FvH4_1g02590

Rap1-interacting factor 1 N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0006378
Physical Location & Seq
Reverse (-)
1450671 .. 1457231
6561 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g02590.t3

Sequence Viewer

Length: 3426 bp
ATGTCGAATTTCTCTGACGAGCTCGAAGAAATCAAATCCCTAATCTCTTCAGGCACCAAATCAAACAAGTCACTCGGCTACTCGACTCTCTCACACCTCCAAGAACAGTCCTCCGACGACCACGCTTTGATTCAAACACTCTCGCTGAGCTCCCGGAGCCTTCTCCGTCGCATCGTCGCCGATGTTCACAGCGACGATGAAGAAATCGCGACGCAGGCTTTGAAATGTTTGGGGTTTATGATATACCATCCCGCAATTGTTGCCGCAATTATAGCGGATGATGTGAAGTTAGTTTTGGATTCATTGGTTAGAGTCATTACGACTACGAAAATGAAGTCGGTTTGTAATTTAGGAGTGTGGTGCATATCTCTGCAACAGCTGAGTGAATCACTTTTGGCTCCTCATTTCCATTCTCTCTTGCGCGCGGTAGTTCATGCCATTGACAATCCCATTGGTTCTCTGTCGACAACTTTTGAGGCTATTCAGGCTGTTATTAAGCTAGCAACTCTATTAAGTGAGAACATGAGAGAATTGTCTCATATATGGGCGCCTCCAATATATAGAAGACTTCTTAGCTTTGACAAGAGAGACAGGGATATGTCTGAAAGGTGTTTATTGAAGATTAGATCTACAATCATACCTCCTCCGATAAGTCTCTCCAAGGTGCTTGTCAAAGATTTAAAGAAAAAGTTGCTCGCTCGGATGCACGATATGCTAACCAGTGGTATGAAGGTTCAAACTATTCAAGCATGGGGATGGTATATCCGTTTACTAGGGTCTCATGCCTTGAAAAACAGACATTTAATTAATGATATGCTGAAAATTCCTGAGAAAACATTTTCAGATCACGACTCACAAGTTCAGATTGCTTCCCAGGTTGCCTGGGAAGGTCTTATCGATGCTCTTATTCACCCACCAATGTTATTGCCGTATACGACAAATGCTGCAAAAGAGGATGGTATGCAAAGAAAGGGAACACAGGAAGTAAACAGTGGTGAAATCCTTAAAACTGGAACTTTTAAAAGCGTAAGGCTCATCATGACACCACTTATAGGCATCATGTTGAGTAACTCTGATGTATCTGTCCACTTGGCCTGCTTGAACACGTGGTGCTATCTGTTACATAAACTTGATACCTCTCTGAATGATTCTTCAATGATCAACTTTGTAGTAGACCCTATCTTTGAAGCAGCTTTTCAACTTGATCCTGATGGTAAGAGCTTCTGGGCTAGGGATGTGTGCATTAATTTGCTTGATGATTTCATATCAGCAAAATGTAAACCTCTAGACTATGACCCACGTAATCAGGTAAGCCAGCAGTTATCAGCCCAAACCTCTTTGAATGCACCCATCATATCTGACAGTTGCTCATGGAAGCAGTATCCTATTAAATGGTTGCCATGGGATCTGAGTATGTTGGACTTTCATTTAAAGATGATCTACAGCCTCATCCTTCAATCACCAAAGAAAACTGTCTGCTATGATAACAGGGTTCCAGCTGATGAGGCTCCCTTGAGGTTATTTAGAGCTGTTTTGAAAGGAGTTGAACTGGAATTCAAGAGGTCATCCATCAATTATGATGATATTATGTTGTGTTTGAATGCAATACTGAAGTTCATAAGAAATATATGTGAAGAAGTAAGTCAGGATGGTTGTGACAGACATGGCTTGTATCATATATCACTTCAGTTTATAGAGGCTGTCACCGAGGAGATAGAACCTACTATAATAGGATCCCCTCTTTACAAGGTGGCATTGAACATCAAGTATTTTGAAAACCTGCAAATGGTTGCTGACATTGGATATGAAAATTCGGGTGTCTGCTCTATTGGTTATATGGATATGGTTTCACCGATGGTTTATTTGAGTGTACTTTACTTCTCGGTGGTGGTTCAGTCAAGCTTGAGTACATCCAAAGCCGACTACATTCGACAGGGAATGCAGAAATATATCAGGTATATGCTATCATCTTTTGGTCCCCTGGAAACTCTCGTGATCGCTACTGAATTATTGTACAAGCACATTGGGCCAGGCTGCTTAACGATGTGGATAGGAATAGCAGAAGGTCTAAAAAGCTACATAAATGATGTGAAGGATCTTTCATTGTTCAAAATAGACTCTGAGAGCAAGAGTTATTATGCCATGAGCCATCTAATATCTTATCCATTGCTTGTATGCTCCTGTGCCCAGAAAGACATTATGTCATCAATGAGTGGAAGTCCCTTGGAAACTCCTTCCCGGCAGTTGAAGGCTGAGCTTGAACAAGTTATCACATTATGGAAGTCCCTCTATGGTTCTATCATTACAGCACTGTCTTGGTATACCACTGTTGGCAGTTTCTCTGAGGACCTAGTTCCTGTATTCAAGAGGTGCCTTGACAAGTATACTAGTATTCTTACATGTGCTAACGAGCTTCACTTAAACTTCAAGGATTTGGATCGTCATCTCATTTCCTTATTTGGTGATGCTTTATTATGTATTCTGAAAAATTCCAGTTCCTCAGGATTAAGTTCAGATGGTAATACCAAGAGTGGCTATGATCACAAGATCTCTGATGGCATTAAAGGCTGCTTGACTTTGGCAATTAGATGTATGAAGTTGTTGCACACAAAGATAGGAACAGATGCACCAATTGGTCTTCCTAGTTGCAGGTTATATTCTGAATTGGCGCTATTCATCAGGTCCCTTCACTTGAAGCAGGATATCGTTTCCTTTTTTGAGATGATATCCTGTCCAATGCTTGAGTGGCTGGGATACATGGAAACACAGGTTGAGGGTACTAGTGAGCAATTTGAACTCCTATGGACCGAAATGCTTAATTGTTTCAGACGAAGTCAGCCTCCAATAGTCTTTGATTCTGCATTTCTCGAACTCCAGGCCTCCATCCTTGAGAAATCTCTTGATCACTCAAAACTGGCCATTTCAGAGCCAACCATCACCTTTTGGAACTCCACATATGGCGAACTAATCAACTTGGACTACCCCCAGACCTTGCTGCATGTCCTAGACAAGCTTTGGAGAAAGGGAAGAATAAACCTCCACAAGAGAATCCTACCTCTTCAAAGATGTCAGTCTAAATCGGAAGCCATCGCTGCTCCACCAAGATTCACAGTCAATAACACAACACACAATAGAGGCTCCAAAAGAGTAGAACTAGTAGAGAACACCATAGACAGCAAGCAGAAGGAAATGCCACATTCGATTTTGAAAAGGAAAAGGGTGGAACTGACCGAGCATCAGAAGGAAGTAAGACGAGCACAACGAGGAAGGGGGAGGGATTGTGGTGGACACGGCATTGGTATCCAGACTTATACAACACTCGATTTTTCACAAGGGAACGAGGACTCACAAGGGAATGAGGATTCACAAGACAGCGAAGATATCCGGAATCCTGAATGCATTTTGCAGATGTTGAGAAACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04550 map04550
Pfam Domains
Protein Families
Rif1_N

Protein Analysis

1142

Amino Acids

128.91

Weight (kDa)

6.56

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rif1_N PF12231 2 - 69 3.4e-09 Rap1-interacting factor 1 N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1788, 2640
AccB1I GGYRCC 3 cut(s) 53, 547, 2370
AccI GTMKAC 5 cut(s) 464, 932, 1173, 2321, 2384
AccII CGCG 3 cut(s) 209, 423, 425
AccIII TCCGGA 1 cut(s) 3384
AciI CCGC 4 cut(s) 252, 264, 275, 425
AclWI GGATC 6 cut(s) 1199, 1413, 1728, 1741, 2103, 2445
AcoI YGGCCR 1 cut(s) 2916
AcsI RAATTY 5 cut(s) 7, 822, 1553, 1810, 2488
AcuI CTGAAG 3 cut(s) 33, 1631, 1670
AcvI CACGTG 1 cut(s) 1107
AcyI GRCGYC 1 cut(s) 548
AdeI CACNNNGTG 1 cut(s) 1110
AfaI GTAC 4 cut(s) 1872, 1909, 2015, 2779
AfiI CCNNNNNNNGG 2 cut(s) 1052, 1786
AflIII ACRYGT 2 cut(s) 1104, 2399
AhdI GACNNNNNGTC 1 cut(s) 2200
AhlI ACTAGT 3 cut(s) 2387, 2780, 3154
AjnI CCWGG 5 cut(s) 873, 881, 1980, 2029, 2874
AjuI GAANNNNNNNTTGG 2 cut(s) 278, 310
Alw21I GWGCWC 3 cut(s) 24, 152, 3259
Alw26I GTCTC 4 cut(s) 540, 582, 659, 783
AlwI GGATC 6 cut(s) 1199, 1413, 1728, 1741, 2103, 2445
Aor13HI TCCGGA 1 cut(s) 3384
AoxI GGCC 4 cut(s) 1092, 2027, 2877, 2916
ApeKI GCWGC 6 cut(s) 944, 1190, 2034, 2568, 2995, 3092
ApoI RAATTY 5 cut(s) 7, 822, 1553, 1810, 2488
ArsI GACNNNNNNTTYG 6 cut(s) 202, 234, 320, 352, 1166, 1198
AseI ATTAAT 2 cut(s) 807, 1245
AspLEI GCGC 4 cut(s) 423, 425, 550, 2671
AspS9I GGNCC 5 cut(s) 1976, 2027, 2347, 2682, 2805
AsuC2I CCSGG 2 cut(s) 154, 2239
AsuHPI GGTGA 7 cut(s) 902, 1007, 1452, 1696, 1842, 2474, 2929
AsuNHI GCTAGC 1 cut(s) 499
AvaII GGWCC 4 cut(s) 1976, 2347, 2682, 2805
AxyI CCTNAGG 1 cut(s) 2500
BaeGI GKGCMC 1 cut(s) 2188
BalI TGGCCA 1 cut(s) 2918
BamHI GGATCC 1 cut(s) 1733
BanI GGYRCC 3 cut(s) 53, 547, 2370
BanII GRGCYC 2 cut(s) 24, 152
BauI CACGAG 1 cut(s) 1991
BbrPI CACGTG 1 cut(s) 1107
BbsI GAAGAC 2 cut(s) 571, 2630
Bbv12I GWGCWC 3 cut(s) 24, 152, 3259
BbvI GCAGC 6 cut(s) 931, 1202, 2021, 2555, 2982, 3079
BceAI ACGGC 2 cut(s) 913, 3307
BciT130I CCWGG 5 cut(s) 875, 883, 1982, 2031, 2876
BciVI GTATCC 3 cut(s) 1392, 2747, 3311
BclI TGATCA 3 cut(s) 1158, 2539, 2902
BcnI CCSGG 2 cut(s) 154, 2239
BcoDI GTCTC 4 cut(s) 540, 582, 659, 783
BcuI ACTAGT 3 cut(s) 2387, 2780, 3154
BfmI CTRYAG 1 cut(s) 1441
BfoI RGCGCY 2 cut(s) 551, 2672
BfuAI ACCTGC 2 cut(s) 1788, 2640
BfuI GTATCC 3 cut(s) 1392, 2747, 3311
BglII AGATCT 2 cut(s) 626, 2547
BisI GCNGC 7 cut(s) 264, 945, 1191, 2035, 2569, 2996, 3093
BlpI GCTNAGC 2 cut(s) 146, 2253
BlsI GCNGC 7 cut(s) 265, 946, 1192, 2036, 2570, 2997, 3094
Bme1390I CCNGG 7 cut(s) 154, 875, 883, 1982, 2031, 2239, 2876
Bme18I GGWCC 4 cut(s) 1976, 2347, 2682, 2805
BmeRI GACNNNNNGTC 1 cut(s) 2200
BmgT120I GGNCC 5 cut(s) 1976, 2027, 2347, 2682, 2805
BmrFI CCNGG 7 cut(s) 154, 875, 883, 1982, 2031, 2239, 2876
BmsI GCATC 7 cut(s) 180, 693, 889, 1065, 2455, 2614, 3244
BmtI GCTAGC 1 cut(s) 503
BpiI GAAGAC 2 cut(s) 571, 2630
BpmI CTGGAG 1 cut(s) 2858
Bpu1102I GCTNAGC 2 cut(s) 146, 2253
BpuEI CTTGAG 4 cut(s) 1534, 1924, 2762, 2909
BpuMI CCSGG 2 cut(s) 154, 2239
Bsa29I ATCGAT 1 cut(s) 897
BsaAI YACGTR 2 cut(s) 1107, 1301
BsaBI GATNNNNATC 2 cut(s) 2436, 2442
BsaHI GRCGYC 1 cut(s) 548
BsaI GGTCTC 1 cut(s) 783
BsaJI CCNNGG 7 cut(s) 660, 873, 882, 1400, 1707, 1980, 2223
BsaWI WCCGGW 1 cut(s) 3384
BsaXI ACNNNNNCTCC 2 cut(s) 3265, 3295
Bsc4I CCNNNNNNNGG 2 cut(s) 1052, 1786
Bse1I ACTGG 5 cut(s) 720, 1015, 1554, 2493, 2919
Bse21I CCTNAGG 1 cut(s) 2500
Bse3DI GCAATG 1 cut(s) 2165
Bse8I GATNNNNATC 2 cut(s) 2436, 2442
BseAI TCCGGA 1 cut(s) 3384
BseBI CCWGG 5 cut(s) 875, 883, 1982, 2031, 2876
BseCI ATCGAT 1 cut(s) 897
BseDI CCNNGG 7 cut(s) 660, 873, 882, 1400, 1707, 1980, 2223
BseJI GATNNNNATC 2 cut(s) 2436, 2442
BseLI CCNNNNNNNGG 2 cut(s) 1052, 1786
BseMI GCAATG 1 cut(s) 2165
BseMII CTCAG 8 cut(s) 137, 371, 819, 1400, 2112, 2244, 2334, 2514
BseNI ACTGG 5 cut(s) 720, 1015, 1554, 2493, 2919
BsePI GCGCGC 1 cut(s) 421
BseRI GAGGAG 3 cut(s) 390, 633, 1724
BseSI GKGCMC 1 cut(s) 2188
BseXI GCAGC 6 cut(s) 931, 1202, 2021, 2555, 2982, 3079
BseYI CCCAGC 1 cut(s) 2749
Bsh1236I CGCG 3 cut(s) 209, 423, 425
BshFI GGCC 4 cut(s) 1094, 2029, 2879, 2918
BshNI GGYRCC 3 cut(s) 53, 547, 2370
BshVI ATCGAT 1 cut(s) 897
BsiHKAI GWGCWC 3 cut(s) 24, 152, 3259
BsiSI CCGG 3 cut(s) 154, 2239, 3385
BslFI GGGAC 4 cut(s) 1962, 2205, 2269, 2668
BslI CCNNNNNNNGG 2 cut(s) 1052, 1786
BsmAI GTCTC 4 cut(s) 540, 582, 659, 783
BsmFI GGGAC 4 cut(s) 1962, 2205, 2269, 2668
BsmI GAATGC 4 cut(s) 1348, 1606, 1944, 3401
BsnI GGCC 4 cut(s) 1094, 2029, 2879, 2918
Bso31I GGTCTC 1 cut(s) 783
Bsp1286I GDGCHC 4 cut(s) 24, 152, 2188, 3259
Bsp13I TCCGGA 1 cut(s) 3384
Bsp1407I TGTACA 1 cut(s) 2013
Bsp1720I GCTNAGC 2 cut(s) 146, 2253
Bsp19I CCATGG 1 cut(s) 1400
Bsp68I TCGCGA 1 cut(s) 209
BspACI CCGC 4 cut(s) 252, 264, 275, 425
BspANI GGCC 4 cut(s) 1094, 2029, 2879, 2918
BspCNI CTCAG 8 cut(s) 138, 372, 820, 1401, 2113, 2245, 2335, 2513
BspDI ATCGAT 1 cut(s) 897
BspEI TCCGGA 1 cut(s) 3384
BspFNI CGCG 3 cut(s) 209, 423, 425
BspHI TCATGA 1 cut(s) 1038
BspMI ACCTGC 2 cut(s) 1788, 2640
BspOI GCTAGC 1 cut(s) 503
BspPI GGATC 6 cut(s) 1199, 1413, 1728, 1741, 2103, 2445
BspT107I GGYRCC 3 cut(s) 53, 547, 2370
BspTNI GGTCTC 1 cut(s) 783
BsrDI GCAATG 1 cut(s) 2165
BsrGI TGTACA 1 cut(s) 2013
BsrI ACTGG 5 cut(s) 720, 1015, 1554, 2493, 2919
BssECI CCNNGG 7 cut(s) 660, 873, 882, 1400, 1707, 1980, 2223
BssHII GCGCGC 1 cut(s) 421
BssNAI GTATAC 3 cut(s) 933, 2322, 2385
BssNI GRCGYC 1 cut(s) 548
BssSI CACGAG 1 cut(s) 1991
BssT1I CCWWGG 3 cut(s) 660, 1400, 2223
Bst1107I GTATAC 3 cut(s) 933, 2322, 2385
Bst2BI CACGAG 1 cut(s) 1991
Bst2UI CCWGG 5 cut(s) 875, 883, 1982, 2031, 2876
Bst4CI ACNGT 7 cut(s) 108, 992, 1364, 1474, 2313, 2329, 3112
Bst6I CTCTTC 2 cut(s) 52, 3063
BstACI GRCGYC 1 cut(s) 548
BstAPI GCANNNNNTGC 2 cut(s) 260, 712
BstAUI TGTACA 1 cut(s) 2013
BstBAI YACGTR 2 cut(s) 1107, 1301
BstC8I GCNNGC 7 cut(s) 216, 423, 501, 696, 1096, 1316, 3179
BstDEI CTNAG 9 cut(s) 146, 380, 572, 828, 1409, 2121, 2253, 2343, 2500
BstDSI CCRYGG 1 cut(s) 1400
BstFNI CGCG 3 cut(s) 209, 423, 425
BstH2I RGCGCY 2 cut(s) 551, 2672
BstHHI GCGC 4 cut(s) 423, 425, 550, 2671
BstMAI GTCTC 4 cut(s) 540, 582, 659, 783
BstNI CCWGG 5 cut(s) 875, 883, 1982, 2031, 2876
BstNSI RCATGY 2 cut(s) 2403, 3002
BstSCI CCNGG 7 cut(s) 152, 873, 881, 1980, 2029, 2237, 2874
BstSFI CTRYAG 1 cut(s) 1441
BstSLI GKGCMC 1 cut(s) 2188
BstUI CGCG 3 cut(s) 209, 423, 425
BstV1I GCAGC 6 cut(s) 931, 1202, 2021, 2555, 2982, 3079
BstV2I GAAGAC 2 cut(s) 571, 2630
BstX2I RGATCY 5 cut(s) 626, 1405, 1733, 2095, 2547
BstYI RGATCY 5 cut(s) 626, 1405, 1733, 2095, 2547
BstZ17I GTATAC 3 cut(s) 933, 2322, 2385
Bsu15I ATCGAT 1 cut(s) 897
Bsu36I CCTNAGG 1 cut(s) 2500
BsuI GTATCC 3 cut(s) 1392, 2747, 3311
BsuRI GGCC 4 cut(s) 1094, 2029, 2879, 2918
BsuTUI ATCGAT 1 cut(s) 897
BtgI CCRYGG 1 cut(s) 1400
BtgZI GCGATG 1 cut(s) 3073
BtsIMutI CAGTG 4 cut(s) 727, 997, 2309, 2325
BtuMI TCGCGA 1 cut(s) 209
BveI ACCTGC 2 cut(s) 1788, 2640
Cac8I GCNNGC 7 cut(s) 216, 423, 501, 696, 1096, 1316, 3179
CciI TCATGA 1 cut(s) 1038
CfoI GCGC 4 cut(s) 423, 425, 550, 2671
Cfr13I GGNCC 5 cut(s) 1976, 2027, 2347, 2682, 2805
ClaI ATCGAT 1 cut(s) 897
CseI GACGC 1 cut(s) 220
Csp6I GTAC 4 cut(s) 1871, 1908, 2014, 2778
CviQI GTAC 4 cut(s) 1871, 1908, 2014, 2778
DdeI CTNAG 9 cut(s) 146, 380, 572, 828, 1409, 2121, 2253, 2343, 2500
DinI GGCGCC 1 cut(s) 549
DraI TTTAAA 3 cut(s) 681, 1021, 1431
DraIII CACNNNGTG 1 cut(s) 1110
DriI GACNNNNNGTC 1 cut(s) 2200
EaeI YGGCCR 1 cut(s) 2916
Eam1104I CTCTTC 2 cut(s) 52, 3063
Eam1105I GACNNNNNGTC 1 cut(s) 2200
EarI CTCTTC 2 cut(s) 52, 3063
Ecl136II GAGCTC 2 cut(s) 22, 150
Eco130I CCWWGG 3 cut(s) 660, 1400, 2223
Eco147I AGGCCT 1 cut(s) 2879
Eco24I GRGCYC 2 cut(s) 24, 152
Eco31I GGTCTC 1 cut(s) 783
Eco32I GATATC 3 cut(s) 2704, 2727, 3382
Eco47I GGWCC 4 cut(s) 1976, 2347, 2682, 2805
Eco53kI GAGCTC 2 cut(s) 22, 150
Eco57I CTGAAG 3 cut(s) 33, 1631, 1670
Eco72I CACGTG 1 cut(s) 1107
Eco81I CCTNAGG 1 cut(s) 2500
EcoICRI GAGCTC 2 cut(s) 22, 150
EcoO109I RGGNCCY 2 cut(s) 2347, 2682
EcoRI GAATTC 1 cut(s) 1553
EcoRII CCWGG 5 cut(s) 873, 881, 1980, 2029, 2874
EcoRV GATATC 3 cut(s) 2704, 2727, 3382
EcoT14I CCWWGG 3 cut(s) 660, 1400, 2223
EcoT22I ATGCAT 1 cut(s) 3401
EcoT38I GRGCYC 2 cut(s) 24, 152
EgeI GGCGCC 1 cut(s) 549
EheI GGCGCC 1 cut(s) 549
ErhI CCWWGG 3 cut(s) 660, 1400, 2223
FaqI GGGAC 4 cut(s) 1962, 2205, 2269, 2668
FauI CCCGC 1 cut(s) 259
FauNDI CATATG 1 cut(s) 2956
FbaI TGATCA 3 cut(s) 1158, 2539, 2902
FblI GTMKAC 5 cut(s) 464, 932, 1173, 2321, 2384
Fnu4HI GCNGC 7 cut(s) 264, 945, 1191, 2035, 2569, 2996, 3093
FriOI GRGCYC 2 cut(s) 24, 152
Fsp4HI GCNGC 7 cut(s) 264, 945, 1191, 2035, 2569, 2996, 3093
GlaI GCGC 4 cut(s) 422, 424, 549, 2670
GluI GCNGC 7 cut(s) 264, 945, 1191, 2035, 2569, 2996, 3093
GsaI CCCAGC 1 cut(s) 2753
GsuI CTGGAG 1 cut(s) 2858
HaeII RGCGCY 2 cut(s) 551, 2672
HaeIII GGCC 4 cut(s) 1094, 2029, 2879, 2918
HapII CCGG 3 cut(s) 154, 2239, 3385
HgaI GACGC 1 cut(s) 220
HhaI GCGC 4 cut(s) 423, 425, 550, 2671
Hin1I GRCGYC 1 cut(s) 548
Hin6I GCGC 4 cut(s) 421, 423, 548, 2669
HinP1I GCGC 4 cut(s) 421, 423, 548, 2669
HincII GTYRAC 1 cut(s) 465
HindII GTYRAC 1 cut(s) 465
HindIII AAGCTT 2 cut(s) 1900, 3011
HpaII CCGG 3 cut(s) 154, 2239, 3385
HphI GGTGA 7 cut(s) 902, 1007, 1452, 1696, 1842, 2474, 2929
Hpy99I CGWCG 5 cut(s) 119, 171, 179, 197, 214
HpyCH4III ACNGT 7 cut(s) 108, 992, 1364, 1474, 2313, 2329, 3112
HpyCH4IV ACGT 2 cut(s) 1106, 1300
HpyF3I CTNAG 9 cut(s) 146, 380, 572, 828, 1409, 2121, 2253, 2343, 2500
HpySE526I ACGT 2 cut(s) 1106, 1300
Hsp92I GRCGYC 1 cut(s) 548
HspAI GCGC 4 cut(s) 421, 423, 548, 2669
KasI GGCGCC 1 cut(s) 547
Kpn2I TCCGGA 1 cut(s) 3384
Ksp22I TGATCA 3 cut(s) 1158, 2539, 2902
LmnI GCTCC 7 cut(s) 155, 156, 403, 1513, 2183, 3100, 3143
Lsp1109I GCAGC 6 cut(s) 931, 1202, 2021, 2555, 2982, 3079
LweI GCATC 7 cut(s) 180, 693, 889, 1065, 2455, 2614, 3244
MaeII ACGT 2 cut(s) 1106, 1300
MaeIII GTNAC 5 cut(s) 69, 1067, 1119, 1655, 1702
MfeI CAATTG 2 cut(s) 255, 2631
MflI RGATCY 5 cut(s) 626, 1405, 1733, 2095, 2547
MhlI GDGCHC 4 cut(s) 24, 152, 2188, 3259
MlsI TGGCCA 1 cut(s) 2918
MluNI TGGCCA 1 cut(s) 2918
Mly113I GGCGCC 1 cut(s) 548
MlyI GAGTC 5 cut(s) 79, 321, 845, 2111, 3338
MmeI TCCRAC 2 cut(s) 138, 1398
Mox20I TGGCCA 1 cut(s) 2918
Mph1103I ATGCAT 1 cut(s) 3401
MroI TCCGGA 1 cut(s) 3384
MscI TGGCCA 1 cut(s) 2918
MslI CAYNNNNRTG 1 cut(s) 754
Msp20I TGGCCA 1 cut(s) 2918
MspA1I CMGCKG 2 cut(s) 379, 1499
MspI CCGG 3 cut(s) 154, 2239, 3385
MspR9I CCNGG 7 cut(s) 154, 875, 883, 1982, 2031, 2239, 2876
MunI CAATTG 2 cut(s) 255, 2631
Mva1269I GAATGC 4 cut(s) 1348, 1606, 1944, 3401
MvaI CCWGG 5 cut(s) 875, 883, 1982, 2031, 2876
MvnI CGCG 3 cut(s) 209, 423, 425
NarI GGCGCC 1 cut(s) 548
NciI CCSGG 2 cut(s) 154, 2239
NcoI CCATGG 1 cut(s) 1400
NdeI CATATG 1 cut(s) 2956
NheI GCTAGC 1 cut(s) 499
NmeAIII GCCGAG 1 cut(s) 54
NmuCI GTSAC 3 cut(s) 69, 1655, 1702
NruI TCGCGA 1 cut(s) 209
NsiI ATGCAT 1 cut(s) 3401
NspI RCATGY 2 cut(s) 2403, 3002
PacI TTAATTAA 1 cut(s) 807
PagI TCATGA 1 cut(s) 1038
PauI GCGCGC 1 cut(s) 421
PceI AGGCCT 1 cut(s) 2879
PciI ACATGT 1 cut(s) 2399
PcsI WCGNNNNNNNCGW 1 cut(s) 3259
PctI GAATGC 4 cut(s) 1348, 1606, 1944, 3401
PfeI GAWTC 9 cut(s) 130, 299, 386, 1148, 2855, 3048, 3105, 3362, 3388
PflFI GACNNNGTC 1 cut(s) 2832
PfoI TCCNGGA 1 cut(s) 152
PkrI GCNGC 7 cut(s) 265, 946, 1192, 2036, 2570, 2997, 3094
PleI GAGTC 5 cut(s) 79, 320, 845, 2111, 3338
PluTI GGCGCC 1 cut(s) 551
PmaCI CACGTG 1 cut(s) 1107
PmlI CACGTG 1 cut(s) 1107
PpsI GAGTC 5 cut(s) 79, 320, 845, 2111, 3338
Ppu21I YACGTR 2 cut(s) 1107, 1301
PpuMI RGGWCCY 2 cut(s) 2347, 2682
PscI ACATGT 1 cut(s) 2399
PshBI ATTAAT 2 cut(s) 807, 1245
Psp124BI GAGCTC 2 cut(s) 24, 152
Psp5II RGGWCCY 2 cut(s) 2347, 2682
Psp6I CCWGG 5 cut(s) 873, 881, 1980, 2029, 2874
PspCI CACGTG 1 cut(s) 1107
PspFI CCCAGC 1 cut(s) 2749
PspGI CCWGG 5 cut(s) 873, 881, 1980, 2029, 2874
PspPI GGNCC 5 cut(s) 1976, 2027, 2347, 2682, 2805
PspPPI RGGWCCY 2 cut(s) 2347, 2682
PsuI RGATCY 5 cut(s) 626, 1405, 1733, 2095, 2547
PsyI GACNNNGTC 1 cut(s) 2832
PteI GCGCGC 1 cut(s) 421
PvuII CAGCTG 2 cut(s) 379, 1499
RruI TCGCGA 1 cut(s) 209
RsaI GTAC 4 cut(s) 1872, 1909, 2015, 2779
RsaNI GTAC 4 cut(s) 1871, 1908, 2014, 2778
RseI CAYNNNNRTG 1 cut(s) 754
SacI GAGCTC 2 cut(s) 24, 152
SalI GTCGAC 1 cut(s) 463
SatI GCNGC 7 cut(s) 264, 945, 1191, 2035, 2569, 2996, 3093
Sau96I GGNCC 5 cut(s) 1976, 2027, 2347, 2682, 2805
SchI GAGTC 5 cut(s) 79, 321, 845, 2111, 3338
ScrFI CCNGG 7 cut(s) 154, 875, 883, 1982, 2031, 2239, 2876
SduI GDGCHC 4 cut(s) 24, 152, 2188, 3259
SfaNI GCATC 7 cut(s) 180, 693, 889, 1065, 2455, 2614, 3244
SfcI CTRYAG 1 cut(s) 1441
SfoI GGCGCC 1 cut(s) 549
SinI GGWCC 4 cut(s) 1976, 2347, 2682, 2805
SmiMI CAYNNNNRTG 1 cut(s) 754
SmlI CTYRAG 4 cut(s) 1513, 1903, 2741, 2888
SmoI CTYRAG 4 cut(s) 1513, 1903, 2741, 2888
SpeI ACTAGT 3 cut(s) 2387, 2780, 3154
SseBI AGGCCT 1 cut(s) 2879
SsiI CCGC 4 cut(s) 252, 264, 275, 425
SspDI GGCGCC 1 cut(s) 547
SstI GAGCTC 2 cut(s) 24, 152
StuI AGGCCT 1 cut(s) 2879
StyD4I CCNGG 7 cut(s) 152, 873, 881, 1980, 2029, 2237, 2874
StyI CCWWGG 3 cut(s) 660, 1400, 2223
TaaI ACNGT 7 cut(s) 108, 992, 1364, 1474, 2313, 2329, 3112
TaiI ACGT 2 cut(s) 1109, 1303
TaqI TCGA 9 cut(s) 5, 24, 83, 464, 897, 1930, 2868, 3200, 3321
TaqII GACCGA 2 cut(s) 2822, 3245
TatI WGTACW 3 cut(s) 1870, 1907, 2013
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 9 cut(s) 130, 299, 386, 1148, 2855, 3048, 3105, 3362, 3388
TscAI CASTG 4 cut(s) 727, 997, 2316, 2332
TseFI GTSAC 3 cut(s) 69, 1655, 1702
TseI GCWGC 6 cut(s) 944, 1190, 2034, 2568, 2995, 3092
Tsp45I GTSAC 3 cut(s) 69, 1655, 1702
TspGWI ACGGA 2 cut(s) 155, 755
TspRI CASTG 4 cut(s) 727, 997, 2316, 2332
Tth111I GACNNNGTC 1 cut(s) 2832
VpaK11BI GGWCC 4 cut(s) 1976, 2347, 2682, 2805
VspI ATTAAT 2 cut(s) 807, 1245
XapI RAATTY 5 cut(s) 7, 822, 1553, 1810, 2488
XbaI TCTAGA 1 cut(s) 1285
XceI RCATGY 2 cut(s) 2403, 3002
XcmI CCANNNNNNNNNTGG 1 cut(s) 2742
XmiI GTMKAC 5 cut(s) 464, 932, 1173, 2321, 2384
Zsp2I ATGCAT 1 cut(s) 3401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.