MD15G1166600.v1.1

Rap1-interacting factor 1 N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006378
Physical Location & Seq
Reverse (-)
12518603 .. 12523798
5196 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1166600.v1.1.491

Sequence Viewer

Length: 3420 bp
ATGTCCGGATTTTCAGACCAGCTCGAAGAAATAAAATCCCTGATATCTTCAGCCGCCACCAAAGCAAACAAGTCCTTCGCCTACTCCACCCTCTTACACCTCCAGCAGCAATCCTCCGACTGCCACGATTCAATTCAAATGCTTGCGCGGAGCTGCCGGAGCCTCATCCGTCCGACCGTCGCCGATGTTCGGAACAATGACGAAGAAATTGCAACTCAGGCATTGAAATGCTTGGGCTTTATGATATATCATCCGTCCATCGTTGCCGAAATCGCAGCGGATGATGTCAAATTTGTTTTAGATTCACTGGCAAAGCTCATTACAACCACAAAAATGAAGTCTGTTTGCAATTTAGGGGTGTGGTGCATATCTATCCAACAATTGAGTACACCACTTTTAGCTGCCCATTTCCATTCTCTTTTGCTTGCGATTGTTCATGCCATTGACAATCCTATCGGTTCTTTGTCGACTACTTTTGAGGCTATTCAGGCTGTTATGAAGCTAGCAACTTTGTTAAGTGAGAATATGAGAGAGATGTCGCATATATGGGCTCCTCCAGTATATAGAAGACTCCTTAGCTCTGATAAGAGGGAGAGGGATATGTCGGAGAGTTGTTTATTGAACACTAGGTCTACAATATTACCCCCTCCGCTAAATCTTTCCAAGGCAGTTGTCAAAGATTTGAAGCCAAGGTTGCTTACTGGGATGCACCACATGCTAACCAACGGCATGAAGGTTCAAACTATTCAAGCATGGGGATGGTTTATCCGCTTACTGGGATCTCATGCCTTGAAGAACAGGCAGTTAATTAATCAGATGCTAAAAATTCCCGAGCAGACATTTTCAGATCATGACTCACAAGTTCAAATTGCTTCACAAGTTGCCTGGGAAGGTCTTATTGATGCCCTTGTTCACCCTACCATGATATTACCCTTTGAGACAAATGCGACAAAAAAGGACAATGGTATGGAACAAATAGGAACATGCAAGGGAAACGGTAGTGAAATTAAAAAAAATGGATACTTAAAAGGCACAAAGCTCATTATGACACCACTGATAGGCATCATGTCGACTAAATGTGATGTATCTGTTCATTTAGCTTGCTTGAACACGTGGTGTTATTTATTGCATAAACTTGATATCTCTCTCAGTGATTCTTCTGTCATCAGAATGGTACTAGAGCCTGTTGTTGAGGCAATTTTTCAAGTTGATCCTGATAGTAAGAGCATCTGGGCCAGGAATCTGTGCATAGATTTGCTTAATGATTTCATATTGGCAAAATGTAAAGATATAGATTATGACTCACTTAATCAAGTGAACCATCAGTTATTGGGCAAAACCTCTAGGATTGCTCCCTTTGTATCCGGAAGTTTCTCTTGGAAGCAGTATCCCATCAAATGGTTTCCATGGGATCTGAGTCTTTTAGACTTCCATTTAAAGATGATCTACATTCTTATCTGTCAAGCGTTAAGGGAAACTGTCTCCCACGATAACAGGATTTCAGCTGCGGATGCCTCCTTAAGGTTATTTAGATCTGTTTTGAAAGGAGTTCAGTTGGAGTTGAAAAGGTCGTCTATCAGTTATGATGATATTATGTTTTGTTTGAATGCAATATTGAAGTTCGTAAAAAATACTTGTGAAAAAGTTATTTCAGATTGTAGTGACAGAAATGACTTACATCACATATCGCTTCAGTTGGTGGAGGCTGTCTGTGAGGAGATTGAACATACTATAGTGGGATCCCCTCTTTACAGGGTGCCATTTGACATCAAGTGTATTGATAACCTGCAAATATTAGTTGACATTGGATGTGGAAAATCTGGTGTCACTAACGCTTACATGGATATGGTTTCACCAATGGTTTATTTAAGTGTACTTTACTTCTGCTTAGTGGTTCAGTCAACAATGACTGAACCCAAAACAGACAACAATCTTCAGAGAATGCAGAATTATTTCAAATATCTGTTATCAATGTTTGATCCCCTGGAAAGCCTAGTTGTCACTACTGGATTATTGTACAAGCACAGTGGGCCTAGCTGCTTAAGGATGTGGATTGCAGTGGCAGAAGGTTTGAAGTTCTACATTGATGATATGAAGGATTTTTCGTCATTGAAAATGGAGTCTGACAGCAAATGTTGTTTTGCCGTGTGCGATCTGTTGTCCTATCCACTAGTTGTATGCTCCTGTACTCCGAAATACTTTATGTCTGCAGAGCTTGGAAGCCCCTCAAAAGAATCTCATGCTTCACATCACATACAGGTTGAGCTTGACCAAGTTATCACACTCTGGAAGTCCCTCTATTGTTCCCTCTGCACCTCAATATCTGGGTGTTTCATTGGCGGCAGTTTCTTTGAGGATCTCTTTTCAATATTGGATAGGTGCCTTGAAAAGTGTACTAGCATGCTTGTGTGTGCTGATGAGTATGTCTTAAAATTCAAGGATCTGGCTCTTCATTGCATTTCCTCATATGGCGATGTGTTGATATGTATTTTGGAAAAATTCTGCTCTTCAGAATTTAATTCATATCGAGATAACAAGCATGGCTCTAACCACAAGGTCTCTAGTGCCATTCAATTCTGCTTGAAATTGATCATCAGATATATGAAGTTGTTGCAGACAAAGATAGGATCAGACGAACCAATTGGTCTTAGTGCAGTCTCAAGGGTATATTCTGCATTGGCACACTTGCTGAGTTCCCTGCACTTGAAAGAGGATATCCTATCCTTCTTCAAGATAGTATCTGATCCACTGCTTCAGTGGCTGGTACTCATGGAAACACAAGATGAGAACACCAGCAATCAATTTCAACTCCTGTGGGCAGAAACGCTTAATTGTTTAAGGAGGAGTCAGCCTCCAATAATCTTTGACTCTGCATTTCTCCAACTCCAAGCACCCATCCTTGCAAAAACTCTTGATCACTCAAACCTGTCCATTTCAAATCCAACCATCACCTTCTGGAATTCCACATATGGCGAACAAACTGAATTGGATTACCCCCAGACCTTACGTCATGTCTTAGACAAGCTCTGGAGAAATGGAAGAATAAGCCTCCATAAGAGAAGCCCACCACGTCAAAGATGTCAATCAAGACCGGGATCCGCTACCGCTCCACCAAGATACAGGGTCAATGCAACCCATAACAGAGGCTCAAAAAGAGTAGAACTGCAAGAGGACACGATAGGCGGTGAGCTCAAAGAAATGCCTCCATGTTCGAGTTTGAAAAGGAAAAGGTTGGAACTAACTGAGCATCAGAAGGAAGTAAGGCGAGCACAGCAAGGAAGGGAGAGGGATTGTAGCGGTCACGGCCCGGGCATCCAGACTTTCACAAGCATGGATTTTTCGCAGGGGAACGAGGATACGCAAGACAACCAAGATATCCGGAATGCAGAGTGCATCTTGGAGCTGTTGAGAAAGGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04550 map04550
Pfam Domains
Protein Families
Rif1_N

Protein Analysis

1140

Amino Acids

128.61

Weight (kDa)

7.03

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rif1_N PF12231 24 - 303 6e-35 Rap1-interacting factor 1 N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1794
AccB1I GGYRCC 2 cut(s) 1756, 2379
AccB7I CCANNNNNTGG 1 cut(s) 1398
AccBSI CCGCTC 1 cut(s) 3107
AccI GTMKAC 3 cut(s) 467, 632, 1070
AccII CGCG 1 cut(s) 148
AccIII TCCGGA 3 cut(s) 5, 1364, 3378
AcsI RAATTY 6 cut(s) 290, 825, 2432, 2498, 2513, 2959
AcuI CTGAAG 5 cut(s) 33, 1676, 1919, 2493, 2738
AcvI CACGTG 1 cut(s) 1113
AdeI CACNNNGTG 1 cut(s) 1116
AfaI GTAC 7 cut(s) 388, 1176, 1875, 2018, 2188, 2395, 2766
AfiI CCNNNNNNNGG 5 cut(s) 189, 775, 1058, 1398, 1521
AflII CTTAAG 2 cut(s) 1519, 2041
AflIII ACRYGT 1 cut(s) 1110
AhdI GACNNNNNGTC 1 cut(s) 3006
AhlI ACTAGT 1 cut(s) 2170
AjiI CACGTC 1 cut(s) 3071
AjnI CCWGG 3 cut(s) 884, 1235, 1983
AjuI GAANNNNNNNTTGG 2 cut(s) 1360, 1392
Alw21I GWGCWC 2 cut(s) 3192, 3271
Alw26I GTCTC 4 cut(s) 932, 1486, 2563, 2662
AlwNI CAGNNNCTG 1 cut(s) 2761
Ama87I CYCGRG 2 cut(s) 830, 3307
Aor13HI TCCGGA 3 cut(s) 5, 1364, 3378
AoxI GGCC 3 cut(s) 1233, 2030, 3304
ApeKI GCWGC 6 cut(s) 106, 153, 275, 401, 1505, 2037
ApoI RAATTY 6 cut(s) 290, 825, 2432, 2498, 2513, 2959
ArsI GACNNNNNNTTYG 2 cut(s) 323, 355
AseI ATTAAT 1 cut(s) 810
Asp700I GAANNNNTTC 1 cut(s) 1952
AspLEI GCGC 1 cut(s) 148
AspS9I GGNCC 3 cut(s) 1233, 2030, 3305
AsuC2I CCSGG 3 cut(s) 3093, 3308, 3309
AsuHPI GGTGA 4 cut(s) 905, 1845, 2941, 3197
AsuNHI GCTAGC 1 cut(s) 502
AvaI CYCGRG 2 cut(s) 830, 3307
BamHI GGATCC 2 cut(s) 1739, 3095
BanI GGYRCC 2 cut(s) 1756, 2379
BanII GRGCYC 2 cut(s) 553, 3192
BbrPI CACGTG 1 cut(s) 1113
BbsI GAAGAC 1 cut(s) 574
Bbv12I GWGCWC 2 cut(s) 3192, 3271
BbvI GCAGC 6 cut(s) 118, 140, 287, 388, 1492, 2024
BccI CCATC 6 cut(s) 266, 753, 1329, 1400, 2903, 2954
BceAI ACGGC 3 cut(s) 742, 2129, 3319
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BciT130I CCWGG 3 cut(s) 886, 1237, 1985
BciVI GTATCC 4 cut(s) 1013, 1372, 1398, 3349
BclI TGATCA 2 cut(s) 2589, 2914
BcnI CCSGG 3 cut(s) 3093, 3308, 3309
BcoDI GTCTC 4 cut(s) 932, 1486, 2563, 2662
BcuI ACTAGT 1 cut(s) 2170
BfaI CTAG 9 cut(s) 503, 627, 1178, 1344, 1994, 2034, 2171, 2397, 2562
BfmI CTRYAG 2 cut(s) 1731, 2208
BfrI CTTAAG 2 cut(s) 1519, 2041
BfuAI ACCTGC 1 cut(s) 1794
BfuI GTATCC 4 cut(s) 1013, 1372, 1398, 3349
BglII AGATCT 1 cut(s) 1532
BisI GCNGC 8 cut(s) 54, 107, 154, 276, 402, 1506, 2038, 2341
BlsI GCNGC 8 cut(s) 55, 108, 155, 277, 403, 1507, 2039, 2342
Bme1390I CCNGG 6 cut(s) 886, 1237, 1985, 3093, 3308, 3309
BmeRI GACNNNNNGTC 1 cut(s) 3006
BmeT110I CYCGRG 2 cut(s) 830, 3307
BmgBI CACGTC 1 cut(s) 3071
BmgT120I GGNCC 3 cut(s) 1233, 2030, 3305
BmiI GGNNCC 6 cut(s) 161, 552, 1741, 1758, 2381, 3097
BmrFI CCNGG 6 cut(s) 886, 1237, 1985, 3093, 3308, 3309
BmrI ACTGGG 2 cut(s) 711, 785
BmsI GCATC 9 cut(s) 696, 807, 892, 1071, 1236, 1501, 3256, 3321, 3402
BmtI GCTAGC 1 cut(s) 506
BmuI ACTGGG 2 cut(s) 711, 785
BpiI GAAGAC 1 cut(s) 574
BplI GAGNNNNNCTC 2 cut(s) 2836, 2868
BpmI CTGGAG 3 cut(s) 86, 540, 3049
Bpu10I CCTNAGC 1 cut(s) 575
BpuEI CTTGAG 1 cut(s) 2644
BpuMI CCSGG 3 cut(s) 3093, 3308, 3309
BsaAI YACGTR 1 cut(s) 1113
BsaBI GATNNNNATC 2 cut(s) 1061, 3082
BsaI GGTCTC 1 cut(s) 2563
BsaJI CCNNGG 6 cut(s) 663, 689, 885, 1406, 1983, 3307
BsaWI WCCGGW 3 cut(s) 5, 1364, 3378
Bsc4I CCNNNNNNNGG 5 cut(s) 189, 775, 1058, 1398, 1521
Bse1I ACTGG 5 cut(s) 312, 557, 706, 780, 2011
Bse3DI GCAATG 1 cut(s) 2452
Bse8I GATNNNNATC 2 cut(s) 1061, 3082
BseAI TCCGGA 3 cut(s) 5, 1364, 3378
BseBI CCWGG 3 cut(s) 886, 1237, 1985
BseDI CCNNGG 6 cut(s) 663, 689, 885, 1406, 1983, 3307
BseJI GATNNNNATC 2 cut(s) 1061, 3082
BseLI CCNNNNNNNGG 5 cut(s) 189, 775, 1058, 1398, 1521
BseMI GCAATG 1 cut(s) 2452
BseMII CTCAG 5 cut(s) 230, 1162, 1406, 2681, 3234
BseNI ACTGG 5 cut(s) 312, 557, 706, 780, 2011
BseRI GAGGAG 3 cut(s) 543, 1730, 2857
BseXI GCAGC 6 cut(s) 118, 140, 287, 388, 1492, 2024
BsgI GTGCAG 3 cut(s) 2296, 2673, 2684
Bsh1236I CGCG 1 cut(s) 148
Bsh1285I CGRYCG 1 cut(s) 177
BshFI GGCC 3 cut(s) 1235, 2032, 3306
BshNI GGYRCC 2 cut(s) 1756, 2379
BsiEI CGRYCG 1 cut(s) 177
BsiHKAI GWGCWC 2 cut(s) 3192, 3271
BsiHKCI CYCGRG 2 cut(s) 830, 3307
BsiSI CCGG 6 cut(s) 6, 157, 1365, 3092, 3308, 3379
BslFI GGGAC 1 cut(s) 2278
BslI CCNNNNNNNGG 5 cut(s) 189, 775, 1058, 1398, 1521
BsmAI GTCTC 4 cut(s) 932, 1486, 2563, 2662
BsmFI GGGAC 1 cut(s) 2278
BsmI GAATGC 3 cut(s) 1612, 1947, 3388
BsnI GGCC 3 cut(s) 1235, 2032, 3306
Bso31I GGTCTC 1 cut(s) 2563
BsoBI CYCGRG 2 cut(s) 830, 3307
Bsp1286I GDGCHC 3 cut(s) 553, 3192, 3271
Bsp13I TCCGGA 3 cut(s) 5, 1364, 3378
Bsp1407I TGTACA 1 cut(s) 2016
Bsp19I CCATGG 1 cut(s) 1406
BspANI GGCC 3 cut(s) 1235, 2032, 3306
BspCNI CTCAG 5 cut(s) 229, 1161, 1407, 2682, 3235
BspEI TCCGGA 3 cut(s) 5, 1364, 3378
BspFNI CGCG 1 cut(s) 148
BspHI TCATGA 1 cut(s) 850
BspLI GGNNCC 6 cut(s) 161, 552, 1741, 1758, 2381, 3097
BspMAI CTGCAG 1 cut(s) 2212
BspMI ACCTGC 1 cut(s) 1794
BspOI GCTAGC 1 cut(s) 506
BspQI GCTCTTC 2 cut(s) 2454, 2512
BspT107I GGYRCC 2 cut(s) 1756, 2379
BspTI CTTAAG 2 cut(s) 1519, 2041
BspTNI GGTCTC 1 cut(s) 2563
BsrBI CCGCTC 1 cut(s) 3107
BsrDI GCAATG 1 cut(s) 2452
BsrGI TGTACA 1 cut(s) 2016
BsrI ACTGG 5 cut(s) 312, 557, 706, 780, 2011
BssECI CCNNGG 6 cut(s) 663, 689, 885, 1406, 1983, 3307
BssT1I CCWWGG 3 cut(s) 663, 689, 1406
Bst2UI CCWGG 3 cut(s) 886, 1237, 1985
Bst4CI ACNGT 4 cut(s) 178, 998, 1480, 2027
Bst6I CTCTTC 2 cut(s) 2454, 2512
BstAFI CTTAAG 2 cut(s) 1519, 2041
BstAPI GCANNNNNTGC 1 cut(s) 715
BstAUI TGTACA 1 cut(s) 2016
BstBAI YACGTR 1 cut(s) 1113
BstC8I GCNNGC 6 cut(s) 144, 426, 504, 1102, 2402, 3267
BstDEI CTNAG 9 cut(s) 216, 575, 1148, 1415, 1888, 2648, 2690, 3016, 3243
BstDSI CCRYGG 1 cut(s) 1406
BstENI CCTNNNNNAGG 1 cut(s) 1519
BstFNI CGCG 1 cut(s) 148
BstHHI GCGC 1 cut(s) 148
BstMAI GTCTC 4 cut(s) 932, 1486, 2563, 2662
BstMCI CGRYCG 1 cut(s) 177
BstNI CCWGG 3 cut(s) 886, 1237, 1985
BstNSI RCATGY 3 cut(s) 718, 987, 2404
BstSCI CCNGG 6 cut(s) 884, 1235, 1983, 3091, 3306, 3307
BstSFI CTRYAG 2 cut(s) 1731, 2208
BstUI CGCG 1 cut(s) 148
BstV1I GCAGC 6 cut(s) 118, 140, 287, 388, 1492, 2024
BstV2I GAAGAC 1 cut(s) 574
BstX2I RGATCY 7 cut(s) 779, 1411, 1532, 1739, 2356, 2440, 3095
BstYI RGATCY 7 cut(s) 779, 1411, 1532, 1739, 2356, 2440, 3095
BsuI GTATCC 4 cut(s) 1013, 1372, 1398, 3349
BsuRI GGCC 3 cut(s) 1235, 2032, 3306
BtgI CCRYGG 1 cut(s) 1406
BtgZI GCGATG 1 cut(s) 2487
BtrI CACGTC 1 cut(s) 3071
BtsI GCAGTG 2 cut(s) 2064, 2747
BtsIMutI CAGTG 7 cut(s) 305, 1052, 1156, 2032, 2064, 2747, 2762
BveI ACCTGC 1 cut(s) 1794
Cac8I GCNNGC 6 cut(s) 144, 426, 504, 1102, 2402, 3267
CaiI CAGNNNCTG 1 cut(s) 2761
CciI TCATGA 1 cut(s) 850
CfoI GCGC 1 cut(s) 148
Cfr13I GGNCC 3 cut(s) 1233, 2030, 3305
Cfr9I CCCGGG 1 cut(s) 3307
Csp6I GTAC 7 cut(s) 387, 1175, 1874, 2017, 2187, 2394, 2765
CspCI CAANNNNNGTGG 4 cut(s) 2008, 2043, 2795, 2830
CviQI GTAC 7 cut(s) 387, 1175, 1874, 2017, 2187, 2394, 2765
DdeI CTNAG 9 cut(s) 216, 575, 1148, 1415, 1888, 2648, 2690, 3016, 3243
DraI TTTAAA 1 cut(s) 1437
DraIII CACNNNGTG 1 cut(s) 1116
DriI GACNNNNNGTC 1 cut(s) 3006
Eam1104I CTCTTC 2 cut(s) 2454, 2512
Eam1105I GACNNNNNGTC 1 cut(s) 3006
EarI CTCTTC 2 cut(s) 2454, 2512
Ecl136II GAGCTC 1 cut(s) 3190
Eco130I CCWWGG 3 cut(s) 663, 689, 1406
Eco24I GRGCYC 2 cut(s) 553, 3192
Eco31I GGTCTC 1 cut(s) 2563
Eco32I GATATC 4 cut(s) 45, 1141, 2716, 3376
Eco53kI GAGCTC 1 cut(s) 3190
Eco57I CTGAAG 5 cut(s) 33, 1676, 1919, 2493, 2738
Eco72I CACGTG 1 cut(s) 1113
Eco88I CYCGRG 2 cut(s) 830, 3307
EcoICRI GAGCTC 1 cut(s) 3190
EcoNI CCTNNNNNAGG 1 cut(s) 1519
EcoRI GAATTC 1 cut(s) 2959
EcoRII CCWGG 3 cut(s) 884, 1235, 1983
EcoRV GATATC 4 cut(s) 45, 1141, 2716, 3376
EcoT14I CCWWGG 3 cut(s) 663, 689, 1406
EcoT38I GRGCYC 2 cut(s) 553, 3192
ErhI CCWWGG 3 cut(s) 663, 689, 1406
FalI AAGNNNNNCTT 2 cut(s) 1360, 1392
FaqI GGGAC 1 cut(s) 2278
FauNDI CATATG 2 cut(s) 2467, 2968
FbaI TGATCA 2 cut(s) 2589, 2914
FblI GTMKAC 3 cut(s) 467, 632, 1070
Fnu4HI GCNGC 8 cut(s) 54, 107, 154, 276, 402, 1506, 2038, 2341
FriOI GRGCYC 2 cut(s) 553, 3192
Fsp4HI GCNGC 8 cut(s) 54, 107, 154, 276, 402, 1506, 2038, 2341
FspBI CTAG 9 cut(s) 503, 627, 1178, 1344, 1994, 2034, 2171, 2397, 2562
GlaI GCGC 1 cut(s) 147
GluI GCNGC 8 cut(s) 54, 107, 154, 276, 402, 1506, 2038, 2341
GsuI CTGGAG 3 cut(s) 86, 540, 3049
HaeIII GGCC 3 cut(s) 1235, 2032, 3306
HapII CCGG 6 cut(s) 6, 157, 1365, 3092, 3308, 3379
HhaI GCGC 1 cut(s) 148
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HincII GTYRAC 4 cut(s) 468, 1071, 1801, 1902
HindII GTYRAC 4 cut(s) 468, 1071, 1801, 1902
HpaII CCGG 6 cut(s) 6, 157, 1365, 3092, 3308, 3379
HphI GGTGA 4 cut(s) 905, 1845, 2941, 3197
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 9 cut(s) 85, 727, 884, 2060, 2089, 2734, 2962, 3247, 3273
HpyCH4III ACNGT 4 cut(s) 178, 998, 1480, 2027
HpyCH4IV ACGT 3 cut(s) 1112, 3007, 3070
HpyF3I CTNAG 9 cut(s) 216, 575, 1148, 1415, 1888, 2648, 2690, 3016, 3243
HpySE526I ACGT 3 cut(s) 1112, 3007, 3070
HspAI GCGC 1 cut(s) 146
Kpn2I TCCGGA 3 cut(s) 5, 1364, 3378
Ksp22I TGATCA 2 cut(s) 2589, 2914
LguI GCTCTTC 2 cut(s) 2454, 2512
LmnI GCTCC 7 cut(s) 150, 159, 556, 1357, 2186, 3112, 3400
Lsp1109I GCAGC 6 cut(s) 118, 140, 287, 388, 1492, 2024
LweI GCATC 9 cut(s) 696, 807, 892, 1071, 1236, 1501, 3256, 3321, 3402
MaeI CTAG 9 cut(s) 503, 627, 1178, 1344, 1994, 2034, 2171, 2397, 2562
MaeII ACGT 3 cut(s) 1112, 3007, 3070
MaeIII GTNAC 4 cut(s) 1661, 1825, 1999, 3299
MbiI CCGCTC 1 cut(s) 3107
MfeI CAATTG 2 cut(s) 380, 2640
MflI RGATCY 7 cut(s) 779, 1411, 1532, 1739, 2356, 2440, 3095
MhlI GDGCHC 3 cut(s) 553, 3192, 3271
MlyI GAGTC 7 cut(s) 564, 848, 1295, 1426, 2129, 2854, 2861
MmeI TCCRAC 8 cut(s) 141, 197, 400, 585, 1536, 2905, 2966, 3213
MroI TCCGGA 3 cut(s) 5, 1364, 3378
MroXI GAANNNNTTC 1 cut(s) 1952
MslI CAYNNNNRTG 7 cut(s) 226, 332, 757, 1169, 1844, 2405, 3329
MspA1I CMGCKG 2 cut(s) 278, 1505
MspCI CTTAAG 2 cut(s) 1519, 2041
MspI CCGG 6 cut(s) 6, 157, 1365, 3092, 3308, 3379
MspR9I CCNGG 6 cut(s) 886, 1237, 1985, 3093, 3308, 3309
MunI CAATTG 2 cut(s) 380, 2640
Mva1269I GAATGC 3 cut(s) 1612, 1947, 3388
MvaI CCWGG 3 cut(s) 886, 1237, 1985
MvnI CGCG 1 cut(s) 148
NciI CCSGG 3 cut(s) 3093, 3308, 3309
NcoI CCATGG 1 cut(s) 1406
NdeI CATATG 2 cut(s) 2467, 2968
NheI GCTAGC 1 cut(s) 502
NlaIV GGNNCC 6 cut(s) 161, 552, 1741, 1758, 2381, 3097
NmuCI GTSAC 4 cut(s) 1661, 1825, 1999, 3299
NspI RCATGY 3 cut(s) 718, 987, 2404
PacI TTAATTAA 1 cut(s) 810
PaeI GCATGC 1 cut(s) 2404
PagI TCATGA 1 cut(s) 850
PciSI GCTCTTC 2 cut(s) 2454, 2512
PctI GAATGC 3 cut(s) 1612, 1947, 3388
PdmI GAANNNNTTC 1 cut(s) 1952
PfeI GAWTC 5 cut(s) 128, 302, 1154, 1240, 2234
PflMI CCANNNNNTGG 1 cut(s) 1398
PkrI GCNGC 8 cut(s) 55, 108, 155, 277, 403, 1507, 2039, 2342
PleI GAGTC 7 cut(s) 564, 848, 1295, 1425, 2128, 2853, 2861
PmaCI CACGTG 1 cut(s) 1113
PmlI CACGTG 1 cut(s) 1113
PpsI GAGTC 7 cut(s) 564, 848, 1295, 1425, 2128, 2853, 2861
Ppu21I YACGTR 1 cut(s) 1113
PshBI ATTAAT 1 cut(s) 810
Psp124BI GAGCTC 1 cut(s) 3192
Psp6I CCWGG 3 cut(s) 884, 1235, 1983
PspCI CACGTG 1 cut(s) 1113
PspGI CCWGG 3 cut(s) 884, 1235, 1983
PspN4I GGNNCC 6 cut(s) 161, 552, 1741, 1758, 2381, 3097
PspPI GGNCC 3 cut(s) 1233, 2030, 3305
PstI CTGCAG 1 cut(s) 2212
PstNI CAGNNNCTG 1 cut(s) 2761
PsuI RGATCY 7 cut(s) 779, 1411, 1532, 1739, 2356, 2440, 3095
PvuII CAGCTG 1 cut(s) 1505
RsaI GTAC 7 cut(s) 388, 1176, 1875, 2018, 2188, 2395, 2766
RsaNI GTAC 7 cut(s) 387, 1175, 1874, 2017, 2187, 2394, 2765
RseI CAYNNNNRTG 7 cut(s) 226, 332, 757, 1169, 1844, 2405, 3329
SacI GAGCTC 1 cut(s) 3192
SalI GTCGAC 2 cut(s) 466, 1069
SapI GCTCTTC 2 cut(s) 2454, 2512
SatI GCNGC 8 cut(s) 54, 107, 154, 276, 402, 1506, 2038, 2341
Sau96I GGNCC 3 cut(s) 1233, 2030, 3305
SchI GAGTC 7 cut(s) 564, 848, 1295, 1426, 2129, 2854, 2861
ScrFI CCNGG 6 cut(s) 886, 1237, 1985, 3093, 3308, 3309
SduI GDGCHC 3 cut(s) 553, 3192, 3271
SfaNI GCATC 9 cut(s) 696, 807, 892, 1071, 1236, 1501, 3256, 3321, 3402
SfcI CTRYAG 2 cut(s) 1731, 2208
SmaI CCCGGG 1 cut(s) 3309
SmiMI CAYNNNNRTG 7 cut(s) 226, 332, 757, 1169, 1844, 2405, 3329
SmlI CTYRAG 3 cut(s) 1519, 2041, 2659
SmoI CTYRAG 3 cut(s) 1519, 2041, 2659
SpeI ACTAGT 1 cut(s) 2170
SphI GCATGC 1 cut(s) 2404
SrfI GCCCGGGC 1 cut(s) 3309
SspI AATATT 4 cut(s) 639, 1614, 1794, 2370
SspMI CTAG 9 cut(s) 503, 627, 1178, 1344, 1994, 2034, 2171, 2397, 2562
SstI GAGCTC 1 cut(s) 3192
StyD4I CCNGG 6 cut(s) 884, 1235, 1983, 3091, 3306, 3307
StyI CCWWGG 3 cut(s) 663, 689, 1406
TaaI ACNGT 4 cut(s) 178, 998, 1480, 2027
TaiI ACGT 3 cut(s) 1115, 3010, 3073
TaqI TCGA 5 cut(s) 24, 467, 1070, 2527, 3212
TatI WGTACW 5 cut(s) 386, 1873, 2016, 2186, 2393
TauI GCSGC 2 cut(s) 56, 2343
TfiI GAWTC 5 cut(s) 128, 302, 1154, 1240, 2234
TscAI CASTG 7 cut(s) 312, 1059, 1156, 2032, 2064, 2754, 2762
TseFI GTSAC 4 cut(s) 1661, 1825, 1999, 3299
TseI GCWGC 6 cut(s) 106, 153, 275, 401, 1505, 2037
Tsp45I GTSAC 4 cut(s) 1661, 1825, 1999, 3299
TspGWI ACGGA 2 cut(s) 158, 243
TspMI CCCGGG 1 cut(s) 3307
TspRI CASTG 7 cut(s) 312, 1059, 1156, 2032, 2064, 2754, 2762
Van91I CCANNNNNTGG 1 cut(s) 1398
Vha464I CTTAAG 2 cut(s) 1519, 2041
VspI ATTAAT 1 cut(s) 810
XagI CCTNNNNNAGG 1 cut(s) 1519
XapI RAATTY 6 cut(s) 290, 825, 2432, 2498, 2513, 2959
XceI RCATGY 3 cut(s) 718, 987, 2404
XcmI CCANNNNNNNNNTGG 1 cut(s) 2754
XmaI CCCGGG 1 cut(s) 3307
XmiI GTMKAC 3 cut(s) 467, 632, 1070
XmnI GAANNNNTTC 1 cut(s) 1952
XspI CTAG 9 cut(s) 503, 627, 1178, 1344, 1994, 2034, 2171, 2397, 2562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.