Rmu_ssc0000092.1_g000008

Tim17/Tim22/Tim23/Pmp24 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000092.1
OG0006145
Physical Location & Seq
Reverse (-)
35896 .. 37523
1628 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000092.1_g000008.1.cds

Sequence Viewer

Length: 612 bp
atggaagctggggagaaatcagaagaaactgtatcaaaacattctccgaaactagctggaactgtcaactggggaacagcaactgtcgccggagtatttgcaggcatgttgtacgggggtagcaaggaggcatctgcttcggttagcaaggatgctgaagtaatgttgaagcttgggagcacaccagacaagcgtgagcaatatcgtttgatgagagatgcaatggagaaacggttcattcgagtcacgcggggctcaatagttggtggagtgcgtcttggattgttcactgctaccttttatggtttacaaaatctgcttgctgagaaaaggggtgtgcaagatgttttcaatattgtgggagctggttcagccacggctgcgacattcgggctattattgccaggatcattacaatggcgtgcaagaaatatgttactgggttcgatccttggagcagtcattggtttcccaattgggtgggcacacttgaagctggtgcagatagcaaatgaagggaatcctgctgcgcatcctaattcagatcaaagagaagcaaagagtggtgttggtgctgccattgagaggcttgaagaaaatttgaataaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

203

Amino Acids

21.6

Weight (kDa)

9.55

Isoelectric Point (pI)

26.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 531
AccII CGCG 1 cut(s) 250
AciI CCGC 1 cut(s) 250
AclWI GGATC 2 cut(s) 415, 442
AcsI RAATTY 1 cut(s) 598
AcuI CTGAAG 1 cut(s) 177
AfaI GTAC 1 cut(s) 113
AfiI CCNNNNNNNGG 1 cut(s) 585
AgsI TTSAA 5 cut(s) 169, 352, 493, 593, 604
AjnI CCWGG 1 cut(s) 403
AloI GAACNNNNNNTCC 2 cut(s) 218, 250
AluBI AGCT 5 cut(s) 8, 56, 172, 365, 496
AluI AGCT 5 cut(s) 8, 56, 172, 365, 496
Alw21I GWGCWC 1 cut(s) 182
AlwI GGATC 2 cut(s) 415, 442
AlwNI CAGNNNCTG 1 cut(s) 83
ApeKI GCWGC 3 cut(s) 380, 527, 575
ApoI RAATTY 1 cut(s) 598
Asp700I GAANNNNTTC 1 cut(s) 233
AspLEI GCGC 1 cut(s) 532
BaeGI GKGCMC 1 cut(s) 487
BanII GRGCYC 1 cut(s) 257
Bbv12I GWGCWC 1 cut(s) 182
BbvI GCAGC 3 cut(s) 367, 514, 562
BceAI ACGGC 1 cut(s) 393
BcgI CGANNNNNNTGC 2 cut(s) 120, 154
BciT130I CCWGG 1 cut(s) 405
BfaI CTAG 1 cut(s) 53
BisI GCNGC 3 cut(s) 381, 528, 576
BlsI GCNGC 3 cut(s) 382, 529, 577
Bme1390I CCNGG 1 cut(s) 405
BmrFI CCNGG 1 cut(s) 405
BmrI ACTGGG 2 cut(s) 79, 449
BmsI GCATC 4 cut(s) 140, 142, 208, 541
BmuI ACTGGG 2 cut(s) 79, 449
BsaJI CCNNGG 2 cut(s) 375, 451
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 1 cut(s) 585
Bse1I ACTGG 2 cut(s) 74, 444
Bse3DI GCAATG 1 cut(s) 228
BseBI CCWGG 1 cut(s) 405
BseDI CCNNGG 2 cut(s) 375, 451
BseGI GGATG 2 cut(s) 157, 532
BseLI CCNNNNNNNGG 1 cut(s) 585
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 1 cut(s) 315
BseNI ACTGG 2 cut(s) 74, 444
BseSI GKGCMC 1 cut(s) 487
BseXI GCAGC 3 cut(s) 367, 514, 562
BseYI CCCAGC 1 cut(s) 8
BsgI GTGCAG 1 cut(s) 521
Bsh1236I CGCG 1 cut(s) 250
BsiHKAI GWGCWC 1 cut(s) 182
BsiSI CCGG 1 cut(s) 90
BslI CCNNNNNNNGG 1 cut(s) 585
Bsp1286I GDGCHC 3 cut(s) 182, 257, 487
Bsp143I GATC 3 cut(s) 407, 447, 544
BspACI CCGC 1 cut(s) 250
BspCNI CTCAG 1 cut(s) 316
BspFNI CGCG 1 cut(s) 250
BspPI GGATC 2 cut(s) 415, 442
BsrDI GCAATG 1 cut(s) 228
BsrI ACTGG 2 cut(s) 74, 444
BssECI CCNNGG 2 cut(s) 375, 451
BssMI GATC 3 cut(s) 407, 447, 544
BssT1I CCWWGG 1 cut(s) 451
Bst2UI CCWGG 1 cut(s) 405
Bst4CI ACNGT 4 cut(s) 31, 64, 85, 234
BstC8I GCNNGC 3 cut(s) 103, 321, 423
BstDEI CTNAG 1 cut(s) 324
BstDSI CCRYGG 1 cut(s) 375
BstF5I GGATG 2 cut(s) 157, 532
BstFNI CGCG 1 cut(s) 250
BstHHI GCGC 1 cut(s) 532
BstKTI GATC 3 cut(s) 410, 450, 547
BstMBI GATC 3 cut(s) 407, 447, 544
BstMWI GCNNNNNNNGC 4 cut(s) 86, 371, 380, 400
BstNI CCWGG 1 cut(s) 405
BstNSI RCATGY 1 cut(s) 109
BstSCI CCNGG 1 cut(s) 403
BstSLI GKGCMC 1 cut(s) 487
BstUI CGCG 1 cut(s) 250
BstV1I GCAGC 3 cut(s) 367, 514, 562
BstXI CCANNNNNNTGG 1 cut(s) 480
BtgI CCRYGG 1 cut(s) 375
BtsCI GGATG 2 cut(s) 157, 532
BtsI GCAGTG 1 cut(s) 288
BtsIMutI CAGTG 1 cut(s) 288
Cac8I GCNNGC 3 cut(s) 103, 321, 423
CaiI CAGNNNCTG 1 cut(s) 83
CfoI GCGC 1 cut(s) 532
CseI GACGC 1 cut(s) 263
Csp6I GTAC 1 cut(s) 112
CviAII CATG 1 cut(s) 106
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 1 cut(s) 324
DpnI GATC 3 cut(s) 409, 449, 546
DpnII GATC 3 cut(s) 407, 447, 544
Eco130I CCWWGG 1 cut(s) 451
Eco24I GRGCYC 1 cut(s) 257
Eco57I CTGAAG 1 cut(s) 177
EcoRII CCWGG 1 cut(s) 403
EcoT14I CCWWGG 1 cut(s) 451
EcoT38I GRGCYC 1 cut(s) 257
ErhI CCWWGG 1 cut(s) 451
FaeI CATG 1 cut(s) 109
FaiI YATR 3 cut(s) 107, 303, 434
FatI CATG 1 cut(s) 105
FauI CCCGC 1 cut(s) 243
Fnu4HI GCNGC 3 cut(s) 381, 528, 576
FokI GGATG 2 cut(s) 164, 519
FriOI GRGCYC 1 cut(s) 257
Fsp4HI GCNGC 3 cut(s) 381, 528, 576
FspBI CTAG 1 cut(s) 53
FspI TGCGCA 1 cut(s) 531
GlaI GCGC 1 cut(s) 531
GluI GCNGC 3 cut(s) 381, 528, 576
GsaI CCCAGC 1 cut(s) 12
HapII CCGG 1 cut(s) 90
HgaI GACGC 1 cut(s) 263
HhaI GCGC 1 cut(s) 532
Hin1II CATG 1 cut(s) 109
Hin6I GCGC 1 cut(s) 530
HinP1I GCGC 1 cut(s) 530
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 2 cut(s) 243, 520
HpaII CCGG 1 cut(s) 90
Hpy166II GTNNAC 3 cut(s) 67, 288, 308
Hpy188I TCNGA 3 cut(s) 22, 48, 544
Hpy8I GTNNAC 3 cut(s) 67, 288, 308
HpyAV CCTTC 1 cut(s) 509
HpyCH4III ACNGT 4 cut(s) 31, 64, 85, 234
HpyCH4V TGCA 5 cut(s) 101, 221, 340, 425, 502
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 371, 380, 400
HpyF3I CTNAG 1 cut(s) 324
Hsp92II CATG 1 cut(s) 109
HspAI GCGC 1 cut(s) 530
Kzo9I GATC 3 cut(s) 407, 447, 544
LmnI GCTCC 3 cut(s) 177, 362, 455
Lsp1109I GCAGC 3 cut(s) 367, 514, 562
LweI GCATC 4 cut(s) 140, 142, 208, 541
MaeI CTAG 1 cut(s) 53
MaeIII GTNAC 2 cut(s) 244, 435
MalI GATC 3 cut(s) 409, 449, 546
MboI GATC 3 cut(s) 407, 447, 544
MboII GAAGA 2 cut(s) 35, 605
MfeI CAATTG 1 cut(s) 474
MhlI GDGCHC 3 cut(s) 182, 257, 487
MluCI AATT 3 cut(s) 474, 538, 598
MlyI GAGTC 1 cut(s) 252
MnlI CCTC 2 cut(s) 121, 579
MroXI GAANNNNTTC 1 cut(s) 233
MslI CAYNNNNRTG 1 cut(s) 415
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 1 cut(s) 405
MunI CAATTG 1 cut(s) 474
MvaI CCWGG 1 cut(s) 405
MvnI CGCG 1 cut(s) 250
MwoI GCNNNNNNNGC 4 cut(s) 86, 371, 380, 400
NdeII GATC 3 cut(s) 407, 447, 544
NlaIII CATG 1 cut(s) 109
NmuCI GTSAC 1 cut(s) 244
NsbI TGCGCA 1 cut(s) 531
NspI RCATGY 1 cut(s) 109
PcsI WCGNNNNNNNCGW 1 cut(s) 238
PdmI GAANNNNTTC 1 cut(s) 233
PfeI GAWTC 1 cut(s) 520
PkrI GCNGC 3 cut(s) 382, 529, 577
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
Psp6I CCWGG 1 cut(s) 403
PspFI CCCAGC 1 cut(s) 8
PspGI CCWGG 1 cut(s) 403
PstNI CAGNNNCTG 1 cut(s) 83
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
RseI CAYNNNNRTG 1 cut(s) 415
SatI GCNGC 3 cut(s) 381, 528, 576
Sau3AI GATC 3 cut(s) 407, 447, 544
SchI GAGTC 1 cut(s) 252
ScrFI CCNGG 1 cut(s) 405
SduI GDGCHC 3 cut(s) 182, 257, 487
SetI ASST 6 cut(s) 10, 58, 174, 299, 367, 498
SfaNI GCATC 4 cut(s) 140, 142, 208, 541
SmiMI CAYNNNNRTG 1 cut(s) 415
Sse9I AATT 3 cut(s) 474, 538, 598
SsiI CCGC 1 cut(s) 250
SspI AATATT 1 cut(s) 355
SspMI CTAG 1 cut(s) 53
StyD4I CCNGG 1 cut(s) 403
StyI CCWWGG 1 cut(s) 451
TaaI ACNGT 4 cut(s) 31, 64, 85, 234
TaqI TCGA 2 cut(s) 241, 446
TasI AATT 3 cut(s) 474, 538, 598
TfiI GAWTC 1 cut(s) 520
TscAI CASTG 1 cut(s) 295
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 3 cut(s) 380, 527, 575
Tsp45I GTSAC 1 cut(s) 244
TspDTI ATGAA 2 cut(s) 226, 528
TspRI CASTG 1 cut(s) 295
XapI RAATTY 1 cut(s) 598
XceI RCATGY 1 cut(s) 109
XmnI GAANNNNTTC 1 cut(s) 233
XspI CTAG 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.