 381
BstUI CGCG 1 cut(s) 427
BstV1I GCAGC 2 cut(s) 367, 581
BsuRI GGCC 2 cut(s) 393, 537
BtgI CCRYGG 1 cut(s) 541
BtsCI GGATG 2 cut(s) 237, 276
BtsI GCAGTG 1 cut(s) 288
BtsIMutI CAGTG 2 cut(s) 288, 468
Cac8I GCNNGC 1 cut(s) 423
CaiI CAGNNNCTG 2 cut(s) 83, 377
CciI TCATGA 1 cut(s) 498
CfoI GCGC 1 cut(s) 427
CseI GACGC 1 cut(s) 263
Csp6I GTAC 2 cut(s) 180, 485
CviAII CATG 3 cut(s) 341, 400, 499
CviQI GTAC 2 cut(s) 180, 485
DdeI CTNAG 1 cut(s) 324
DraIII CACNNNGTG 1 cut(s) 465
Eco31I GGTCTC 2 cut(s) 320, 414
Eco57I CTGAAG 1 cut(s) 512
Eco88I CYCGRG 1 cut(s) 330
EcoRI GAATTC 1 cut(s) 40
EcoRII CCWGG 1 cut(s) 34
FaeI CATG 3 cut(s) 344, 403, 502
FaiI YATR 5 cut(s) 112, 114, 342, 401, 500
FatI CATG 3 cut(s) 340, 399, 498
FblI GTMKAC 1 cut(s) 307
Fnu4HI GCNGC 2 cut(s) 381, 570
FokI GGATG 2 cut(s) 224, 283
Fsp4HI GCNGC 2 cut(s) 381, 570
GlaI GCGC 1 cut(s) 426
GluI GCNGC 2 cut(s) 381, 570
HaeIII GGCC 2 cut(s) 393, 537
HapII CCGG 1 cut(s) 404
HgaI GACGC 1 cut(s) 263
HhaI GCGC 1 cut(s) 427
Hin1II CATG 3 cut(s) 344, 403, 502
Hin6I GCGC 1 cut(s) 425
HinP1I GCGC 1 cut(s) 425
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HindIII AAGCTT 1 cut(s) 129
HinfI GANTC 1 cut(s) 582
HpaII CCGG 1 cut(s) 404
HphI GGTGA 1 cut(s) 557
Hpy166II GTNNAC 5 cut(s) 182, 197, 288, 308, 529
Hpy188I TCNGA 1 cut(s) 242
Hpy188III TCNNGA 3 cut(s) 48, 499, 553
Hpy8I GTNNAC 5 cut(s) 182, 197, 288, 308, 529
HpyAV CCTTC 2 cut(s) 307, 509
HpyCH4III ACNGT 4 cut(s) 85, 463, 545, 594
HpyCH4IV ACGT 1 cut(s) 525
HpyCH4V TGCA 6 cut(s) 101, 155, 221, 340, 383, 569
HpyF10VI GCNNNNNNNGC 3 cut(s) 371, 380, 422
HpyF3I CTNAG 1 cut(s) 324
HpySE526I ACGT 1 cut(s) 525
Hsp92II CATG 3 cut(s) 344, 403, 502
HspAI GCGC 1 cut(s) 425
LmnI GCTCC 4 cut(s) 120, 260, 362, 455
Lsp1109I GCAGC 2 cut(s) 367, 581
LweI GCATC 4 cut(s) 142, 201, 208, 261
MaeII ACGT 1 cut(s) 525
MaeIII GTNAC 2 cut(s) 244, 550
MboII GAAGA 4 cut(s) 35, 36, 181, 514
MluCI AATT 1 cut(s) 40
MlyI GAGTC 1 cut(s) 576
MmeI TCCRAC 2 cut(s) 70, 340
MnlI CCTC 6 cut(s) 110, 260, 269, 326, 483, 541
MroXI GAANNNNTTC 1 cut(s) 233
MslI CAYNNNNRTG 1 cut(s) 543
MspI CCGG 1 cut(s) 404
MspR9I CCNGG 2 cut(s) 36, 405
MvaI CCWGG 1 cut(s) 36
MvnI CGCG 1 cut(s) 427
MwoI GCNNNNNNNGC 3 cut(s) 371, 380, 422
NciI CCSGG 1 cut(s) 405
NlaIII CATG 3 cut(s) 344, 403, 502
NlaIV GGNNCC 2 cut(s) 256, 457
NmuCI GTSAC 1 cut(s) 550
OliI CACNNNNGTG 1 cut(s) 543
PagI TCATGA 1 cut(s) 498
PasI CCCWGGG 1 cut(s) 35
PdmI GAANNNNTTC 1 cut(s) 233
PkrI GCNGC 2 cut(s) 382, 571
PleI GAGTC 1 cut(s) 576
PpsI GAGTC 1 cut(s) 576
Psp1406I AACGTT 1 cut(s) 525
Psp6I CCWGG 1 cut(s) 34
PspGI CCWGG 1 cut(s) 34
PspN4I GGNNCC 2 cut(s) 256, 457
PstI CTGCAG 1 cut(s) 385
PstNI CAGNNNCTG 2 cut(s) 83, 377
RsaI GTAC 2 cut(s) 181, 486
RsaNI GTAC 2 cut(s) 180, 485
RseI CAYNNNNRTG 1 cut(s) 543
SatI GCNGC 2 cut(s) 381, 570
SchI GAGTC 1 cut(s) 576
ScrFI CCNGG 2 cut(s) 36, 405
SfaNI GCATC 4 cut(s) 142, 201, 208, 261
SfcI CTRYAG 1 cut(s) 381
SmiMI CAYNNNNRTG 1 cut(s) 543
Sse9I AATT 1 cut(s) 40
SsiI CCGC 2 cut(s) 8, 427
StyD4I CCNGG 2 cut(s) 34, 403
TaaI ACNGT 4 cut(s) 85, 463, 545, 594
TaiI ACGT 1 cut(s) 528
TaqI TCGA 1 cut(s) 585
TasI AATT 1 cut(s) 40
TatI WGTACW 1 cut(s) 179
TscAI CASTG 2 cut(s) 295, 468
TseFI GTSAC 1 cut(s) 550
TseI GCWGC 2 cut(s) 380, 569
Tsp45I GTSAC 1 cut(s) 550
TspDTI ATGAA 2 cut(s) 226, 515
TspRI CASTG 2 cut(s) 295, 468
XapI RAATTY 1 cut(s) 40
XmiI GTMKAC 1 cut(s) 307
XmnI GAANNNNTTC 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.