MD14G1178100.v1.1

Tim17/Tim22/Tim23/Pmp24 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006145
Physical Location & Seq
Forward (+)
27088831 .. 27090410
1580 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1178100.v1.1.491

Sequence Viewer

Length: 603 bp
ATGGAAGCTCCCGAAGACCGCGTCTCAAACCATTCTCCGAAACTAGCAGGAACTGTCAACTGGGGCACGGCGACTGTCATCGGGGTGTTTGCAGGCTTGTTTTACGGTGGTAGCAAGGAAGCAGCTGCTTCAGTTAGCAAAGATGCAGAAGTAATGTTGAAGCTGGGGAGCACGCCAGACAAGCGTGAACAATATCGTCTCATGAGAGATGCAATGGAGAAGAGGTTCATTCGTGTCACTCGGGGCTCGATAGTTGGTGGAGTGCGTCTTGGATTGTTTACTGCAACTTTTTGTGGTATGCAGAATCTGCTTGCCGAGAAAAGGGGCGTGCATGATGTTTTCAATGTTGTTGGAGCCGGTTCAGCCACTGCTGCTACGTTTGCTCTAATAATGCCAGGATCGTTGGCTTGGCGAGCAAGAAACGTGATGTTGGGTACTGCCCTAGGGGCGGCGTTTGGTTTTCCACTTGGGTGGGCCCATTTGAAGCTGGTAGGGAAGGCAAATGAAGGGAATCTGGCTGCACATCCTGATTCAGATCAAAGAGAAGCAAGGAGTGGTGTTGGTGCTGCAATTGACAGGCTGGAAGAAAACTTGAATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

201

Amino Acids

21.23

Weight (kDa)

9.56

Isoelectric Point (pI)

19.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 22 - 152 3.7e-09 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 21
AciI CCGC 2 cut(s) 19, 449
AclWI GGATC 1 cut(s) 406
AcuI CTGAAG 1 cut(s) 114
AfaI GTAC 1 cut(s) 436
AfiI CCNNNNNNNGG 2 cut(s) 321, 448
AgsI TTSAA 4 cut(s) 160, 343, 484, 595
AjnI CCWGG 1 cut(s) 394
AleI CACNNNNGTG 1 cut(s) 469
AloI GAACNNNNNNTCC 2 cut(s) 209, 241
AluBI AGCT 4 cut(s) 8, 125, 163, 487
AluI AGCT 4 cut(s) 8, 125, 163, 487
Alw21I GWGCWC 1 cut(s) 173
Alw26I GTCTC 2 cut(s) 28, 203
AlwI GGATC 1 cut(s) 406
AlwNI CAGNNNCTG 3 cut(s) 53, 307, 368
Ama87I CYCGRG 1 cut(s) 240
AoxI GGCC 1 cut(s) 474
ApaI GGGCCC 1 cut(s) 478
ApeKI GCWGC 5 cut(s) 122, 125, 371, 518, 566
ArsI GACNNNNNNTTYG 1 cut(s) 38
Asp700I GAANNNNTTC 1 cut(s) 224
AspA2I CCTAGG 1 cut(s) 442
AspS9I GGNCC 2 cut(s) 474, 475
AvaI CYCGRG 1 cut(s) 240
AvrII CCTAGG 1 cut(s) 442
BaeGI GKGCMC 2 cut(s) 68, 478
BanII GRGCYC 2 cut(s) 248, 478
BbsI GAAGAC 1 cut(s) 21
Bbv12I GWGCWC 1 cut(s) 173
BbvI GCAGC 5 cut(s) 112, 134, 358, 505, 553
BceAI ACGGC 1 cut(s) 84
BciT130I CCWGG 1 cut(s) 396
BcoDI GTCTC 2 cut(s) 28, 203
BfaI CTAG 2 cut(s) 44, 443
BglI GCCNNNNNGGC 1 cut(s) 446
BisI GCNGC 6 cut(s) 123, 126, 372, 450, 519, 567
BlnI CCTAGG 1 cut(s) 442
BlsI GCNGC 6 cut(s) 124, 127, 373, 451, 520, 568
Bme1390I CCNGG 1 cut(s) 396
BmeT110I CYCGRG 1 cut(s) 240
BmgT120I GGNCC 2 cut(s) 474, 475
BmiI GGNNCC 2 cut(s) 355, 476
BmrFI CCNGG 1 cut(s) 396
BmrI ACTGGG 1 cut(s) 70
BmsI GCATC 2 cut(s) 133, 199
BmuI ACTGGG 1 cut(s) 70
BpiI GAAGAC 1 cut(s) 21
BsaBI GATNNNNATC 1 cut(s) 534
BsaJI CCNNGG 1 cut(s) 442
BsaXI ACNNNNNCTCC 2 cut(s) 209, 239
Bsc4I CCNNNNNNNGG 2 cut(s) 321, 448
Bse118I RCCGGY 1 cut(s) 356
Bse1I ACTGG 1 cut(s) 65
Bse3DI GCAATG 1 cut(s) 219
Bse8I GATNNNNATC 1 cut(s) 534
BseBI CCWGG 1 cut(s) 396
BseDI CCNNGG 1 cut(s) 442
BseGI GGATG 1 cut(s) 523
BseJI GATNNNNATC 1 cut(s) 534
BseLI CCNNNNNNNGG 2 cut(s) 321, 448
BseMI GCAATG 1 cut(s) 219
BseNI ACTGG 1 cut(s) 65
BseSI GKGCMC 2 cut(s) 68, 478
BseXI GCAGC 5 cut(s) 112, 134, 358, 505, 553
BseYI CCCAGC 1 cut(s) 163
BsgI GTGCAG 1 cut(s) 504
Bsh1236I CGCG 1 cut(s) 21
BshFI GGCC 1 cut(s) 476
BsiHKAI GWGCWC 1 cut(s) 173
BsiHKCI CYCGRG 1 cut(s) 240
BsiSI CCGG 1 cut(s) 357
BslI CCNNNNNNNGG 2 cut(s) 321, 448
BsmAI GTCTC 2 cut(s) 28, 203
BsmBI CGTCTC 2 cut(s) 28, 203
BsnI GGCC 1 cut(s) 476
BsoBI CYCGRG 1 cut(s) 240
Bsp120I GGGCCC 1 cut(s) 474
Bsp1286I GDGCHC 4 cut(s) 68, 173, 248, 478
Bsp143I GATC 2 cut(s) 398, 535
BspACI CCGC 2 cut(s) 19, 449
BspANI GGCC 1 cut(s) 476
BspFNI CGCG 1 cut(s) 21
BspHI TCATGA 1 cut(s) 201
BspLI GGNNCC 2 cut(s) 355, 476
BspPI GGATC 1 cut(s) 406
BsrDI GCAATG 1 cut(s) 219
BsrFI RCCGGY 1 cut(s) 356
BsrI ACTGG 1 cut(s) 65
BssAI RCCGGY 1 cut(s) 356
BssECI CCNNGG 1 cut(s) 442
BssMI GATC 2 cut(s) 398, 535
BssT1I CCWWGG 1 cut(s) 442
Bst2UI CCWGG 1 cut(s) 396
Bst4CI ACNGT 3 cut(s) 55, 76, 107
Bst6I CTCTTC 1 cut(s) 215
BstAPI GCANNNNNTGC 1 cut(s) 307
BstC8I GCNNGC 5 cut(s) 94, 173, 312, 329, 414
BstF5I GGATG 1 cut(s) 523
BstFNI CGCG 1 cut(s) 21
BstKTI GATC 2 cut(s) 401, 538
BstMAI GTCTC 2 cut(s) 28, 203
BstMBI GATC 2 cut(s) 398, 535
BstMWI GCNNNNNNNGC 7 cut(s) 181, 307, 362, 371, 380, 413, 446
BstNI CCWGG 1 cut(s) 396
BstSCI CCNGG 1 cut(s) 394
BstSLI GKGCMC 2 cut(s) 68, 478
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 5 cut(s) 112, 134, 358, 505, 553
BstV2I GAAGAC 1 cut(s) 21
BstXI CCANNNNNNTGG 1 cut(s) 471
BsuRI GGCC 1 cut(s) 476
BtsCI GGATG 1 cut(s) 523
BtsI GCAGTG 1 cut(s) 366
BtsIMutI CAGTG 1 cut(s) 366
Cac8I GCNNGC 5 cut(s) 94, 173, 312, 329, 414
CaiI CAGNNNCTG 3 cut(s) 53, 307, 368
CciI TCATGA 1 cut(s) 201
Cfr10I RCCGGY 1 cut(s) 356
Cfr13I GGNCC 2 cut(s) 474, 475
CseI GACGC 2 cut(s) 10, 254
Csp6I GTAC 1 cut(s) 435
CviAII CATG 2 cut(s) 202, 332
CviQI GTAC 1 cut(s) 435
DpnI GATC 2 cut(s) 400, 537
DpnII GATC 2 cut(s) 398, 535
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
Eco130I CCWWGG 1 cut(s) 442
Eco24I GRGCYC 2 cut(s) 248, 478
Eco57I CTGAAG 1 cut(s) 114
Eco88I CYCGRG 1 cut(s) 240
EcoRII CCWGG 1 cut(s) 394
EcoT14I CCWWGG 1 cut(s) 442
EcoT38I GRGCYC 2 cut(s) 248, 478
ErhI CCWWGG 1 cut(s) 442
Esp3I CGTCTC 2 cut(s) 28, 203
FaeI CATG 2 cut(s) 205, 335
FaiI YATR 3 cut(s) 203, 299, 333
FatI CATG 2 cut(s) 201, 331
Fnu4HI GCNGC 6 cut(s) 123, 126, 372, 450, 519, 567
FokI GGATG 1 cut(s) 510
FriOI GRGCYC 2 cut(s) 248, 478
Fsp4HI GCNGC 6 cut(s) 123, 126, 372, 450, 519, 567
FspBI CTAG 2 cut(s) 44, 443
GluI GCNGC 6 cut(s) 123, 126, 372, 450, 519, 567
GsaI CCCAGC 1 cut(s) 167
HaeIII GGCC 1 cut(s) 476
HapII CCGG 1 cut(s) 357
HgaI GACGC 2 cut(s) 10, 254
Hin1II CATG 2 cut(s) 205, 335
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HinfI GANTC 3 cut(s) 304, 511, 530
HpaII CCGG 1 cut(s) 357
Hpy166II GTNNAC 3 cut(s) 58, 188, 279
Hpy188I TCNGA 2 cut(s) 39, 535
Hpy188III TCNNGA 3 cut(s) 11, 202, 527
Hpy8I GTNNAC 3 cut(s) 58, 188, 279
HpyAV CCTTC 2 cut(s) 490, 500
HpyCH4III ACNGT 3 cut(s) 55, 76, 107
HpyCH4IV ACGT 2 cut(s) 377, 423
HpyCH4V TGCA 8 cut(s) 92, 146, 212, 284, 301, 331, 521, 569
HpyF10VI GCNNNNNNNGC 7 cut(s) 181, 307, 362, 371, 380, 413, 446
HpySE526I ACGT 2 cut(s) 377, 423
Hsp92II CATG 2 cut(s) 205, 335
Kzo9I GATC 2 cut(s) 398, 535
LmnI GCTCC 3 cut(s) 13, 168, 353
Lsp1109I GCAGC 5 cut(s) 112, 134, 358, 505, 553
LweI GCATC 2 cut(s) 133, 199
MaeI CTAG 2 cut(s) 44, 443
MaeII ACGT 2 cut(s) 377, 423
MaeIII GTNAC 1 cut(s) 235
MalI GATC 2 cut(s) 400, 537
MboI GATC 2 cut(s) 398, 535
MboII GAAGA 3 cut(s) 26, 232, 596
MfeI CAATTG 1 cut(s) 570
MhlI GDGCHC 4 cut(s) 68, 173, 248, 478
MluCI AATT 1 cut(s) 570
MmeI TCCRAC 1 cut(s) 331
MnlI CCTC 1 cut(s) 216
MroXI GAANNNNTTC 1 cut(s) 224
MslI CAYNNNNRTG 2 cut(s) 83, 469
MspA1I CMGCKG 1 cut(s) 125
MspI CCGG 1 cut(s) 357
MspR9I CCNGG 1 cut(s) 396
MunI CAATTG 1 cut(s) 570
MvaI CCWGG 1 cut(s) 396
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 7 cut(s) 181, 307, 362, 371, 380, 413, 446
NdeII GATC 2 cut(s) 398, 535
NlaIII CATG 2 cut(s) 205, 335
NlaIV GGNNCC 2 cut(s) 355, 476
NmeAIII GCCGAG 1 cut(s) 340
NmuCI GTSAC 1 cut(s) 235
OliI CACNNNNGTG 1 cut(s) 469
PagI TCATGA 1 cut(s) 201
PdmI GAANNNNTTC 1 cut(s) 224
PfeI GAWTC 3 cut(s) 304, 511, 530
PflFI GACNNNGTC 1 cut(s) 20
PkrI GCNGC 6 cut(s) 124, 127, 373, 451, 520, 568
Psp6I CCWGG 1 cut(s) 394
PspFI CCCAGC 1 cut(s) 163
PspGI CCWGG 1 cut(s) 394
PspN4I GGNNCC 2 cut(s) 355, 476
PspOMI GGGCCC 1 cut(s) 474
PspPI GGNCC 2 cut(s) 474, 475
PstNI CAGNNNCTG 3 cut(s) 53, 307, 368
PsyI GACNNNGTC 1 cut(s) 20
PvuII CAGCTG 1 cut(s) 125
RsaI GTAC 1 cut(s) 436
RsaNI GTAC 1 cut(s) 435
RseI CAYNNNNRTG 2 cut(s) 83, 469
SatI GCNGC 6 cut(s) 123, 126, 372, 450, 519, 567
Sau3AI GATC 2 cut(s) 398, 535
Sau96I GGNCC 2 cut(s) 474, 475
ScrFI CCNGG 1 cut(s) 396
SduI GDGCHC 4 cut(s) 68, 173, 248, 478
SetI ASST 7 cut(s) 10, 127, 165, 227, 380, 426, 489
SfaNI GCATC 2 cut(s) 133, 199
SmiMI CAYNNNNRTG 2 cut(s) 83, 469
Sse9I AATT 1 cut(s) 570
SsiI CCGC 2 cut(s) 19, 449
SspMI CTAG 2 cut(s) 44, 443
StyD4I CCNGG 1 cut(s) 394
StyI CCWWGG 1 cut(s) 442
TaaI ACNGT 3 cut(s) 55, 76, 107
TaiI ACGT 2 cut(s) 380, 426
TaqI TCGA 1 cut(s) 248
TasI AATT 1 cut(s) 570
TauI GCSGC 1 cut(s) 452
TfiI GAWTC 3 cut(s) 304, 511, 530
TscAI CASTG 1 cut(s) 373
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 5 cut(s) 122, 125, 371, 518, 566
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 2 cut(s) 217, 519
TspRI CASTG 1 cut(s) 373
Tth111I GACNNNGTC 1 cut(s) 20
XmaJI CCTAGG 1 cut(s) 442
XmnI GAANNNNTTC 1 cut(s) 224
XspI CTAG 2 cut(s) 44, 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.