ATMG00510

Respiratory-chain NADH dehydrogenase, 49 Kd subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
Mt
OG0006041
Physical Location & Seq
Forward (+)
132071 .. 138153
6083 bp
Loading structure...
UTR
Exon/CDS
Intron
ATMG00510.1

Sequence Viewer

Length: 1185 bp
ATGACGACTAGGAAAAGGCAAATCAAAAATTTTACTTCGAATTTTGGACCTCAACATCCTGCTGCTCATGGTGTTTCACGATTAGTATTGGAAATGAACGGAGAAGTGGTGGAACGTGCGGAACCACATATTGGATCACTCCATAGAGGGACTGAGAAATTAATAGAGTACAAAACTTATCTTCAAGCTTTACCTTATTCTGATCGTTCAGACTATGTTTCTATGATGGCCCAAGAACACGCTCATTCTTCAGCTGTAGAGAAACTTTTGAATTGCGAGGTACCATTACGAGCTCAATATATACGAGTGTTATTCCGTGAAATAACTCGAATTTCAAATCATTCACTTGCTTTAACTACTCATGCTATGGATGTGGGAGCATTAACTCCGTTCCTGTGGGCTTTTGAGGAGCGGGAGAAATTGTTGGAATTCTATGAAAGAGTCTCGGGAGCCAGGATGCATGCCAGTTTCATACGACCAGGTGGAGTGGCACAAGATCTGCCTCTTGGCTTATGTCGAGATATTGATTCCTTCACACAACAATTTGCTTCTCGTATCGATGAATTAGAAGAGATGTCAACCGGCAACCGTATCTGGAAACAACGATTAGTGGATATTGGTACTGTCACTGCACAGCAAGCAAAGGATTGGGGATTCAGTGGTGTAATGTTAAGAGGTCCAGGGGTATGCTGGGATTCGCGAAGAGCAGCACCTTACGATGTTCATGACCAATCGGATCTTGACGTACCAGTAGGTACCAGAGGAGATCGCTATGATCGTTACTGTATCCGTATCGAAGAGATGCGACAAAGTCTTCGGATCATTGTGCAATGTCTTAATCAAATGCCTAGCGGCATGATCAAAGCCGATGATCGTAAGCTATGTCCTCCATCACGATGTCGAATGAAACTATCCATGGAATCCTCAATTCACCATTTCGAACTTTATACAGAAGGTTTTTCCGTACCAGCTTCTTCTACCTATACCGCAGTTGAAGCACCTAAAGGAGAATTTGGTGTCTTTCTGGTCAGTAATGGAAGCAATCGTCCCTACCGTCGTAAAATAAGAGCACCCGGCTCTGCCCATTCACAAGGACTCGATTCTATGTCCAAACATCACATGCCAGCAGATGTGGTCACCATCATAGGTACTCAAGATATTGTGTTTGGAGAGGTGGATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
Complex1_49kDa

Protein Analysis

394

Amino Acids

44.58

Weight (kDa)

7.7

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Complex1_49kDa PF00346 124 - 394 7.4e-110 Respiratory-chain NADH dehydrogenase, 49 Kd subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 280, 755
AccB1I GGYRCC 2 cut(s) 280, 755
AccB7I CCANNNNNTGG 1 cut(s) 131
AccBSI CCGCTC 1 cut(s) 412
AccII CGCG 1 cut(s) 700
AciI CCGC 4 cut(s) 119, 412, 852, 987
AclWI GGATC 3 cut(s) 142, 744, 827
AcsI RAATTY 5 cut(s) 28, 40, 330, 428, 1010
AcuI CTGAAG 1 cut(s) 234
AfaI GTAC 7 cut(s) 170, 282, 622, 747, 757, 966, 1150
AfiI CCNNNNNNNGG 1 cut(s) 131
AgsI TTSAA 4 cut(s) 185, 271, 336, 995
AjnI CCWGG 3 cut(s) 452, 478, 679
AjuI GAANNNNNNNTTGG 2 cut(s) 114, 146
AluBI AGCT 5 cut(s) 188, 254, 293, 880, 971
AluI AGCT 5 cut(s) 188, 254, 293, 880, 971
Alw21I GWGCWC 2 cut(s) 295, 1072
Alw26I GTCTC 1 cut(s) 448
AlwI GGATC 3 cut(s) 142, 744, 827
Ama87I CYCGRG 1 cut(s) 445
AoxI GGCC 1 cut(s) 228
ApeKI GCWGC 2 cut(s) 62, 707
ApoI RAATTY 5 cut(s) 28, 40, 330, 428, 1010
ArsI GACNNNNNNTTYG 2 cut(s) 798, 830
AseI ATTAAT 1 cut(s) 161
Asp718I GGTACC 2 cut(s) 280, 755
AspS9I GGNCC 3 cut(s) 47, 229, 677
AsuC2I CCSGG 1 cut(s) 1074
AsuHPI GGTGA 2 cut(s) 923, 1129
AsuII TTCGAA 2 cut(s) 38, 939
AvaI CYCGRG 1 cut(s) 445
AvaII GGWCC 2 cut(s) 47, 677
BanI GGYRCC 2 cut(s) 280, 755
BanII GRGCYC 1 cut(s) 295
BbsI GAAGAC 1 cut(s) 806
Bbv12I GWGCWC 2 cut(s) 295, 1072
BbvI GCAGC 2 cut(s) 49, 719
BccI CCATC 3 cut(s) 220, 898, 1148
BciT130I CCWGG 3 cut(s) 454, 480, 681
BciVI GTATCC 1 cut(s) 797
BclI TGATCA 1 cut(s) 858
BcnI CCSGG 1 cut(s) 1074
BcoDI GTCTC 1 cut(s) 448
BfaI CTAG 2 cut(s) 9, 849
BfmI CTRYAG 1 cut(s) 255
BfuI GTATCC 1 cut(s) 797
BglII AGATCT 1 cut(s) 496
BisI GCNGC 3 cut(s) 63, 708, 853
BlsI GCNGC 3 cut(s) 64, 709, 854
Bme1390I CCNGG 4 cut(s) 454, 480, 681, 1074
Bme18I GGWCC 2 cut(s) 47, 677
BmeT110I CYCGRG 1 cut(s) 445
BmgT120I GGNCC 3 cut(s) 47, 229, 677
BmiI GGNNCC 4 cut(s) 123, 282, 451, 757
BmrFI CCNGG 4 cut(s) 454, 480, 681, 1074
BmsI GCATC 2 cut(s) 447, 792
BpiI GAAGAC 1 cut(s) 806
Bpu14I TTCGAA 2 cut(s) 38, 939
BpuEI CTTGAG 1 cut(s) 1137
BpuMI CCSGG 1 cut(s) 1074
Bsa29I ATCGAT 1 cut(s) 558
BsaJI CCNNGG 2 cut(s) 680, 915
BsaXI ACNNNNNCTCC 4 cut(s) 93, 123, 407, 437
Bsc4I CCNNNNNNNGG 1 cut(s) 131
Bse118I RCCGGY 1 cut(s) 581
Bse1I ACTGG 2 cut(s) 465, 749
Bse3DI GCAATG 1 cut(s) 836
BseBI CCWGG 3 cut(s) 454, 480, 681
BseCI ATCGAT 1 cut(s) 558
BseDI CCNNGG 2 cut(s) 680, 915
BseGI GGATG 3 cut(s) 55, 376, 462
BseLI CCNNNNNNNGG 1 cut(s) 131
BseMI GCAATG 1 cut(s) 836
BseMII CTCAG 1 cut(s) 144
BseNI ACTGG 2 cut(s) 465, 749
BseRI GAGGAG 2 cut(s) 422, 777
BseXI GCAGC 2 cut(s) 49, 719
BseYI CCCAGC 1 cut(s) 690
BsgI GTGCAG 1 cut(s) 615
Bsh1236I CGCG 1 cut(s) 700
BshFI GGCC 1 cut(s) 230
BshNI GGYRCC 2 cut(s) 280, 755
BshVI ATCGAT 1 cut(s) 558
BsiHKAI GWGCWC 2 cut(s) 295, 1072
BsiHKCI CYCGRG 1 cut(s) 445
BsiSI CCGG 2 cut(s) 582, 1074
BslFI GGGAC 2 cut(s) 163, 1032
BslI CCNNNNNNNGG 1 cut(s) 131
BsmAI GTCTC 1 cut(s) 448
BsmFI GGGAC 2 cut(s) 163, 1032
BsnI GGCC 1 cut(s) 230
BsoBI CYCGRG 1 cut(s) 445
Bsp119I TTCGAA 2 cut(s) 38, 939
Bsp1286I GDGCHC 2 cut(s) 295, 1072
Bsp143I GATC 9 cut(s) 134, 202, 496, 736, 766, 775, 819, 858, 871
Bsp19I CCATGG 1 cut(s) 915
Bsp68I TCGCGA 1 cut(s) 700
BspACI CCGC 4 cut(s) 119, 412, 852, 987
BspANI GGCC 1 cut(s) 230
BspCNI CTCAG 1 cut(s) 145
BspDI ATCGAT 1 cut(s) 558
BspFNI CGCG 1 cut(s) 700
BspHI TCATGA 1 cut(s) 724
BspLI GGNNCC 4 cut(s) 123, 282, 451, 757
BspPI GGATC 3 cut(s) 142, 744, 827
BspQI GCTCTTC 1 cut(s) 697
BspT104I TTCGAA 2 cut(s) 38, 939
BspT107I GGYRCC 2 cut(s) 280, 755
BsrBI CCGCTC 1 cut(s) 412
BsrDI GCAATG 1 cut(s) 836
BsrFI RCCGGY 1 cut(s) 581
BsrI ACTGG 2 cut(s) 465, 749
BssAI RCCGGY 1 cut(s) 581
BssECI CCNNGG 2 cut(s) 680, 915
BssMI GATC 9 cut(s) 134, 202, 496, 736, 766, 775, 819, 858, 871
BssT1I CCWWGG 1 cut(s) 915
Bst2UI CCWGG 3 cut(s) 454, 480, 681
Bst4CI ACNGT 4 cut(s) 590, 625, 785, 1055
Bst6I CTCTTC 3 cut(s) 564, 697, 792
BstBI TTCGAA 2 cut(s) 38, 939
BstC8I GCNNGC 3 cut(s) 462, 639, 1125
BstDEI CTNAG 1 cut(s) 153
BstDSI CCRYGG 1 cut(s) 915
BstEII GGTNACC 1 cut(s) 1135
BstF5I GGATG 3 cut(s) 55, 376, 462
BstFNI CGCG 1 cut(s) 700
BstKTI GATC 9 cut(s) 137, 205, 499, 739, 769, 778, 822, 861, 874
BstMAI GTCTC 1 cut(s) 448
BstMBI GATC 9 cut(s) 134, 202, 496, 736, 766, 775, 819, 858, 871
BstMWI GCNNNNNNNGC 2 cut(s) 638, 995
BstNI CCWGG 3 cut(s) 454, 480, 681
BstNSI RCATGY 2 cut(s) 464, 1123
BstPI GGTNACC 1 cut(s) 1135
BstSCI CCNGG 4 cut(s) 452, 478, 679, 1072
BstSFI CTRYAG 1 cut(s) 255
BstUI CGCG 1 cut(s) 700
BstV1I GCAGC 2 cut(s) 49, 719
BstV2I GAAGAC 1 cut(s) 806
BstX2I RGATCY 2 cut(s) 496, 736
BstYI RGATCY 2 cut(s) 496, 736
Bsu15I ATCGAT 1 cut(s) 558
BsuI GTATCC 1 cut(s) 797
BsuRI GGCC 1 cut(s) 230
BsuTUI ATCGAT 1 cut(s) 558
BtgI CCRYGG 1 cut(s) 915
BtsCI GGATG 3 cut(s) 55, 376, 462
BtsI GCAGTG 1 cut(s) 627
BtsIMutI CAGTG 2 cut(s) 627, 664
BtuMI TCGCGA 1 cut(s) 700
Cac8I GCNNGC 3 cut(s) 462, 639, 1125
CciI TCATGA 1 cut(s) 724
Cfr10I RCCGGY 1 cut(s) 581
Cfr13I GGNCC 3 cut(s) 47, 229, 677
ClaI ATCGAT 1 cut(s) 558
CsiI ACCWGGT 1 cut(s) 478
Csp6I GTAC 7 cut(s) 169, 281, 621, 746, 756, 965, 1149
CviAII CATG 7 cut(s) 68, 362, 461, 725, 856, 916, 1120
CviQI GTAC 7 cut(s) 169, 281, 621, 746, 756, 965, 1149
DdeI CTNAG 1 cut(s) 153
DpnI GATC 9 cut(s) 136, 204, 498, 738, 768, 777, 821, 860, 873
DpnII GATC 9 cut(s) 134, 202, 496, 736, 766, 775, 819, 858, 871
Eam1104I CTCTTC 3 cut(s) 564, 697, 792
EarI CTCTTC 3 cut(s) 564, 697, 792
Ecl136II GAGCTC 1 cut(s) 293
Eco130I CCWWGG 1 cut(s) 915
Eco24I GRGCYC 1 cut(s) 295
Eco47I GGWCC 2 cut(s) 47, 677
Eco53kI GAGCTC 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 234
Eco88I CYCGRG 1 cut(s) 445
Eco91I GGTNACC 1 cut(s) 1135
EcoICRI GAGCTC 1 cut(s) 293
EcoO65I GGTNACC 1 cut(s) 1135
EcoRI GAATTC 1 cut(s) 428
EcoRII CCWGG 3 cut(s) 452, 478, 679
EcoT14I CCWWGG 1 cut(s) 915
EcoT22I ATGCAT 1 cut(s) 462
EcoT38I GRGCYC 1 cut(s) 295
ErhI CCWWGG 1 cut(s) 915
FaeI CATG 7 cut(s) 71, 365, 464, 728, 859, 919, 1123
FaqI GGGAC 2 cut(s) 163, 1032
FatI CATG 7 cut(s) 67, 361, 460, 724, 855, 915, 1119
FauI CCCGC 1 cut(s) 405
FbaI TGATCA 1 cut(s) 858
Fnu4HI GCNGC 3 cut(s) 63, 708, 853
FokI GGATG 3 cut(s) 42, 383, 469
FriOI GRGCYC 1 cut(s) 295
Fsp4HI GCNGC 3 cut(s) 63, 708, 853
FspBI CTAG 2 cut(s) 9, 849
GluI GCNGC 3 cut(s) 63, 708, 853
GsaI CCCAGC 1 cut(s) 694
HaeIII GGCC 1 cut(s) 230
HapII CCGG 2 cut(s) 582, 1074
Hin1II CATG 7 cut(s) 71, 365, 464, 728, 859, 919, 1123
HincII GTYRAC 1 cut(s) 579
HindII GTYRAC 1 cut(s) 579
HindIII AAGCTT 1 cut(s) 186
HinfI GANTC 7 cut(s) 441, 527, 654, 695, 920, 1095, 1100
HpaII CCGG 2 cut(s) 582, 1074
HphI GGTGA 2 cut(s) 923, 1129
Hpy166II GTNNAC 1 cut(s) 579
Hpy188I TCNGA 4 cut(s) 202, 211, 736, 819
Hpy188III TCNNGA 9 cut(s) 78, 447, 518, 595, 699, 725, 740, 894, 1154
Hpy8I GTNNAC 1 cut(s) 579
Hpy99I CGWCG 1 cut(s) 1059
HpyAV CCTTC 2 cut(s) 541, 947
HpyCH4III ACNGT 4 cut(s) 590, 625, 785, 1055
HpyCH4IV ACGT 2 cut(s) 115, 744
HpyCH4V TGCA 3 cut(s) 460, 632, 829
HpyF10VI GCNNNNNNNGC 2 cut(s) 638, 995
HpyF3I CTNAG 1 cut(s) 153
HpySE526I ACGT 2 cut(s) 115, 744
Hsp92II CATG 7 cut(s) 71, 365, 464, 728, 859, 919, 1123
KpnI GGTACC 2 cut(s) 284, 759
Ksp22I TGATCA 1 cut(s) 858
Kzo9I GATC 9 cut(s) 134, 202, 496, 736, 766, 775, 819, 858, 871
LguI GCTCTTC 1 cut(s) 697
LmnI GCTCC 3 cut(s) 377, 409, 449
Lsp1109I GCAGC 2 cut(s) 49, 719
LweI GCATC 2 cut(s) 447, 792
MabI ACCWGGT 1 cut(s) 478
MaeI CTAG 2 cut(s) 9, 849
MaeII ACGT 2 cut(s) 115, 744
MaeIII GTNAC 3 cut(s) 625, 779, 1135
MalI GATC 9 cut(s) 136, 204, 498, 738, 768, 777, 821, 860, 873
MbiI CCGCTC 1 cut(s) 412
MboI GATC 9 cut(s) 134, 202, 496, 736, 766, 775, 819, 858, 871
MboII GAAGA 7 cut(s) 173, 240, 581, 714, 806, 809, 966
MflI RGATCY 2 cut(s) 496, 736
MhlI GDGCHC 2 cut(s) 295, 1072
MlyI GAGTC 2 cut(s) 450, 1089
MmeI TCCRAC 1 cut(s) 405
Mph1103I ATGCAT 1 cut(s) 462
MseI TTAA 5 cut(s) 161, 353, 383, 671, 837
MslI CAYNNNNRTG 1 cut(s) 895
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 2 cut(s) 582, 1074
MspR9I CCNGG 4 cut(s) 454, 480, 681, 1074
MvaI CCWGG 3 cut(s) 454, 480, 681
MvnI CGCG 1 cut(s) 700
MwoI GCNNNNNNNGC 2 cut(s) 638, 995
NciI CCSGG 1 cut(s) 1074
NcoI CCATGG 1 cut(s) 915
NdeII GATC 9 cut(s) 134, 202, 496, 736, 766, 775, 819, 858, 871
NlaIII CATG 7 cut(s) 71, 365, 464, 728, 859, 919, 1123
NlaIV GGNNCC 4 cut(s) 123, 282, 451, 757
NmuCI GTSAC 2 cut(s) 625, 1135
NruI TCGCGA 1 cut(s) 700
NsiI ATGCAT 1 cut(s) 462
NspI RCATGY 2 cut(s) 464, 1123
NspV TTCGAA 2 cut(s) 38, 939
PaeI GCATGC 1 cut(s) 464
PagI TCATGA 1 cut(s) 724
PciSI GCTCTTC 1 cut(s) 697
PcsI WCGNNNNNNNCGW 2 cut(s) 775, 1051
PfeI GAWTC 5 cut(s) 527, 654, 695, 920, 1100
PflFI GACNNNGTC 1 cut(s) 810
PflMI CCANNNNNTGG 1 cut(s) 131
PkrI GCNGC 3 cut(s) 64, 709, 854
PleI GAGTC 2 cut(s) 449, 1089
PpsI GAGTC 2 cut(s) 449, 1089
PshBI ATTAAT 1 cut(s) 161
Psp124BI GAGCTC 1 cut(s) 295
Psp6I CCWGG 3 cut(s) 452, 478, 679
PspEI GGTNACC 1 cut(s) 1135
PspFI CCCAGC 1 cut(s) 690
PspGI CCWGG 3 cut(s) 452, 478, 679
PspN4I GGNNCC 4 cut(s) 123, 282, 451, 757
PspPI GGNCC 3 cut(s) 47, 229, 677
PsuI RGATCY 2 cut(s) 496, 736
PsyI GACNNNGTC 1 cut(s) 810
PvuII CAGCTG 1 cut(s) 254
RruI TCGCGA 1 cut(s) 700
RsaI GTAC 7 cut(s) 170, 282, 622, 747, 757, 966, 1150
RsaNI GTAC 7 cut(s) 169, 281, 621, 746, 756, 965, 1149
RseI CAYNNNNRTG 1 cut(s) 895
SacI GAGCTC 1 cut(s) 295
SapI GCTCTTC 1 cut(s) 697
SaqAI TTAA 5 cut(s) 161, 353, 383, 671, 837
SatI GCNGC 3 cut(s) 63, 708, 853
Sau3AI GATC 9 cut(s) 134, 202, 496, 736, 766, 775, 819, 858, 871
Sau96I GGNCC 3 cut(s) 47, 229, 677
SchI GAGTC 2 cut(s) 450, 1089
ScrFI CCNGG 4 cut(s) 454, 480, 681, 1074
SduI GDGCHC 2 cut(s) 295, 1072
SexAI ACCWGGT 1 cut(s) 478
SfaNI GCATC 2 cut(s) 447, 792
SfcI CTRYAG 1 cut(s) 255
SfuI TTCGAA 2 cut(s) 38, 939
SinI GGWCC 2 cut(s) 47, 677
SmiMI CAYNNNNRTG 1 cut(s) 895
SmlI CTYRAG 1 cut(s) 1152
SmoI CTYRAG 1 cut(s) 1152
SphI GCATGC 1 cut(s) 464
SsiI CCGC 4 cut(s) 119, 412, 852, 987
SspMI CTAG 2 cut(s) 9, 849
SstI GAGCTC 1 cut(s) 295
StyD4I CCNGG 4 cut(s) 452, 478, 679, 1072
StyI CCWWGG 1 cut(s) 915
TaaI ACNGT 4 cut(s) 590, 625, 785, 1055
TaiI ACGT 2 cut(s) 118, 747
TaqI TCGA 8 cut(s) 38, 328, 517, 558, 795, 901, 939, 1098
TatI WGTACW 1 cut(s) 168
TauI GCSGC 1 cut(s) 855
TfiI GAWTC 5 cut(s) 527, 654, 695, 920, 1100
Tru1I TTAA 5 cut(s) 161, 353, 383, 671, 837
Tru9I TTAA 5 cut(s) 161, 353, 383, 671, 837
TscAI CASTG 2 cut(s) 634, 664
TseFI GTSAC 2 cut(s) 625, 1135
TseI GCWGC 2 cut(s) 62, 707
Tsp45I GTSAC 2 cut(s) 625, 1135
TspDTI ATGAA 6 cut(s) 110, 450, 460, 576, 713, 920
TspGWI ACGGA 5 cut(s) 114, 305, 378, 779, 952
TspRI CASTG 2 cut(s) 634, 664
Tth111I GACNNNGTC 1 cut(s) 810
Van91I CCANNNNNTGG 1 cut(s) 131
VpaK11BI GGWCC 2 cut(s) 47, 677
VspI ATTAAT 1 cut(s) 161
XapI RAATTY 5 cut(s) 28, 40, 330, 428, 1010
XceI RCATGY 2 cut(s) 464, 1123
XcmI CCANNNNNNNNNTGG 1 cut(s) 687
XspI CTAG 2 cut(s) 9, 849
Zsp2I ATGCAT 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.