Rw0G023300

Respiratory-chain NADH dehydrogenase, 49 Kd subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig01079
OG0006041
Physical Location & Seq
Forward (+)
183073 .. 186274
3202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G023300.1

Sequence Viewer

Length: 1152 bp
ATGACGACTAGGAACGGGCAAATAAAAAATTTCACTTCGAATTCCGGACCTCAACATCCTGCTGCTCATGGTGTTTCACGATCAGTATTGGAAATGAACGGAGAAGTGGTGGAACGTGCGGAACCACATATTGGATCACTCCAAGGGACTGAGAAATTAATAGAGTACAAAACTTATCTTCAAGCTTTACCTTATTCTGATCAACACGCTCATTCTTCAGCCGTAGAGAGACTTTTGAATTGCGAGGTACCATTACGAGCTCAATATATACGAGTGTTATTCCGTGAAATAACTCGAATTTCAAATCATTCACTTGCTTTAACTACTCATGCTATGGATGTGGGAGCATTAACTCCGTTTCTGTGGGCTTTTGAGGAGCGGGAGAAATTGTTGGAATTCTATGAAAGAGTCTCAGGAGCCAGGATGCATGCCAGTTTCATACGACCAGGTGGAGTGGCACAAGATCTGCCTCTTGGCTTATGTCGAGATATTGATTCTTTTACACAACAATTTGCTTCTCGTATCGACGAATTAGAAGAGATGTTAACCGGCAACCGTATCTGGAAACAACGATTAGTGGATATTGGTACTGTCACTGCACAGCAAGCAAAGGATTGGGGATTCAGTGGTGTAATGTTAAGAGGTTCAGGGGTATGCTGGGATTTGCGAAGAGCAGCACCTTACGATGTTTATGACCAGTTGGATTTTGACGTACCAGTAGGTACCAGAGGAGATTGCTATGATCGTTACTGTATTCGTATTGAAGAGATGCGACAAAGTGTTCGGATCATTGTGCAATGTCTTAATAAAATGCCTAGTGGCATGATCAAAGCTGATGATCGTAAACTATGTCCTCCATCACGAGGTCGAATGAAACTATCCATGGAATCCTTAATTCACCATTTCGAACTTTATACAGAAGGTTTTTCCGTACCAGCTTCTTCTACCTATACCGCAGTTGAAGCACCTAAAGGAGAATTTGGTGTCTTTCTGGTCAGTAATGGAAGCAATCGTCCTTACCGTTGTAAAATAAGAGCACCTGGCTTTGCCCATTTACAAGGACTCGATTTTATGTCCAAACATCACATGCTAGCGGATGTGGTCACCATCATAGGTACTCAAGATATTGTGTCTGGAGAGGTGGATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Complex1_49kDa

Protein Analysis

383

Amino Acids

43.16

Weight (kDa)

6.68

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Complex1_49kDa PF00346 113 - 383 1.8e-121 Respiratory-chain NADH dehydrogenase, 49 Kd subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 247, 722
AccB1I GGYRCC 2 cut(s) 247, 722
AccB7I CCANNNNNTGG 1 cut(s) 131
AccBSI CCGCTC 1 cut(s) 379
AccIII TCCGGA 1 cut(s) 44
AciI CCGC 4 cut(s) 119, 379, 954, 1094
AclWI GGATC 2 cut(s) 142, 794
AcsI RAATTY 5 cut(s) 28, 40, 297, 395, 977
AcuI CTGAAG 1 cut(s) 201
AfaI GTAC 7 cut(s) 167, 249, 589, 714, 724, 933, 1117
AfiI CCNNNNNNNGG 2 cut(s) 131, 863
AgsI TTSAA 5 cut(s) 182, 238, 303, 764, 962
AjnI CCWGG 3 cut(s) 419, 445, 1039
AjuI GAANNNNNNNTTGG 2 cut(s) 114, 146
AluBI AGCT 4 cut(s) 185, 260, 833, 938
AluI AGCT 4 cut(s) 185, 260, 833, 938
Alw21I GWGCWC 2 cut(s) 262, 1039
Alw26I GTCTC 2 cut(s) 223, 415
AlwI GGATC 2 cut(s) 142, 794
Aor13HI TCCGGA 1 cut(s) 44
ApeKI GCWGC 2 cut(s) 62, 674
ApoI RAATTY 5 cut(s) 28, 40, 297, 395, 977
ArsI GACNNNNNNTTYG 2 cut(s) 765, 797
AseI ATTAAT 1 cut(s) 158
Asp718I GGTACC 2 cut(s) 247, 722
AspS9I GGNCC 1 cut(s) 47
AsuHPI GGTGA 2 cut(s) 890, 1096
AsuII TTCGAA 2 cut(s) 38, 906
AsuNHI GCTAGC 1 cut(s) 1090
AvaII GGWCC 1 cut(s) 47
BanI GGYRCC 2 cut(s) 247, 722
BanII GRGCYC 1 cut(s) 262
BauI CACGAG 1 cut(s) 861
Bbv12I GWGCWC 2 cut(s) 262, 1039
BbvI GCAGC 2 cut(s) 49, 686
BccI CCATC 2 cut(s) 865, 1115
BceAI ACGGC 1 cut(s) 206
BciT130I CCWGG 3 cut(s) 421, 447, 1041
BclI TGATCA 2 cut(s) 199, 825
BcoDI GTCTC 2 cut(s) 223, 415
BfaI CTAG 3 cut(s) 9, 816, 1091
BglII AGATCT 1 cut(s) 463
BisI GCNGC 2 cut(s) 63, 675
BlsI GCNGC 2 cut(s) 64, 676
Bme1390I CCNGG 3 cut(s) 421, 447, 1041
Bme18I GGWCC 1 cut(s) 47
BmgT120I GGNCC 1 cut(s) 47
BmiI GGNNCC 4 cut(s) 123, 249, 418, 724
BmrFI CCNGG 3 cut(s) 421, 447, 1041
BmsI GCATC 2 cut(s) 414, 759
BmtI GCTAGC 1 cut(s) 1094
Bpu14I TTCGAA 2 cut(s) 38, 906
BpuEI CTTGAG 1 cut(s) 1104
BsaJI CCNNGG 2 cut(s) 142, 882
BsaWI WCCGGW 1 cut(s) 44
BsaXI ACNNNNNCTCC 4 cut(s) 93, 123, 374, 404
Bsc4I CCNNNNNNNGG 2 cut(s) 131, 863
Bse118I RCCGGY 1 cut(s) 548
Bse1I ACTGG 3 cut(s) 432, 697, 716
Bse3DI GCAATG 1 cut(s) 803
BseAI TCCGGA 1 cut(s) 44
BseBI CCWGG 3 cut(s) 421, 447, 1041
BseDI CCNNGG 2 cut(s) 142, 882
BseGI GGATG 4 cut(s) 55, 343, 429, 1102
BseLI CCNNNNNNNGG 2 cut(s) 131, 863
BseMI GCAATG 1 cut(s) 803
BseMII CTCAG 2 cut(s) 141, 426
BseNI ACTGG 3 cut(s) 432, 697, 716
BseRI GAGGAG 2 cut(s) 389, 744
BseXI GCAGC 2 cut(s) 49, 686
BseYI CCCAGC 1 cut(s) 657
BsgI GTGCAG 1 cut(s) 582
BshNI GGYRCC 2 cut(s) 247, 722
BsiHKAI GWGCWC 2 cut(s) 262, 1039
BsiSI CCGG 2 cut(s) 45, 549
BslFI GGGAC 1 cut(s) 160
BslI CCNNNNNNNGG 2 cut(s) 131, 863
BsmAI GTCTC 2 cut(s) 223, 415
BsmFI GGGAC 1 cut(s) 160
Bsp119I TTCGAA 2 cut(s) 38, 906
Bsp1286I GDGCHC 2 cut(s) 262, 1039
Bsp13I TCCGGA 1 cut(s) 44
Bsp143I GATC 8 cut(s) 80, 134, 199, 463, 742, 786, 825, 838
Bsp19I CCATGG 1 cut(s) 882
BspACI CCGC 4 cut(s) 119, 379, 954, 1094
BspCNI CTCAG 2 cut(s) 142, 425
BspEI TCCGGA 1 cut(s) 44
BspLI GGNNCC 4 cut(s) 123, 249, 418, 724
BspOI GCTAGC 1 cut(s) 1094
BspPI GGATC 2 cut(s) 142, 794
BspQI GCTCTTC 1 cut(s) 664
BspT104I TTCGAA 2 cut(s) 38, 906
BspT107I GGYRCC 2 cut(s) 247, 722
BsrBI CCGCTC 1 cut(s) 379
BsrDI GCAATG 1 cut(s) 803
BsrFI RCCGGY 1 cut(s) 548
BsrI ACTGG 3 cut(s) 432, 697, 716
BssAI RCCGGY 1 cut(s) 548
BssECI CCNNGG 2 cut(s) 142, 882
BssMI GATC 8 cut(s) 80, 134, 199, 463, 742, 786, 825, 838
BssSI CACGAG 1 cut(s) 861
BssT1I CCWWGG 2 cut(s) 142, 882
Bst2BI CACGAG 1 cut(s) 861
Bst2UI CCWGG 3 cut(s) 421, 447, 1041
Bst4CI ACNGT 4 cut(s) 557, 592, 752, 1022
Bst6I CTCTTC 3 cut(s) 531, 664, 759
BstBI TTCGAA 2 cut(s) 38, 906
BstC8I GCNNGC 3 cut(s) 429, 606, 1092
BstDEI CTNAG 2 cut(s) 150, 412
BstDSI CCRYGG 1 cut(s) 882
BstEII GGTNACC 1 cut(s) 1102
BstF5I GGATG 4 cut(s) 55, 343, 429, 1102
BstKTI GATC 8 cut(s) 83, 137, 202, 466, 745, 789, 828, 841
BstMAI GTCTC 2 cut(s) 223, 415
BstMBI GATC 8 cut(s) 80, 134, 199, 463, 742, 786, 825, 838
BstMWI GCNNNNNNNGC 2 cut(s) 605, 962
BstNI CCWGG 3 cut(s) 421, 447, 1041
BstNSI RCATGY 2 cut(s) 431, 1090
BstPI GGTNACC 1 cut(s) 1102
BstSCI CCNGG 3 cut(s) 419, 445, 1039
BstV1I GCAGC 2 cut(s) 49, 686
BstX2I RGATCY 1 cut(s) 463
BstYI RGATCY 1 cut(s) 463
BtgI CCRYGG 1 cut(s) 882
BtsCI GGATG 4 cut(s) 55, 343, 429, 1102
BtsI GCAGTG 1 cut(s) 594
BtsIMutI CAGTG 2 cut(s) 594, 631
Cac8I GCNNGC 3 cut(s) 429, 606, 1092
Cfr10I RCCGGY 1 cut(s) 548
Cfr13I GGNCC 1 cut(s) 47
CsiI ACCWGGT 1 cut(s) 445
Csp6I GTAC 7 cut(s) 166, 248, 588, 713, 723, 932, 1116
CviAII CATG 6 cut(s) 68, 329, 428, 823, 883, 1087
CviJI RGCY 9 cut(s) 185, 221, 260, 368, 419, 477, 833, 938, 1044
CviKI_1 RGCY 9 cut(s) 185, 221, 260, 368, 419, 477, 833, 938, 1044
CviQI GTAC 7 cut(s) 166, 248, 588, 713, 723, 932, 1116
DdeI CTNAG 2 cut(s) 150, 412
DpnI GATC 8 cut(s) 82, 136, 201, 465, 744, 788, 827, 840
DpnII GATC 8 cut(s) 80, 134, 199, 463, 742, 786, 825, 838
Eam1104I CTCTTC 3 cut(s) 531, 664, 759
EarI CTCTTC 3 cut(s) 531, 664, 759
Ecl136II GAGCTC 1 cut(s) 260
Eco130I CCWWGG 2 cut(s) 142, 882
Eco24I GRGCYC 1 cut(s) 262
Eco47I GGWCC 1 cut(s) 47
Eco53kI GAGCTC 1 cut(s) 260
Eco57I CTGAAG 1 cut(s) 201
Eco91I GGTNACC 1 cut(s) 1102
EcoICRI GAGCTC 1 cut(s) 260
EcoO65I GGTNACC 1 cut(s) 1102
EcoRI GAATTC 2 cut(s) 40, 395
EcoRII CCWGG 3 cut(s) 419, 445, 1039
EcoT14I CCWWGG 2 cut(s) 142, 882
EcoT22I ATGCAT 1 cut(s) 429
EcoT38I GRGCYC 1 cut(s) 262
ErhI CCWWGG 2 cut(s) 142, 882
FaeI CATG 6 cut(s) 71, 332, 431, 826, 886, 1090
FaqI GGGAC 1 cut(s) 160
FatI CATG 6 cut(s) 67, 328, 427, 822, 882, 1086
FauI CCCGC 1 cut(s) 372
FbaI TGATCA 2 cut(s) 199, 825
Fnu4HI GCNGC 2 cut(s) 63, 675
FokI GGATG 4 cut(s) 42, 350, 436, 1109
FriOI GRGCYC 1 cut(s) 262
Fsp4HI GCNGC 2 cut(s) 63, 675
FspBI CTAG 3 cut(s) 9, 816, 1091
GluI GCNGC 2 cut(s) 63, 675
GsaI CCCAGC 1 cut(s) 661
HapII CCGG 2 cut(s) 45, 549
Hin1II CATG 6 cut(s) 71, 332, 431, 826, 886, 1090
HincII GTYRAC 1 cut(s) 546
HindII GTYRAC 1 cut(s) 546
HindIII AAGCTT 1 cut(s) 183
HinfI GANTC 5 cut(s) 408, 494, 621, 887, 1062
HpaI GTTAAC 1 cut(s) 546
HpaII CCGG 2 cut(s) 45, 549
HphI GGTGA 2 cut(s) 890, 1096
Hpy166II GTNNAC 2 cut(s) 546, 845
Hpy188I TCNGA 2 cut(s) 199, 786
Hpy188III TCNNGA 8 cut(s) 45, 78, 414, 485, 562, 861, 1121, 1134
Hpy8I GTNNAC 2 cut(s) 546, 845
Hpy99I CGWCG 1 cut(s) 530
HpyAV CCTTC 1 cut(s) 914
HpyCH4III ACNGT 4 cut(s) 557, 592, 752, 1022
HpyCH4IV ACGT 2 cut(s) 115, 711
HpyCH4V TGCA 3 cut(s) 427, 599, 796
HpyF10VI GCNNNNNNNGC 2 cut(s) 605, 962
HpyF3I CTNAG 2 cut(s) 150, 412
HpySE526I ACGT 2 cut(s) 115, 711
Hsp92II CATG 6 cut(s) 71, 332, 431, 826, 886, 1090
Kpn2I TCCGGA 1 cut(s) 44
KpnI GGTACC 2 cut(s) 251, 726
Ksp22I TGATCA 2 cut(s) 199, 825
KspAI GTTAAC 1 cut(s) 546
Kzo9I GATC 8 cut(s) 80, 134, 199, 463, 742, 786, 825, 838
LguI GCTCTTC 1 cut(s) 664
LmnI GCTCC 3 cut(s) 344, 376, 416
Lsp1109I GCAGC 2 cut(s) 49, 686
LweI GCATC 2 cut(s) 414, 759
MabI ACCWGGT 1 cut(s) 445
MaeI CTAG 3 cut(s) 9, 816, 1091
MaeII ACGT 2 cut(s) 115, 711
MaeIII GTNAC 3 cut(s) 592, 746, 1102
MalI GATC 8 cut(s) 82, 136, 201, 465, 744, 788, 827, 840
MbiI CCGCTC 1 cut(s) 379
MboI GATC 8 cut(s) 80, 134, 199, 463, 742, 786, 825, 838
MboII GAAGA 6 cut(s) 170, 207, 548, 681, 776, 933
MflI RGATCY 1 cut(s) 463
MhlI GDGCHC 2 cut(s) 262, 1039
MlyI GAGTC 2 cut(s) 417, 1056
MmeI TCCRAC 2 cut(s) 372, 681
MnlI CCTC 9 cut(s) 60, 238, 367, 480, 635, 722, 857, 864, 1132
Mph1103I ATGCAT 1 cut(s) 429
MroI TCCGGA 1 cut(s) 44
MseI TTAA 7 cut(s) 158, 320, 350, 545, 638, 804, 893
MspI CCGG 2 cut(s) 45, 549
MspR9I CCNGG 3 cut(s) 421, 447, 1041
MvaI CCWGG 3 cut(s) 421, 447, 1041
MwoI GCNNNNNNNGC 2 cut(s) 605, 962
NcoI CCATGG 1 cut(s) 882
NdeII GATC 8 cut(s) 80, 134, 199, 463, 742, 786, 825, 838
NheI GCTAGC 1 cut(s) 1090
NlaIII CATG 6 cut(s) 71, 332, 431, 826, 886, 1090
NlaIV GGNNCC 4 cut(s) 123, 249, 418, 724
NmuCI GTSAC 2 cut(s) 592, 1102
NsiI ATGCAT 1 cut(s) 429
NspI RCATGY 2 cut(s) 431, 1090
NspV TTCGAA 2 cut(s) 38, 906
PaeI GCATGC 1 cut(s) 431
PciSI GCTCTTC 1 cut(s) 664
PcsI WCGNNNNNNNCGW 1 cut(s) 1018
PfeI GAWTC 3 cut(s) 494, 621, 887
PflMI CCANNNNNTGG 1 cut(s) 131
PkrI GCNGC 2 cut(s) 64, 676
PleI GAGTC 2 cut(s) 416, 1056
PpsI GAGTC 2 cut(s) 416, 1056
PshBI ATTAAT 1 cut(s) 158
Psp124BI GAGCTC 1 cut(s) 262
Psp6I CCWGG 3 cut(s) 419, 445, 1039
PspEI GGTNACC 1 cut(s) 1102
PspFI CCCAGC 1 cut(s) 657
PspGI CCWGG 3 cut(s) 419, 445, 1039
PspN4I GGNNCC 4 cut(s) 123, 249, 418, 724
PspPI GGNCC 1 cut(s) 47
PsuI RGATCY 1 cut(s) 463
RsaI GTAC 7 cut(s) 167, 249, 589, 714, 724, 933, 1117
RsaNI GTAC 7 cut(s) 166, 248, 588, 713, 723, 932, 1116
SacI GAGCTC 1 cut(s) 262
SapI GCTCTTC 1 cut(s) 664
SaqAI TTAA 7 cut(s) 158, 320, 350, 545, 638, 804, 893
SatI GCNGC 2 cut(s) 63, 675
Sau3AI GATC 8 cut(s) 80, 134, 199, 463, 742, 786, 825, 838
Sau96I GGNCC 1 cut(s) 47
SchI GAGTC 2 cut(s) 417, 1056
ScrFI CCNGG 3 cut(s) 421, 447, 1041
SduI GDGCHC 2 cut(s) 262, 1039
SexAI ACCWGGT 1 cut(s) 445
SfaNI GCATC 2 cut(s) 414, 759
SfuI TTCGAA 2 cut(s) 38, 906
SinI GGWCC 1 cut(s) 47
SmlI CTYRAG 1 cut(s) 1119
SmoI CTYRAG 1 cut(s) 1119
SphI GCATGC 1 cut(s) 431
SsiI CCGC 4 cut(s) 119, 379, 954, 1094
SspMI CTAG 3 cut(s) 9, 816, 1091
SstI GAGCTC 1 cut(s) 262
StyD4I CCNGG 3 cut(s) 419, 445, 1039
StyI CCWWGG 2 cut(s) 142, 882
TaaI ACNGT 4 cut(s) 557, 592, 752, 1022
TaiI ACGT 2 cut(s) 118, 714
TaqI TCGA 7 cut(s) 38, 295, 484, 525, 868, 906, 1065
TatI WGTACW 1 cut(s) 165
TfiI GAWTC 3 cut(s) 494, 621, 887
Tru1I TTAA 7 cut(s) 158, 320, 350, 545, 638, 804, 893
Tru9I TTAA 7 cut(s) 158, 320, 350, 545, 638, 804, 893
TscAI CASTG 2 cut(s) 601, 631
TseFI GTSAC 2 cut(s) 592, 1102
TseI GCWGC 2 cut(s) 62, 674
Tsp45I GTSAC 2 cut(s) 592, 1102
TspDTI ATGAA 4 cut(s) 110, 417, 427, 887
TspGWI ACGGA 4 cut(s) 114, 272, 345, 919
TspRI CASTG 2 cut(s) 601, 631
Van91I CCANNNNNTGG 1 cut(s) 131
VpaK11BI GGWCC 1 cut(s) 47
VspI ATTAAT 1 cut(s) 158
XapI RAATTY 5 cut(s) 28, 40, 297, 395, 977
XceI RCATGY 2 cut(s) 431, 1090
XspI CTAG 3 cut(s) 9, 816, 1091
Zsp2I ATGCAT 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.