AT5G66230

Belongs to the chalcone isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007083
Physical Location & Seq
Forward (+)
26461152 .. 26464171
3020 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66230.1

Sequence Viewer

Length: 990 bp
ATGGAGACACCATCATCGACAAGAAGAGTCACGAGATCTCAGACCCGCTGTGCTATCAATAATCTCTTATCCTCAAAGAAAGCGGATGATTGGTCAGATAAATGTCAATCGAAGTCGGTACAAAGAAACGGTGCAAAAGACAGGTCTGCGTTGTTTGACATAACCAACGACTCACCGATCGTAGGTCTGGCGATGCAGACACCGTCCTCAGGAGTTGTTGGGAAGAGGAACATGAGCAGAATCAATAACACTCCTGGATCTGGTGAGGCTCTCTTGAGAGGCCAAGTCAAGACTCTCTTGCAGAAGGTTGAGGAAGAAGCAGATCTCACCAAGATTTCGTTGGAATCTCGTCCTTTCATCCATCTCGTTACTTCTCCCATGGGTCTTCTCGCCCCAACTCCGGCCAACACCCCCCAAGTTCTTGGTTTCTCAGACGAAGTGCAGGTTGTGATCACTTCTCCGGTAGTTGCAGGACAATTCAGAGCACCGTCTCAGGTGGTGGAAAGCAACATGTTTGATGAGAAAGAAGAAAGCTTGGAGTTAGAGAAGAGCCCGAGCATTACTCGGTCATTGCTTCTGGACTTTAGTGATAAATCAGAATTATGGGAGAGCTCAGACTGCTCATCTGTGGTGACTCAAAACCCAGAGGATGATAACTCGTCGGTGTGGTCGATGCAGGCCAATGCAAGTGCCAAAGACGAAGAGTATGATGATGAAGAGGAAGAAGCCTATTCTTACGGTGAGGAATATGATGAGGAATACTACGACGAGGAAGAGGAGGAAGAAGAGGGAGGGATTGTGGATGGGTTGTGTGAGGGAATTAGGAAGATGAGCGTAGAGACTGACTTTGCAGGGAAACACACTAGATTTGTGTATGACAGTGAAGATGAAGAGATTGTGGAGGCCAAAGATCAATCTCCAGGAGTGTTGCGTTTGAAAGGCTTTCCTACACCGACAGGAAAACATGTCAGATTTGCCGGTGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00941 ko01100 ko01110 map00941 map01100 map01110
Pfam Domains
Protein Families
Chalcone

Protein Analysis

329

Amino Acids

36.43

Weight (kDa)

4.44

Isoelectric Point (pI)

61.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 433
AciI CCGC 2 cut(s) 46, 83
AclWI GGATC 1 cut(s) 265
AcoI YGGCCR 1 cut(s) 402
AfaI GTAC 1 cut(s) 120
AfiI CCNNNNNNNGG 4 cut(s) 182, 209, 260, 400
AflIII ACRYGT 2 cut(s) 510, 964
AgsI TTSAA 1 cut(s) 937
AjnI CCWGG 2 cut(s) 253, 919
AluBI AGCT 2 cut(s) 534, 612
AluI AGCT 2 cut(s) 534, 612
Alw21I GWGCWC 2 cut(s) 487, 614
Alw26I GTCTC 2 cut(s) 495, 833
AlwI GGATC 1 cut(s) 265
Ama87I CYCGRG 1 cut(s) 553
AoxI GGCC 4 cut(s) 280, 402, 678, 903
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
AsuHPI GGTGA 5 cut(s) 165, 275, 319, 643, 752
AvaI CYCGRG 1 cut(s) 553
AxyI CCTNAGG 1 cut(s) 208
BanII GRGCYC 2 cut(s) 554, 614
BauI CACGAG 1 cut(s) 31
BbsI GAAGAC 1 cut(s) 377
Bbv12I GWGCWC 2 cut(s) 487, 614
BccI CCATC 3 cut(s) 19, 369, 797
BciT130I CCWGG 2 cut(s) 255, 921
BclI TGATCA 1 cut(s) 450
BcoDI GTCTC 2 cut(s) 495, 833
BfaI CTAG 1 cut(s) 864
BfuAI ACCTGC 1 cut(s) 433
BglII AGATCT 2 cut(s) 35, 322
Bme1390I CCNGG 2 cut(s) 255, 921
BmeT110I CYCGRG 1 cut(s) 553
BmrFI CCNGG 2 cut(s) 255, 921
BmsI GCATC 2 cut(s) 183, 663
BpiI GAAGAC 1 cut(s) 377
BplI GAGNNNNNCTC 2 cut(s) 547, 579
BpmI CTGGAG 1 cut(s) 903
BpuEI CTTGAG 1 cut(s) 295
BsaJI CCNNGG 1 cut(s) 378
BsaWI WCCGGW 1 cut(s) 460
BsaXI ACNNNNNCTCC 2 cut(s) 783, 813
Bsc4I CCNNNNNNNGG 4 cut(s) 182, 209, 260, 400
Bse118I RCCGGY 1 cut(s) 977
Bse21I CCTNAGG 1 cut(s) 208
Bse3DI GCAATG 1 cut(s) 569
BseBI CCWGG 2 cut(s) 255, 921
BseDI CCNNGG 1 cut(s) 378
BseGI GGATG 4 cut(s) 91, 357, 655, 808
BseLI CCNNNNNNNGG 4 cut(s) 182, 209, 260, 400
BseMI GCAATG 1 cut(s) 569
BseMII CTCAG 5 cut(s) 53, 222, 444, 506, 627
BseRI GAGGAG 1 cut(s) 791
BsgI GTGCAG 1 cut(s) 461
Bsh1285I CGRYCG 1 cut(s) 180
BshFI GGCC 4 cut(s) 282, 404, 680, 905
BsiEI CGRYCG 1 cut(s) 180
BsiHKAI GWGCWC 2 cut(s) 487, 614
BsiHKCI CYCGRG 1 cut(s) 553
BsiSI CCGG 3 cut(s) 401, 461, 978
BslI CCNNNNNNNGG 4 cut(s) 182, 209, 260, 400
BsmAI GTCTC 2 cut(s) 495, 833
BsmBI CGTCTC 1 cut(s) 495
BsnI GGCC 4 cut(s) 282, 404, 680, 905
BsoBI CYCGRG 1 cut(s) 553
Bsp1286I GDGCHC 3 cut(s) 487, 554, 614
Bsp143I GATC 6 cut(s) 35, 177, 257, 322, 450, 910
Bsp19I CCATGG 1 cut(s) 378
BspACI CCGC 2 cut(s) 46, 83
BspANI GGCC 4 cut(s) 282, 404, 680, 905
BspCNI CTCAG 5 cut(s) 52, 221, 443, 505, 626
BspMI ACCTGC 1 cut(s) 433
BspPI GGATC 1 cut(s) 265
BspQI GCTCTTC 1 cut(s) 542
BsrDI GCAATG 1 cut(s) 569
BsrFI RCCGGY 1 cut(s) 977
BssAI RCCGGY 1 cut(s) 977
BssECI CCNNGG 1 cut(s) 378
BssMI GATC 6 cut(s) 35, 177, 257, 322, 450, 910
BssSI CACGAG 1 cut(s) 31
BssT1I CCWWGG 1 cut(s) 378
Bst2BI CACGAG 1 cut(s) 31
Bst2UI CCWGG 2 cut(s) 255, 921
Bst4CI ACNGT 5 cut(s) 131, 204, 489, 740, 881
Bst6I CTCTTC 8 cut(s) 19, 218, 542, 696, 711, 768, 780, 885
BstC8I GCNNGC 1 cut(s) 678
BstDEI CTNAG 5 cut(s) 39, 208, 430, 492, 613
BstDSI CCRYGG 1 cut(s) 378
BstF5I GGATG 4 cut(s) 91, 357, 655, 808
BstKTI GATC 6 cut(s) 38, 180, 260, 325, 453, 913
BstMAI GTCTC 2 cut(s) 495, 833
BstMBI GATC 6 cut(s) 35, 177, 257, 322, 450, 910
BstMCI CGRYCG 1 cut(s) 180
BstMWI GCNNNNNNNGC 1 cut(s) 618
BstNI CCWGG 2 cut(s) 255, 921
BstNSI RCATGY 2 cut(s) 514, 968
BstSCI CCNGG 2 cut(s) 253, 919
BstV2I GAAGAC 1 cut(s) 377
BstX2I RGATCY 3 cut(s) 35, 257, 322
BstXI CCANNNNNNTGG 1 cut(s) 422
BstYI RGATCY 3 cut(s) 35, 257, 322
Bsu36I CCTNAGG 1 cut(s) 208
BsuRI GGCC 4 cut(s) 282, 404, 680, 905
BtgI CCRYGG 1 cut(s) 378
BtgZI GCGATG 1 cut(s) 206
BtsCI GGATG 4 cut(s) 91, 357, 655, 808
BtsIMutI CAGTG 1 cut(s) 886
BveI ACCTGC 1 cut(s) 433
Cac8I GCNNGC 1 cut(s) 678
Cfr10I RCCGGY 1 cut(s) 977
Csp6I GTAC 1 cut(s) 119
CviAII CATG 4 cut(s) 232, 379, 511, 965
CviQI GTAC 1 cut(s) 119
DdeI CTNAG 5 cut(s) 39, 208, 430, 492, 613
DpnI GATC 6 cut(s) 37, 179, 259, 324, 452, 912
DpnII GATC 6 cut(s) 35, 177, 257, 322, 450, 910
EaeI YGGCCR 1 cut(s) 402
Eam1104I CTCTTC 8 cut(s) 19, 218, 542, 696, 711, 768, 780, 885
EarI CTCTTC 8 cut(s) 19, 218, 542, 696, 711, 768, 780, 885
Ecl136II GAGCTC 1 cut(s) 612
Eco130I CCWWGG 1 cut(s) 378
Eco24I GRGCYC 2 cut(s) 554, 614
Eco53kI GAGCTC 1 cut(s) 612
Eco81I CCTNAGG 1 cut(s) 208
Eco88I CYCGRG 1 cut(s) 553
EcoICRI GAGCTC 1 cut(s) 612
EcoRII CCWGG 2 cut(s) 253, 919
EcoT14I CCWWGG 1 cut(s) 378
EcoT38I GRGCYC 2 cut(s) 554, 614
ErhI CCWWGG 1 cut(s) 378
Esp3I CGTCTC 1 cut(s) 495
FaeI CATG 4 cut(s) 235, 382, 514, 968
FaiI YATR 9 cut(s) 161, 233, 380, 512, 604, 708, 750, 876, 966
FalI AAGNNNNNCTT 2 cut(s) 281, 313
FatI CATG 4 cut(s) 231, 378, 510, 964
FauI CCCGC 1 cut(s) 53
FbaI TGATCA 1 cut(s) 450
FokI GGATG 4 cut(s) 98, 344, 662, 815
FriOI GRGCYC 2 cut(s) 554, 614
FspBI CTAG 1 cut(s) 864
GsuI CTGGAG 1 cut(s) 903
HaeIII GGCC 4 cut(s) 282, 404, 680, 905
HapII CCGG 3 cut(s) 401, 461, 978
Hin1II CATG 4 cut(s) 235, 382, 514, 968
HindIII AAGCTT 1 cut(s) 532
HinfI GANTC 6 cut(s) 27, 170, 240, 292, 344, 634
HpaII CCGG 3 cut(s) 401, 461, 978
HphI GGTGA 5 cut(s) 165, 275, 319, 643, 752
Hpy188I TCNGA 7 cut(s) 42, 97, 433, 482, 598, 616, 971
Hpy188III TCNNGA 5 cut(s) 31, 210, 274, 289, 578
Hpy99I CGWCG 2 cut(s) 664, 770
HpyAV CCTTC 1 cut(s) 298
HpyCH4III ACNGT 5 cut(s) 131, 204, 489, 740, 881
HpyCH4V TGCA 8 cut(s) 134, 196, 301, 442, 470, 676, 686, 851
HpyF10VI GCNNNNNNNGC 1 cut(s) 618
HpyF3I CTNAG 5 cut(s) 39, 208, 430, 492, 613
Hsp92II CATG 4 cut(s) 235, 382, 514, 968
Ksp22I TGATCA 1 cut(s) 450
Kzo9I GATC 6 cut(s) 35, 177, 257, 322, 450, 910
LguI GCTCTTC 1 cut(s) 542
LweI GCATC 2 cut(s) 183, 663
MaeI CTAG 1 cut(s) 864
MaeIII GTNAC 3 cut(s) 28, 367, 631
MalI GATC 6 cut(s) 37, 179, 259, 324, 452, 912
MboI GATC 6 cut(s) 35, 177, 257, 322, 450, 910
MflI RGATCY 3 cut(s) 35, 257, 322
MhlI GDGCHC 3 cut(s) 487, 554, 614
MluCI AATT 3 cut(s) 476, 599, 819
MlyI GAGTC 4 cut(s) 36, 164, 286, 628
MmeI TCCRAC 1 cut(s) 321
MspA1I CMGCKG 1 cut(s) 48
MspI CCGG 3 cut(s) 401, 461, 978
MspR9I CCNGG 2 cut(s) 255, 921
MvaI CCWGG 2 cut(s) 255, 921
MwoI GCNNNNNNNGC 1 cut(s) 618
NcoI CCATGG 1 cut(s) 378
NdeII GATC 6 cut(s) 35, 177, 257, 322, 450, 910
NlaIII CATG 4 cut(s) 235, 382, 514, 968
NmuCI GTSAC 2 cut(s) 28, 631
NspI RCATGY 2 cut(s) 514, 968
PciI ACATGT 2 cut(s) 510, 964
PciSI GCTCTTC 1 cut(s) 542
PcsI WCGNNNNNNNCGW 1 cut(s) 668
PfeI GAWTC 2 cut(s) 240, 344
PflFI GACNNNGTC 1 cut(s) 202
PfoI TCCNGGA 2 cut(s) 253, 919
Ple19I CGATCG 1 cut(s) 180
PleI GAGTC 4 cut(s) 35, 164, 286, 628
PpsI GAGTC 4 cut(s) 35, 164, 286, 628
PscI ACATGT 2 cut(s) 510, 964
Psp124BI GAGCTC 1 cut(s) 614
Psp6I CCWGG 2 cut(s) 253, 919
PspGI CCWGG 2 cut(s) 253, 919
PsuI RGATCY 3 cut(s) 35, 257, 322
PsyI GACNNNGTC 1 cut(s) 202
PvuI CGATCG 1 cut(s) 180
RsaI GTAC 1 cut(s) 120
RsaNI GTAC 1 cut(s) 119
SacI GAGCTC 1 cut(s) 614
SapI GCTCTTC 1 cut(s) 542
Sau3AI GATC 6 cut(s) 35, 177, 257, 322, 450, 910
SchI GAGTC 4 cut(s) 36, 164, 286, 628
ScrFI CCNGG 2 cut(s) 255, 921
SduI GDGCHC 3 cut(s) 487, 554, 614
SetI ASST 7 cut(s) 146, 187, 309, 447, 498, 536, 614
SfaNI GCATC 2 cut(s) 183, 663
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
Sse9I AATT 3 cut(s) 476, 599, 819
SsiI CCGC 2 cut(s) 46, 83
SspMI CTAG 1 cut(s) 864
SstI GAGCTC 1 cut(s) 614
StyD4I CCNGG 2 cut(s) 253, 919
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 5 cut(s) 131, 204, 489, 740, 881
TaqI TCGA 3 cut(s) 17, 110, 671
TaqII GACCGA 1 cut(s) 555
TasI AATT 3 cut(s) 476, 599, 819
TfiI GAWTC 2 cut(s) 240, 344
TscAI CASTG 1 cut(s) 886
TseFI GTSAC 2 cut(s) 28, 631
Tsp45I GTSAC 2 cut(s) 28, 631
TspDTI ATGAA 3 cut(s) 346, 729, 903
TspRI CASTG 1 cut(s) 886
Tth111I GACNNNGTC 1 cut(s) 202
XceI RCATGY 2 cut(s) 514, 968
XcmI CCANNNNNNNNNTGG 1 cut(s) 337
XspI CTAG 1 cut(s) 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.