RLG00000000357

Respiratory-chain NADH dehydrogenase, 49 Kd subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006041
Physical Location & Seq
Forward (+)
696415 .. 697233
819 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000357

Sequence Viewer

Length: 819 bp
ATGGATGTGGGAGCATTAACTCCGTTTCTGTGGGCTTTTGAGGAGCGGGAGAAATTGTTGGAATTCTATGAAAGAGTCTCAGGAGCCAGGATGCATGCCAGTTTCATACGACCAGGTGGAGTGGCACAAGATCTGCCTCTTGGCTTATGTCGAGATATTGATTCTTTTACACAACAATTTGCTTCTCGTATCGACGAATTAGAAGAGATGTTAACCGGCAACCGTATCTGGAAACAACGATTAGTGGATATTGGTACTGTCACTGCACAGCAAGCAAAGGATTGGGGATTCAGTGGTGTAATGTTAAGAGGTTCAGGGGTATGCTGGGATTTGCGAAGAGCAGCACCTTACGATGTTTATGACCAGTTGGATTTTGACGTACCAGTAGGTACCAGAGGAGATTGCTATGATCGTTACTGTATTCGTATTGAAGAGATGCGACAAAGTGTTCGGATCATTGTGCAATGTCTTAATAAAATGCCTAGTGGCATGATCAAAGCTGATGATCGTAAACTATGTCCTCCATCACGAGGTCGAATGAAACTATCCATGGAATCCTTAATTCACCATTTCGAACTTTATACAGAAGGTTTTTCCGTACCAGCTTCTTCTACCTATACCGCAGTTGAAGCACCTAAAGGAGAATTTGGTGTCTTTCTGGTCAGTAATGGAAGCAATCGTCCTTACCGTTGTAAAATAAGAGCACCTGGCTTTGCCCATTTACAAGGACTCGATTTTATGTCCAAACATCACATGCTAGCGGATGTGGTCACCATCATAGGTACTCAAGATATTGTGTCTGGAGAGGTGGATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Complex1_49kDa

Protein Analysis

273

Amino Acids

30.76

Weight (kDa)

6.1

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Complex1_49kDa PF00346 2 - 272 5.3e-122 Respiratory-chain NADH dehydrogenase, 49 Kd subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 389
AccB1I GGYRCC 1 cut(s) 389
AccBSI CCGCTC 1 cut(s) 46
AciI CCGC 3 cut(s) 46, 621, 761
AclWI GGATC 1 cut(s) 461
AcsI RAATTY 2 cut(s) 62, 644
AfaI GTAC 5 cut(s) 256, 381, 391, 600, 784
AfiI CCNNNNNNNGG 1 cut(s) 530
AgsI TTSAA 2 cut(s) 431, 629
AjnI CCWGG 3 cut(s) 86, 112, 706
AluBI AGCT 2 cut(s) 500, 605
AluI AGCT 2 cut(s) 500, 605
Alw21I GWGCWC 1 cut(s) 706
Alw26I GTCTC 1 cut(s) 82
AlwI GGATC 1 cut(s) 461
ApeKI GCWGC 1 cut(s) 341
ApoI RAATTY 2 cut(s) 62, 644
ArsI GACNNNNNNTTYG 2 cut(s) 432, 464
Asp718I GGTACC 1 cut(s) 389
AsuHPI GGTGA 2 cut(s) 557, 763
AsuII TTCGAA 1 cut(s) 573
AsuNHI GCTAGC 1 cut(s) 757
BanI GGYRCC 1 cut(s) 389
BauI CACGAG 1 cut(s) 528
Bbv12I GWGCWC 1 cut(s) 706
BbvI GCAGC 1 cut(s) 353
BccI CCATC 2 cut(s) 532, 782
BciT130I CCWGG 3 cut(s) 88, 114, 708
BclI TGATCA 1 cut(s) 492
BcoDI GTCTC 1 cut(s) 82
BfaI CTAG 2 cut(s) 483, 758
BglII AGATCT 1 cut(s) 130
BisI GCNGC 1 cut(s) 342
BlsI GCNGC 1 cut(s) 343
Bme1390I CCNGG 3 cut(s) 88, 114, 708
BmiI GGNNCC 2 cut(s) 85, 391
BmrFI CCNGG 3 cut(s) 88, 114, 708
BmsI GCATC 2 cut(s) 81, 426
BmtI GCTAGC 1 cut(s) 761
Bpu14I TTCGAA 1 cut(s) 573
BpuEI CTTGAG 1 cut(s) 771
BsaJI CCNNGG 1 cut(s) 549
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 1 cut(s) 530
Bse118I RCCGGY 1 cut(s) 215
Bse1I ACTGG 3 cut(s) 99, 364, 383
Bse3DI GCAATG 1 cut(s) 470
BseBI CCWGG 3 cut(s) 88, 114, 708
BseDI CCNNGG 1 cut(s) 549
BseGI GGATG 3 cut(s) 10, 96, 769
BseLI CCNNNNNNNGG 1 cut(s) 530
BseMI GCAATG 1 cut(s) 470
BseMII CTCAG 1 cut(s) 93
BseNI ACTGG 3 cut(s) 99, 364, 383
BseRI GAGGAG 2 cut(s) 56, 411
BseXI GCAGC 1 cut(s) 353
BseYI CCCAGC 1 cut(s) 324
BsgI GTGCAG 1 cut(s) 249
BshNI GGYRCC 1 cut(s) 389
BsiHKAI GWGCWC 1 cut(s) 706
BsiSI CCGG 1 cut(s) 216
BslI CCNNNNNNNGG 1 cut(s) 530
BsmAI GTCTC 1 cut(s) 82
Bsp119I TTCGAA 1 cut(s) 573
Bsp1286I GDGCHC 1 cut(s) 706
Bsp143I GATC 5 cut(s) 130, 409, 453, 492, 505
Bsp19I CCATGG 1 cut(s) 549
BspACI CCGC 3 cut(s) 46, 621, 761
BspCNI CTCAG 1 cut(s) 92
BspLI GGNNCC 2 cut(s) 85, 391
BspOI GCTAGC 1 cut(s) 761
BspPI GGATC 1 cut(s) 461
BspQI GCTCTTC 1 cut(s) 331
BspT104I TTCGAA 1 cut(s) 573
BspT107I GGYRCC 1 cut(s) 389
BsrBI CCGCTC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 470
BsrFI RCCGGY 1 cut(s) 215
BsrI ACTGG 3 cut(s) 99, 364, 383
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 1 cut(s) 549
BssMI GATC 5 cut(s) 130, 409, 453, 492, 505
BssSI CACGAG 1 cut(s) 528
BssT1I CCWWGG 1 cut(s) 549
Bst2BI CACGAG 1 cut(s) 528
Bst2UI CCWGG 3 cut(s) 88, 114, 708
Bst4CI ACNGT 4 cut(s) 224, 259, 419, 689
Bst6I CTCTTC 3 cut(s) 198, 331, 426
BstBI TTCGAA 1 cut(s) 573
BstC8I GCNNGC 3 cut(s) 96, 273, 759
BstDEI CTNAG 1 cut(s) 79
BstDSI CCRYGG 1 cut(s) 549
BstEII GGTNACC 1 cut(s) 769
BstF5I GGATG 3 cut(s) 10, 96, 769
BstKTI GATC 5 cut(s) 133, 412, 456, 495, 508
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 5 cut(s) 130, 409, 453, 492, 505
BstMWI GCNNNNNNNGC 2 cut(s) 272, 629
BstNI CCWGG 3 cut(s) 88, 114, 708
BstNSI RCATGY 2 cut(s) 98, 757
BstPI GGTNACC 1 cut(s) 769
BstSCI CCNGG 3 cut(s) 86, 112, 706
BstV1I GCAGC 1 cut(s) 353
BstX2I RGATCY 1 cut(s) 130
BstYI RGATCY 1 cut(s) 130
BtgI CCRYGG 1 cut(s) 549
BtsCI GGATG 3 cut(s) 10, 96, 769
BtsI GCAGTG 1 cut(s) 261
BtsIMutI CAGTG 2 cut(s) 261, 298
Cac8I GCNNGC 3 cut(s) 96, 273, 759
Cfr10I RCCGGY 1 cut(s) 215
CsiI ACCWGGT 1 cut(s) 112
Csp6I GTAC 5 cut(s) 255, 380, 390, 599, 783
CviAII CATG 4 cut(s) 95, 490, 550, 754
CviJI RGCY 6 cut(s) 35, 86, 144, 500, 605, 711
CviKI_1 RGCY 6 cut(s) 35, 86, 144, 500, 605, 711
CviQI GTAC 5 cut(s) 255, 380, 390, 599, 783
DdeI CTNAG 1 cut(s) 79
DpnI GATC 5 cut(s) 132, 411, 455, 494, 507
DpnII GATC 5 cut(s) 130, 409, 453, 492, 505
Eam1104I CTCTTC 3 cut(s) 198, 331, 426
EarI CTCTTC 3 cut(s) 198, 331, 426
Eco130I CCWWGG 1 cut(s) 549
Eco91I GGTNACC 1 cut(s) 769
EcoO65I GGTNACC 1 cut(s) 769
EcoRI GAATTC 1 cut(s) 62
EcoRII CCWGG 3 cut(s) 86, 112, 706
EcoT14I CCWWGG 1 cut(s) 549
EcoT22I ATGCAT 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 549
FaeI CATG 4 cut(s) 98, 493, 553, 757
FatI CATG 4 cut(s) 94, 489, 549, 753
FauI CCCGC 1 cut(s) 39
FbaI TGATCA 1 cut(s) 492
Fnu4HI GCNGC 1 cut(s) 342
FokI GGATG 3 cut(s) 17, 103, 776
Fsp4HI GCNGC 1 cut(s) 342
FspBI CTAG 2 cut(s) 483, 758
GluI GCNGC 1 cut(s) 342
GsaI CCCAGC 1 cut(s) 328
HapII CCGG 1 cut(s) 216
Hin1II CATG 4 cut(s) 98, 493, 553, 757
HincII GTYRAC 1 cut(s) 213
HindII GTYRAC 1 cut(s) 213
HinfI GANTC 5 cut(s) 75, 161, 288, 554, 729
HpaI GTTAAC 1 cut(s) 213
HpaII CCGG 1 cut(s) 216
HphI GGTGA 2 cut(s) 557, 763
Hpy166II GTNNAC 2 cut(s) 213, 512
Hpy188I TCNGA 1 cut(s) 453
Hpy188III TCNNGA 6 cut(s) 81, 152, 229, 528, 788, 801
Hpy8I GTNNAC 2 cut(s) 213, 512
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 1 cut(s) 581
HpyCH4III ACNGT 4 cut(s) 224, 259, 419, 689
HpyCH4IV ACGT 1 cut(s) 378
HpyCH4V TGCA 3 cut(s) 94, 266, 463
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 629
HpyF3I CTNAG 1 cut(s) 79
HpySE526I ACGT 1 cut(s) 378
Hsp92II CATG 4 cut(s) 98, 493, 553, 757
KpnI GGTACC 1 cut(s) 393
Ksp22I TGATCA 1 cut(s) 492
KspAI GTTAAC 1 cut(s) 213
Kzo9I GATC 5 cut(s) 130, 409, 453, 492, 505
LguI GCTCTTC 1 cut(s) 331
LmnI GCTCC 3 cut(s) 11, 43, 83
Lsp1109I GCAGC 1 cut(s) 353
LweI GCATC 2 cut(s) 81, 426
MabI ACCWGGT 1 cut(s) 112
MaeI CTAG 2 cut(s) 483, 758
MaeII ACGT 1 cut(s) 378
MaeIII GTNAC 3 cut(s) 259, 413, 769
MalI GATC 5 cut(s) 132, 411, 455, 494, 507
MbiI CCGCTC 1 cut(s) 46
MboI GATC 5 cut(s) 130, 409, 453, 492, 505
MboII GAAGA 4 cut(s) 215, 348, 443, 600
MflI RGATCY 1 cut(s) 130
MhlI GDGCHC 1 cut(s) 706
MluCI AATT 6 cut(s) 53, 62, 176, 197, 561, 644
MlyI GAGTC 2 cut(s) 84, 723
MmeI TCCRAC 2 cut(s) 39, 348
MnlI CCTC 7 cut(s) 34, 147, 302, 389, 524, 531, 799
Mph1103I ATGCAT 1 cut(s) 96
MseI TTAA 5 cut(s) 17, 212, 305, 471, 560
MspI CCGG 1 cut(s) 216
MspR9I CCNGG 3 cut(s) 88, 114, 708
MvaI CCWGG 3 cut(s) 88, 114, 708
MwoI GCNNNNNNNGC 2 cut(s) 272, 629
NcoI CCATGG 1 cut(s) 549
NdeII GATC 5 cut(s) 130, 409, 453, 492, 505
NheI GCTAGC 1 cut(s) 757
NlaIII CATG 4 cut(s) 98, 493, 553, 757
NlaIV GGNNCC 2 cut(s) 85, 391
NmuCI GTSAC 2 cut(s) 259, 769
NsiI ATGCAT 1 cut(s) 96
NspI RCATGY 2 cut(s) 98, 757
NspV TTCGAA 1 cut(s) 573
PaeI GCATGC 1 cut(s) 98
PciSI GCTCTTC 1 cut(s) 331
PcsI WCGNNNNNNNCGW 1 cut(s) 685
PfeI GAWTC 3 cut(s) 161, 288, 554
PkrI GCNGC 1 cut(s) 343
PleI GAGTC 2 cut(s) 83, 723
PpsI GAGTC 2 cut(s) 83, 723
Psp6I CCWGG 3 cut(s) 86, 112, 706
PspEI GGTNACC 1 cut(s) 769
PspFI CCCAGC 1 cut(s) 324
PspGI CCWGG 3 cut(s) 86, 112, 706
PspN4I GGNNCC 2 cut(s) 85, 391
PsuI RGATCY 1 cut(s) 130
RsaI GTAC 5 cut(s) 256, 381, 391, 600, 784
RsaNI GTAC 5 cut(s) 255, 380, 390, 599, 783
SapI GCTCTTC 1 cut(s) 331
SaqAI TTAA 5 cut(s) 17, 212, 305, 471, 560
SatI GCNGC 1 cut(s) 342
Sau3AI GATC 5 cut(s) 130, 409, 453, 492, 505
SchI GAGTC 2 cut(s) 84, 723
ScrFI CCNGG 3 cut(s) 88, 114, 708
SduI GDGCHC 1 cut(s) 706
SexAI ACCWGGT 1 cut(s) 112
SfaNI GCATC 2 cut(s) 81, 426
SfuI TTCGAA 1 cut(s) 573
SmlI CTYRAG 1 cut(s) 786
SmoI CTYRAG 1 cut(s) 786
SphI GCATGC 1 cut(s) 98
Sse9I AATT 6 cut(s) 53, 62, 176, 197, 561, 644
SsiI CCGC 3 cut(s) 46, 621, 761
SspMI CTAG 2 cut(s) 483, 758
StyD4I CCNGG 3 cut(s) 86, 112, 706
StyI CCWWGG 1 cut(s) 549
TaaI ACNGT 4 cut(s) 224, 259, 419, 689
TaiI ACGT 1 cut(s) 381
TaqI TCGA 5 cut(s) 151, 192, 535, 573, 732
TasI AATT 6 cut(s) 53, 62, 176, 197, 561, 644
TfiI GAWTC 3 cut(s) 161, 288, 554
Tru1I TTAA 5 cut(s) 17, 212, 305, 471, 560
Tru9I TTAA 5 cut(s) 17, 212, 305, 471, 560
TscAI CASTG 2 cut(s) 268, 298
TseFI GTSAC 2 cut(s) 259, 769
TseI GCWGC 1 cut(s) 341
Tsp45I GTSAC 2 cut(s) 259, 769
TspDTI ATGAA 3 cut(s) 84, 94, 554
TspGWI ACGGA 2 cut(s) 12, 586
TspRI CASTG 2 cut(s) 268, 298
XapI RAATTY 2 cut(s) 62, 644
XceI RCATGY 2 cut(s) 98, 757
XspI CTAG 2 cut(s) 483, 758
Zsp2I ATGCAT 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.