Rroxscaffold_3G00262190

Tim17/Tim22/Tim23/Pmp24 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006145
Physical Location & Seq
Reverse (-)
56258696 .. 56260672
1977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00262190.1

Sequence Viewer

Length: 726 bp
ATGAGAAGGGGCGATGGGCCGCTCCAACAACAAAGAGCACTAGTTTTCTTTGGAACAAACACGACATTATCAAGGACGTCGTCTTTGGGTGGTTCGCGTGCAGAGCCCATATACTACTATATGACAATGGAAGCTGGGGAGAAATCAGAAGAAACTCATTCTCCGAAACTAGCTGGAACTGTCAACTGGGGAACGGCAACTGTCGCCGGAGTATTTGCAGGCATGTTGTACGGGGTAGCAAGGAGGCATCTGCTTCGGTTCAAGGATGCTGAAGTAATGTTGAAGCTTGGGAGCACACCAGACAAGCGTGAGCAATATCGTTTGATGAGAGATGCAATGGAGAAACGGTTCATTCGAGTCACGCGGGGTTCAATAGTTGGTGGAGTGCGTCTTGGATTGTTCACTGCTACCTTTTATGGTTTACAAAATCTGCTTGCTGAGAAAAGGGGTGTGCAAGATGTTTTCAATATTGTGGGAGCTGGTTCAGCCACGGCTGCGACATTCGGGCTATTATTGCCAGGATCATTACAATGGCGTGCAAGAAATATGTTACTGGGTTCGATCCTTGGAGCAGTCATTGGTTTCCCAATTGGGTGGTCACACTTGAAGCTGGTGCAGATAGCAAATGAAGGGAATCCTGCTGCGCATCCTAATTCAGATCAAAGAGAAGCAAAGAGTGGTGTTGGTGCTGCCATTGAGAGGCTTGAAGAAAATTTGAATAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

241

Amino Acids

26.26

Weight (kDa)

10.08

Isoelectric Point (pI)

37.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 64 - 193 9.2e-09 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 80
Acc16I TGCGCA 1 cut(s) 645
AccBSI CCGCTC 1 cut(s) 22
AccII CGCG 2 cut(s) 97, 364
AciI CCGC 2 cut(s) 20, 364
AclWI GGATC 2 cut(s) 529, 556
AcsI RAATTY 1 cut(s) 712
AcuI CTGAAG 1 cut(s) 291
AcyI GRCGYC 1 cut(s) 77
AfaI GTAC 1 cut(s) 230
AfiI CCNNNNNNNGG 1 cut(s) 699
AgsI TTSAA 7 cut(s) 262, 283, 372, 466, 607, 707, 718
AhlI ACTAGT 1 cut(s) 40
AjnI CCWGG 1 cut(s) 517
AloI GAACNNNNNNTCC 2 cut(s) 332, 364
AluBI AGCT 5 cut(s) 134, 173, 286, 479, 610
AluI AGCT 5 cut(s) 134, 173, 286, 479, 610
Alw21I GWGCWC 2 cut(s) 40, 296
AlwI GGATC 2 cut(s) 529, 556
AoxI GGCC 1 cut(s) 17
ApeKI GCWGC 3 cut(s) 494, 641, 689
ApoI RAATTY 1 cut(s) 712
ArsI GACNNNNNNTTYG 2 cut(s) 67, 99
Asp700I GAANNNNTTC 1 cut(s) 347
AspLEI GCGC 1 cut(s) 646
AspS9I GGNCC 1 cut(s) 17
BanII GRGCYC 1 cut(s) 108
Bbv12I GWGCWC 2 cut(s) 40, 296
BbvI GCAGC 3 cut(s) 481, 628, 676
BccI CCATC 1 cut(s) 8
BceAI ACGGC 2 cut(s) 210, 507
BcgI CGANNNNNNTGC 2 cut(s) 236, 270
BciT130I CCWGG 1 cut(s) 519
BcuI ACTAGT 1 cut(s) 40
BfaI CTAG 2 cut(s) 41, 170
BisI GCNGC 4 cut(s) 20, 495, 642, 690
BlsI GCNGC 4 cut(s) 21, 496, 643, 691
Bme1390I CCNGG 1 cut(s) 519
BmgT120I GGNCC 1 cut(s) 17
BmrFI CCNGG 1 cut(s) 519
BmrI ACTGGG 2 cut(s) 196, 563
BmsI GCATC 4 cut(s) 256, 256, 322, 655
BmuI ACTGGG 2 cut(s) 196, 563
BsaHI GRCGYC 1 cut(s) 77
BsaJI CCNNGG 2 cut(s) 489, 565
BsaXI ACNNNNNCTCC 4 cut(s) 145, 175, 332, 362
Bsc4I CCNNNNNNNGG 1 cut(s) 699
Bse1I ACTGG 2 cut(s) 191, 558
Bse3DI GCAATG 1 cut(s) 342
BseBI CCWGG 1 cut(s) 519
BseDI CCNNGG 2 cut(s) 489, 565
BseGI GGATG 2 cut(s) 271, 646
BseLI CCNNNNNNNGG 1 cut(s) 699
BseMI GCAATG 1 cut(s) 342
BseMII CTCAG 1 cut(s) 429
BseNI ACTGG 2 cut(s) 191, 558
BseXI GCAGC 3 cut(s) 481, 628, 676
BseYI CCCAGC 1 cut(s) 134
BsgI GTGCAG 2 cut(s) 120, 635
Bsh1236I CGCG 2 cut(s) 97, 364
BshFI GGCC 1 cut(s) 19
BsiHKAI GWGCWC 2 cut(s) 40, 296
BsiSI CCGG 1 cut(s) 207
BslI CCNNNNNNNGG 1 cut(s) 699
BsnI GGCC 1 cut(s) 19
Bsp1286I GDGCHC 3 cut(s) 40, 108, 296
Bsp143I GATC 3 cut(s) 521, 561, 658
BspACI CCGC 2 cut(s) 20, 364
BspANI GGCC 1 cut(s) 19
BspCNI CTCAG 1 cut(s) 430
BspFNI CGCG 2 cut(s) 97, 364
BspPI GGATC 2 cut(s) 529, 556
BsrBI CCGCTC 1 cut(s) 22
BsrDI GCAATG 1 cut(s) 342
BsrI ACTGG 2 cut(s) 191, 558
BssECI CCNNGG 2 cut(s) 489, 565
BssMI GATC 3 cut(s) 521, 561, 658
BssNI GRCGYC 1 cut(s) 77
BssT1I CCWWGG 1 cut(s) 565
Bst2UI CCWGG 1 cut(s) 519
Bst4CI ACNGT 3 cut(s) 181, 202, 348
BstACI GRCGYC 1 cut(s) 77
BstC8I GCNNGC 4 cut(s) 99, 220, 435, 537
BstDEI CTNAG 1 cut(s) 438
BstDSI CCRYGG 1 cut(s) 489
BstF5I GGATG 2 cut(s) 271, 646
BstFNI CGCG 2 cut(s) 97, 364
BstHHI GCGC 1 cut(s) 646
BstKTI GATC 3 cut(s) 524, 564, 661
BstMBI GATC 3 cut(s) 521, 561, 658
BstMWI GCNNNNNNNGC 5 cut(s) 103, 203, 485, 494, 514
BstNI CCWGG 1 cut(s) 519
BstNSI RCATGY 1 cut(s) 226
BstSCI CCNGG 1 cut(s) 517
BstUI CGCG 2 cut(s) 97, 364
BstV1I GCAGC 3 cut(s) 481, 628, 676
BstXI CCANNNNNNTGG 1 cut(s) 594
BsuRI GGCC 1 cut(s) 19
BtgI CCRYGG 1 cut(s) 489
BtgZI GCGATG 1 cut(s) 27
BtsCI GGATG 2 cut(s) 271, 646
BtsI GCAGTG 1 cut(s) 402
BtsIMutI CAGTG 1 cut(s) 402
Cac8I GCNNGC 4 cut(s) 99, 220, 435, 537
CfoI GCGC 1 cut(s) 646
Cfr13I GGNCC 1 cut(s) 17
CseI GACGC 1 cut(s) 377
Csp6I GTAC 1 cut(s) 229
CviAII CATG 1 cut(s) 223
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 1 cut(s) 438
DpnI GATC 3 cut(s) 523, 563, 660
DpnII GATC 3 cut(s) 521, 561, 658
Eco130I CCWWGG 1 cut(s) 565
Eco24I GRGCYC 1 cut(s) 108
Eco57I CTGAAG 1 cut(s) 291
EcoRII CCWGG 1 cut(s) 517
EcoT14I CCWWGG 1 cut(s) 565
EcoT38I GRGCYC 1 cut(s) 108
ErhI CCWWGG 1 cut(s) 565
FaeI CATG 1 cut(s) 226
FaiI YATR 7 cut(s) 110, 112, 120, 122, 224, 417, 548
FatI CATG 1 cut(s) 222
FauI CCCGC 1 cut(s) 357
Fnu4HI GCNGC 4 cut(s) 20, 495, 642, 690
FokI GGATG 2 cut(s) 278, 633
FriOI GRGCYC 1 cut(s) 108
Fsp4HI GCNGC 4 cut(s) 20, 495, 642, 690
FspBI CTAG 2 cut(s) 41, 170
FspI TGCGCA 1 cut(s) 645
GlaI GCGC 1 cut(s) 645
GluI GCNGC 4 cut(s) 20, 495, 642, 690
GsaI CCCAGC 1 cut(s) 138
HaeIII GGCC 1 cut(s) 19
HapII CCGG 1 cut(s) 207
HgaI GACGC 1 cut(s) 377
HhaI GCGC 1 cut(s) 646
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 1 cut(s) 226
Hin6I GCGC 1 cut(s) 644
HinP1I GCGC 1 cut(s) 644
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 2 cut(s) 357, 634
HpaII CCGG 1 cut(s) 207
Hpy166II GTNNAC 3 cut(s) 184, 402, 422
Hpy188I TCNGA 3 cut(s) 148, 165, 658
Hpy8I GTNNAC 3 cut(s) 184, 402, 422
Hpy99I CGWCG 1 cut(s) 82
HpyAV CCTTC 1 cut(s) 623
HpyCH4III ACNGT 3 cut(s) 181, 202, 348
HpyCH4IV ACGT 1 cut(s) 77
HpyCH4V TGCA 6 cut(s) 101, 218, 335, 454, 539, 616
HpyF10VI GCNNNNNNNGC 5 cut(s) 103, 203, 485, 494, 514
HpyF3I CTNAG 1 cut(s) 438
HpySE526I ACGT 1 cut(s) 77
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 1 cut(s) 226
HspAI GCGC 1 cut(s) 644
Kzo9I GATC 3 cut(s) 521, 561, 658
LmnI GCTCC 4 cut(s) 27, 291, 476, 569
Lsp1109I GCAGC 3 cut(s) 481, 628, 676
LweI GCATC 4 cut(s) 256, 256, 322, 655
MaeI CTAG 2 cut(s) 41, 170
MaeII ACGT 1 cut(s) 77
MaeIII GTNAC 3 cut(s) 358, 549, 597
MalI GATC 3 cut(s) 523, 563, 660
MbiI CCGCTC 1 cut(s) 22
MboI GATC 3 cut(s) 521, 561, 658
MboII GAAGA 2 cut(s) 161, 719
MfeI CAATTG 1 cut(s) 588
MhlI GDGCHC 3 cut(s) 40, 108, 296
MluCI AATT 3 cut(s) 588, 652, 712
MlyI GAGTC 1 cut(s) 366
MmeI TCCRAC 1 cut(s) 49
MnlI CCTC 2 cut(s) 237, 693
MroXI GAANNNNTTC 1 cut(s) 347
MslI CAYNNNNRTG 1 cut(s) 529
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 1 cut(s) 519
MunI CAATTG 1 cut(s) 588
MvaI CCWGG 1 cut(s) 519
MvnI CGCG 2 cut(s) 97, 364
MwoI GCNNNNNNNGC 5 cut(s) 103, 203, 485, 494, 514
NdeII GATC 3 cut(s) 521, 561, 658
NlaIII CATG 1 cut(s) 226
NmuCI GTSAC 2 cut(s) 358, 597
NsbI TGCGCA 1 cut(s) 645
NspI RCATGY 1 cut(s) 226
PcsI WCGNNNNNNNCGW 1 cut(s) 352
PdmI GAANNNNTTC 1 cut(s) 347
PfeI GAWTC 1 cut(s) 634
PflFI GACNNNGTC 1 cut(s) 79
PkrI GCNGC 4 cut(s) 21, 496, 643, 691
PleI GAGTC 1 cut(s) 365
PpsI GAGTC 1 cut(s) 365
Psp6I CCWGG 1 cut(s) 517
PspFI CCCAGC 1 cut(s) 134
PspGI CCWGG 1 cut(s) 517
PspPI GGNCC 1 cut(s) 17
PsyI GACNNNGTC 1 cut(s) 79
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
RseI CAYNNNNRTG 1 cut(s) 529
SatI GCNGC 4 cut(s) 20, 495, 642, 690
Sau3AI GATC 3 cut(s) 521, 561, 658
Sau96I GGNCC 1 cut(s) 17
SchI GAGTC 1 cut(s) 366
ScrFI CCNGG 1 cut(s) 519
SduI GDGCHC 3 cut(s) 40, 108, 296
SetI ASST 7 cut(s) 80, 136, 175, 288, 413, 481, 612
SfaNI GCATC 4 cut(s) 256, 256, 322, 655
SmiMI CAYNNNNRTG 1 cut(s) 529
SpeI ACTAGT 1 cut(s) 40
Sse9I AATT 3 cut(s) 588, 652, 712
SsiI CCGC 2 cut(s) 20, 364
SspI AATATT 1 cut(s) 469
SspMI CTAG 2 cut(s) 41, 170
StyD4I CCNGG 1 cut(s) 517
StyI CCWWGG 1 cut(s) 565
TaaI ACNGT 3 cut(s) 181, 202, 348
TaiI ACGT 1 cut(s) 80
TaqI TCGA 2 cut(s) 355, 560
TasI AATT 3 cut(s) 588, 652, 712
TauI GCSGC 1 cut(s) 22
TfiI GAWTC 1 cut(s) 634
TscAI CASTG 1 cut(s) 409
TseFI GTSAC 2 cut(s) 358, 597
TseI GCWGC 3 cut(s) 494, 641, 689
Tsp45I GTSAC 2 cut(s) 358, 597
TspDTI ATGAA 2 cut(s) 340, 642
TspRI CASTG 1 cut(s) 409
Tth111I GACNNNGTC 1 cut(s) 79
XapI RAATTY 1 cut(s) 712
XceI RCATGY 1 cut(s) 226
XmnI GAANNNNTTC 1 cut(s) 347
XspI CTAG 2 cut(s) 41, 170
ZraI GACGTC 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.