FvH4_5g05480

Tim17/Tim22/Tim23/Pmp24 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006145
Physical Location & Seq
Reverse (-)
3225656 .. 3227477
1822 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g05480.t1

Sequence Viewer

Length: 600 bp
ATGGAAGCTCAGGAGAAACCCGAAGAAACTGTATCCCAACATTCTCCGAAACTAGCTGGGACTGTCAACTGGGGGACAGCAACTGTTGCCGGAGTGTTTGCAGGCATGTTGTATGGTGGTAGCAAGGAGGCATCTGCTTCCGTTAGCAAGGATGCTGAAGTTATGTTGAAGCTTGGGAGCACACCAGACAAGCGTGAGCAATATCGTATGATGAGAGATGCAATGGAGAAAAGGTTCATCCGAGTCACGCGGGGTTCAATAATTGGCGGAGTGCGTCTTGGGTTGTTCACTGCTACCTTTTATGGTTTGCAGAATCTGCTTGCTGAGAAAAGGGCTGTGCTAGATGTTTTCAATATTGTGGGAGCTGGTTCTGCCACTGCTGCGACATTCGGGCTATTATTGCCAGGATCATTACAATGGCGTGGAAGAAATATGTTGCTGGGGTCAATCCTTGGAGGAGCCATTGGTTTCCCACTTGGGTGGGCACACTTGAAGCTGGTGGAGATGGCAAATGAAGGGAATCCCACCTTGCATCAAAGAGAACCACAGAGTGGTGTTGGTGCTGCTATTGAGAGGCTTGAAGAAAATTTGAATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

200

Amino Acids

21.36

Weight (kDa)

9.26

Isoelectric Point (pI)

33.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 25 - 155 3e-07 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 551
AccII CGCG 1 cut(s) 250
AciI CCGC 2 cut(s) 250, 267
AclWI GGATC 1 cut(s) 415
AcsI RAATTY 1 cut(s) 586
AcuI CTGAAG 1 cut(s) 177
AdeI CACNNNGTG 1 cut(s) 551
AfiI CCNNNNNNNGG 1 cut(s) 551
AgsI TTSAA 6 cut(s) 169, 258, 352, 493, 581, 592
AjnI CCWGG 1 cut(s) 403
AleI CACNNNNGTG 1 cut(s) 478
AloI GAACNNNNNNTCC 2 cut(s) 218, 250
AluBI AGCT 5 cut(s) 8, 56, 172, 365, 496
AluI AGCT 5 cut(s) 8, 56, 172, 365, 496
Alw21I GWGCWC 1 cut(s) 182
AlwI GGATC 1 cut(s) 415
AlwNI CAGNNNCTG 2 cut(s) 83, 316
ApeKI GCWGC 2 cut(s) 380, 563
ApoI RAATTY 1 cut(s) 586
Asp700I GAANNNNTTC 1 cut(s) 233
BaeGI GKGCMC 1 cut(s) 487
Bbv12I GWGCWC 1 cut(s) 182
BbvI GCAGC 2 cut(s) 367, 550
BccI CCATC 1 cut(s) 499
BciT130I CCWGG 1 cut(s) 405
BciVI GTATCC 1 cut(s) 43
BfaI CTAG 2 cut(s) 53, 341
BfuI GTATCC 1 cut(s) 43
BisI GCNGC 2 cut(s) 381, 564
BlsI GCNGC 2 cut(s) 382, 565
Bme1390I CCNGG 1 cut(s) 405
BmiI GGNNCC 1 cut(s) 460
BmrFI CCNGG 1 cut(s) 405
BmrI ACTGGG 1 cut(s) 79
BmsI GCATC 4 cut(s) 140, 142, 208, 541
BmuI ACTGGG 1 cut(s) 79
Bpu10I CCTNAGC 1 cut(s) 9
BsaJI CCNNGG 1 cut(s) 451
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 1 cut(s) 551
Bse1I ACTGG 1 cut(s) 74
Bse3DI GCAATG 1 cut(s) 228
BseBI CCWGG 1 cut(s) 405
BseDI CCNNGG 1 cut(s) 451
BseGI GGATG 2 cut(s) 157, 237
BseLI CCNNNNNNNGG 1 cut(s) 551
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 2 cut(s) 23, 315
BseNI ACTGG 1 cut(s) 74
BseRI GAGGAG 1 cut(s) 471
BseSI GKGCMC 1 cut(s) 487
BseXI GCAGC 2 cut(s) 367, 550
BseYI CCCAGC 2 cut(s) 56, 439
Bsh1236I CGCG 1 cut(s) 250
BsiHKAI GWGCWC 1 cut(s) 182
BsiSI CCGG 1 cut(s) 90
BslFI GGGAC 2 cut(s) 73, 88
BslI CCNNNNNNNGG 1 cut(s) 551
BsmFI GGGAC 2 cut(s) 73, 88
Bsp1286I GDGCHC 2 cut(s) 182, 487
Bsp143I GATC 1 cut(s) 407
BspACI CCGC 2 cut(s) 250, 267
BspCNI CTCAG 2 cut(s) 22, 316
BspFNI CGCG 1 cut(s) 250
BspLI GGNNCC 1 cut(s) 460
BspPI GGATC 1 cut(s) 415
BsrDI GCAATG 1 cut(s) 228
BsrI ACTGG 1 cut(s) 74
BssECI CCNNGG 1 cut(s) 451
BssMI GATC 1 cut(s) 407
BssT1I CCWWGG 1 cut(s) 451
Bst2UI CCWGG 1 cut(s) 405
Bst4CI ACNGT 3 cut(s) 31, 64, 85
BstAPI GCANNNNNTGC 2 cut(s) 86, 316
BstC8I GCNNGC 2 cut(s) 103, 321
BstDEI CTNAG 2 cut(s) 9, 324
BstF5I GGATG 2 cut(s) 157, 237
BstFNI CGCG 1 cut(s) 250
BstKTI GATC 1 cut(s) 410
BstMBI GATC 1 cut(s) 407
BstMWI GCNNNNNNNGC 5 cut(s) 86, 316, 371, 380, 400
BstNI CCWGG 1 cut(s) 405
BstNSI RCATGY 1 cut(s) 109
BstSCI CCNGG 1 cut(s) 403
BstSLI GKGCMC 1 cut(s) 487
BstUI CGCG 1 cut(s) 250
BstV1I GCAGC 2 cut(s) 367, 550
BstXI CCANNNNNNTGG 1 cut(s) 480
BsuI GTATCC 1 cut(s) 43
BtsCI GGATG 2 cut(s) 157, 237
BtsI GCAGTG 2 cut(s) 288, 375
BtsIMutI CAGTG 2 cut(s) 288, 375
Cac8I GCNNGC 2 cut(s) 103, 321
CaiI CAGNNNCTG 2 cut(s) 83, 316
CseI GACGC 1 cut(s) 263
CviAII CATG 1 cut(s) 106
CviJI RGCY 9 cut(s) 8, 56, 172, 335, 365, 394, 461, 496, 577
CviKI_1 RGCY 9 cut(s) 8, 56, 172, 335, 365, 394, 461, 496, 577
DdeI CTNAG 2 cut(s) 9, 324
DpnI GATC 1 cut(s) 409
DpnII GATC 1 cut(s) 407
DraIII CACNNNGTG 1 cut(s) 551
EciI GGCGGA 1 cut(s) 282
Eco130I CCWWGG 1 cut(s) 451
Eco57I CTGAAG 1 cut(s) 177
EcoRII CCWGG 1 cut(s) 403
EcoT14I CCWWGG 1 cut(s) 451
ErhI CCWWGG 1 cut(s) 451
FaeI CATG 1 cut(s) 109
FaiI YATR 6 cut(s) 107, 114, 164, 209, 303, 434
FaqI GGGAC 2 cut(s) 73, 88
FatI CATG 1 cut(s) 105
FauI CCCGC 1 cut(s) 243
Fnu4HI GCNGC 2 cut(s) 381, 564
FokI GGATG 2 cut(s) 164, 224
Fsp4HI GCNGC 2 cut(s) 381, 564
FspBI CTAG 2 cut(s) 53, 341
GluI GCNGC 2 cut(s) 381, 564
GsaI CCCAGC 2 cut(s) 60, 443
HapII CCGG 1 cut(s) 90
HgaI GACGC 1 cut(s) 263
Hin1II CATG 1 cut(s) 109
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 3 cut(s) 243, 313, 520
HpaII CCGG 1 cut(s) 90
Hpy166II GTNNAC 2 cut(s) 67, 288
Hpy188I TCNGA 2 cut(s) 48, 242
Hpy188III TCNNGA 1 cut(s) 11
Hpy8I GTNNAC 2 cut(s) 67, 288
HpyAV CCTTC 1 cut(s) 509
HpyCH4III ACNGT 3 cut(s) 31, 64, 85
HpyCH4V TGCA 4 cut(s) 101, 221, 310, 532
HpyF10VI GCNNNNNNNGC 5 cut(s) 86, 316, 371, 380, 400
HpyF3I CTNAG 2 cut(s) 9, 324
Hsp92II CATG 1 cut(s) 109
Kzo9I GATC 1 cut(s) 407
LmnI GCTCC 3 cut(s) 177, 362, 458
Lsp1109I GCAGC 2 cut(s) 367, 550
LweI GCATC 4 cut(s) 140, 142, 208, 541
MaeI CTAG 2 cut(s) 53, 341
MaeIII GTNAC 1 cut(s) 244
MalI GATC 1 cut(s) 409
MboI GATC 1 cut(s) 407
MboII GAAGA 3 cut(s) 35, 438, 593
MhlI GDGCHC 2 cut(s) 182, 487
MluCI AATT 2 cut(s) 261, 586
MlyI GAGTC 1 cut(s) 252
MnlI CCTC 3 cut(s) 121, 449, 567
MroXI GAANNNNTTC 1 cut(s) 233
MslI CAYNNNNRTG 2 cut(s) 415, 478
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 1 cut(s) 405
MvaI CCWGG 1 cut(s) 405
MvnI CGCG 1 cut(s) 250
MwoI GCNNNNNNNGC 5 cut(s) 86, 316, 371, 380, 400
NdeII GATC 1 cut(s) 407
NlaIII CATG 1 cut(s) 109
NlaIV GGNNCC 1 cut(s) 460
NmuCI GTSAC 1 cut(s) 244
NspI RCATGY 1 cut(s) 109
OliI CACNNNNGTG 1 cut(s) 478
PdmI GAANNNNTTC 1 cut(s) 233
PfeI GAWTC 2 cut(s) 313, 520
PflMI CCANNNNNTGG 1 cut(s) 551
PkrI GCNGC 2 cut(s) 382, 565
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
Psp6I CCWGG 1 cut(s) 403
PspFI CCCAGC 2 cut(s) 56, 439
PspGI CCWGG 1 cut(s) 403
PspN4I GGNNCC 1 cut(s) 460
PstNI CAGNNNCTG 2 cut(s) 83, 316
RseI CAYNNNNRTG 2 cut(s) 415, 478
SatI GCNGC 2 cut(s) 381, 564
Sau3AI GATC 1 cut(s) 407
SchI GAGTC 1 cut(s) 252
ScrFI CCNGG 1 cut(s) 405
SduI GDGCHC 2 cut(s) 182, 487
SetI ASST 8 cut(s) 10, 58, 174, 236, 299, 367, 498, 530
SfaNI GCATC 4 cut(s) 140, 142, 208, 541
SmiMI CAYNNNNRTG 2 cut(s) 415, 478
Sse9I AATT 2 cut(s) 261, 586
SsiI CCGC 2 cut(s) 250, 267
SspI AATATT 1 cut(s) 355
SspMI CTAG 2 cut(s) 53, 341
StyD4I CCNGG 1 cut(s) 403
StyI CCWWGG 1 cut(s) 451
TaaI ACNGT 3 cut(s) 31, 64, 85
TasI AATT 2 cut(s) 261, 586
TfiI GAWTC 2 cut(s) 313, 520
TscAI CASTG 2 cut(s) 295, 382
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 2 cut(s) 380, 563
Tsp45I GTSAC 1 cut(s) 244
TspDTI ATGAA 2 cut(s) 226, 528
TspGWI ACGGA 1 cut(s) 130
TspRI CASTG 2 cut(s) 295, 382
Van91I CCANNNNNTGG 1 cut(s) 551
XapI RAATTY 1 cut(s) 586
XceI RCATGY 1 cut(s) 109
XmnI GAANNNNTTC 1 cut(s) 233
XspI CTAG 2 cut(s) 53, 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.