FvH4_2g05080

Respiratory-chain NADH dehydrogenase, 49 Kd subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006041
Physical Location & Seq
Forward (+)
4141218 .. 4141946
729 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05080.t1

Sequence Viewer

Length: 729 bp
ATGCATGCCAGTTTCATACGACCAGGTGGAGTGGCACAAGATCTGCCTCTTGGCTTATGTCGAGATATTGATTCTTTTACACAACAATTTGCTTCTCGTATCGACGAATTAGAAGAGATGTTAACCGGCAACCGTATCTGGAAACAACGATTAGTGGATATTGGTACTGTCACTGCACAGCAAGCAAAGGATTGGGGATTCAGTGGTGTAATGTTAAGAGGTTCAGGGGTATGCTGGGATTTGCGAAGAGCAGCACCTTACGATGTTTATGACCAGTTGGATTTTGACGTACCAGTAGGTACCAGAGGAGATTGCTATGATCGTTACTGTATTCGTATTGAAGAGATGCGACAAAGTGTTCGGATCATTGTGCAATGTCTTAATAAAATGCCTAGTGGCATGATCAAAGCCGATGATCGTAAACTATGTCCTCCATCACGATGTCGAATGAAACTATCCATGGAATCCTTAATTCACCATTTCGAACTTTATACAGAAGGTTTTTCCGTACCAGCTTCTTCTACCTATACCGCAGTTGAAGCACCTAAAGGAGAATTTGGTGTCTTTCTGGTCAGTAATGGAAGCAATCGTCCTTACCGTTGTAAAATAAGAGCACCTGGCTTTGCCCATTTACAAGGACTCGATTTTATGTCCAAACATCACATGCTAGCGGATGTGGTCACCATCATAGGTACTCAAGATATTGTGTCTGGAGAGGTGGATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Complex1_49kDa

Protein Analysis

243

Amino Acids

27.26

Weight (kDa)

6.59

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Complex1_49kDa PF00346 1 - 242 8e-106 Respiratory-chain NADH dehydrogenase, 49 Kd subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 299
AccB1I GGYRCC 1 cut(s) 299
AciI CCGC 2 cut(s) 531, 671
AclWI GGATC 1 cut(s) 371
AcsI RAATTY 1 cut(s) 554
AfaI GTAC 5 cut(s) 166, 291, 301, 510, 694
AgsI TTSAA 2 cut(s) 341, 539
AjnI CCWGG 2 cut(s) 22, 616
AluBI AGCT 1 cut(s) 515
AluI AGCT 1 cut(s) 515
Alw21I GWGCWC 1 cut(s) 616
AlwI GGATC 1 cut(s) 371
ApeKI GCWGC 1 cut(s) 251
ApoI RAATTY 1 cut(s) 554
ArsI GACNNNNNNTTYG 2 cut(s) 342, 374
Asp718I GGTACC 1 cut(s) 299
AsuHPI GGTGA 2 cut(s) 467, 673
AsuII TTCGAA 1 cut(s) 483
AsuNHI GCTAGC 1 cut(s) 667
BanI GGYRCC 1 cut(s) 299
Bbv12I GWGCWC 1 cut(s) 616
BbvI GCAGC 1 cut(s) 263
BccI CCATC 2 cut(s) 442, 692
BciT130I CCWGG 2 cut(s) 24, 618
BclI TGATCA 1 cut(s) 402
BfaI CTAG 2 cut(s) 393, 668
BglII AGATCT 1 cut(s) 40
BisI GCNGC 1 cut(s) 252
BlsI GCNGC 1 cut(s) 253
Bme1390I CCNGG 2 cut(s) 24, 618
BmiI GGNNCC 1 cut(s) 301
BmrFI CCNGG 2 cut(s) 24, 618
BmsI GCATC 1 cut(s) 336
BmtI GCTAGC 1 cut(s) 671
Bpu14I TTCGAA 1 cut(s) 483
BpuEI CTTGAG 1 cut(s) 681
BsaJI CCNNGG 1 cut(s) 459
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 3 cut(s) 9, 274, 293
Bse3DI GCAATG 1 cut(s) 380
BseBI CCWGG 2 cut(s) 24, 618
BseDI CCNNGG 1 cut(s) 459
BseGI GGATG 1 cut(s) 679
BseMI GCAATG 1 cut(s) 380
BseNI ACTGG 3 cut(s) 9, 274, 293
BseRI GAGGAG 1 cut(s) 321
BseXI GCAGC 1 cut(s) 263
BseYI CCCAGC 1 cut(s) 234
BsgI GTGCAG 1 cut(s) 159
BshNI GGYRCC 1 cut(s) 299
BsiHKAI GWGCWC 1 cut(s) 616
BsiSI CCGG 1 cut(s) 126
Bsp119I TTCGAA 1 cut(s) 483
Bsp1286I GDGCHC 1 cut(s) 616
Bsp143I GATC 5 cut(s) 40, 319, 363, 402, 415
Bsp19I CCATGG 1 cut(s) 459
BspACI CCGC 2 cut(s) 531, 671
BspLI GGNNCC 1 cut(s) 301
BspOI GCTAGC 1 cut(s) 671
BspPI GGATC 1 cut(s) 371
BspQI GCTCTTC 1 cut(s) 241
BspT104I TTCGAA 1 cut(s) 483
BspT107I GGYRCC 1 cut(s) 299
BsrDI GCAATG 1 cut(s) 380
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 3 cut(s) 9, 274, 293
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 1 cut(s) 459
BssMI GATC 5 cut(s) 40, 319, 363, 402, 415
BssT1I CCWWGG 1 cut(s) 459
Bst2UI CCWGG 2 cut(s) 24, 618
Bst4CI ACNGT 4 cut(s) 134, 169, 329, 599
Bst6I CTCTTC 3 cut(s) 108, 241, 336
BstBI TTCGAA 1 cut(s) 483
BstC8I GCNNGC 3 cut(s) 6, 183, 669
BstDSI CCRYGG 1 cut(s) 459
BstEII GGTNACC 1 cut(s) 679
BstF5I GGATG 1 cut(s) 679
BstKTI GATC 5 cut(s) 43, 322, 366, 405, 418
BstMBI GATC 5 cut(s) 40, 319, 363, 402, 415
BstMWI GCNNNNNNNGC 2 cut(s) 182, 539
BstNI CCWGG 2 cut(s) 24, 618
BstNSI RCATGY 2 cut(s) 8, 667
BstPI GGTNACC 1 cut(s) 679
BstSCI CCNGG 2 cut(s) 22, 616
BstV1I GCAGC 1 cut(s) 263
BstX2I RGATCY 1 cut(s) 40
BstYI RGATCY 1 cut(s) 40
BtgI CCRYGG 1 cut(s) 459
BtsCI GGATG 1 cut(s) 679
BtsI GCAGTG 1 cut(s) 171
BtsIMutI CAGTG 2 cut(s) 171, 208
Cac8I GCNNGC 3 cut(s) 6, 183, 669
Cfr10I RCCGGY 1 cut(s) 125
CsiI ACCWGGT 1 cut(s) 22
Csp6I GTAC 5 cut(s) 165, 290, 300, 509, 693
CviAII CATG 4 cut(s) 5, 400, 460, 664
CviJI RGCY 4 cut(s) 54, 410, 515, 621
CviKI_1 RGCY 4 cut(s) 54, 410, 515, 621
CviQI GTAC 5 cut(s) 165, 290, 300, 509, 693
DpnI GATC 5 cut(s) 42, 321, 365, 404, 417
DpnII GATC 5 cut(s) 40, 319, 363, 402, 415
Eam1104I CTCTTC 3 cut(s) 108, 241, 336
EarI CTCTTC 3 cut(s) 108, 241, 336
Eco130I CCWWGG 1 cut(s) 459
Eco91I GGTNACC 1 cut(s) 679
EcoO65I GGTNACC 1 cut(s) 679
EcoRII CCWGG 2 cut(s) 22, 616
EcoT14I CCWWGG 1 cut(s) 459
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 459
FaeI CATG 4 cut(s) 8, 403, 463, 667
FatI CATG 4 cut(s) 4, 399, 459, 663
FbaI TGATCA 1 cut(s) 402
Fnu4HI GCNGC 1 cut(s) 252
FokI GGATG 1 cut(s) 686
Fsp4HI GCNGC 1 cut(s) 252
FspBI CTAG 2 cut(s) 393, 668
GluI GCNGC 1 cut(s) 252
GsaI CCCAGC 1 cut(s) 238
HapII CCGG 1 cut(s) 126
Hin1II CATG 4 cut(s) 8, 403, 463, 667
HincII GTYRAC 1 cut(s) 123
HindII GTYRAC 1 cut(s) 123
HinfI GANTC 4 cut(s) 71, 198, 464, 639
HpaI GTTAAC 1 cut(s) 123
HpaII CCGG 1 cut(s) 126
HphI GGTGA 2 cut(s) 467, 673
Hpy166II GTNNAC 2 cut(s) 123, 422
Hpy188I TCNGA 1 cut(s) 363
Hpy188III TCNNGA 5 cut(s) 62, 139, 438, 698, 711
Hpy8I GTNNAC 2 cut(s) 123, 422
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 1 cut(s) 491
HpyCH4III ACNGT 4 cut(s) 134, 169, 329, 599
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 3 cut(s) 4, 176, 373
HpyF10VI GCNNNNNNNGC 2 cut(s) 182, 539
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 4 cut(s) 8, 403, 463, 667
KpnI GGTACC 1 cut(s) 303
Ksp22I TGATCA 1 cut(s) 402
KspAI GTTAAC 1 cut(s) 123
Kzo9I GATC 5 cut(s) 40, 319, 363, 402, 415
LguI GCTCTTC 1 cut(s) 241
Lsp1109I GCAGC 1 cut(s) 263
LweI GCATC 1 cut(s) 336
MabI ACCWGGT 1 cut(s) 22
MaeI CTAG 2 cut(s) 393, 668
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 3 cut(s) 169, 323, 679
MalI GATC 5 cut(s) 42, 321, 365, 404, 417
MboI GATC 5 cut(s) 40, 319, 363, 402, 415
MboII GAAGA 4 cut(s) 125, 258, 353, 510
MflI RGATCY 1 cut(s) 40
MhlI GDGCHC 1 cut(s) 616
MluCI AATT 4 cut(s) 86, 107, 471, 554
MlyI GAGTC 1 cut(s) 633
MmeI TCCRAC 1 cut(s) 258
MnlI CCTC 5 cut(s) 57, 212, 299, 441, 709
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 4 cut(s) 122, 215, 381, 470
MslI CAYNNNNRTG 1 cut(s) 439
MspI CCGG 1 cut(s) 126
MspR9I CCNGG 2 cut(s) 24, 618
MvaI CCWGG 2 cut(s) 24, 618
MwoI GCNNNNNNNGC 2 cut(s) 182, 539
NcoI CCATGG 1 cut(s) 459
NdeII GATC 5 cut(s) 40, 319, 363, 402, 415
NheI GCTAGC 1 cut(s) 667
NlaIII CATG 4 cut(s) 8, 403, 463, 667
NlaIV GGNNCC 1 cut(s) 301
NmuCI GTSAC 2 cut(s) 169, 679
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 8, 667
NspV TTCGAA 1 cut(s) 483
PaeI GCATGC 1 cut(s) 8
PciSI GCTCTTC 1 cut(s) 241
PcsI WCGNNNNNNNCGW 1 cut(s) 595
PfeI GAWTC 3 cut(s) 71, 198, 464
PkrI GCNGC 1 cut(s) 253
PleI GAGTC 1 cut(s) 633
PpsI GAGTC 1 cut(s) 633
Psp6I CCWGG 2 cut(s) 22, 616
PspEI GGTNACC 1 cut(s) 679
PspFI CCCAGC 1 cut(s) 234
PspGI CCWGG 2 cut(s) 22, 616
PspN4I GGNNCC 1 cut(s) 301
PsuI RGATCY 1 cut(s) 40
RsaI GTAC 5 cut(s) 166, 291, 301, 510, 694
RsaNI GTAC 5 cut(s) 165, 290, 300, 509, 693
RseI CAYNNNNRTG 1 cut(s) 439
SapI GCTCTTC 1 cut(s) 241
SaqAI TTAA 4 cut(s) 122, 215, 381, 470
SatI GCNGC 1 cut(s) 252
Sau3AI GATC 5 cut(s) 40, 319, 363, 402, 415
SchI GAGTC 1 cut(s) 633
ScrFI CCNGG 2 cut(s) 24, 618
SduI GDGCHC 1 cut(s) 616
SexAI ACCWGGT 1 cut(s) 22
SfaNI GCATC 1 cut(s) 336
SfuI TTCGAA 1 cut(s) 483
SmiMI CAYNNNNRTG 1 cut(s) 439
SmlI CTYRAG 1 cut(s) 696
SmoI CTYRAG 1 cut(s) 696
SphI GCATGC 1 cut(s) 8
Sse9I AATT 4 cut(s) 86, 107, 471, 554
SsiI CCGC 2 cut(s) 531, 671
SspMI CTAG 2 cut(s) 393, 668
StyD4I CCNGG 2 cut(s) 22, 616
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 4 cut(s) 134, 169, 329, 599
TaiI ACGT 1 cut(s) 291
TaqI TCGA 5 cut(s) 61, 102, 445, 483, 642
TasI AATT 4 cut(s) 86, 107, 471, 554
TfiI GAWTC 3 cut(s) 71, 198, 464
Tru1I TTAA 4 cut(s) 122, 215, 381, 470
Tru9I TTAA 4 cut(s) 122, 215, 381, 470
TscAI CASTG 2 cut(s) 178, 208
TseFI GTSAC 2 cut(s) 169, 679
TseI GCWGC 1 cut(s) 251
Tsp45I GTSAC 2 cut(s) 169, 679
TspDTI ATGAA 2 cut(s) 4, 464
TspGWI ACGGA 1 cut(s) 496
TspRI CASTG 2 cut(s) 178, 208
XapI RAATTY 1 cut(s) 554
XceI RCATGY 2 cut(s) 8, 667
XspI CTAG 2 cut(s) 393, 668
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.