Rh7CG139000

Tim17/Tim22/Tim23/Pmp24 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0006145
Physical Location & Seq
Forward (+)
11289625 .. 11291794
2170 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG139000.1

Sequence Viewer

Length: 438 bp
ATGTTGTACGGGGGTAGCAAGGAGGCATCTGCTTCCGTTAGCAAGGATGCTGAAGTAATGTTGAAGCTTGGGAGCACACCAGACAAGCGTGAGCAATATCGTTTGATGAGAGATGCAATGGAGAAACGGTTCATTCGAGTCACGCGGGGCTCAATAGTTGGTGGAGTGCGTCTTGGATTGTTCACTGCTACCTTTTATGGTTTACAAAATCTGCTTGCTGAGAAAAGGGGTGTGCAAGATGTTTTCAATATTGTGGGAGCTGGTTCAGCCACGGCTGCGACATTCGGGCTATTATTGCCAGGATCATTACAATGGCGTGCAAGAAATATGTTACTGGGTTCGATCCTTGGAGCAGTCATTGGTTTCCCAATTGGTGATACATTCTACCTGTTGCTTCTCTTACACGTCTCAAACAATCCTATGCTGCTAACCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

145

Amino Acids

15.76

Weight (kDa)

9.98

Isoelectric Point (pI)

28.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 34 - 120 1.5e-07 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 145
AciI CCGC 1 cut(s) 145
AclWI GGATC 2 cut(s) 310, 337
AcuI CTGAAG 1 cut(s) 72
AfaI GTAC 1 cut(s) 8
AflIII ACRYGT 1 cut(s) 403
AgsI TTSAA 2 cut(s) 64, 247
AjiI CACGTC 1 cut(s) 406
AjnI CCWGG 1 cut(s) 298
AloI GAACNNNNNNTCC 2 cut(s) 113, 145
AluBI AGCT 2 cut(s) 67, 260
AluI AGCT 2 cut(s) 67, 260
Alw21I GWGCWC 1 cut(s) 77
Alw26I GTCTC 1 cut(s) 412
AlwI GGATC 2 cut(s) 310, 337
ApeKI GCWGC 2 cut(s) 275, 424
Asp700I GAANNNNTTC 1 cut(s) 128
AsuHPI GGTGA 1 cut(s) 386
BanII GRGCYC 1 cut(s) 152
Bbv12I GWGCWC 1 cut(s) 77
BbvI GCAGC 2 cut(s) 262, 411
BceAI ACGGC 1 cut(s) 288
BciT130I CCWGG 1 cut(s) 300
BcoDI GTCTC 1 cut(s) 412
BisI GCNGC 2 cut(s) 276, 425
BlsI GCNGC 2 cut(s) 277, 426
Bme1390I CCNGG 1 cut(s) 300
BmgBI CACGTC 1 cut(s) 406
BmrFI CCNGG 1 cut(s) 300
BmrI ACTGGG 1 cut(s) 344
BmsI GCATC 3 cut(s) 35, 37, 103
BmuI ACTGGG 1 cut(s) 344
BsaJI CCNNGG 2 cut(s) 270, 346
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bse1I ACTGG 1 cut(s) 339
Bse3DI GCAATG 1 cut(s) 123
BseBI CCWGG 1 cut(s) 300
BseDI CCNNGG 2 cut(s) 270, 346
BseGI GGATG 1 cut(s) 52
BseMI GCAATG 1 cut(s) 123
BseMII CTCAG 1 cut(s) 210
BseNI ACTGG 1 cut(s) 339
BseXI GCAGC 2 cut(s) 262, 411
Bsh1236I CGCG 1 cut(s) 145
BsiHKAI GWGCWC 1 cut(s) 77
BsmAI GTCTC 1 cut(s) 412
BsmBI CGTCTC 1 cut(s) 412
Bsp1286I GDGCHC 2 cut(s) 77, 152
Bsp143I GATC 2 cut(s) 302, 342
BspACI CCGC 1 cut(s) 145
BspCNI CTCAG 1 cut(s) 211
BspFNI CGCG 1 cut(s) 145
BspPI GGATC 2 cut(s) 310, 337
BsrDI GCAATG 1 cut(s) 123
BsrI ACTGG 1 cut(s) 339
BssECI CCNNGG 2 cut(s) 270, 346
BssMI GATC 2 cut(s) 302, 342
BssT1I CCWWGG 1 cut(s) 346
Bst2UI CCWGG 1 cut(s) 300
Bst4CI ACNGT 1 cut(s) 129
BstC8I GCNNGC 2 cut(s) 216, 318
BstDEI CTNAG 1 cut(s) 219
BstDSI CCRYGG 1 cut(s) 270
BstF5I GGATG 1 cut(s) 52
BstFNI CGCG 1 cut(s) 145
BstKTI GATC 2 cut(s) 305, 345
BstMAI GTCTC 1 cut(s) 412
BstMBI GATC 2 cut(s) 302, 342
BstMWI GCNNNNNNNGC 3 cut(s) 266, 275, 295
BstNI CCWGG 1 cut(s) 300
BstSCI CCNGG 1 cut(s) 298
BstUI CGCG 1 cut(s) 145
BstV1I GCAGC 2 cut(s) 262, 411
BtgI CCRYGG 1 cut(s) 270
BtrI CACGTC 1 cut(s) 406
BtsCI GGATG 1 cut(s) 52
BtsI GCAGTG 1 cut(s) 183
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 2 cut(s) 216, 318
CseI GACGC 1 cut(s) 158
Csp6I GTAC 1 cut(s) 7
CviJI RGCY 6 cut(s) 67, 150, 260, 269, 275, 289
CviKI_1 RGCY 6 cut(s) 67, 150, 260, 269, 275, 289
CviQI GTAC 1 cut(s) 7
DdeI CTNAG 1 cut(s) 219
DpnI GATC 2 cut(s) 304, 344
DpnII GATC 2 cut(s) 302, 342
Eco130I CCWWGG 1 cut(s) 346
Eco24I GRGCYC 1 cut(s) 152
Eco57I CTGAAG 1 cut(s) 72
EcoRII CCWGG 1 cut(s) 298
EcoT14I CCWWGG 1 cut(s) 346
EcoT38I GRGCYC 1 cut(s) 152
ErhI CCWWGG 1 cut(s) 346
Esp3I CGTCTC 1 cut(s) 412
FaiI YATR 3 cut(s) 198, 329, 422
FauI CCCGC 1 cut(s) 138
Fnu4HI GCNGC 2 cut(s) 276, 425
FokI GGATG 1 cut(s) 59
FriOI GRGCYC 1 cut(s) 152
Fsp4HI GCNGC 2 cut(s) 276, 425
GluI GCNGC 2 cut(s) 276, 425
HgaI GACGC 1 cut(s) 158
HindIII AAGCTT 1 cut(s) 65
HinfI GANTC 1 cut(s) 138
HphI GGTGA 1 cut(s) 386
Hpy166II GTNNAC 2 cut(s) 183, 203
Hpy8I GTNNAC 2 cut(s) 183, 203
HpyCH4III ACNGT 1 cut(s) 129
HpyCH4IV ACGT 1 cut(s) 405
HpyCH4V TGCA 3 cut(s) 116, 235, 320
HpyF10VI GCNNNNNNNGC 3 cut(s) 266, 275, 295
HpyF3I CTNAG 1 cut(s) 219
HpySE526I ACGT 1 cut(s) 405
Kzo9I GATC 2 cut(s) 302, 342
LmnI GCTCC 3 cut(s) 72, 257, 350
LpnPI CCDG 6 cut(s) 93, 246, 285, 312, 320, 401
Lsp1109I GCAGC 2 cut(s) 262, 411
LweI GCATC 3 cut(s) 35, 37, 103
MaeII ACGT 1 cut(s) 405
MaeIII GTNAC 2 cut(s) 139, 330
MalI GATC 2 cut(s) 304, 344
MboI GATC 2 cut(s) 302, 342
MfeI CAATTG 1 cut(s) 369
MhlI GDGCHC 2 cut(s) 77, 152
MluCI AATT 1 cut(s) 369
MlyI GAGTC 1 cut(s) 147
MnlI CCTC 1 cut(s) 16
MroXI GAANNNNTTC 1 cut(s) 128
MslI CAYNNNNRTG 1 cut(s) 310
MspR9I CCNGG 1 cut(s) 300
MunI CAATTG 1 cut(s) 369
MvaI CCWGG 1 cut(s) 300
MvnI CGCG 1 cut(s) 145
MwoI GCNNNNNNNGC 3 cut(s) 266, 275, 295
NdeII GATC 2 cut(s) 302, 342
NmuCI GTSAC 1 cut(s) 139
PcsI WCGNNNNNNNCGW 1 cut(s) 133
PdmI GAANNNNTTC 1 cut(s) 128
PkrI GCNGC 2 cut(s) 277, 426
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
Psp6I CCWGG 1 cut(s) 298
PspGI CCWGG 1 cut(s) 298
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
RseI CAYNNNNRTG 1 cut(s) 310
SatI GCNGC 2 cut(s) 276, 425
Sau3AI GATC 2 cut(s) 302, 342
SchI GAGTC 1 cut(s) 147
ScrFI CCNGG 1 cut(s) 300
SduI GDGCHC 2 cut(s) 77, 152
SetI ASST 6 cut(s) 69, 194, 262, 390, 408, 434
SfaNI GCATC 3 cut(s) 35, 37, 103
SmiMI CAYNNNNRTG 1 cut(s) 310
Sse9I AATT 1 cut(s) 369
SsiI CCGC 1 cut(s) 145
SspI AATATT 1 cut(s) 250
StyD4I CCNGG 1 cut(s) 298
StyI CCWWGG 1 cut(s) 346
TaaI ACNGT 1 cut(s) 129
TaiI ACGT 1 cut(s) 408
TaqI TCGA 2 cut(s) 136, 341
TasI AATT 1 cut(s) 369
TscAI CASTG 1 cut(s) 190
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 2 cut(s) 275, 424
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 1 cut(s) 121
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 1 cut(s) 190
XmnI GAANNNNTTC 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.