RorugMtG0001100

Respiratory-chain NADH dehydrogenase, 49 Kd subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000009
OG0006041
Physical Location & Seq
Forward (+)
67513 .. 78386
10874 bp
Loading structure...
UTR
Exon/CDS
Intron
RorugMtG0001100.1

Sequence Viewer

Length: 1740 bp
ATGTCAATATATGAATTGTTTCATTATTCGTTATTTCCGGGTCTTTTCATTGCATTCACTTACAACAAGAAACAACCACCTGCGTTTGGTGCAGCACCTGCATTTTGGTGCATTCTTCTTTCTTTCCTTGGTCTTTCGTTCCGTCATATTCCTAATAACTTATCCAATTACAACGTATTAACCGCTAATGCACCTTTCTTTTATCAAATCTCAGGGACATGGTCTAATCATGAGGGTAGTATTTTATCATGGTGTCGGATCCCAAGTTTTTATGGATTCCTTCTTTGTTACCGGGGTCGACCCCAAAGCCATAATGTCTCAAAACGAGGAGGCCATAGAGAAACTTTTTTTTATTCCTTTGTCTCGAACTTCGTGAAGAACTCCATTCTATCTCTCCCTCGTTACGAAGAAAAAAGTGGTGGTGTTGTACACCGCCAGTTGTACACTCCCTTCGTTCTACGAACCCTTGTTGATTCTGAACTTCCTTCGCGAAGGAACCGGACTTTTGACGGGCTAGCCCTTTTTTATGCGCCGCTTTACCCTGAAAGGAAAAAGAGTTTTGCTCCTCTGGGCGCTAGGCGCTCCCGTGCTTCGCGAGAAGGAAAAAGGATTCATCCTTTGTTACATCTGGCACGAGATGATAAAGAGAGAGCTTCGTCTATCGATGAACAGCGGATTGACGGAGCTCTTGGCATTGCTTTGTTTTTCTCTCCTTTCCTATCAGCGAGTCCCGATCCTTTTGTTCGAAATTTCTTCGTTCGTACCGAACCGCTTGCAGAATCAAATCCTGTTTCACAAGATCCTATATCAGCTATACATCCTCCTTGCATTTATGCCGGAGACGTCGCCAGTGCTATGGGCTTTGGCTTATGTAGATCAAAAATGATGAATGGGATTGTGGCACTCCACTCGCCGCCAATGCGGAAGGATGCCGCCGAAAAGAATGGAACGCTGCTTCGCTCTGCTGGATGCGTCGGATCCCGTACAACAAGCGAGCTTTTTACCCTCAAATTCAAACATGTGGGCGAAAAATGCTATCCTGCTCTATTGTTGCGTAGCAATAGAAGCCTGCTCATGCTGCTTCGGCGGCGCTTTTTCGCCTTCTCTTCGCTCTGGGCAGGAGCGCTAGTGGAGACGGGGAGGGAGCAGGCGAGGCGTGTCGTTCGTAATGGACAGAAAGATACCACCACTTCGCCTCTTTGTTGGACCGCCGGCGCGAACACAGTGGTCTCTGACCAAGACCAGGAACCAATTCGAATTTGGATCTTGACATGTCGGTGGTTTTTAACCGTAGGCATCTTGCCAGGAAGTTGGTGGGCTCATCATGAATTAGGTCGGGGTGGCTGGTGGTTTCGGGATCCCGTAGAAAATGCTTCTTTTATGCCTCGGGTATTAGCCACAGCTCGTATTCATTCAGTAATTCTACCCCTTCTTCATTCTTGGACCTCGTTTCTTAATATTGTGACTCTTCCATGCTGTGTCTCAGGAACCTTTTCAATACGGTCCGGATTGCTAGCTTCCGTTCATAGTTTTGCTACAGATGATACACGAGGGATCTTTTTATGGCGGTTCTTCCTTCTAATGACCGGCATATCTATGATTCTTTTCTCCCAGATGAAGCAGCAGGCATCGGTCCGTAGAACCTATAAAAAAGAGATGGTTGTGGCGCGAAGTACTCTTGTGCACCTACGTCACGCGGCTCGCGCGAAACCCCGCCCCGTTATGTTATGGAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Complex1_49kDa

Protein Analysis

579

Amino Acids

65.62

Weight (kDa)

10.23

Isoelectric Point (pI)

52.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_C_asm PF01578 236 - 503 3e-16 Cytochrome C assembly protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 88, 106
AatII GACGTC 1 cut(s) 846
Acc36I ACCTGC 2 cut(s) 88, 106
AccI GTMKAC 1 cut(s) 298
AccII CGCG 7 cut(s) 490, 595, 1217, 1669, 1697, 1704, 1706
AccIII TCCGGA 1 cut(s) 1505
AcsI RAATTY 3 cut(s) 748, 1010, 1257
AcyI GRCGYC 1 cut(s) 843
AfaI GTAC 5 cut(s) 429, 443, 763, 985, 1675
AfeI AGCGCT 1 cut(s) 1125
AfiI CCNNNNNNNGG 2 cut(s) 86, 1243
AflIII ACRYGT 2 cut(s) 1018, 1271
AgsI TTSAA 2 cut(s) 1015, 1497
AjnI CCWGG 2 cut(s) 1242, 1303
AluBI AGCT 6 cut(s) 653, 686, 812, 997, 1403, 1517
AluI AGCT 6 cut(s) 653, 686, 812, 997, 1403, 1517
Alw21I GWGCWC 2 cut(s) 688, 1686
Alw26I GTCTC 6 cut(s) 322, 367, 834, 1127, 1234, 1486
Alw44I GTGCAC 1 cut(s) 1682
AlwNI CAGNNNCTG 1 cut(s) 98
Ama87I CYCGRG 1 cut(s) 1386
Aor13HI TCCGGA 1 cut(s) 1505
Aor51HI AGCGCT 1 cut(s) 1125
AoxI GGCC 1 cut(s) 331
ApaLI GTGCAC 1 cut(s) 1682
ApeKI GCWGC 4 cut(s) 92, 952, 1078, 1621
ApoI RAATTY 3 cut(s) 748, 1010, 1257
Asp700I GAANNNNTTC 3 cut(s) 18, 1251, 1492
AspLEI GCGC 8 cut(s) 532, 575, 582, 1092, 1126, 1217, 1669, 1706
AspS9I GGNCC 4 cut(s) 1206, 1443, 1503, 1633
AsuC2I CCSGG 2 cut(s) 39, 293
AsuII TTCGAA 2 cut(s) 745, 1255
AsuNHI GCTAGC 2 cut(s) 514, 1513
AvaI CYCGRG 1 cut(s) 1386
AvaII GGWCC 4 cut(s) 1206, 1443, 1503, 1633
BaeGI GKGCMC 1 cut(s) 1686
BamHI GGATCC 3 cut(s) 258, 977, 1357
BanII GRGCYC 2 cut(s) 688, 1321
BarI GAAGNNNNNNTAC 2 cut(s) 434, 466
BauI CACGAG 2 cut(s) 633, 1548
Bbv12I GWGCWC 2 cut(s) 688, 1686
BbvI GCAGC 4 cut(s) 104, 939, 1065, 1633
BccI CCATC 1 cut(s) 1651
BcgI CGANNNNNNTGC 4 cut(s) 755, 789, 891, 925
BciT130I CCWGG 2 cut(s) 1244, 1305
BcnI CCSGG 2 cut(s) 39, 293
BcoDI GTCTC 6 cut(s) 322, 367, 834, 1127, 1234, 1486
BfaI CTAG 4 cut(s) 515, 576, 1127, 1514
BfmI CTRYAG 1 cut(s) 1536
BfoI RGCGCY 4 cut(s) 576, 583, 1093, 1127
BfuAI ACCTGC 2 cut(s) 88, 106
BisI GCNGC 9 cut(s) 93, 533, 914, 933, 953, 1079, 1088, 1622, 1698
BlsI GCNGC 9 cut(s) 94, 534, 915, 934, 954, 1080, 1089, 1623, 1699
BmcAI AGTACT 1 cut(s) 1675
Bme1390I CCNGG 4 cut(s) 39, 293, 1244, 1305
Bme18I GGWCC 4 cut(s) 1206, 1443, 1503, 1633
BmeT110I CYCGRG 1 cut(s) 1386
BmgT120I GGNCC 4 cut(s) 1206, 1443, 1503, 1633
BmiI GGNNCC 6 cut(s) 260, 497, 979, 1248, 1359, 1489
BmrFI CCNGG 4 cut(s) 39, 293, 1244, 1305
BmsI GCATC 4 cut(s) 919, 959, 1305, 1637
BmtI GCTAGC 2 cut(s) 518, 1517
BplI GAGNNNNNCTC 2 cut(s) 547, 579
Bpu14I TTCGAA 2 cut(s) 745, 1255
BpuMI CCSGG 2 cut(s) 39, 293
Bsa29I ATCGAT 1 cut(s) 663
BsaHI GRCGYC 1 cut(s) 843
BsaI GGTCTC 1 cut(s) 1234
BsaJI CCNNGG 3 cut(s) 127, 292, 1385
BsaWI WCCGGW 2 cut(s) 498, 1505
BsaXI ACNNNNNCTCC 2 cut(s) 1113, 1143
Bsc4I CCNNNNNNNGG 2 cut(s) 86, 1243
Bse118I RCCGGY 2 cut(s) 1211, 1586
Bse1I ACTGG 2 cut(s) 436, 849
Bse3DI GCAATG 2 cut(s) 48, 693
BseAI TCCGGA 1 cut(s) 1505
BseBI CCWGG 2 cut(s) 1244, 1305
BseCI ATCGAT 1 cut(s) 663
BseDI CCNNGG 3 cut(s) 127, 292, 1385
BseGI GGATG 4 cut(s) 613, 817, 934, 974
BseLI CCNNNNNNNGG 2 cut(s) 86, 1243
BseMI GCAATG 2 cut(s) 48, 693
BseMII CTCAG 2 cut(s) 225, 1497
BseNI ACTGG 2 cut(s) 436, 849
BseRI GAGGAG 2 cut(s) 342, 555
BseSI GKGCMC 1 cut(s) 1686
BseXI GCAGC 4 cut(s) 104, 939, 1065, 1633
BsgI GTGCAG 1 cut(s) 111
Bsh1236I CGCG 7 cut(s) 490, 595, 1217, 1669, 1697, 1704, 1706
BshFI GGCC 1 cut(s) 333
BshVI ATCGAT 1 cut(s) 663
BsiHKAI GWGCWC 2 cut(s) 688, 1686
BsiHKCI CYCGRG 1 cut(s) 1386
BsiSI CCGG 7 cut(s) 38, 292, 499, 837, 1212, 1506, 1587
BslFI GGGAC 2 cut(s) 229, 714
BslI CCNNNNNNNGG 2 cut(s) 86, 1243
BsmAI GTCTC 6 cut(s) 322, 367, 834, 1127, 1234, 1486
BsmBI CGTCTC 2 cut(s) 834, 1127
BsmFI GGGAC 2 cut(s) 229, 714
BsmI GAATGC 2 cut(s) 53, 111
BsnI GGCC 1 cut(s) 333
Bso31I GGTCTC 1 cut(s) 1234
BsoBI CYCGRG 1 cut(s) 1386
Bsp119I TTCGAA 2 cut(s) 745, 1255
Bsp1286I GDGCHC 3 cut(s) 688, 1321, 1686
Bsp13I TCCGGA 1 cut(s) 1505
Bsp1407I TGTACA 2 cut(s) 427, 441
Bsp143I GATC 8 cut(s) 258, 733, 799, 875, 977, 1263, 1357, 1554
Bsp68I TCGCGA 2 cut(s) 490, 595
BspANI GGCC 1 cut(s) 333
BspCNI CTCAG 2 cut(s) 224, 1496
BspDI ATCGAT 1 cut(s) 663
BspEI TCCGGA 1 cut(s) 1505
BspFNI CGCG 7 cut(s) 490, 595, 1217, 1669, 1697, 1704, 1706
BspHI TCATGA 2 cut(s) 229, 1324
BspLI GGNNCC 6 cut(s) 260, 497, 979, 1248, 1359, 1489
BspMI ACCTGC 2 cut(s) 88, 106
BspOI GCTAGC 2 cut(s) 518, 1517
BspT104I TTCGAA 2 cut(s) 745, 1255
BspTNI GGTCTC 1 cut(s) 1234
BsrDI GCAATG 2 cut(s) 48, 693
BsrFI RCCGGY 2 cut(s) 1211, 1586
BsrGI TGTACA 2 cut(s) 427, 441
BsrI ACTGG 2 cut(s) 436, 849
BssAI RCCGGY 2 cut(s) 1211, 1586
BssECI CCNNGG 3 cut(s) 127, 292, 1385
BssMI GATC 8 cut(s) 258, 733, 799, 875, 977, 1263, 1357, 1554
BssNI GRCGYC 1 cut(s) 843
BssSI CACGAG 2 cut(s) 633, 1548
BssT1I CCWWGG 1 cut(s) 127
Bst2BI CACGAG 2 cut(s) 633, 1548
Bst2UI CCWGG 2 cut(s) 1244, 1305
Bst4CI ACNGT 3 cut(s) 1225, 1291, 1503
Bst6I CTCTTC 2 cut(s) 1111, 1473
BstACI GRCGYC 1 cut(s) 843
BstAPI GCANNNNNTGC 1 cut(s) 98
BstAUI TGTACA 2 cut(s) 427, 441
BstBI TTCGAA 2 cut(s) 745, 1255
BstC8I GCNNGC 9 cut(s) 516, 774, 995, 1070, 1149, 1213, 1515, 1626, 1702
BstDEI CTNAG 2 cut(s) 211, 1483
BstF5I GGATG 4 cut(s) 613, 817, 934, 974
BstFNI CGCG 7 cut(s) 490, 595, 1217, 1669, 1697, 1704, 1706
BstH2I RGCGCY 4 cut(s) 576, 583, 1093, 1127
BstHHI GCGC 8 cut(s) 532, 575, 582, 1092, 1126, 1217, 1669, 1706
BstKTI GATC 8 cut(s) 261, 736, 802, 878, 980, 1266, 1360, 1557
BstMAI GTCTC 6 cut(s) 322, 367, 834, 1127, 1234, 1486
BstMBI GATC 8 cut(s) 258, 733, 799, 875, 977, 1263, 1357, 1554
BstNI CCWGG 2 cut(s) 1244, 1305
BstNSI RCATGY 2 cut(s) 1022, 1275
BstSCI CCNGG 4 cut(s) 37, 291, 1242, 1303
BstSFI CTRYAG 1 cut(s) 1536
BstSLI GKGCMC 1 cut(s) 1686
BstUI CGCG 7 cut(s) 490, 595, 1217, 1669, 1697, 1704, 1706
BstV1I GCAGC 4 cut(s) 104, 939, 1065, 1633
BstX2I RGATCY 6 cut(s) 258, 799, 977, 1263, 1357, 1554
BstXI CCANNNNNNTGG 2 cut(s) 856, 1311
BstYI RGATCY 6 cut(s) 258, 799, 977, 1263, 1357, 1554
Bsu15I ATCGAT 1 cut(s) 663
BsuRI GGCC 1 cut(s) 333
BsuTUI ATCGAT 1 cut(s) 663
BtsCI GGATG 4 cut(s) 613, 817, 934, 974
BtsIMutI CAGTG 2 cut(s) 856, 1230
BtuMI TCGCGA 2 cut(s) 490, 595
BveI ACCTGC 2 cut(s) 88, 106
Cac8I GCNNGC 9 cut(s) 516, 774, 995, 1070, 1149, 1213, 1515, 1626, 1702
CaiI CAGNNNCTG 1 cut(s) 98
CciI TCATGA 2 cut(s) 229, 1324
CfoI GCGC 8 cut(s) 532, 575, 582, 1092, 1126, 1217, 1669, 1706
Cfr10I RCCGGY 2 cut(s) 1211, 1586
Cfr13I GGNCC 4 cut(s) 1206, 1443, 1503, 1633
ClaI ATCGAT 1 cut(s) 663
CpoI CGGWCCG 2 cut(s) 1503, 1633
CseI GACGC 1 cut(s) 961
Csp6I GTAC 5 cut(s) 428, 442, 762, 984, 1674
CspI CGGWCCG 2 cut(s) 1503, 1633
CviAII CATG 8 cut(s) 219, 230, 249, 1019, 1075, 1272, 1325, 1473
CviQI GTAC 5 cut(s) 428, 442, 762, 984, 1674
DdeI CTNAG 2 cut(s) 211, 1483
DpnI GATC 8 cut(s) 260, 735, 801, 877, 979, 1265, 1359, 1556
DpnII GATC 8 cut(s) 258, 733, 799, 875, 977, 1263, 1357, 1554
Eam1104I CTCTTC 2 cut(s) 1111, 1473
EarI CTCTTC 2 cut(s) 1111, 1473
Ecl136II GAGCTC 1 cut(s) 686
Eco130I CCWWGG 1 cut(s) 127
Eco24I GRGCYC 2 cut(s) 688, 1321
Eco31I GGTCTC 1 cut(s) 1234
Eco47I GGWCC 4 cut(s) 1206, 1443, 1503, 1633
Eco47III AGCGCT 1 cut(s) 1125
Eco53kI GAGCTC 1 cut(s) 686
Eco88I CYCGRG 1 cut(s) 1386
EcoICRI GAGCTC 1 cut(s) 686
EcoRII CCWGG 2 cut(s) 1242, 1303
EcoT14I CCWWGG 1 cut(s) 127
EcoT38I GRGCYC 2 cut(s) 688, 1321
ErhI CCWWGG 1 cut(s) 127
Esp3I CGTCTC 2 cut(s) 834, 1127
FaeI CATG 8 cut(s) 222, 233, 252, 1022, 1078, 1275, 1328, 1476
FaqI GGGAC 2 cut(s) 229, 714
FatI CATG 8 cut(s) 218, 229, 248, 1018, 1074, 1271, 1324, 1472
FauI CCCGC 1 cut(s) 1721
FblI GTMKAC 1 cut(s) 298
Fnu4HI GCNGC 9 cut(s) 93, 533, 914, 933, 953, 1079, 1088, 1622, 1698
FokI GGATG 4 cut(s) 600, 804, 941, 981
FriOI GRGCYC 2 cut(s) 688, 1321
Fsp4HI GCNGC 9 cut(s) 93, 533, 914, 933, 953, 1079, 1088, 1622, 1698
FspBI CTAG 4 cut(s) 515, 576, 1127, 1514
GlaI GCGC 8 cut(s) 531, 574, 581, 1091, 1125, 1216, 1668, 1705
GluI GCNGC 9 cut(s) 93, 533, 914, 933, 953, 1079, 1088, 1622, 1698
HaeII RGCGCY 4 cut(s) 576, 583, 1093, 1127
HaeIII GGCC 1 cut(s) 333
HapII CCGG 7 cut(s) 38, 292, 499, 837, 1212, 1506, 1587
HgaI GACGC 1 cut(s) 961
HhaI GCGC 8 cut(s) 532, 575, 582, 1092, 1126, 1217, 1669, 1706
Hin1I GRCGYC 1 cut(s) 843
Hin1II CATG 8 cut(s) 222, 233, 252, 1022, 1078, 1275, 1328, 1476
Hin6I GCGC 8 cut(s) 530, 573, 580, 1090, 1124, 1215, 1667, 1704
HinP1I GCGC 8 cut(s) 530, 573, 580, 1090, 1124, 1215, 1667, 1704
HincII GTYRAC 1 cut(s) 299
HindII GTYRAC 1 cut(s) 299
HinfI GANTC 7 cut(s) 276, 473, 610, 727, 779, 1465, 1600
HpaII CCGG 7 cut(s) 38, 292, 499, 837, 1212, 1506, 1587
Hpy166II GTNNAC 4 cut(s) 299, 430, 444, 1684
Hpy188I TCNGA 4 cut(s) 258, 478, 977, 1234
Hpy8I GTNNAC 4 cut(s) 299, 430, 444, 1684
Hpy99I CGWCG 2 cut(s) 848, 977
HpyAV CCTTC 9 cut(s) 290, 460, 486, 495, 593, 919, 1111, 1439, 1586
HpyCH4III ACNGT 3 cut(s) 1225, 1291, 1503
HpyCH4IV ACGT 3 cut(s) 174, 843, 1690
HpyCH4V TGCA 8 cut(s) 53, 92, 101, 111, 191, 776, 828, 1684
HpyF3I CTNAG 2 cut(s) 211, 1483
HpySE526I ACGT 3 cut(s) 174, 843, 1690
Hsp92I GRCGYC 1 cut(s) 843
Hsp92II CATG 8 cut(s) 222, 233, 252, 1022, 1078, 1275, 1328, 1476
HspAI GCGC 8 cut(s) 530, 573, 580, 1090, 1124, 1215, 1667, 1704
Kpn2I TCCGGA 1 cut(s) 1505
KroI GCCGGC 1 cut(s) 1211
KroNI GCCGGC 1 cut(s) 1213
Kzo9I GATC 8 cut(s) 258, 733, 799, 875, 977, 1263, 1357, 1554
LmnI GCTCC 5 cut(s) 568, 587, 683, 1121, 1144
Lsp1109I GCAGC 4 cut(s) 104, 939, 1065, 1633
LweI GCATC 4 cut(s) 919, 959, 1305, 1637
MaeI CTAG 4 cut(s) 515, 576, 1127, 1514
MaeII ACGT 3 cut(s) 174, 843, 1690
MaeIII GTNAC 5 cut(s) 287, 401, 621, 1462, 1691
MalI GATC 8 cut(s) 260, 735, 801, 877, 979, 1265, 1359, 1556
MboI GATC 8 cut(s) 258, 733, 799, 875, 977, 1263, 1357, 1554
MboII GAAGA 8 cut(s) 107, 388, 419, 745, 1098, 1424, 1460, 1564
MflI RGATCY 6 cut(s) 258, 799, 977, 1263, 1357, 1554
MhlI GDGCHC 3 cut(s) 688, 1321, 1686
MluCI AATT 9 cut(s) 14, 166, 748, 1010, 1251, 1257, 1328, 1419, 1735
MlyI GAGTC 2 cut(s) 736, 1459
MmeI TCCRAC 3 cut(s) 236, 955, 1184
MreI CGCCGGCG 1 cut(s) 1211
MroI TCCGGA 1 cut(s) 1505
MroNI GCCGGC 1 cut(s) 1211
MroXI GAANNNNTTC 3 cut(s) 18, 1251, 1492
MseI TTAA 3 cut(s) 179, 1286, 1455
MslI CAYNNNNRTG 3 cut(s) 106, 1276, 1595
MspA1I CMGCKG 1 cut(s) 673
MspI CCGG 7 cut(s) 38, 292, 499, 837, 1212, 1506, 1587
MspR9I CCNGG 4 cut(s) 39, 293, 1244, 1305
Mva1269I GAATGC 2 cut(s) 53, 111
MvaI CCWGG 2 cut(s) 1244, 1305
MvnI CGCG 7 cut(s) 490, 595, 1217, 1669, 1697, 1704, 1706
NaeI GCCGGC 1 cut(s) 1213
NciI CCSGG 2 cut(s) 39, 293
NdeII GATC 8 cut(s) 258, 733, 799, 875, 977, 1263, 1357, 1554
NgoMIV GCCGGC 1 cut(s) 1211
NheI GCTAGC 2 cut(s) 514, 1513
NlaIII CATG 8 cut(s) 222, 233, 252, 1022, 1078, 1275, 1328, 1476
NlaIV GGNNCC 6 cut(s) 260, 497, 979, 1248, 1359, 1489
NmuCI GTSAC 2 cut(s) 1462, 1691
NruI TCGCGA 2 cut(s) 490, 595
NspI RCATGY 2 cut(s) 1022, 1275
NspV TTCGAA 2 cut(s) 745, 1255
PagI TCATGA 2 cut(s) 229, 1324
PaqCI CACCTGC 2 cut(s) 88, 106
PciI ACATGT 2 cut(s) 1018, 1271
PcsI WCGNNNNNNNCGW 1 cut(s) 762
PctI GAATGC 2 cut(s) 53, 111
PdiI GCCGGC 1 cut(s) 1213
PdmI GAANNNNTTC 3 cut(s) 18, 1251, 1492
PfeI GAWTC 5 cut(s) 276, 473, 610, 779, 1600
PflFI GACNNNGTC 1 cut(s) 220
PkrI GCNGC 9 cut(s) 94, 534, 915, 934, 954, 1080, 1089, 1623, 1699
PleI GAGTC 2 cut(s) 735, 1459
PpsI GAGTC 2 cut(s) 735, 1459
PscI ACATGT 2 cut(s) 1018, 1271
Psp124BI GAGCTC 1 cut(s) 688
Psp6I CCWGG 2 cut(s) 1242, 1303
PspGI CCWGG 2 cut(s) 1242, 1303
PspN4I GGNNCC 6 cut(s) 260, 497, 979, 1248, 1359, 1489
PspPI GGNCC 4 cut(s) 1206, 1443, 1503, 1633
PstNI CAGNNNCTG 1 cut(s) 98
PsuI RGATCY 6 cut(s) 258, 799, 977, 1263, 1357, 1554
PsyI GACNNNGTC 1 cut(s) 220
RruI TCGCGA 2 cut(s) 490, 595
RsaI GTAC 5 cut(s) 429, 443, 763, 985, 1675
RsaNI GTAC 5 cut(s) 428, 442, 762, 984, 1674
RseI CAYNNNNRTG 3 cut(s) 106, 1276, 1595
Rsr2I CGGWCCG 2 cut(s) 1503, 1633
RsrII CGGWCCG 2 cut(s) 1503, 1633
SacI GAGCTC 1 cut(s) 688
SalI GTCGAC 1 cut(s) 297
SaqAI TTAA 3 cut(s) 179, 1286, 1455
SatI GCNGC 9 cut(s) 93, 533, 914, 933, 953, 1079, 1088, 1622, 1698
Sau3AI GATC 8 cut(s) 258, 733, 799, 875, 977, 1263, 1357, 1554
Sau96I GGNCC 4 cut(s) 1206, 1443, 1503, 1633
ScaI AGTACT 1 cut(s) 1675
SchI GAGTC 2 cut(s) 736, 1459
ScrFI CCNGG 4 cut(s) 39, 293, 1244, 1305
SduI GDGCHC 3 cut(s) 688, 1321, 1686
SfaNI GCATC 4 cut(s) 919, 959, 1305, 1637
SfcI CTRYAG 1 cut(s) 1536
SfuI TTCGAA 2 cut(s) 745, 1255
SgrAI CRCCGGYG 1 cut(s) 1211
SinI GGWCC 4 cut(s) 1206, 1443, 1503, 1633
SmiMI CAYNNNNRTG 3 cut(s) 106, 1276, 1595
Sse9I AATT 9 cut(s) 14, 166, 748, 1010, 1251, 1257, 1328, 1419, 1735
SspI AATATT 1 cut(s) 1459
SspMI CTAG 4 cut(s) 515, 576, 1127, 1514
SstI GAGCTC 1 cut(s) 688
StyD4I CCNGG 4 cut(s) 37, 291, 1242, 1303
StyI CCWWGG 1 cut(s) 127
TaaI ACNGT 3 cut(s) 1225, 1291, 1503
TaiI ACGT 3 cut(s) 177, 846, 1693
TaqI TCGA 5 cut(s) 298, 365, 663, 745, 1255
TaqII GACCGA 1 cut(s) 1621
TasI AATT 9 cut(s) 14, 166, 748, 1010, 1251, 1257, 1328, 1419, 1735
TatI WGTACW 3 cut(s) 427, 441, 1673
TauI GCSGC 5 cut(s) 535, 916, 935, 1090, 1700
TfiI GAWTC 5 cut(s) 276, 473, 610, 779, 1600
Tru1I TTAA 3 cut(s) 179, 1286, 1455
Tru9I TTAA 3 cut(s) 179, 1286, 1455
TscAI CASTG 2 cut(s) 856, 1230
TseFI GTSAC 2 cut(s) 1462, 1691
TseI GCWGC 4 cut(s) 92, 952, 1078, 1621
Tsp45I GTSAC 2 cut(s) 1462, 1691
TspGWI ACGGA 4 cut(s) 131, 696, 1510, 1625
TspRI CASTG 2 cut(s) 856, 1230
Tth111I GACNNNGTC 1 cut(s) 220
VneI GTGCAC 1 cut(s) 1682
VpaK11BI GGWCC 4 cut(s) 1206, 1443, 1503, 1633
XapI RAATTY 3 cut(s) 748, 1010, 1257
XceI RCATGY 2 cut(s) 1022, 1275
XcmI CCANNNNNNNNNTGG 2 cut(s) 1257, 1311
XmiI GTMKAC 1 cut(s) 298
XmnI GAANNNNTTC 3 cut(s) 18, 1251, 1492
XspI CTAG 4 cut(s) 515, 576, 1127, 1514
ZraI GACGTC 1 cut(s) 844
ZrmI AGTACT 1 cut(s) 1675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.