pycom06g15340

Tim17/Tim22/Tim23/Pmp24 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0006145
Physical Location & Seq
Forward (+)
19881538 .. 19882909
1372 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g15340.1

Sequence Viewer

Length: 612 bp
ATGGGAGCTCCGGAGGAATCCGAAGACACTGTCTCAAACCATTCTGCGAAACTAGCAGGAACTGTCAACTGGGGCACGGCAACTGTCATCGGAGTGTTTGCAGGCTTGTTTTATGGTGGTAGCAAGGAAGCAGCTGCATCAGTTAGCAAAGATGCAGAAGTAATGTTGAAGCTAGGGAGCACGCCAGACAAGCGTGAACAATATCGTCTCATGAGAGATGCAATGGAGAAAAGGTTCATTCGTGTCACTCGGGGCTCGATAGTTGGTGGAGTGCGCCTTGGATTGTTTACTGCTACTTTTTGTGGTATACAGAATCTGCTTGCTGAGAAAAGGGGCAAGCATGATGTTTTCAATGTTGTTGGAGCCGGTTCAGCCACTGCTGCTACGTTCGCTCTAATAATGCCTGGATCATTGGCTTGGCGAGCAAGAAACGTGATGCTAGGTGCTACCCTAGGAGCAGTGTTAGGTTTTCCACTAGGGTGGGCACATTTGAAGCTGGTAGGGAAGGCAAATGAAGGGAATCTGGCTGCACATCCTGATTCAGATCAAAGAGAAGCAAGGAGTGGTGTTGGTGCTGCAATTGAGAGGCTGGAAGATAACTTGAATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

204

Amino Acids

21.43

Weight (kDa)

9.33

Isoelectric Point (pI)

21.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 25 - 155 1.5e-10 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 307
AccIII TCCGGA 1 cut(s) 10
AclWI GGATC 1 cut(s) 415
AgsI TTSAA 4 cut(s) 169, 352, 493, 604
AjnI CCWGG 1 cut(s) 403
AleI CACNNNNGTG 1 cut(s) 478
AloI GAACNNNNNNTCC 2 cut(s) 218, 250
AluBI AGCT 4 cut(s) 8, 134, 172, 496
AluI AGCT 4 cut(s) 8, 134, 172, 496
Alw21I GWGCWC 2 cut(s) 10, 182
Alw26I GTCTC 2 cut(s) 37, 212
AlwI GGATC 1 cut(s) 415
AlwNI CAGNNNCTG 3 cut(s) 62, 316, 377
Ama87I CYCGRG 1 cut(s) 249
Aor13HI TCCGGA 1 cut(s) 10
ApeKI GCWGC 5 cut(s) 131, 134, 380, 527, 575
ArsI GACNNNNNNTTYG 2 cut(s) 15, 47
Asp700I GAANNNNTTC 1 cut(s) 233
AspA2I CCTAGG 1 cut(s) 451
AspLEI GCGC 1 cut(s) 276
AvaI CYCGRG 1 cut(s) 249
AvrII CCTAGG 1 cut(s) 451
BaeGI GKGCMC 2 cut(s) 77, 487
BanII GRGCYC 2 cut(s) 10, 257
BbsI GAAGAC 1 cut(s) 30
Bbv12I GWGCWC 2 cut(s) 10, 182
BbvI GCAGC 5 cut(s) 121, 143, 367, 514, 562
BceAI ACGGC 1 cut(s) 93
BciT130I CCWGG 1 cut(s) 405
BcoDI GTCTC 2 cut(s) 37, 212
BfaI CTAG 5 cut(s) 53, 173, 440, 452, 476
BisI GCNGC 5 cut(s) 132, 135, 381, 528, 576
BlnI CCTAGG 1 cut(s) 451
BlsI GCNGC 5 cut(s) 133, 136, 382, 529, 577
Bme1390I CCNGG 1 cut(s) 405
BmeT110I CYCGRG 1 cut(s) 249
BmiI GGNNCC 1 cut(s) 364
BmrFI CCNGG 1 cut(s) 405
BmrI ACTGGG 1 cut(s) 79
BmsI GCATC 4 cut(s) 142, 146, 208, 426
BmuI ACTGGG 1 cut(s) 79
BpiI GAAGAC 1 cut(s) 30
BsaBI GATNNNNATC 1 cut(s) 543
BsaJI CCNNGG 2 cut(s) 277, 451
BsaWI WCCGGW 1 cut(s) 10
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bse118I RCCGGY 1 cut(s) 365
Bse1I ACTGG 1 cut(s) 74
Bse3DI GCAATG 1 cut(s) 228
Bse8I GATNNNNATC 1 cut(s) 543
BseAI TCCGGA 1 cut(s) 10
BseBI CCWGG 1 cut(s) 405
BseDI CCNNGG 2 cut(s) 277, 451
BseGI GGATG 1 cut(s) 532
BseJI GATNNNNATC 1 cut(s) 543
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 1 cut(s) 315
BseNI ACTGG 1 cut(s) 74
BseSI GKGCMC 2 cut(s) 77, 487
BseXI GCAGC 5 cut(s) 121, 143, 367, 514, 562
BsgI GTGCAG 1 cut(s) 513
BsiHKAI GWGCWC 2 cut(s) 10, 182
BsiHKCI CYCGRG 1 cut(s) 249
BsiSI CCGG 2 cut(s) 11, 366
BsmAI GTCTC 2 cut(s) 37, 212
BsmBI CGTCTC 1 cut(s) 212
BsoBI CYCGRG 1 cut(s) 249
Bsp1286I GDGCHC 5 cut(s) 10, 77, 182, 257, 487
Bsp13I TCCGGA 1 cut(s) 10
Bsp143I GATC 2 cut(s) 407, 544
BspCNI CTCAG 1 cut(s) 316
BspEI TCCGGA 1 cut(s) 10
BspHI TCATGA 1 cut(s) 210
BspLI GGNNCC 1 cut(s) 364
BspPI GGATC 1 cut(s) 415
BsrDI GCAATG 1 cut(s) 228
BsrFI RCCGGY 1 cut(s) 365
BsrI ACTGG 1 cut(s) 74
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 2 cut(s) 277, 451
BssMI GATC 2 cut(s) 407, 544
BssNAI GTATAC 1 cut(s) 308
BssT1I CCWWGG 2 cut(s) 277, 451
Bst1107I GTATAC 1 cut(s) 308
Bst2UI CCWGG 1 cut(s) 405
Bst4CI ACNGT 3 cut(s) 31, 64, 85
BstC8I GCNNGC 5 cut(s) 103, 182, 321, 338, 423
BstDEI CTNAG 1 cut(s) 324
BstF5I GGATG 1 cut(s) 532
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 2 cut(s) 410, 547
BstMAI GTCTC 2 cut(s) 37, 212
BstMBI GATC 2 cut(s) 407, 544
BstMWI GCNNNNNNNGC 6 cut(s) 53, 190, 371, 380, 389, 422
BstNI CCWGG 1 cut(s) 405
BstSCI CCNGG 1 cut(s) 403
BstSLI GKGCMC 2 cut(s) 77, 487
BstV1I GCAGC 5 cut(s) 121, 143, 367, 514, 562
BstV2I GAAGAC 1 cut(s) 30
BstXI CCANNNNNNTGG 1 cut(s) 480
BstZ17I GTATAC 1 cut(s) 308
BtsCI GGATG 1 cut(s) 532
BtsI GCAGTG 2 cut(s) 375, 465
BtsIMutI CAGTG 3 cut(s) 27, 375, 465
Cac8I GCNNGC 5 cut(s) 103, 182, 321, 338, 423
CaiI CAGNNNCTG 3 cut(s) 62, 316, 377
CciI TCATGA 1 cut(s) 210
CfoI GCGC 1 cut(s) 276
Cfr10I RCCGGY 1 cut(s) 365
CviAII CATG 2 cut(s) 211, 341
DdeI CTNAG 1 cut(s) 324
DpnI GATC 2 cut(s) 409, 546
DpnII GATC 2 cut(s) 407, 544
Ecl136II GAGCTC 1 cut(s) 8
Eco130I CCWWGG 2 cut(s) 277, 451
Eco24I GRGCYC 2 cut(s) 10, 257
Eco53kI GAGCTC 1 cut(s) 8
Eco88I CYCGRG 1 cut(s) 249
EcoICRI GAGCTC 1 cut(s) 8
EcoRII CCWGG 1 cut(s) 403
EcoT14I CCWWGG 2 cut(s) 277, 451
EcoT38I GRGCYC 2 cut(s) 10, 257
ErhI CCWWGG 2 cut(s) 277, 451
Esp3I CGTCTC 1 cut(s) 212
FaeI CATG 2 cut(s) 214, 344
FaiI YATR 4 cut(s) 114, 212, 308, 342
FatI CATG 2 cut(s) 210, 340
FblI GTMKAC 1 cut(s) 307
Fnu4HI GCNGC 5 cut(s) 132, 135, 381, 528, 576
FokI GGATG 1 cut(s) 519
FriOI GRGCYC 2 cut(s) 10, 257
Fsp4HI GCNGC 5 cut(s) 132, 135, 381, 528, 576
FspBI CTAG 5 cut(s) 53, 173, 440, 452, 476
GlaI GCGC 1 cut(s) 275
GluI GCNGC 5 cut(s) 132, 135, 381, 528, 576
HapII CCGG 2 cut(s) 11, 366
HhaI GCGC 1 cut(s) 276
Hin1II CATG 2 cut(s) 214, 344
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HinfI GANTC 4 cut(s) 17, 313, 520, 539
HpaII CCGG 2 cut(s) 11, 366
Hpy166II GTNNAC 4 cut(s) 67, 197, 288, 308
Hpy188I TCNGA 3 cut(s) 22, 92, 544
Hpy188III TCNNGA 3 cut(s) 11, 211, 536
Hpy8I GTNNAC 4 cut(s) 67, 197, 288, 308
HpyAV CCTTC 2 cut(s) 499, 509
HpyCH4III ACNGT 3 cut(s) 31, 64, 85
HpyCH4IV ACGT 2 cut(s) 386, 432
HpyCH4V TGCA 6 cut(s) 101, 137, 155, 221, 530, 578
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 190, 371, 380, 389, 422
HpyF3I CTNAG 1 cut(s) 324
HpySE526I ACGT 2 cut(s) 386, 432
Hsp92II CATG 2 cut(s) 214, 344
HspAI GCGC 1 cut(s) 274
Kpn2I TCCGGA 1 cut(s) 10
Kzo9I GATC 2 cut(s) 407, 544
LmnI GCTCC 5 cut(s) 5, 13, 177, 362, 455
Lsp1109I GCAGC 5 cut(s) 121, 143, 367, 514, 562
LweI GCATC 4 cut(s) 142, 146, 208, 426
MaeI CTAG 5 cut(s) 53, 173, 440, 452, 476
MaeII ACGT 2 cut(s) 386, 432
MaeIII GTNAC 1 cut(s) 244
MalI GATC 2 cut(s) 409, 546
MboI GATC 2 cut(s) 407, 544
MboII GAAGA 2 cut(s) 35, 605
MfeI CAATTG 1 cut(s) 579
MhlI GDGCHC 5 cut(s) 10, 77, 182, 257, 487
MluCI AATT 1 cut(s) 579
MmeI TCCRAC 1 cut(s) 340
MnlI CCTC 2 cut(s) 7, 579
MroI TCCGGA 1 cut(s) 10
MroXI GAANNNNTTC 1 cut(s) 233
MslI CAYNNNNRTG 2 cut(s) 92, 478
MspA1I CMGCKG 1 cut(s) 134
MspI CCGG 2 cut(s) 11, 366
MspR9I CCNGG 1 cut(s) 405
MunI CAATTG 1 cut(s) 579
MvaI CCWGG 1 cut(s) 405
MwoI GCNNNNNNNGC 6 cut(s) 53, 190, 371, 380, 389, 422
NdeII GATC 2 cut(s) 407, 544
NlaIII CATG 2 cut(s) 214, 344
NlaIV GGNNCC 1 cut(s) 364
NmuCI GTSAC 1 cut(s) 244
OliI CACNNNNGTG 1 cut(s) 478
PagI TCATGA 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 233
PfeI GAWTC 4 cut(s) 17, 313, 520, 539
PflFI GACNNNGTC 1 cut(s) 29
PkrI GCNGC 5 cut(s) 133, 136, 382, 529, 577
Psp124BI GAGCTC 1 cut(s) 10
Psp6I CCWGG 1 cut(s) 403
PspGI CCWGG 1 cut(s) 403
PspN4I GGNNCC 1 cut(s) 364
PstNI CAGNNNCTG 3 cut(s) 62, 316, 377
PsyI GACNNNGTC 1 cut(s) 29
PvuII CAGCTG 1 cut(s) 134
RseI CAYNNNNRTG 2 cut(s) 92, 478
SacI GAGCTC 1 cut(s) 10
SatI GCNGC 5 cut(s) 132, 135, 381, 528, 576
Sau3AI GATC 2 cut(s) 407, 544
ScrFI CCNGG 1 cut(s) 405
SduI GDGCHC 5 cut(s) 10, 77, 182, 257, 487
SetI ASST 9 cut(s) 10, 136, 174, 236, 389, 435, 445, 469, 498
SfaNI GCATC 4 cut(s) 142, 146, 208, 426
SmiMI CAYNNNNRTG 2 cut(s) 92, 478
Sse9I AATT 1 cut(s) 579
SspMI CTAG 5 cut(s) 53, 173, 440, 452, 476
SstI GAGCTC 1 cut(s) 10
StyD4I CCNGG 1 cut(s) 403
StyI CCWWGG 2 cut(s) 277, 451
TaaI ACNGT 3 cut(s) 31, 64, 85
TaiI ACGT 2 cut(s) 389, 435
TaqI TCGA 1 cut(s) 257
TasI AATT 1 cut(s) 579
TfiI GAWTC 4 cut(s) 17, 313, 520, 539
TscAI CASTG 3 cut(s) 34, 382, 465
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 5 cut(s) 131, 134, 380, 527, 575
Tsp45I GTSAC 1 cut(s) 244
TspDTI ATGAA 2 cut(s) 226, 528
TspRI CASTG 3 cut(s) 34, 382, 465
Tth111I GACNNNGTC 1 cut(s) 29
XmaJI CCTAGG 1 cut(s) 451
XmiI GTMKAC 1 cut(s) 307
XmnI GAANNNNTTC 1 cut(s) 233
XspI CTAG 5 cut(s) 53, 173, 440, 452, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.