Rw7G011530

Tim17/Tim22/Tim23/Pmp24 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0006145
Physical Location & Seq
Forward (+)
10825918 .. 10828065
2148 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G011530.1

Sequence Viewer

Length: 471 bp
ATGTTCTACTTTTGTGGCTACGTTTCAACTTTATATACTCAAAGTTGTGAGGAAGTCTGCTTCTTTTGCCAGAGCAAGGATGCTGAAGTAATGTTGAAGCTTGGGAGCACACCAGACAAGCGTGAGCAATATCGTTTGATGAGAGATGCAATGGAGAAACGGTTCATTCGAGTCACGCGGGGCTCAATAGTTGGTGGAGTGCGTCTTGGATTGTTCACTGCTACCTTTTATGGTTTACAAAATCTGCTTGCTGAGAAAAGGGGTGTGCAAGATGTTTTCAATATTGTGGGAGCTGGTTCAGCCACGGCTGCGACATTCGGGCTATTATTGCCAGGATCATTACAATGGCGTGCAAGAAATATGTTACTGGGTTCGATCCTTGGAGCAGTCATTGGTTTCCCAATTGGTAATACATTCTACCTGTTGCTTCTCTTACACGTCTCAAACAATCCTATGCTGTTAACCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

156

Amino Acids

17.36

Weight (kDa)

9.21

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 46 - 131 2.4e-07 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 178
AciI CCGC 1 cut(s) 178
AclWI GGATC 2 cut(s) 343, 370
AcuI CTGAAG 1 cut(s) 105
AfiI CCNNNNNNNGG 1 cut(s) 76
AflIII ACRYGT 1 cut(s) 436
AgsI TTSAA 3 cut(s) 27, 97, 280
AjiI CACGTC 1 cut(s) 439
AjnI CCWGG 1 cut(s) 331
AloI GAACNNNNNNTCC 2 cut(s) 146, 178
AluBI AGCT 2 cut(s) 100, 293
AluI AGCT 2 cut(s) 100, 293
Alw21I GWGCWC 1 cut(s) 110
Alw26I GTCTC 1 cut(s) 445
AlwI GGATC 2 cut(s) 343, 370
ApeKI GCWGC 1 cut(s) 308
Asp700I GAANNNNTTC 1 cut(s) 161
BanII GRGCYC 1 cut(s) 185
Bbv12I GWGCWC 1 cut(s) 110
BbvI GCAGC 1 cut(s) 295
BceAI ACGGC 1 cut(s) 321
BciT130I CCWGG 1 cut(s) 333
BcoDI GTCTC 1 cut(s) 445
BisI GCNGC 1 cut(s) 309
BlsI GCNGC 1 cut(s) 310
Bme1390I CCNGG 1 cut(s) 333
BmgBI CACGTC 1 cut(s) 439
BmrFI CCNGG 1 cut(s) 333
BmrI ACTGGG 1 cut(s) 377
BmsI GCATC 2 cut(s) 70, 136
BmuI ACTGGG 1 cut(s) 377
BsaJI CCNNGG 2 cut(s) 303, 379
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse1I ACTGG 1 cut(s) 372
Bse3DI GCAATG 1 cut(s) 156
BseBI CCWGG 1 cut(s) 333
BseDI CCNNGG 2 cut(s) 303, 379
BseGI GGATG 1 cut(s) 85
BseLI CCNNNNNNNGG 1 cut(s) 76
BseMI GCAATG 1 cut(s) 156
BseMII CTCAG 1 cut(s) 243
BseNI ACTGG 1 cut(s) 372
BseXI GCAGC 1 cut(s) 295
Bsh1236I CGCG 1 cut(s) 178
BsiHKAI GWGCWC 1 cut(s) 110
BslI CCNNNNNNNGG 1 cut(s) 76
BsmAI GTCTC 1 cut(s) 445
BsmBI CGTCTC 1 cut(s) 445
Bsp1286I GDGCHC 2 cut(s) 110, 185
Bsp143I GATC 2 cut(s) 335, 375
BspACI CCGC 1 cut(s) 178
BspCNI CTCAG 1 cut(s) 244
BspFNI CGCG 1 cut(s) 178
BspPI GGATC 2 cut(s) 343, 370
BsrDI GCAATG 1 cut(s) 156
BsrI ACTGG 1 cut(s) 372
BssECI CCNNGG 2 cut(s) 303, 379
BssMI GATC 2 cut(s) 335, 375
BssT1I CCWWGG 1 cut(s) 379
Bst2UI CCWGG 1 cut(s) 333
Bst4CI ACNGT 1 cut(s) 162
BstC8I GCNNGC 2 cut(s) 249, 351
BstDEI CTNAG 1 cut(s) 252
BstDSI CCRYGG 1 cut(s) 303
BstF5I GGATG 1 cut(s) 85
BstFNI CGCG 1 cut(s) 178
BstKTI GATC 2 cut(s) 338, 378
BstMAI GTCTC 1 cut(s) 445
BstMBI GATC 2 cut(s) 335, 375
BstMWI GCNNNNNNNGC 4 cut(s) 66, 299, 308, 328
BstNI CCWGG 1 cut(s) 333
BstSCI CCNGG 1 cut(s) 331
BstUI CGCG 1 cut(s) 178
BstV1I GCAGC 1 cut(s) 295
BtgI CCRYGG 1 cut(s) 303
BtrI CACGTC 1 cut(s) 439
BtsCI GGATG 1 cut(s) 85
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 1 cut(s) 216
Cac8I GCNNGC 2 cut(s) 249, 351
CseI GACGC 1 cut(s) 191
CviJI RGCY 7 cut(s) 18, 100, 183, 293, 302, 308, 322
CviKI_1 RGCY 7 cut(s) 18, 100, 183, 293, 302, 308, 322
DdeI CTNAG 1 cut(s) 252
DpnI GATC 2 cut(s) 337, 377
DpnII GATC 2 cut(s) 335, 375
Eco130I CCWWGG 1 cut(s) 379
Eco24I GRGCYC 1 cut(s) 185
Eco57I CTGAAG 1 cut(s) 105
EcoRII CCWGG 1 cut(s) 331
EcoT14I CCWWGG 1 cut(s) 379
EcoT38I GRGCYC 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 379
Esp3I CGTCTC 1 cut(s) 445
FaiI YATR 5 cut(s) 34, 36, 231, 362, 455
FauI CCCGC 1 cut(s) 171
Fnu4HI GCNGC 1 cut(s) 309
FokI GGATG 1 cut(s) 92
FriOI GRGCYC 1 cut(s) 185
Fsp4HI GCNGC 1 cut(s) 309
GluI GCNGC 1 cut(s) 309
HgaI GACGC 1 cut(s) 191
HincII GTYRAC 1 cut(s) 462
HindII GTYRAC 1 cut(s) 462
HindIII AAGCTT 1 cut(s) 98
HinfI GANTC 1 cut(s) 171
HpaI GTTAAC 1 cut(s) 462
Hpy166II GTNNAC 3 cut(s) 216, 236, 462
Hpy8I GTNNAC 3 cut(s) 216, 236, 462
HpyCH4III ACNGT 1 cut(s) 162
HpyCH4IV ACGT 2 cut(s) 21, 438
HpyCH4V TGCA 3 cut(s) 149, 268, 353
HpyF10VI GCNNNNNNNGC 4 cut(s) 66, 299, 308, 328
HpyF3I CTNAG 1 cut(s) 252
HpySE526I ACGT 2 cut(s) 21, 438
KspAI GTTAAC 1 cut(s) 462
Kzo9I GATC 2 cut(s) 335, 375
LmnI GCTCC 3 cut(s) 105, 290, 383
LpnPI CCDG 7 cut(s) 83, 126, 279, 318, 345, 353, 434
Lsp1109I GCAGC 1 cut(s) 295
LweI GCATC 2 cut(s) 70, 136
MaeII ACGT 2 cut(s) 21, 438
MaeIII GTNAC 2 cut(s) 172, 363
MalI GATC 2 cut(s) 337, 377
MboI GATC 2 cut(s) 335, 375
MfeI CAATTG 1 cut(s) 402
MhlI GDGCHC 2 cut(s) 110, 185
MluCI AATT 1 cut(s) 402
MlyI GAGTC 1 cut(s) 180
MnlI CCTC 1 cut(s) 43
MroXI GAANNNNTTC 1 cut(s) 161
MseI TTAA 1 cut(s) 461
MslI CAYNNNNRTG 1 cut(s) 343
MspR9I CCNGG 1 cut(s) 333
MunI CAATTG 1 cut(s) 402
MvaI CCWGG 1 cut(s) 333
MvnI CGCG 1 cut(s) 178
MwoI GCNNNNNNNGC 4 cut(s) 66, 299, 308, 328
NdeII GATC 2 cut(s) 335, 375
NmuCI GTSAC 1 cut(s) 172
PcsI WCGNNNNNNNCGW 1 cut(s) 166
PdmI GAANNNNTTC 1 cut(s) 161
PkrI GCNGC 1 cut(s) 310
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
Psp6I CCWGG 1 cut(s) 331
PspGI CCWGG 1 cut(s) 331
RseI CAYNNNNRTG 1 cut(s) 343
SaqAI TTAA 1 cut(s) 461
SatI GCNGC 1 cut(s) 309
Sau3AI GATC 2 cut(s) 335, 375
SchI GAGTC 1 cut(s) 180
ScrFI CCNGG 1 cut(s) 333
SduI GDGCHC 2 cut(s) 110, 185
SetI ASST 7 cut(s) 24, 102, 227, 295, 423, 441, 467
SfaNI GCATC 2 cut(s) 70, 136
SmiMI CAYNNNNRTG 1 cut(s) 343
Sse9I AATT 1 cut(s) 402
SsiI CCGC 1 cut(s) 178
SspI AATATT 1 cut(s) 283
StyD4I CCNGG 1 cut(s) 331
StyI CCWWGG 1 cut(s) 379
TaaI ACNGT 1 cut(s) 162
TaiI ACGT 2 cut(s) 24, 441
TaqI TCGA 2 cut(s) 169, 374
TasI AATT 1 cut(s) 402
Tru1I TTAA 1 cut(s) 461
Tru9I TTAA 1 cut(s) 461
TscAI CASTG 1 cut(s) 223
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 1 cut(s) 308
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 1 cut(s) 154
TspRI CASTG 1 cut(s) 223
XmnI GAANNNNTTC 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.