Rmu_sc0004888.1_g000020

chalcone-flavanone isomerase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004888.1
OG0007083
Physical Location & Seq
Reverse (-)
101859 .. 102362
504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004888.1_g000020.1.cds

Sequence Viewer

Length: 504 bp
atggaattgggcatgcgagctttgtgtttgtggtggtgggtgatgtgtggtcaacgacgattggggtccggtatcggtggtgacgtgacaaccctggtggtggctcttgtttggagctcaattcatggccttctcttctctgatcgacataaccaacgattctccgatagtgggggttgcgatgcagacgccatcatcggtcgtcctgtgaaactaaggagcatccgagtgaagaccgcactgggttccggcgaggctttgttgagaggtcaagtcaagaccctattgcaacaagtggaggaagaggcctctgtctctaagttctctttcgaaaacagaccttttcgacatcttcaaggccttattgggaaaacgctgacaggacttcttgccccaactccggcgaacacgccgcaggtgaatgatctcttggttgatagaagtgggagtaacattgagagaccctctcctctagtccaagaacaattgatctctcaggtttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

167

Amino Acids

18.27

Weight (kDa)

9.02

Isoelectric Point (pI)

52.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 406
Acc36I ACCTGC 1 cut(s) 406
AciI CCGC 2 cut(s) 237, 413
AcyI GRCGYC 1 cut(s) 189
AfiI CCNNNNNNNGG 3 cut(s) 100, 171, 400
AgsI TTSAA 1 cut(s) 356
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 1 cut(s) 93
AjuI GAANNNNNNNTTGG 2 cut(s) 413, 445
AluBI AGCT 2 cut(s) 20, 117
AluI AGCT 2 cut(s) 20, 117
Alw21I GWGCWC 1 cut(s) 119
Alw26I GTCTC 2 cut(s) 319, 454
AoxI GGCC 3 cut(s) 127, 306, 358
AspS9I GGNCC 1 cut(s) 66
AsuHPI GGTGA 3 cut(s) 52, 92, 430
AsuII TTCGAA 1 cut(s) 330
AvaII GGWCC 1 cut(s) 66
BanII GRGCYC 1 cut(s) 119
BbsI GAAGAC 1 cut(s) 239
Bbv12I GWGCWC 1 cut(s) 119
BccI CCATC 1 cut(s) 200
BciT130I CCWGG 1 cut(s) 95
BcoDI GTCTC 2 cut(s) 319, 454
BfaI CTAG 1 cut(s) 473
BfuAI ACCTGC 1 cut(s) 406
BisI GCNGC 1 cut(s) 413
BlsI GCNGC 1 cut(s) 414
Bme1390I CCNGG 1 cut(s) 95
Bme18I GGWCC 1 cut(s) 66
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 1 cut(s) 66
BmiI GGNNCC 2 cut(s) 67, 247
BmrFI CCNGG 1 cut(s) 95
BmrI ACTGGG 1 cut(s) 251
BmsI GCATC 2 cut(s) 172, 231
BmuI ACTGGG 1 cut(s) 251
BpiI GAAGAC 1 cut(s) 239
BplI GAGNNNNNCTC 4 cut(s) 449, 481, 451, 483
Bpu14I TTCGAA 1 cut(s) 330
BsaHI GRCGYC 1 cut(s) 189
BsaI GGTCTC 1 cut(s) 454
BsaJI CCNNGG 1 cut(s) 93
BsaWI WCCGGW 1 cut(s) 68
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 171, 400
Bse1I ACTGG 1 cut(s) 246
BseBI CCWGG 1 cut(s) 95
BseDI CCNNGG 1 cut(s) 93
BseGI GGATG 1 cut(s) 222
BseLI CCNNNNNNNGG 3 cut(s) 100, 171, 400
BseNI ACTGG 1 cut(s) 246
BseRI GAGGAG 1 cut(s) 459
Bsh1285I CGRYCG 1 cut(s) 202
BshFI GGCC 3 cut(s) 129, 308, 360
BsiEI CGRYCG 1 cut(s) 202
BsiHKAI GWGCWC 1 cut(s) 119
BsiSI CCGG 3 cut(s) 69, 249, 401
BslI CCNNNNNNNGG 3 cut(s) 100, 171, 400
BsmAI GTCTC 2 cut(s) 319, 454
BsnI GGCC 3 cut(s) 129, 308, 360
Bso31I GGTCTC 1 cut(s) 454
Bsp119I TTCGAA 1 cut(s) 330
Bsp1286I GDGCHC 1 cut(s) 119
Bsp143I GATC 3 cut(s) 142, 424, 489
BspACI CCGC 2 cut(s) 237, 413
BspANI GGCC 3 cut(s) 129, 308, 360
BspLI GGNNCC 2 cut(s) 67, 247
BspMI ACCTGC 1 cut(s) 406
BspT104I TTCGAA 1 cut(s) 330
BspTNI GGTCTC 1 cut(s) 454
BsrI ACTGG 1 cut(s) 246
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 3 cut(s) 142, 424, 489
BssNI GRCGYC 1 cut(s) 189
Bst2UI CCWGG 1 cut(s) 95
Bst6I CTCTTC 2 cut(s) 140, 297
BstACI GRCGYC 1 cut(s) 189
BstBI TTCGAA 1 cut(s) 330
BstC8I GCNNGC 2 cut(s) 14, 18
BstDEI CTNAG 3 cut(s) 215, 318, 495
BstF5I GGATG 1 cut(s) 222
BstKTI GATC 3 cut(s) 145, 427, 492
BstMAI GTCTC 2 cut(s) 319, 454
BstMBI GATC 3 cut(s) 142, 424, 489
BstMCI CGRYCG 1 cut(s) 202
BstNI CCWGG 1 cut(s) 95
BstNSI RCATGY 1 cut(s) 16
BstSCI CCNGG 1 cut(s) 93
BstV2I GAAGAC 1 cut(s) 239
BsuRI GGCC 3 cut(s) 129, 308, 360
BtgZI GCGATG 1 cut(s) 195
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 1 cut(s) 222
BtsIMutI CAGTG 1 cut(s) 239
BveI ACCTGC 1 cut(s) 406
Cac8I GCNNGC 2 cut(s) 14, 18
Cfr13I GGNCC 1 cut(s) 66
CseI GACGC 1 cut(s) 197
CspCI CAANNNNNGTGG 2 cut(s) 78, 113
CviAII CATG 2 cut(s) 13, 125
CviJI RGCY 7 cut(s) 20, 104, 117, 129, 257, 308, 360
CviKI_1 RGCY 7 cut(s) 20, 104, 117, 129, 257, 308, 360
DdeI CTNAG 3 cut(s) 215, 318, 495
DpnI GATC 3 cut(s) 144, 426, 491
DpnII GATC 3 cut(s) 142, 424, 489
Eam1104I CTCTTC 2 cut(s) 140, 297
EarI CTCTTC 2 cut(s) 140, 297
Ecl136II GAGCTC 1 cut(s) 117
Eco147I AGGCCT 2 cut(s) 308, 360
Eco24I GRGCYC 1 cut(s) 119
Eco31I GGTCTC 1 cut(s) 454
Eco47I GGWCC 1 cut(s) 66
Eco53kI GAGCTC 1 cut(s) 117
EcoICRI GAGCTC 1 cut(s) 117
EcoRII CCWGG 1 cut(s) 93
EcoT38I GRGCYC 1 cut(s) 119
FaeI CATG 2 cut(s) 16, 128
FaiI YATR 3 cut(s) 14, 126, 150
FatI CATG 2 cut(s) 12, 124
Fnu4HI GCNGC 1 cut(s) 413
FokI GGATG 1 cut(s) 209
FriOI GRGCYC 1 cut(s) 119
Fsp4HI GCNGC 1 cut(s) 413
FspBI CTAG 1 cut(s) 473
GluI GCNGC 1 cut(s) 413
HaeIII GGCC 3 cut(s) 129, 308, 360
HapII CCGG 3 cut(s) 69, 249, 401
HgaI GACGC 1 cut(s) 197
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 2 cut(s) 16, 128
HincII GTYRAC 1 cut(s) 53
HindII GTYRAC 1 cut(s) 53
HinfI GANTC 1 cut(s) 159
HpaII CCGG 3 cut(s) 69, 249, 401
HphI GGTGA 3 cut(s) 52, 92, 430
Hpy166II GTNNAC 1 cut(s) 53
Hpy188I TCNGA 3 cut(s) 142, 166, 227
Hpy188III TCNNGA 1 cut(s) 277
Hpy8I GTNNAC 1 cut(s) 53
Hpy99I CGWCG 1 cut(s) 60
HpyAV CCTTC 1 cut(s) 140
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 2 cut(s) 185, 289
HpyF3I CTNAG 3 cut(s) 215, 318, 495
HpySE526I ACGT 1 cut(s) 84
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 2 cut(s) 16, 128
Kzo9I GATC 3 cut(s) 142, 424, 489
LmnI GCTCC 2 cut(s) 114, 219
LweI GCATC 2 cut(s) 172, 231
MaeI CTAG 1 cut(s) 473
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 3 cut(s) 80, 85, 449
MalI GATC 3 cut(s) 144, 426, 491
MboI GATC 3 cut(s) 142, 424, 489
MboII GAAGA 4 cut(s) 127, 244, 314, 344
MfeI CAATTG 1 cut(s) 485
MhlI GDGCHC 1 cut(s) 119
MluCI AATT 3 cut(s) 5, 120, 485
MnlI CCTC 7 cut(s) 247, 260, 292, 298, 319, 475, 480
MslI CAYNNNNRTG 1 cut(s) 227
MspI CCGG 3 cut(s) 69, 249, 401
MspR9I CCNGG 1 cut(s) 95
MunI CAATTG 1 cut(s) 485
MvaI CCWGG 1 cut(s) 95
NdeII GATC 3 cut(s) 142, 424, 489
NlaIII CATG 2 cut(s) 16, 128
NlaIV GGNNCC 2 cut(s) 67, 247
NmuCI GTSAC 2 cut(s) 80, 85
NspI RCATGY 1 cut(s) 16
NspV TTCGAA 1 cut(s) 330
PaeI GCATGC 1 cut(s) 16
PaqCI CACCTGC 1 cut(s) 406
PceI AGGCCT 2 cut(s) 308, 360
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PfeI GAWTC 1 cut(s) 159
PkrI GCNGC 1 cut(s) 414
Psp124BI GAGCTC 1 cut(s) 119
Psp6I CCWGG 1 cut(s) 93
PspGI CCWGG 1 cut(s) 93
PspN4I GGNNCC 2 cut(s) 67, 247
PspPI GGNCC 1 cut(s) 66
RseI CAYNNNNRTG 1 cut(s) 227
SacI GAGCTC 1 cut(s) 119
SatI GCNGC 1 cut(s) 413
Sau3AI GATC 3 cut(s) 142, 424, 489
Sau96I GGNCC 1 cut(s) 66
ScrFI CCNGG 1 cut(s) 95
SduI GDGCHC 1 cut(s) 119
SetI ASST 7 cut(s) 22, 87, 119, 271, 343, 420, 501
SfaNI GCATC 2 cut(s) 172, 231
SfuI TTCGAA 1 cut(s) 330
SinI GGWCC 1 cut(s) 66
SmiMI CAYNNNNRTG 1 cut(s) 227
SphI GCATGC 1 cut(s) 16
Sse9I AATT 3 cut(s) 5, 120, 485
SseBI AGGCCT 2 cut(s) 308, 360
SsiI CCGC 2 cut(s) 237, 413
SspMI CTAG 1 cut(s) 473
SstI GAGCTC 1 cut(s) 119
StuI AGGCCT 2 cut(s) 308, 360
StyD4I CCNGG 1 cut(s) 93
TaiI ACGT 1 cut(s) 87
TaqI TCGA 3 cut(s) 145, 330, 346
TaqII GACCGA 1 cut(s) 188
TasI AATT 3 cut(s) 5, 120, 485
TauI GCSGC 1 cut(s) 415
TfiI GAWTC 1 cut(s) 159
TscAI CASTG 1 cut(s) 246
TseFI GTSAC 2 cut(s) 80, 85
Tsp45I GTSAC 2 cut(s) 80, 85
TspDTI ATGAA 1 cut(s) 113
TspRI CASTG 1 cut(s) 246
VpaK11BI GGWCC 1 cut(s) 66
XceI RCATGY 1 cut(s) 16
XspI CTAG 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.