RCHIOBHM_MTG0498261

Respiratory-chain NADH dehydrogenase, 49 Kd subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Mt
OG0006041
Physical Location & Seq
Reverse (-)
47486 .. 48600
1115 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15601

Sequence Viewer

Length: 963 bp
ATGATGGCCCAAGAACACGCTCATTCTTCAGCCGTAGAGAGACTTTTGAATTGCGAGGTACCATTACGAGCTCAATATATACGAGTGTTATTCCGTGAAATAACTCGAATTTCAAATCATTCACTTGCTTTAACTACTCATGCTATGGATGTGGGAGCATTAACTCCGTTTCTGTGGGCTTTTGAGGAGCGGGAGAAATTGTTGGAATTCTATGAAAGAGTCTCAGGAGCCAGGATGCATGCCAGTTTCATACGACCAGGTGGAGTGGCACAAGATCTGCCTCTTGGCTTATGTCGAGATATTGATTCTTTTACACAACAATTTGCTTCTCGTATCGACGAATTAGAAGAGATGTTAACCGGCAACCGTATCTGGAAACAACGATTAGTGGATATTGGTACTGTCACTGCACAGCAAGCAAAGGATTGGGGATTCAGTGGTGTAATGTTAAGAGGTTCAGGGGTATGCTGGGATTTGCGAAGAGCAGCACCTTACGATGTTTATGACCAGTTGGATTTTGACGTACCAGTAGGTACCAGAGGAGATTGCTATGATCGTTACTGTATTCGTATTGAAGAGATGCGACAAAGTGTTCGGATCATTGTGCAATGTCTTAATAAAATGCCTAGTGGCATGATCAAAGCTGATGATCGTAAACTATGTCCTCCATCACGAGGTCGAATGAAACTATCCATGGAATCCTTAATTCACCATTTCGAACTTTATACAGAAGGTTTTTCCGTACCAGCTTCTTCTACCTATACCGCAGTTGAAGCACCTAAAGGAGAATTTGGTGTCTTTCTGGTCAGTAATGGAAGCAATCGTCCTTACCGTTGTAAAATAAGAGCACCTGGCTTTGCCCATTTACAAGGACTCGATTTTATGTCCAAACATCACATGCTAGCGGATGTGGTCACCATCATAGGTACTCAAGATATTGTGTCTGGAGAGGTGGATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Complex1_49kDa

Protein Analysis

320

Amino Acids

36.26

Weight (kDa)

6.56

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Complex1_49kDa PF00346 50 - 320 9.8e-122 Respiratory-chain NADH dehydrogenase, 49 Kd subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 58, 533
AccB1I GGYRCC 2 cut(s) 58, 533
AccBSI CCGCTC 1 cut(s) 190
AciI CCGC 3 cut(s) 190, 765, 905
AclWI GGATC 1 cut(s) 605
AcsI RAATTY 3 cut(s) 108, 206, 788
AcuI CTGAAG 1 cut(s) 12
AfaI GTAC 6 cut(s) 60, 400, 525, 535, 744, 928
AfiI CCNNNNNNNGG 1 cut(s) 674
AgsI TTSAA 4 cut(s) 49, 114, 575, 773
AjnI CCWGG 3 cut(s) 230, 256, 850
AluBI AGCT 3 cut(s) 71, 644, 749
AluI AGCT 3 cut(s) 71, 644, 749
Alw21I GWGCWC 2 cut(s) 73, 850
Alw26I GTCTC 2 cut(s) 34, 226
AlwI GGATC 1 cut(s) 605
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 1 cut(s) 485
ApoI RAATTY 3 cut(s) 108, 206, 788
ArsI GACNNNNNNTTYG 2 cut(s) 576, 608
Asp718I GGTACC 2 cut(s) 58, 533
AspS9I GGNCC 1 cut(s) 7
AsuHPI GGTGA 2 cut(s) 701, 907
AsuII TTCGAA 1 cut(s) 717
AsuNHI GCTAGC 1 cut(s) 901
BanI GGYRCC 2 cut(s) 58, 533
BanII GRGCYC 1 cut(s) 73
BauI CACGAG 1 cut(s) 672
Bbv12I GWGCWC 2 cut(s) 73, 850
BbvI GCAGC 1 cut(s) 497
BccI CCATC 2 cut(s) 676, 926
BceAI ACGGC 1 cut(s) 17
BciT130I CCWGG 3 cut(s) 232, 258, 852
BclI TGATCA 1 cut(s) 636
BcoDI GTCTC 2 cut(s) 34, 226
BfaI CTAG 2 cut(s) 627, 902
BglII AGATCT 1 cut(s) 274
BisI GCNGC 1 cut(s) 486
BlsI GCNGC 1 cut(s) 487
Bme1390I CCNGG 3 cut(s) 232, 258, 852
BmgT120I GGNCC 1 cut(s) 7
BmiI GGNNCC 3 cut(s) 60, 229, 535
BmrFI CCNGG 3 cut(s) 232, 258, 852
BmsI GCATC 2 cut(s) 225, 570
BmtI GCTAGC 1 cut(s) 905
Bpu14I TTCGAA 1 cut(s) 717
BpuEI CTTGAG 1 cut(s) 915
BsaJI CCNNGG 1 cut(s) 693
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 1 cut(s) 674
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 3 cut(s) 243, 508, 527
Bse3DI GCAATG 1 cut(s) 614
BseBI CCWGG 3 cut(s) 232, 258, 852
BseDI CCNNGG 1 cut(s) 693
BseGI GGATG 3 cut(s) 154, 240, 913
BseLI CCNNNNNNNGG 1 cut(s) 674
BseMI GCAATG 1 cut(s) 614
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 3 cut(s) 243, 508, 527
BseRI GAGGAG 2 cut(s) 200, 555
BseXI GCAGC 1 cut(s) 497
BseYI CCCAGC 1 cut(s) 468
BsgI GTGCAG 1 cut(s) 393
BshFI GGCC 1 cut(s) 8
BshNI GGYRCC 2 cut(s) 58, 533
BsiHKAI GWGCWC 2 cut(s) 73, 850
BsiSI CCGG 1 cut(s) 360
BslI CCNNNNNNNGG 1 cut(s) 674
BsmAI GTCTC 2 cut(s) 34, 226
BsnI GGCC 1 cut(s) 8
Bsp119I TTCGAA 1 cut(s) 717
Bsp1286I GDGCHC 2 cut(s) 73, 850
Bsp143I GATC 5 cut(s) 274, 553, 597, 636, 649
Bsp19I CCATGG 1 cut(s) 693
BspACI CCGC 3 cut(s) 190, 765, 905
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 1 cut(s) 236
BspLI GGNNCC 3 cut(s) 60, 229, 535
BspOI GCTAGC 1 cut(s) 905
BspPI GGATC 1 cut(s) 605
BspQI GCTCTTC 1 cut(s) 475
BspT104I TTCGAA 1 cut(s) 717
BspT107I GGYRCC 2 cut(s) 58, 533
BsrBI CCGCTC 1 cut(s) 190
BsrDI GCAATG 1 cut(s) 614
BsrFI RCCGGY 1 cut(s) 359
BsrI ACTGG 3 cut(s) 243, 508, 527
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 1 cut(s) 693
BssMI GATC 5 cut(s) 274, 553, 597, 636, 649
BssSI CACGAG 1 cut(s) 672
BssT1I CCWWGG 1 cut(s) 693
Bst2BI CACGAG 1 cut(s) 672
Bst2UI CCWGG 3 cut(s) 232, 258, 852
Bst4CI ACNGT 4 cut(s) 368, 403, 563, 833
Bst6I CTCTTC 3 cut(s) 342, 475, 570
BstBI TTCGAA 1 cut(s) 717
BstC8I GCNNGC 3 cut(s) 240, 417, 903
BstDEI CTNAG 1 cut(s) 223
BstDSI CCRYGG 1 cut(s) 693
BstEII GGTNACC 1 cut(s) 913
BstF5I GGATG 3 cut(s) 154, 240, 913
BstKTI GATC 5 cut(s) 277, 556, 600, 639, 652
BstMAI GTCTC 2 cut(s) 34, 226
BstMBI GATC 5 cut(s) 274, 553, 597, 636, 649
BstMWI GCNNNNNNNGC 2 cut(s) 416, 773
BstNI CCWGG 3 cut(s) 232, 258, 852
BstNSI RCATGY 2 cut(s) 242, 901
BstPI GGTNACC 1 cut(s) 913
BstSCI CCNGG 3 cut(s) 230, 256, 850
BstV1I GCAGC 1 cut(s) 497
BstX2I RGATCY 1 cut(s) 274
BstYI RGATCY 1 cut(s) 274
BsuRI GGCC 1 cut(s) 8
BtgI CCRYGG 1 cut(s) 693
BtsCI GGATG 3 cut(s) 154, 240, 913
BtsI GCAGTG 1 cut(s) 405
BtsIMutI CAGTG 2 cut(s) 405, 442
Cac8I GCNNGC 3 cut(s) 240, 417, 903
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 1 cut(s) 7
CsiI ACCWGGT 1 cut(s) 256
Csp6I GTAC 6 cut(s) 59, 399, 524, 534, 743, 927
CviAII CATG 5 cut(s) 140, 239, 634, 694, 898
CviJI RGCY 9 cut(s) 8, 32, 71, 179, 230, 288, 644, 749, 855
CviKI_1 RGCY 9 cut(s) 8, 32, 71, 179, 230, 288, 644, 749, 855
CviQI GTAC 6 cut(s) 59, 399, 524, 534, 743, 927
DdeI CTNAG 1 cut(s) 223
DpnI GATC 5 cut(s) 276, 555, 599, 638, 651
DpnII GATC 5 cut(s) 274, 553, 597, 636, 649
Eam1104I CTCTTC 3 cut(s) 342, 475, 570
EarI CTCTTC 3 cut(s) 342, 475, 570
Ecl136II GAGCTC 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 693
Eco24I GRGCYC 1 cut(s) 73
Eco53kI GAGCTC 1 cut(s) 71
Eco57I CTGAAG 1 cut(s) 12
Eco91I GGTNACC 1 cut(s) 913
EcoICRI GAGCTC 1 cut(s) 71
EcoO65I GGTNACC 1 cut(s) 913
EcoRI GAATTC 1 cut(s) 206
EcoRII CCWGG 3 cut(s) 230, 256, 850
EcoT14I CCWWGG 1 cut(s) 693
EcoT22I ATGCAT 1 cut(s) 240
EcoT38I GRGCYC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 693
FaeI CATG 5 cut(s) 143, 242, 637, 697, 901
FatI CATG 5 cut(s) 139, 238, 633, 693, 897
FauI CCCGC 1 cut(s) 183
FbaI TGATCA 1 cut(s) 636
Fnu4HI GCNGC 1 cut(s) 486
FokI GGATG 3 cut(s) 161, 247, 920
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 1 cut(s) 486
FspBI CTAG 2 cut(s) 627, 902
GluI GCNGC 1 cut(s) 486
GsaI CCCAGC 1 cut(s) 472
HaeIII GGCC 1 cut(s) 8
HapII CCGG 1 cut(s) 360
Hin1II CATG 5 cut(s) 143, 242, 637, 697, 901
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HinfI GANTC 5 cut(s) 219, 305, 432, 698, 873
HpaI GTTAAC 1 cut(s) 357
HpaII CCGG 1 cut(s) 360
HphI GGTGA 2 cut(s) 701, 907
Hpy166II GTNNAC 2 cut(s) 357, 656
Hpy188I TCNGA 1 cut(s) 597
Hpy188III TCNNGA 6 cut(s) 225, 296, 373, 672, 932, 945
Hpy8I GTNNAC 2 cut(s) 357, 656
Hpy99I CGWCG 1 cut(s) 341
HpyAV CCTTC 1 cut(s) 725
HpyCH4III ACNGT 4 cut(s) 368, 403, 563, 833
HpyCH4IV ACGT 1 cut(s) 522
HpyCH4V TGCA 3 cut(s) 238, 410, 607
HpyF10VI GCNNNNNNNGC 2 cut(s) 416, 773
HpyF3I CTNAG 1 cut(s) 223
HpySE526I ACGT 1 cut(s) 522
Hsp92II CATG 5 cut(s) 143, 242, 637, 697, 901
KpnI GGTACC 2 cut(s) 62, 537
Ksp22I TGATCA 1 cut(s) 636
KspAI GTTAAC 1 cut(s) 357
Kzo9I GATC 5 cut(s) 274, 553, 597, 636, 649
LguI GCTCTTC 1 cut(s) 475
LmnI GCTCC 3 cut(s) 155, 187, 227
Lsp1109I GCAGC 1 cut(s) 497
LweI GCATC 2 cut(s) 225, 570
MabI ACCWGGT 1 cut(s) 256
MaeI CTAG 2 cut(s) 627, 902
MaeII ACGT 1 cut(s) 522
MaeIII GTNAC 3 cut(s) 403, 557, 913
MalI GATC 5 cut(s) 276, 555, 599, 638, 651
MbiI CCGCTC 1 cut(s) 190
MboI GATC 5 cut(s) 274, 553, 597, 636, 649
MboII GAAGA 5 cut(s) 18, 359, 492, 587, 744
MflI RGATCY 1 cut(s) 274
MhlI GDGCHC 2 cut(s) 73, 850
MluCI AATT 8 cut(s) 49, 108, 197, 206, 320, 341, 705, 788
MlyI GAGTC 2 cut(s) 228, 867
MmeI TCCRAC 2 cut(s) 183, 492
MnlI CCTC 8 cut(s) 49, 178, 291, 446, 533, 668, 675, 943
Mph1103I ATGCAT 1 cut(s) 240
MseI TTAA 6 cut(s) 131, 161, 356, 449, 615, 704
MspI CCGG 1 cut(s) 360
MspR9I CCNGG 3 cut(s) 232, 258, 852
MvaI CCWGG 3 cut(s) 232, 258, 852
MwoI GCNNNNNNNGC 2 cut(s) 416, 773
NcoI CCATGG 1 cut(s) 693
NdeII GATC 5 cut(s) 274, 553, 597, 636, 649
NheI GCTAGC 1 cut(s) 901
NlaIII CATG 5 cut(s) 143, 242, 637, 697, 901
NlaIV GGNNCC 3 cut(s) 60, 229, 535
NmuCI GTSAC 2 cut(s) 403, 913
NsiI ATGCAT 1 cut(s) 240
NspI RCATGY 2 cut(s) 242, 901
NspV TTCGAA 1 cut(s) 717
PaeI GCATGC 1 cut(s) 242
PciSI GCTCTTC 1 cut(s) 475
PcsI WCGNNNNNNNCGW 1 cut(s) 829
PfeI GAWTC 3 cut(s) 305, 432, 698
PkrI GCNGC 1 cut(s) 487
PleI GAGTC 2 cut(s) 227, 867
PpsI GAGTC 2 cut(s) 227, 867
Psp124BI GAGCTC 1 cut(s) 73
Psp6I CCWGG 3 cut(s) 230, 256, 850
PspEI GGTNACC 1 cut(s) 913
PspFI CCCAGC 1 cut(s) 468
PspGI CCWGG 3 cut(s) 230, 256, 850
PspN4I GGNNCC 3 cut(s) 60, 229, 535
PspPI GGNCC 1 cut(s) 7
PsuI RGATCY 1 cut(s) 274
RsaI GTAC 6 cut(s) 60, 400, 525, 535, 744, 928
RsaNI GTAC 6 cut(s) 59, 399, 524, 534, 743, 927
SacI GAGCTC 1 cut(s) 73
SapI GCTCTTC 1 cut(s) 475
SaqAI TTAA 6 cut(s) 131, 161, 356, 449, 615, 704
SatI GCNGC 1 cut(s) 486
Sau3AI GATC 5 cut(s) 274, 553, 597, 636, 649
Sau96I GGNCC 1 cut(s) 7
SchI GAGTC 2 cut(s) 228, 867
ScrFI CCNGG 3 cut(s) 232, 258, 852
SduI GDGCHC 2 cut(s) 73, 850
SexAI ACCWGGT 1 cut(s) 256
SfaNI GCATC 2 cut(s) 225, 570
SfuI TTCGAA 1 cut(s) 717
SmlI CTYRAG 1 cut(s) 930
SmoI CTYRAG 1 cut(s) 930
SphI GCATGC 1 cut(s) 242
Sse9I AATT 8 cut(s) 49, 108, 197, 206, 320, 341, 705, 788
SsiI CCGC 3 cut(s) 190, 765, 905
SspMI CTAG 2 cut(s) 627, 902
SstI GAGCTC 1 cut(s) 73
StyD4I CCNGG 3 cut(s) 230, 256, 850
StyI CCWWGG 1 cut(s) 693
TaaI ACNGT 4 cut(s) 368, 403, 563, 833
TaiI ACGT 1 cut(s) 525
TaqI TCGA 6 cut(s) 106, 295, 336, 679, 717, 876
TasI AATT 8 cut(s) 49, 108, 197, 206, 320, 341, 705, 788
TfiI GAWTC 3 cut(s) 305, 432, 698
Tru1I TTAA 6 cut(s) 131, 161, 356, 449, 615, 704
Tru9I TTAA 6 cut(s) 131, 161, 356, 449, 615, 704
TscAI CASTG 2 cut(s) 412, 442
TseFI GTSAC 2 cut(s) 403, 913
TseI GCWGC 1 cut(s) 485
Tsp45I GTSAC 2 cut(s) 403, 913
TspDTI ATGAA 3 cut(s) 228, 238, 698
TspGWI ACGGA 3 cut(s) 83, 156, 730
TspRI CASTG 2 cut(s) 412, 442
XapI RAATTY 3 cut(s) 108, 206, 788
XceI RCATGY 2 cut(s) 242, 901
XspI CTAG 2 cut(s) 627, 902
Zsp2I ATGCAT 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.