RchiOBHm_Chr7g0193351

Tim17/Tim22/Tim23/Pmp24 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0006145
Physical Location & Seq
Forward (+)
11696752 .. 11698907
2156 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17289

Sequence Viewer

Length: 618 bp
ATGACAATGGAATCTGGGGAGAAATCAGAAGAAACTGTATCAAAACACTCTCCGAAACTAGCTGGAACTGTCAACTGGGGAACAGCAACTGTCGCCGGAGTATTTGCAGGCATGTTGTACGGGGGTAGCAAGGAGGCATCTGCTTCGGTTAGCAAGGATGCTGAAGTAATGTTGAAGCTTGGGAGCACACCAGACAAGCGTGAGCAATATCGTTTGATGAGAGATGCAATGGAGAAACGGTTCATTCGAGTCACGCGGGGCTCAATAGTTGGTGGAGTGCGTCTTGGATTGTTCACTGCTACCTTTTATGGTTTACAAAATCTGCTTGCTGAGAAAAGGGGTGTGCAAGATGTTTTCAATATTGTGGGAGCTGGTTCAGCCACGGCTGCGACATTCGGGCTATTATTGCCAGGATCATTACAATGGCGTGCAAGAAATATGTTACTGGGTTCGATCCTTGGAGCAGTCATTGGTTTCCCAATTGGGTGGGCACACTTGAAGCTGGTGCAGATAGCAAATGAAGGGAATCCTGCTGCGCATCCTAATTCAGATCAAAGAGAAGCAAAGAGTGGTGTTGGTGCTGCCATTGAGAGGCTTGAAGAAAATTTGAATAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

205

Amino Acids

21.85

Weight (kDa)

9.55

Isoelectric Point (pI)

26.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 27 - 157 2.7e-09 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 537
AccII CGCG 1 cut(s) 256
AciI CCGC 1 cut(s) 256
AclWI GGATC 2 cut(s) 421, 448
AcsI RAATTY 1 cut(s) 604
AcuI CTGAAG 1 cut(s) 183
AfaI GTAC 1 cut(s) 119
AfiI CCNNNNNNNGG 1 cut(s) 591
AgsI TTSAA 5 cut(s) 175, 358, 499, 599, 610
AjnI CCWGG 1 cut(s) 409
AloI GAACNNNNNNTCC 2 cut(s) 224, 256
AluBI AGCT 4 cut(s) 62, 178, 371, 502
AluI AGCT 4 cut(s) 62, 178, 371, 502
Alw21I GWGCWC 1 cut(s) 188
AlwI GGATC 2 cut(s) 421, 448
AlwNI CAGNNNCTG 1 cut(s) 89
ApeKI GCWGC 3 cut(s) 386, 533, 581
ApoI RAATTY 1 cut(s) 604
Asp700I GAANNNNTTC 1 cut(s) 239
AspLEI GCGC 1 cut(s) 538
BaeGI GKGCMC 1 cut(s) 493
BanII GRGCYC 1 cut(s) 263
Bbv12I GWGCWC 1 cut(s) 188
BbvI GCAGC 3 cut(s) 373, 520, 568
BceAI ACGGC 1 cut(s) 399
BcgI CGANNNNNNTGC 2 cut(s) 126, 160
BciT130I CCWGG 1 cut(s) 411
BfaI CTAG 1 cut(s) 59
BisI GCNGC 3 cut(s) 387, 534, 582
BlsI GCNGC 3 cut(s) 388, 535, 583
Bme1390I CCNGG 1 cut(s) 411
BmrFI CCNGG 1 cut(s) 411
BmrI ACTGGG 2 cut(s) 85, 455
BmsI GCATC 4 cut(s) 146, 148, 214, 547
BmuI ACTGGG 2 cut(s) 85, 455
BsaJI CCNNGG 2 cut(s) 381, 457
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 1 cut(s) 591
Bse1I ACTGG 2 cut(s) 80, 450
Bse3DI GCAATG 1 cut(s) 234
BseBI CCWGG 1 cut(s) 411
BseDI CCNNGG 2 cut(s) 381, 457
BseGI GGATG 2 cut(s) 163, 538
BseLI CCNNNNNNNGG 1 cut(s) 591
BseMI GCAATG 1 cut(s) 234
BseMII CTCAG 1 cut(s) 321
BseNI ACTGG 2 cut(s) 80, 450
BseSI GKGCMC 1 cut(s) 493
BseXI GCAGC 3 cut(s) 373, 520, 568
BsgI GTGCAG 1 cut(s) 527
Bsh1236I CGCG 1 cut(s) 256
BsiHKAI GWGCWC 1 cut(s) 188
BsiSI CCGG 1 cut(s) 96
BslI CCNNNNNNNGG 1 cut(s) 591
Bsp1286I GDGCHC 3 cut(s) 188, 263, 493
Bsp143I GATC 3 cut(s) 413, 453, 550
BspACI CCGC 1 cut(s) 256
BspCNI CTCAG 1 cut(s) 322
BspFNI CGCG 1 cut(s) 256
BspPI GGATC 2 cut(s) 421, 448
BsrDI GCAATG 1 cut(s) 234
BsrI ACTGG 2 cut(s) 80, 450
BssECI CCNNGG 2 cut(s) 381, 457
BssMI GATC 3 cut(s) 413, 453, 550
BssT1I CCWWGG 1 cut(s) 457
Bst2UI CCWGG 1 cut(s) 411
Bst4CI ACNGT 4 cut(s) 37, 70, 91, 240
BstC8I GCNNGC 3 cut(s) 109, 327, 429
BstDEI CTNAG 1 cut(s) 330
BstDSI CCRYGG 1 cut(s) 381
BstF5I GGATG 2 cut(s) 163, 538
BstFNI CGCG 1 cut(s) 256
BstHHI GCGC 1 cut(s) 538
BstKTI GATC 3 cut(s) 416, 456, 553
BstMBI GATC 3 cut(s) 413, 453, 550
BstMWI GCNNNNNNNGC 4 cut(s) 92, 377, 386, 406
BstNI CCWGG 1 cut(s) 411
BstNSI RCATGY 1 cut(s) 115
BstSCI CCNGG 1 cut(s) 409
BstSLI GKGCMC 1 cut(s) 493
BstUI CGCG 1 cut(s) 256
BstV1I GCAGC 3 cut(s) 373, 520, 568
BstXI CCANNNNNNTGG 1 cut(s) 486
BtgI CCRYGG 1 cut(s) 381
BtsCI GGATG 2 cut(s) 163, 538
BtsI GCAGTG 1 cut(s) 294
BtsIMutI CAGTG 1 cut(s) 294
Cac8I GCNNGC 3 cut(s) 109, 327, 429
CaiI CAGNNNCTG 1 cut(s) 89
CfoI GCGC 1 cut(s) 538
CseI GACGC 1 cut(s) 269
Csp6I GTAC 1 cut(s) 118
CviAII CATG 1 cut(s) 112
CviJI RGCY 9 cut(s) 62, 178, 261, 371, 380, 386, 400, 502, 595
CviKI_1 RGCY 9 cut(s) 62, 178, 261, 371, 380, 386, 400, 502, 595
CviQI GTAC 1 cut(s) 118
DdeI CTNAG 1 cut(s) 330
DpnI GATC 3 cut(s) 415, 455, 552
DpnII GATC 3 cut(s) 413, 453, 550
Eco130I CCWWGG 1 cut(s) 457
Eco24I GRGCYC 1 cut(s) 263
Eco57I CTGAAG 1 cut(s) 183
EcoRII CCWGG 1 cut(s) 409
EcoT14I CCWWGG 1 cut(s) 457
EcoT38I GRGCYC 1 cut(s) 263
ErhI CCWWGG 1 cut(s) 457
FaeI CATG 1 cut(s) 115
FaiI YATR 3 cut(s) 113, 309, 440
FatI CATG 1 cut(s) 111
FauI CCCGC 1 cut(s) 249
Fnu4HI GCNGC 3 cut(s) 387, 534, 582
FokI GGATG 2 cut(s) 170, 525
FriOI GRGCYC 1 cut(s) 263
Fsp4HI GCNGC 3 cut(s) 387, 534, 582
FspBI CTAG 1 cut(s) 59
FspI TGCGCA 1 cut(s) 537
GlaI GCGC 1 cut(s) 537
GluI GCNGC 3 cut(s) 387, 534, 582
HapII CCGG 1 cut(s) 96
HgaI GACGC 1 cut(s) 269
HhaI GCGC 1 cut(s) 538
Hin1II CATG 1 cut(s) 115
Hin6I GCGC 1 cut(s) 536
HinP1I GCGC 1 cut(s) 536
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HindIII AAGCTT 1 cut(s) 176
HinfI GANTC 3 cut(s) 11, 249, 526
HpaII CCGG 1 cut(s) 96
Hpy166II GTNNAC 3 cut(s) 73, 294, 314
Hpy188I TCNGA 3 cut(s) 28, 54, 550
Hpy8I GTNNAC 3 cut(s) 73, 294, 314
HpyAV CCTTC 1 cut(s) 515
HpyCH4III ACNGT 4 cut(s) 37, 70, 91, 240
HpyCH4V TGCA 5 cut(s) 107, 227, 346, 431, 508
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 377, 386, 406
HpyF3I CTNAG 1 cut(s) 330
Hsp92II CATG 1 cut(s) 115
HspAI GCGC 1 cut(s) 536
Kzo9I GATC 3 cut(s) 413, 453, 550
LmnI GCTCC 3 cut(s) 183, 368, 461
Lsp1109I GCAGC 3 cut(s) 373, 520, 568
LweI GCATC 4 cut(s) 146, 148, 214, 547
MaeI CTAG 1 cut(s) 59
MaeIII GTNAC 2 cut(s) 250, 441
MalI GATC 3 cut(s) 415, 455, 552
MboI GATC 3 cut(s) 413, 453, 550
MboII GAAGA 2 cut(s) 41, 611
MfeI CAATTG 1 cut(s) 480
MhlI GDGCHC 3 cut(s) 188, 263, 493
MluCI AATT 3 cut(s) 480, 544, 604
MlyI GAGTC 1 cut(s) 258
MnlI CCTC 2 cut(s) 127, 585
MroXI GAANNNNTTC 1 cut(s) 239
MslI CAYNNNNRTG 1 cut(s) 421
MspI CCGG 1 cut(s) 96
MspR9I CCNGG 1 cut(s) 411
MunI CAATTG 1 cut(s) 480
MvaI CCWGG 1 cut(s) 411
MvnI CGCG 1 cut(s) 256
MwoI GCNNNNNNNGC 4 cut(s) 92, 377, 386, 406
NdeII GATC 3 cut(s) 413, 453, 550
NlaIII CATG 1 cut(s) 115
NmuCI GTSAC 1 cut(s) 250
NsbI TGCGCA 1 cut(s) 537
NspI RCATGY 1 cut(s) 115
PcsI WCGNNNNNNNCGW 1 cut(s) 244
PdmI GAANNNNTTC 1 cut(s) 239
PfeI GAWTC 2 cut(s) 11, 526
PkrI GCNGC 3 cut(s) 388, 535, 583
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
Psp6I CCWGG 1 cut(s) 409
PspGI CCWGG 1 cut(s) 409
PstNI CAGNNNCTG 1 cut(s) 89
RsaI GTAC 1 cut(s) 119
RsaNI GTAC 1 cut(s) 118
RseI CAYNNNNRTG 1 cut(s) 421
SatI GCNGC 3 cut(s) 387, 534, 582
Sau3AI GATC 3 cut(s) 413, 453, 550
SchI GAGTC 1 cut(s) 258
ScrFI CCNGG 1 cut(s) 411
SduI GDGCHC 3 cut(s) 188, 263, 493
SetI ASST 5 cut(s) 64, 180, 305, 373, 504
SfaNI GCATC 4 cut(s) 146, 148, 214, 547
SmiMI CAYNNNNRTG 1 cut(s) 421
Sse9I AATT 3 cut(s) 480, 544, 604
SsiI CCGC 1 cut(s) 256
SspI AATATT 1 cut(s) 361
SspMI CTAG 1 cut(s) 59
StyD4I CCNGG 1 cut(s) 409
StyI CCWWGG 1 cut(s) 457
TaaI ACNGT 4 cut(s) 37, 70, 91, 240
TaqI TCGA 2 cut(s) 247, 452
TasI AATT 3 cut(s) 480, 544, 604
TfiI GAWTC 2 cut(s) 11, 526
TscAI CASTG 1 cut(s) 301
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 3 cut(s) 386, 533, 581
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 2 cut(s) 232, 534
TspRI CASTG 1 cut(s) 301
XapI RAATTY 1 cut(s) 604
XceI RCATGY 1 cut(s) 115
XmnI GAANNNNTTC 1 cut(s) 239
XspI CTAG 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.