RLG00000000376

Respiratory-chain NADH dehydrogenase, 49 Kd subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006041
Physical Location & Seq
Reverse (-)
768956 .. 769915
960 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000376

Sequence Viewer

Length: 960 bp
ATGGCCCAAGAACACGCTCATTCTTCAGCCGTAGAGAGACTTTTGAATTGCGAGGTACCATTACGAGCTCAATATATACGAGTGTTATTCCGTGAAATAACTCGAATTTCAAATCATTCACTTGCTTTAACTACTCATGCTATGGATGTGGGAGCATTAACTCCGTTTCTGTGGGCTTTTGAGGAGCGGGAGAAATTGTTGGAATTCTATGAAAGAGTCTCAGGAGCCAGGATGCATGCCAGTTTCATACGACCAGGTGGAGTGGCACAAGATCTGCCTCTTGGCTTATGTCGAGATATTGATTCTTTTACACAACAATTTGCTTCTCGTATCGACGAATTAGAAGAGATGTTAACCGGCAACCGTATCTGGAAACAACGATTAGTGGATATTGGTACTGTCACTGCACAGCAAGCAAAGGATTGGGGATTCAGTGGTGTAATGTTAAGAGGTTCAGGGGTATGCTGGGATTTGCGAAGAGCAGCACCTTACGATGTTTATGACCAGTTGGATTTTGACGTACCAGTAGGTACCAGAGGAGATTGCTATGATCGTTACTGTATTCGTATTGAAGAGATGCGACAAAGTGTTCGGATCATTGTGCAATGTCTTAATAAAATGCCTAGTGGCATGATCAAAGCTGATGATCGTAAACTATGTCCTCCATCACGAGGTCGAATGAAACTATCCATGGAATCCTTAATTCACCATTTCGAACTTTATACAGAAGGTTTTTCCGTACCAGCTTCTTCTACCTATACCGCAGTTGAAGCACCTAAAGGAGAATTTGGTGTCTTTCTGGTCAGTAATGGAAGCAATCGTCCTTACCGTTGTAAAATAAGAGCACCTGGCTTTGCCCATTTACAAGGACTCGATTTTATGTCCAAACATCACATGCTAGCGGATGTGGTCACCATCATAGGTACTCAAGATATTGTGTCTGGAGAGGTGGATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Complex1_49kDa

Protein Analysis

320

Amino Acids

36.13

Weight (kDa)

6.56

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Complex1_49kDa PF00346 49 - 319 9.7e-122 Respiratory-chain NADH dehydrogenase, 49 Kd subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 55, 530
AccB1I GGYRCC 2 cut(s) 55, 530
AccBSI CCGCTC 1 cut(s) 187
AciI CCGC 3 cut(s) 187, 762, 902
AclWI GGATC 1 cut(s) 602
AcsI RAATTY 3 cut(s) 105, 203, 785
AcuI CTGAAG 1 cut(s) 9
AfaI GTAC 6 cut(s) 57, 397, 522, 532, 741, 925
AfiI CCNNNNNNNGG 1 cut(s) 671
AgsI TTSAA 4 cut(s) 46, 111, 572, 770
AjnI CCWGG 3 cut(s) 227, 253, 847
AluBI AGCT 3 cut(s) 68, 641, 746
AluI AGCT 3 cut(s) 68, 641, 746
Alw21I GWGCWC 2 cut(s) 70, 847
Alw26I GTCTC 2 cut(s) 31, 223
AlwI GGATC 1 cut(s) 602
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 482
ApoI RAATTY 3 cut(s) 105, 203, 785
ArsI GACNNNNNNTTYG 2 cut(s) 573, 605
Asp718I GGTACC 2 cut(s) 55, 530
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 2 cut(s) 698, 904
AsuII TTCGAA 1 cut(s) 714
AsuNHI GCTAGC 1 cut(s) 898
BanI GGYRCC 2 cut(s) 55, 530
BanII GRGCYC 1 cut(s) 70
BauI CACGAG 1 cut(s) 669
Bbv12I GWGCWC 2 cut(s) 70, 847
BbvI GCAGC 1 cut(s) 494
BccI CCATC 2 cut(s) 673, 923
BceAI ACGGC 1 cut(s) 14
BciT130I CCWGG 3 cut(s) 229, 255, 849
BclI TGATCA 1 cut(s) 633
BcoDI GTCTC 2 cut(s) 31, 223
BfaI CTAG 2 cut(s) 624, 899
BglII AGATCT 1 cut(s) 271
BisI GCNGC 1 cut(s) 483
BlsI GCNGC 1 cut(s) 484
Bme1390I CCNGG 3 cut(s) 229, 255, 849
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 3 cut(s) 57, 226, 532
BmrFI CCNGG 3 cut(s) 229, 255, 849
BmsI GCATC 2 cut(s) 222, 567
BmtI GCTAGC 1 cut(s) 902
Bpu14I TTCGAA 1 cut(s) 714
BpuEI CTTGAG 1 cut(s) 912
BsaJI CCNNGG 1 cut(s) 690
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 1 cut(s) 671
Bse118I RCCGGY 1 cut(s) 356
Bse1I ACTGG 3 cut(s) 240, 505, 524
Bse3DI GCAATG 1 cut(s) 611
BseBI CCWGG 3 cut(s) 229, 255, 849
BseDI CCNNGG 1 cut(s) 690
BseGI GGATG 3 cut(s) 151, 237, 910
BseLI CCNNNNNNNGG 1 cut(s) 671
BseMI GCAATG 1 cut(s) 611
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 3 cut(s) 240, 505, 524
BseRI GAGGAG 2 cut(s) 197, 552
BseXI GCAGC 1 cut(s) 494
BseYI CCCAGC 1 cut(s) 465
BsgI GTGCAG 1 cut(s) 390
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 2 cut(s) 55, 530
BsiHKAI GWGCWC 2 cut(s) 70, 847
BsiSI CCGG 1 cut(s) 357
BslI CCNNNNNNNGG 1 cut(s) 671
BsmAI GTCTC 2 cut(s) 31, 223
BsnI GGCC 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 714
Bsp1286I GDGCHC 2 cut(s) 70, 847
Bsp143I GATC 5 cut(s) 271, 550, 594, 633, 646
Bsp19I CCATGG 1 cut(s) 690
BspACI CCGC 3 cut(s) 187, 762, 902
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 233
BspLI GGNNCC 3 cut(s) 57, 226, 532
BspOI GCTAGC 1 cut(s) 902
BspPI GGATC 1 cut(s) 602
BspQI GCTCTTC 1 cut(s) 472
BspT104I TTCGAA 1 cut(s) 714
BspT107I GGYRCC 2 cut(s) 55, 530
BsrBI CCGCTC 1 cut(s) 187
BsrDI GCAATG 1 cut(s) 611
BsrFI RCCGGY 1 cut(s) 356
BsrI ACTGG 3 cut(s) 240, 505, 524
BssAI RCCGGY 1 cut(s) 356
BssECI CCNNGG 1 cut(s) 690
BssMI GATC 5 cut(s) 271, 550, 594, 633, 646
BssSI CACGAG 1 cut(s) 669
BssT1I CCWWGG 1 cut(s) 690
Bst2BI CACGAG 1 cut(s) 669
Bst2UI CCWGG 3 cut(s) 229, 255, 849
Bst4CI ACNGT 4 cut(s) 365, 400, 560, 830
Bst6I CTCTTC 3 cut(s) 339, 472, 567
BstBI TTCGAA 1 cut(s) 714
BstC8I GCNNGC 3 cut(s) 237, 414, 900
BstDEI CTNAG 1 cut(s) 220
BstDSI CCRYGG 1 cut(s) 690
BstEII GGTNACC 1 cut(s) 910
BstF5I GGATG 3 cut(s) 151, 237, 910
BstKTI GATC 5 cut(s) 274, 553, 597, 636, 649
BstMAI GTCTC 2 cut(s) 31, 223
BstMBI GATC 5 cut(s) 271, 550, 594, 633, 646
BstMWI GCNNNNNNNGC 2 cut(s) 413, 770
BstNI CCWGG 3 cut(s) 229, 255, 849
BstNSI RCATGY 2 cut(s) 239, 898
BstPI GGTNACC 1 cut(s) 910
BstSCI CCNGG 3 cut(s) 227, 253, 847
BstV1I GCAGC 1 cut(s) 494
BstX2I RGATCY 1 cut(s) 271
BstYI RGATCY 1 cut(s) 271
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 690
BtsCI GGATG 3 cut(s) 151, 237, 910
BtsI GCAGTG 1 cut(s) 402
BtsIMutI CAGTG 2 cut(s) 402, 439
Cac8I GCNNGC 3 cut(s) 237, 414, 900
Cfr10I RCCGGY 1 cut(s) 356
Cfr13I GGNCC 1 cut(s) 4
CsiI ACCWGGT 1 cut(s) 253
Csp6I GTAC 6 cut(s) 56, 396, 521, 531, 740, 924
CviAII CATG 5 cut(s) 137, 236, 631, 691, 895
CviJI RGCY 9 cut(s) 5, 29, 68, 176, 227, 285, 641, 746, 852
CviKI_1 RGCY 9 cut(s) 5, 29, 68, 176, 227, 285, 641, 746, 852
CviQI GTAC 6 cut(s) 56, 396, 521, 531, 740, 924
DdeI CTNAG 1 cut(s) 220
DpnI GATC 5 cut(s) 273, 552, 596, 635, 648
DpnII GATC 5 cut(s) 271, 550, 594, 633, 646
Eam1104I CTCTTC 3 cut(s) 339, 472, 567
EarI CTCTTC 3 cut(s) 339, 472, 567
Ecl136II GAGCTC 1 cut(s) 68
Eco130I CCWWGG 1 cut(s) 690
Eco24I GRGCYC 1 cut(s) 70
Eco53kI GAGCTC 1 cut(s) 68
Eco57I CTGAAG 1 cut(s) 9
Eco91I GGTNACC 1 cut(s) 910
EcoICRI GAGCTC 1 cut(s) 68
EcoO65I GGTNACC 1 cut(s) 910
EcoRI GAATTC 1 cut(s) 203
EcoRII CCWGG 3 cut(s) 227, 253, 847
EcoT14I CCWWGG 1 cut(s) 690
EcoT22I ATGCAT 1 cut(s) 237
EcoT38I GRGCYC 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 690
FaeI CATG 5 cut(s) 140, 239, 634, 694, 898
FatI CATG 5 cut(s) 136, 235, 630, 690, 894
FauI CCCGC 1 cut(s) 180
FbaI TGATCA 1 cut(s) 633
Fnu4HI GCNGC 1 cut(s) 483
FokI GGATG 3 cut(s) 158, 244, 917
FriOI GRGCYC 1 cut(s) 70
Fsp4HI GCNGC 1 cut(s) 483
FspBI CTAG 2 cut(s) 624, 899
GluI GCNGC 1 cut(s) 483
GsaI CCCAGC 1 cut(s) 469
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 357
Hin1II CATG 5 cut(s) 140, 239, 634, 694, 898
HincII GTYRAC 1 cut(s) 354
HindII GTYRAC 1 cut(s) 354
HinfI GANTC 5 cut(s) 216, 302, 429, 695, 870
HpaI GTTAAC 1 cut(s) 354
HpaII CCGG 1 cut(s) 357
HphI GGTGA 2 cut(s) 698, 904
Hpy166II GTNNAC 2 cut(s) 354, 653
Hpy188I TCNGA 1 cut(s) 594
Hpy188III TCNNGA 6 cut(s) 222, 293, 370, 669, 929, 942
Hpy8I GTNNAC 2 cut(s) 354, 653
Hpy99I CGWCG 1 cut(s) 338
HpyAV CCTTC 1 cut(s) 722
HpyCH4III ACNGT 4 cut(s) 365, 400, 560, 830
HpyCH4IV ACGT 1 cut(s) 519
HpyCH4V TGCA 3 cut(s) 235, 407, 604
HpyF10VI GCNNNNNNNGC 2 cut(s) 413, 770
HpyF3I CTNAG 1 cut(s) 220
HpySE526I ACGT 1 cut(s) 519
Hsp92II CATG 5 cut(s) 140, 239, 634, 694, 898
KpnI GGTACC 2 cut(s) 59, 534
Ksp22I TGATCA 1 cut(s) 633
KspAI GTTAAC 1 cut(s) 354
Kzo9I GATC 5 cut(s) 271, 550, 594, 633, 646
LguI GCTCTTC 1 cut(s) 472
LmnI GCTCC 3 cut(s) 152, 184, 224
Lsp1109I GCAGC 1 cut(s) 494
LweI GCATC 2 cut(s) 222, 567
MabI ACCWGGT 1 cut(s) 253
MaeI CTAG 2 cut(s) 624, 899
MaeII ACGT 1 cut(s) 519
MaeIII GTNAC 3 cut(s) 400, 554, 910
MalI GATC 5 cut(s) 273, 552, 596, 635, 648
MbiI CCGCTC 1 cut(s) 187
MboI GATC 5 cut(s) 271, 550, 594, 633, 646
MboII GAAGA 5 cut(s) 15, 356, 489, 584, 741
MflI RGATCY 1 cut(s) 271
MhlI GDGCHC 2 cut(s) 70, 847
MluCI AATT 8 cut(s) 46, 105, 194, 203, 317, 338, 702, 785
MlyI GAGTC 2 cut(s) 225, 864
MmeI TCCRAC 2 cut(s) 180, 489
MnlI CCTC 8 cut(s) 46, 175, 288, 443, 530, 665, 672, 940
Mph1103I ATGCAT 1 cut(s) 237
MseI TTAA 6 cut(s) 128, 158, 353, 446, 612, 701
MspI CCGG 1 cut(s) 357
MspR9I CCNGG 3 cut(s) 229, 255, 849
MvaI CCWGG 3 cut(s) 229, 255, 849
MwoI GCNNNNNNNGC 2 cut(s) 413, 770
NcoI CCATGG 1 cut(s) 690
NdeII GATC 5 cut(s) 271, 550, 594, 633, 646
NheI GCTAGC 1 cut(s) 898
NlaIII CATG 5 cut(s) 140, 239, 634, 694, 898
NlaIV GGNNCC 3 cut(s) 57, 226, 532
NmuCI GTSAC 2 cut(s) 400, 910
NsiI ATGCAT 1 cut(s) 237
NspI RCATGY 2 cut(s) 239, 898
NspV TTCGAA 1 cut(s) 714
PaeI GCATGC 1 cut(s) 239
PciSI GCTCTTC 1 cut(s) 472
PcsI WCGNNNNNNNCGW 1 cut(s) 826
PfeI GAWTC 3 cut(s) 302, 429, 695
PkrI GCNGC 1 cut(s) 484
PleI GAGTC 2 cut(s) 224, 864
PpsI GAGTC 2 cut(s) 224, 864
Psp124BI GAGCTC 1 cut(s) 70
Psp6I CCWGG 3 cut(s) 227, 253, 847
PspEI GGTNACC 1 cut(s) 910
PspFI CCCAGC 1 cut(s) 465
PspGI CCWGG 3 cut(s) 227, 253, 847
PspN4I GGNNCC 3 cut(s) 57, 226, 532
PspPI GGNCC 1 cut(s) 4
PsuI RGATCY 1 cut(s) 271
RsaI GTAC 6 cut(s) 57, 397, 522, 532, 741, 925
RsaNI GTAC 6 cut(s) 56, 396, 521, 531, 740, 924
SacI GAGCTC 1 cut(s) 70
SapI GCTCTTC 1 cut(s) 472
SaqAI TTAA 6 cut(s) 128, 158, 353, 446, 612, 701
SatI GCNGC 1 cut(s) 483
Sau3AI GATC 5 cut(s) 271, 550, 594, 633, 646
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 2 cut(s) 225, 864
ScrFI CCNGG 3 cut(s) 229, 255, 849
SduI GDGCHC 2 cut(s) 70, 847
SexAI ACCWGGT 1 cut(s) 253
SfaNI GCATC 2 cut(s) 222, 567
SfuI TTCGAA 1 cut(s) 714
SmlI CTYRAG 1 cut(s) 927
SmoI CTYRAG 1 cut(s) 927
SphI GCATGC 1 cut(s) 239
Sse9I AATT 8 cut(s) 46, 105, 194, 203, 317, 338, 702, 785
SsiI CCGC 3 cut(s) 187, 762, 902
SspMI CTAG 2 cut(s) 624, 899
SstI GAGCTC 1 cut(s) 70
StyD4I CCNGG 3 cut(s) 227, 253, 847
StyI CCWWGG 1 cut(s) 690
TaaI ACNGT 4 cut(s) 365, 400, 560, 830
TaiI ACGT 1 cut(s) 522
TaqI TCGA 6 cut(s) 103, 292, 333, 676, 714, 873
TasI AATT 8 cut(s) 46, 105, 194, 203, 317, 338, 702, 785
TfiI GAWTC 3 cut(s) 302, 429, 695
Tru1I TTAA 6 cut(s) 128, 158, 353, 446, 612, 701
Tru9I TTAA 6 cut(s) 128, 158, 353, 446, 612, 701
TscAI CASTG 2 cut(s) 409, 439
TseFI GTSAC 2 cut(s) 400, 910
TseI GCWGC 1 cut(s) 482
Tsp45I GTSAC 2 cut(s) 400, 910
TspDTI ATGAA 3 cut(s) 225, 235, 695
TspGWI ACGGA 3 cut(s) 80, 153, 727
TspRI CASTG 2 cut(s) 409, 439
XapI RAATTY 3 cut(s) 105, 203, 785
XceI RCATGY 2 cut(s) 239, 898
XspI CTAG 2 cut(s) 624, 899
Zsp2I ATGCAT 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.