Rh2DG030800

Rap1-interacting factor 1 N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0006378
Physical Location & Seq
Reverse (-)
1895158 .. 1901339
6182 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG030800.1

Sequence Viewer

Length: 3414 bp
ATGTCGAATCTCTCAGACCAGCTCCAAGAAATCAAATCCCTAATTTCTTCACCCACCAAATCAAACAAGTCACTCGCTTACTCCACTCTATCACACCTTCAACAACAGTCCTCCGATGCTCTGATTCAAACACTCGCGCTGAGCTCTCAGAGCCTTCTCCGTTGGATCGTCGCCGATATTCAAGACGACGACGAAGAAATTGCGGCGCAGGCATTGAAATGTTTGGGTTTTATGATATACCATCCGGCCATTGTTTCCGAAATTTCAGTGGAGGATGTGAAGTTGGTTTTGGAATCATTGGTTAAACTCATTACAACTACGAAAATTAAGTCGGTTTGTAATTTGGGAGTGTGGTGCATATCTATACAACAGCTGAGTGAACCACTTTTGGCTCCCCATTTCCATTCTCTTTTGCGCGCAGTAGTTCATGCCATTGACAATCCCATTGGTTCTCTGTCAACAACTTTTGAGGCTATTCAGGCTGTCATTAAGCTAGCTACTCTTTTAAGTGAGAGCATGAGAGAGTTGTCTCATATATGGGCACCTCCAATATATAGGAGACTGCTTAGCTCTGATAAGAGAGACAGGGATATGTCAGAAAGGTGTCTGTTGAAGATTAGATCCACAATCATACCCCCTCCAATAGATCTCTCCAAGGTACTTGTCAGAGATTTAAAGACAAAGTTGCTCACTATGATGCACGATATGCTAACCAGTGGTATGAAGGTTCAAACTATTCAAGCATGGGGATGGTATATCCGCTTACTAGGGTCTCATGCCTTGAAGAACAGACATTTAATTAATGATATGCTGAAAATTCCTGAGAAAACATTTTCAGATCACGACTCACAAGTTCAGATTGCTTCCCAGGTTGCCTGGGAAGGTCTTATTGATGCTCTTATTCATCCTCCAATGATATTACCATGTGCAACAAATGCTGCAAAAGAGGATGATGGTACGCAAAAAAAGGGAACACATAAAGGAAACAGTGGTGACACCCAAAACACTGGATCTTTGAAAAGCGTAAGGCTGATCATGACACCACTGATAGGCATCATGTTGAGTGACTGTGATGTATCTGTCCACATGGCTTGCTTGAACACATGGTGCTATCTATTGCATAGACTTGATACCTCTCTGAATGATTCTTCAATGATCAACCTGGTAGTAGACCCTATCTTTGAAGCAGCTTTTCAACTTGATCCTGATGGTAAGAGCTTCTGGGCTAGGGATCTGTGCATTAATTTGCTTGATGATTTTATATCAGCAAAATGTAAACCTCAAGGCTATGACCCACGTAACCAGGTAAGCCAGCAGTTATCAGCCAAAACCTCTATGAATGCTCCCATTATATCTGGCAGTTGCTCATGGAAGCAGTATCCTATTAAATGGTTGCCATGGGATTTAAGTCTGTTGGACTTTCATTTAAAGATGATCTACAGTCTCATCCGTCAACCATCAAGGCAAACTGTCTGCCATGATGACAGGGTTCCAGCTGATGATGCTCCCTTAAGGTTATTTAGAGCTGTTTTGAAAGGAGTTGAACAGGAGTTCAAGAGGTCGTCCATCGATTATGATGATATTATGTTGTGTTTGAATGCAGTATTGAAGTTCTTAAGAAATATGTGTGAAGAACTAAGTCTGGATGGTAGTGACAGAAATGGCTTGCATCATGTATCATTTCAGTATATGGAGGCTGTCAGCGAGGTGATAGAACCTACTCTAATGGGATCCCCTCTTTACAAGGTGGCATTGAACATCAAGTATATTGAAAACCTGCAAATGATTCCTGACGTTGGATATGAAAAATCAGGTGCCTGCTCTATTGGTTATATGGAAATGGTTTCGCCAATGGTTTATTTAAGCGTACTTTATTTCACTGTGGTGGTTCGGTCAACTTTTAGTACGTCCAAAGCTGACTACATTCTGCAGGGAATGCAGCAATATTTTAAATTTATGTTATCATCTTTTGGTCCCCTGGAAAGTATCGTTATCACAACTGAATTATTGTACAAGCACACAGGGCCAGGCTGCTTAACGATGTGGATAGCAATAGCAGAAAGTCTGAAAAACTACATAAATGATGTGAAGGATCTTTCATTGTTCAAAATGGACTCTGAGAGTAAGGGTTGTTATGCCATGAGCCATCTAATGTCCTATCCACTGCTTGTATGCTCCTGTGCCCAGAAAGACATTATGTCAGCAATGAGTGGATGCTCCTTAGAAACCCCCTCTTCTCCACAGATGAATACTGAGCTTGAACAAGTTATCACATTATGGAAGTCCCTCTATGGTTCTATCTGTACCTCACTGTCTGGGTGTATCATTGTTGGCAGTTTCTCTGAGGCTTTAGTTTCTGTATTGGACAGGCGCCTTGACAAGTATACTAGCATGCTTACATGTACTAACGAGCTTCACTTAAACTTCAAGGATTTGGATCTTCATCTCATTTCCTTATATGGAGATGCTTTTATGTGTATTCTGAAAAATTCCAGTTCTTCAGAATCAAGTTCTGATGGAAATACCAAGCTTTACTATGATTACAAGATCACTAGTGGCATTAAAGGCTGCTTGACTTTGGCCATTAGATATATGAAGCTGTCGCAGACAAAGATAGGAACAGACGCACCAATTGGTCTTCCTATGTTCTCTAGTTGCAGGTTATGTTCTGCATTGGCACTCTTCATCAGGTCCCTTCACTTGAAGCAAGATATTGTTTCCTTCTTTGAGATGATATCATGTCCACTGCTTGAGTGGCTGGGATACAAGAAAATGCAAGATGAGGGCACCAGTGATCAATTTGAACTCCTATGGACCGAAATGCTTAATTGTTTAAGACGAAGTCAGCCTCCAATAGTCTTTGATTCTGCATTTCTCAAATTCCAGGCCTCTATCCTTGAGAAAACTCTTGACCACTCAAAACTGTCCATTTCAGAGCCAACCATCACCTTTTGGAACTCCATATATGGCGAACAGATCAACTTGGATTACCCTCAGACCTTGCTCCATGTCCTGGACAAGCTCTGGAGACAGGGAAGAATAAACCTCCACAAGAGAAGCCTACCTCTTCAAAGATGTCAATCTAAATCGGAAGCCATCACTGCTCCACCGAGATTCACGGTCAATACAGCACACAATAGAGGCTCTAAAAGAGTAGAACTAGTAGAGAATACCACAGACAGTAAGCAGAAGGAAATGCCACATTCGAGTTTGAAAAGGAAGAGGTCGGAACTCACTGAGCATCAGAAGGAAGTGAGACGAGCACAACGAGGAAGGGGAAGGGATTGTGGCGGACACGGCATTGGTATCCAGACTTACACAACACTCGACTTTTCACAAGGGAATGAGGATTCGCAAGACAGCGAAGATATCCGGAATCCAGAATGCATTTTGCAGATGTTGAGGAACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04550 map04550
Pfam Domains
Protein Families
Rif1_N

Protein Analysis

1137

Amino Acids

128.18

Weight (kDa)

6.59

Isoelectric Point (pI)

47.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rif1_N PF12231 19 - 301 1.2e-32 Rap1-interacting factor 1 N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1785, 2649
AccB1I GGYRCC 4 cut(s) 541, 1814, 2370, 2786
AccB7I CCANNNNNTGG 1 cut(s) 3013
AccI GTMKAC 2 cut(s) 1170, 2384
AccII CGCG 2 cut(s) 137, 417
AccIII TCCGGA 1 cut(s) 3372
AciI CCGC 3 cut(s) 203, 760, 3291
AclWI GGATC 9 cut(s) 173, 615, 1018, 1196, 1239, 1725, 1738, 2100, 2445
AcoI YGGCCR 2 cut(s) 246, 2580
AcsI RAATTY 5 cut(s) 261, 816, 1952, 2488, 2879
AcuI CTGAAG 1 cut(s) 2484
AcyI GRCGYC 1 cut(s) 2371
AdeI CACNNNGTG 1 cut(s) 1107
AfaI GTAC 7 cut(s) 660, 958, 1869, 1906, 2012, 2305, 2404
AfiI CCNNNNNNNGG 3 cut(s) 1049, 1796, 3013
AflII CTTAAG 2 cut(s) 1510, 1615
AflIII ACRYGT 1 cut(s) 2399
AhdI GACNNNNNGTC 1 cut(s) 2197
AhlI ACTAGT 2 cut(s) 2552, 3160
AjnI CCWGG 8 cut(s) 867, 875, 1161, 1302, 1977, 2026, 2883, 3012
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
AleI CACNNNNGTG 1 cut(s) 1883
Alw21I GWGCWC 2 cut(s) 146, 3265
Alw26I GTCTC 7 cut(s) 534, 553, 576, 777, 1448, 3022, 3250
AlwI GGATC 9 cut(s) 173, 615, 1018, 1196, 1239, 1725, 1738, 2100, 2445
Aor13HI TCCGGA 1 cut(s) 3372
AoxI GGCC 4 cut(s) 246, 2024, 2580, 2886
ApeKI GCWGC 5 cut(s) 938, 1187, 1939, 2031, 2568
ApoI RAATTY 5 cut(s) 261, 816, 1952, 2488, 2879
ArsI GACNNNNNNTTYG 4 cut(s) 314, 346, 1163, 1195
AseI ATTAAT 2 cut(s) 801, 1242
AspLEI GCGC 5 cut(s) 139, 208, 417, 419, 2373
AspS9I GGNCC 4 cut(s) 1973, 2024, 2691, 2814
AsuHPI GGTGA 4 cut(s) 42, 1004, 1720, 2938
AsuNHI GCTAGC 1 cut(s) 493
AvaII GGWCC 3 cut(s) 1973, 2691, 2814
BaeGI GKGCMC 3 cut(s) 544, 2185, 2789
BalI TGGCCA 1 cut(s) 2582
BamHI GGATCC 1 cut(s) 1730
BanI GGYRCC 4 cut(s) 541, 1814, 2370, 2786
BanII GRGCYC 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 2630
Bbv12I GWGCWC 2 cut(s) 146, 3265
BbvI GCAGC 5 cut(s) 925, 1199, 1951, 2018, 2555
BceAI ACGGC 1 cut(s) 3313
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BciT130I CCWGG 8 cut(s) 869, 877, 1163, 1304, 1979, 2028, 2885, 3014
BciVI GTATCC 3 cut(s) 1389, 2756, 3317
BclI TGATCA 3 cut(s) 1032, 1155, 2794
BcoDI GTCTC 7 cut(s) 534, 553, 576, 777, 1448, 3022, 3250
BcuI ACTAGT 2 cut(s) 2552, 3160
BfaI CTAG 7 cut(s) 494, 767, 1227, 2388, 2553, 2652, 3161
BfmI CTRYAG 2 cut(s) 1438, 1928
BfoI RGCGCY 1 cut(s) 2374
BfrI CTTAAG 2 cut(s) 1510, 1615
BfuAI ACCTGC 2 cut(s) 1785, 2649
BfuI GTATCC 3 cut(s) 1389, 2756, 3317
BglII AGATCT 1 cut(s) 646
BisI GCNGC 6 cut(s) 204, 939, 1188, 1940, 2032, 2569
BlpI GCTNAGC 2 cut(s) 140, 566
BlsI GCNGC 6 cut(s) 205, 940, 1189, 1941, 2033, 2570
Bme1390I CCNGG 8 cut(s) 869, 877, 1163, 1304, 1979, 2028, 2885, 3014
Bme18I GGWCC 3 cut(s) 1973, 2691, 2814
BmeRI GACNNNNNGTC 1 cut(s) 2197
BmgT120I GGNCC 4 cut(s) 1973, 2024, 2691, 2814
BmiI GGNNCC 9 cut(s) 393, 543, 1491, 1732, 1816, 1975, 2372, 2693, 2788
BmrFI CCNGG 8 cut(s) 869, 877, 1163, 1304, 1979, 2028, 2885, 3014
BmsI GCATC 9 cut(s) 106, 687, 883, 1062, 1492, 1678, 2204, 2455, 3250
BmtI GCTAGC 1 cut(s) 497
BpiI GAAGAC 1 cut(s) 2630
BpmI CTGGAG 1 cut(s) 3046
Bpu1102I GCTNAGC 2 cut(s) 140, 566
BpuEI CTTGAG 3 cut(s) 1266, 2771, 2918
Bsa29I ATCGAT 1 cut(s) 1569
BsaAI YACGTR 1 cut(s) 1298
BsaBI GATNNNNATC 4 cut(s) 1052, 2436, 2442, 3079
BsaHI GRCGYC 1 cut(s) 2371
BsaI GGTCTC 1 cut(s) 777
BsaJI CCNNGG 5 cut(s) 654, 867, 876, 1397, 1977
BsaWI WCCGGW 1 cut(s) 3372
Bsc4I CCNNNNNNNGG 3 cut(s) 1049, 1796, 3013
Bse1I ACTGG 4 cut(s) 714, 1012, 2493, 2790
Bse3DI GCAATG 1 cut(s) 2211
Bse8I GATNNNNATC 4 cut(s) 1052, 2436, 2442, 3079
BseAI TCCGGA 1 cut(s) 3372
BseBI CCWGG 8 cut(s) 869, 877, 1163, 1304, 1979, 2028, 2885, 3014
BseCI ATCGAT 1 cut(s) 1569
BseDI CCNNGG 5 cut(s) 654, 867, 876, 1397, 1977
BseGI GGATG 8 cut(s) 241, 280, 755, 904, 955, 1446, 1651, 2219
BseJI GATNNNNATC 4 cut(s) 1052, 2436, 2442, 3079
BseLI CCNNNNNNNGG 3 cut(s) 1049, 1796, 3013
BseMI GCAATG 1 cut(s) 2211
BseNI ACTGG 4 cut(s) 714, 1012, 2493, 2790
BsePI GCGCGC 1 cut(s) 415
BseSI GKGCMC 3 cut(s) 544, 2185, 2789
BseXI GCAGC 5 cut(s) 925, 1199, 1951, 2018, 2555
BseYI CCCAGC 1 cut(s) 2758
Bsh1236I CGCG 2 cut(s) 137, 417
BshFI GGCC 4 cut(s) 248, 2026, 2582, 2888
BshNI GGYRCC 4 cut(s) 541, 1814, 2370, 2786
BshVI ATCGAT 1 cut(s) 1569
BsiHKAI GWGCWC 2 cut(s) 146, 3265
BsiSI CCGG 2 cut(s) 245, 3373
BslFI GGGAC 3 cut(s) 1959, 2269, 2677
BslI CCNNNNNNNGG 3 cut(s) 1049, 1796, 3013
BsmAI GTCTC 7 cut(s) 534, 553, 576, 777, 1448, 3022, 3250
BsmBI CGTCTC 1 cut(s) 3250
BsmFI GGGAC 3 cut(s) 1959, 2269, 2677
BsmI GAATGC 4 cut(s) 1345, 1603, 1941, 3389
BsnI GGCC 4 cut(s) 248, 2026, 2582, 2888
Bso31I GGTCTC 1 cut(s) 777
Bsp1286I GDGCHC 5 cut(s) 146, 544, 2185, 2789, 3265
Bsp13I TCCGGA 1 cut(s) 3372
Bsp1407I TGTACA 1 cut(s) 2010
Bsp1720I GCTNAGC 2 cut(s) 140, 566
Bsp19I CCATGG 1 cut(s) 1397
BspACI CCGC 3 cut(s) 203, 760, 3291
BspANI GGCC 4 cut(s) 248, 2026, 2582, 2888
BspDI ATCGAT 1 cut(s) 1569
BspEI TCCGGA 1 cut(s) 3372
BspFNI CGCG 2 cut(s) 137, 417
BspHI TCATGA 1 cut(s) 1035
BspLI GGNNCC 9 cut(s) 393, 543, 1491, 1732, 1816, 1975, 2372, 2693, 2788
BspMAI CTGCAG 1 cut(s) 1932
BspMI ACCTGC 2 cut(s) 1785, 2649
BspOI GCTAGC 1 cut(s) 497
BspPI GGATC 9 cut(s) 173, 615, 1018, 1196, 1239, 1725, 1738, 2100, 2445
BspT107I GGYRCC 4 cut(s) 541, 1814, 2370, 2786
BspTI CTTAAG 2 cut(s) 1510, 1615
BspTNI GGTCTC 1 cut(s) 777
BsrDI GCAATG 1 cut(s) 2211
BsrGI TGTACA 1 cut(s) 2010
BsrI ACTGG 4 cut(s) 714, 1012, 2493, 2790
BssECI CCNNGG 5 cut(s) 654, 867, 876, 1397, 1977
BssHII GCGCGC 1 cut(s) 415
BssNAI GTATAC 1 cut(s) 2385
BssNI GRCGYC 1 cut(s) 2371
BssT1I CCWWGG 2 cut(s) 654, 1397
Bst1107I GTATAC 1 cut(s) 2385
Bst2UI CCWGG 8 cut(s) 869, 877, 1163, 1304, 1979, 2028, 2885, 3014
Bst6I CTCTTC 4 cut(s) 2239, 2687, 3072, 3215
BstACI GRCGYC 1 cut(s) 2371
BstAFI CTTAAG 2 cut(s) 1510, 1615
BstAPI GCANNNNNTGC 3 cut(s) 706, 935, 1936
BstAUI TGTACA 1 cut(s) 2010
BstBAI YACGTR 1 cut(s) 1298
BstC8I GCNNGC 8 cut(s) 210, 417, 495, 1093, 1313, 1667, 1819, 2393
BstDSI CCRYGG 1 cut(s) 1397
BstF5I GGATG 8 cut(s) 241, 280, 755, 904, 955, 1446, 1651, 2219
BstFNI CGCG 2 cut(s) 137, 417
BstH2I RGCGCY 1 cut(s) 2374
BstHHI GCGC 5 cut(s) 139, 208, 417, 419, 2373
BstMAI GTCTC 7 cut(s) 534, 553, 576, 777, 1448, 3022, 3250
BstNI CCWGG 8 cut(s) 869, 877, 1163, 1304, 1979, 2028, 2885, 3014
BstNSI RCATGY 2 cut(s) 2395, 2403
BstSCI CCNGG 8 cut(s) 867, 875, 1161, 1302, 1977, 2026, 2883, 3012
BstSFI CTRYAG 2 cut(s) 1438, 1928
BstSLI GKGCMC 3 cut(s) 544, 2185, 2789
BstUI CGCG 2 cut(s) 137, 417
BstV1I GCAGC 5 cut(s) 925, 1199, 1951, 2018, 2555
BstV2I GAAGAC 1 cut(s) 2630
BstX2I RGATCY 7 cut(s) 620, 646, 1010, 1231, 1730, 2092, 2437
BstXI CCANNNNNNTGG 1 cut(s) 1007
BstYI RGATCY 7 cut(s) 620, 646, 1010, 1231, 1730, 2092, 2437
BstZ17I GTATAC 1 cut(s) 2385
Bsu15I ATCGAT 1 cut(s) 1569
BsuI GTATCC 3 cut(s) 1389, 2756, 3317
BsuRI GGCC 4 cut(s) 248, 2026, 2582, 2888
BsuTUI ATCGAT 1 cut(s) 1569
BtgI CCRYGG 1 cut(s) 1397
BtsCI GGATG 8 cut(s) 241, 280, 755, 904, 955, 1446, 1651, 2219
BtsI GCAGTG 3 cut(s) 2162, 2744, 3099
BveI ACCTGC 2 cut(s) 1785, 2649
Cac8I GCNNGC 8 cut(s) 210, 417, 495, 1093, 1313, 1667, 1819, 2393
CciI TCATGA 1 cut(s) 1035
CfoI GCGC 5 cut(s) 139, 208, 417, 419, 2373
Cfr13I GGNCC 4 cut(s) 1973, 2024, 2691, 2814
ClaI ATCGAT 1 cut(s) 1569
CseI GACGC 1 cut(s) 2633
CsiI ACCWGGT 2 cut(s) 1161, 1302
Csp6I GTAC 7 cut(s) 659, 957, 1868, 1905, 2011, 2304, 2403
CspCI CAANNNNNGTGG 2 cut(s) 1073, 1108
CviQI GTAC 7 cut(s) 659, 957, 1868, 1905, 2011, 2304, 2403
DinI GGCGCC 1 cut(s) 2372
DraI TTTAAA 3 cut(s) 675, 1428, 1951
DraIII CACNNNGTG 1 cut(s) 1107
DriI GACNNNNNGTC 1 cut(s) 2197
EaeI YGGCCR 2 cut(s) 246, 2580
Eam1104I CTCTTC 4 cut(s) 2239, 2687, 3072, 3215
Eam1105I GACNNNNNGTC 1 cut(s) 2197
EarI CTCTTC 4 cut(s) 2239, 2687, 3072, 3215
EciI GGCGGA 1 cut(s) 3306
Ecl136II GAGCTC 1 cut(s) 144
Eco130I CCWWGG 2 cut(s) 654, 1397
Eco147I AGGCCT 1 cut(s) 2888
Eco24I GRGCYC 1 cut(s) 146
Eco31I GGTCTC 1 cut(s) 777
Eco32I GATATC 2 cut(s) 2736, 3370
Eco47I GGWCC 3 cut(s) 1973, 2691, 2814
Eco53kI GAGCTC 1 cut(s) 144
Eco57I CTGAAG 1 cut(s) 2484
EcoICRI GAGCTC 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 2691
EcoRII CCWGG 8 cut(s) 867, 875, 1161, 1302, 1977, 2026, 2883, 3012
EcoRV GATATC 2 cut(s) 2736, 3370
EcoT14I CCWWGG 2 cut(s) 654, 1397
EcoT22I ATGCAT 1 cut(s) 3389
EcoT38I GRGCYC 1 cut(s) 146
EgeI GGCGCC 1 cut(s) 2372
EheI GGCGCC 1 cut(s) 2372
ErhI CCWWGG 2 cut(s) 654, 1397
Esp3I CGTCTC 1 cut(s) 3250
FaqI GGGAC 3 cut(s) 1959, 2269, 2677
FbaI TGATCA 3 cut(s) 1032, 1155, 2794
FblI GTMKAC 2 cut(s) 1170, 2384
Fnu4HI GCNGC 6 cut(s) 204, 939, 1188, 1940, 2032, 2569
FokI GGATG 8 cut(s) 228, 287, 762, 891, 962, 1433, 1658, 2226
FriOI GRGCYC 1 cut(s) 146
Fsp4HI GCNGC 6 cut(s) 204, 939, 1188, 1940, 2032, 2569
FspBI CTAG 7 cut(s) 494, 767, 1227, 2388, 2553, 2652, 3161
GlaI GCGC 5 cut(s) 138, 207, 416, 418, 2372
GluI GCNGC 6 cut(s) 204, 939, 1188, 1940, 2032, 2569
GsaI CCCAGC 1 cut(s) 2762
GsuI CTGGAG 1 cut(s) 3046
HaeII RGCGCY 1 cut(s) 2374
HaeIII GGCC 4 cut(s) 248, 2026, 2582, 2888
HapII CCGG 2 cut(s) 245, 3373
HgaI GACGC 1 cut(s) 2633
HhaI GCGC 5 cut(s) 139, 208, 417, 419, 2373
Hin1I GRCGYC 1 cut(s) 2371
Hin6I GCGC 5 cut(s) 137, 206, 415, 417, 2371
HinP1I GCGC 5 cut(s) 137, 206, 415, 417, 2371
HincII GTYRAC 3 cut(s) 459, 1454, 1896
HindII GTYRAC 3 cut(s) 459, 1454, 1896
HindIII AAGCTT 1 cut(s) 2528
HpaII CCGG 2 cut(s) 245, 3373
HphI GGTGA 4 cut(s) 42, 1004, 1720, 2938
Hpy166II GTNNAC 9 cut(s) 380, 459, 1084, 1171, 1277, 1454, 1896, 2385, 2744
Hpy8I GTNNAC 9 cut(s) 380, 459, 1084, 1171, 1277, 1454, 1896, 2385, 2744
Hpy99I CGWCG 3 cut(s) 173, 191, 194
HpyCH4IV ACGT 3 cut(s) 1297, 1794, 1907
HpySE526I ACGT 3 cut(s) 1297, 1794, 1907
Hsp92I GRCGYC 1 cut(s) 2371
HspAI GCGC 5 cut(s) 137, 206, 415, 417, 2371
KasI GGCGCC 1 cut(s) 2370
Kpn2I TCCGGA 1 cut(s) 3372
Ksp22I TGATCA 3 cut(s) 1032, 1155, 2794
LmnI GCTCC 8 cut(s) 27, 397, 1348, 1510, 2180, 2222, 3009, 3109
Lsp1109I GCAGC 5 cut(s) 925, 1199, 1951, 2018, 2555
LweI GCATC 9 cut(s) 106, 687, 883, 1062, 1492, 1678, 2204, 2455, 3250
MabI ACCWGGT 2 cut(s) 1161, 1302
MaeI CTAG 7 cut(s) 494, 767, 1227, 2388, 2553, 2652, 3161
MaeII ACGT 3 cut(s) 1297, 1794, 1907
MaeIII GTNAC 5 cut(s) 69, 992, 1064, 1298, 1652
MfeI CAATTG 1 cut(s) 2631
MflI RGATCY 7 cut(s) 620, 646, 1010, 1231, 1730, 2092, 2437
MhlI GDGCHC 5 cut(s) 146, 544, 2185, 2789, 3265
MlsI TGGCCA 1 cut(s) 2582
MluNI TGGCCA 1 cut(s) 2582
Mly113I GGCGCC 1 cut(s) 2371
MlyI GAGTC 2 cut(s) 839, 2108
MmeI TCCRAC 4 cut(s) 143, 1395, 1777, 3207
Mox20I TGGCCA 1 cut(s) 2582
Mph1103I ATGCAT 1 cut(s) 3389
MroI TCCGGA 1 cut(s) 3372
MscI TGGCCA 1 cut(s) 2582
MslI CAYNNNNRTG 4 cut(s) 217, 695, 748, 1883
Msp20I TGGCCA 1 cut(s) 2582
MspA1I CMGCKG 2 cut(s) 373, 1496
MspCI CTTAAG 2 cut(s) 1510, 1615
MspI CCGG 2 cut(s) 245, 3373
MspR9I CCNGG 8 cut(s) 869, 877, 1163, 1304, 1979, 2028, 2885, 3014
MunI CAATTG 1 cut(s) 2631
Mva1269I GAATGC 4 cut(s) 1345, 1603, 1941, 3389
MvaI CCWGG 8 cut(s) 869, 877, 1163, 1304, 1979, 2028, 2885, 3014
MvnI CGCG 2 cut(s) 137, 417
NarI GGCGCC 1 cut(s) 2371
NcoI CCATGG 1 cut(s) 1397
NheI GCTAGC 1 cut(s) 493
NlaIV GGNNCC 9 cut(s) 393, 543, 1491, 1732, 1816, 1975, 2372, 2693, 2788
NmuCI GTSAC 4 cut(s) 69, 992, 1064, 1652
NsiI ATGCAT 1 cut(s) 3389
NspI RCATGY 2 cut(s) 2395, 2403
OliI CACNNNNGTG 1 cut(s) 1883
PacI TTAATTAA 1 cut(s) 801
PaeI GCATGC 1 cut(s) 2395
PagI TCATGA 1 cut(s) 1035
PauI GCGCGC 1 cut(s) 415
PceI AGGCCT 1 cut(s) 2888
PciI ACATGT 1 cut(s) 2399
PcsI WCGNNNNNNNCGW 1 cut(s) 3265
PctI GAATGC 4 cut(s) 1345, 1603, 1941, 3389
PflFI GACNNNGTC 1 cut(s) 2841
PflMI CCANNNNNTGG 1 cut(s) 3013
PfoI TCCNGGA 1 cut(s) 3012
PkrI GCNGC 6 cut(s) 205, 940, 1189, 1941, 2033, 2570
PleI GAGTC 2 cut(s) 839, 2108
PluTI GGCGCC 1 cut(s) 2374
PpsI GAGTC 2 cut(s) 839, 2108
Ppu21I YACGTR 1 cut(s) 1298
PpuMI RGGWCCY 1 cut(s) 2691
PscI ACATGT 1 cut(s) 2399
PshBI ATTAAT 2 cut(s) 801, 1242
Psp124BI GAGCTC 1 cut(s) 146
Psp5II RGGWCCY 1 cut(s) 2691
Psp6I CCWGG 8 cut(s) 867, 875, 1161, 1302, 1977, 2026, 2883, 3012
PspFI CCCAGC 1 cut(s) 2758
PspGI CCWGG 8 cut(s) 867, 875, 1161, 1302, 1977, 2026, 2883, 3012
PspN4I GGNNCC 9 cut(s) 393, 543, 1491, 1732, 1816, 1975, 2372, 2693, 2788
PspPI GGNCC 4 cut(s) 1973, 2024, 2691, 2814
PspPPI RGGWCCY 1 cut(s) 2691
PstI CTGCAG 1 cut(s) 1932
PsuI RGATCY 7 cut(s) 620, 646, 1010, 1231, 1730, 2092, 2437
PsyI GACNNNGTC 1 cut(s) 2841
PteI GCGCGC 1 cut(s) 415
PvuII CAGCTG 2 cut(s) 373, 1496
RsaI GTAC 7 cut(s) 660, 958, 1869, 1906, 2012, 2305, 2404
RsaNI GTAC 7 cut(s) 659, 957, 1868, 1905, 2011, 2304, 2403
RseI CAYNNNNRTG 4 cut(s) 217, 695, 748, 1883
SacI GAGCTC 1 cut(s) 146
SatI GCNGC 6 cut(s) 204, 939, 1188, 1940, 2032, 2569
Sau96I GGNCC 4 cut(s) 1973, 2024, 2691, 2814
SchI GAGTC 2 cut(s) 839, 2108
ScrFI CCNGG 8 cut(s) 869, 877, 1163, 1304, 1979, 2028, 2885, 3014
SduI GDGCHC 5 cut(s) 146, 544, 2185, 2789, 3265
SexAI ACCWGGT 2 cut(s) 1161, 1302
SfaNI GCATC 9 cut(s) 106, 687, 883, 1062, 1492, 1678, 2204, 2455, 3250
SfcI CTRYAG 2 cut(s) 1438, 1928
SfoI GGCGCC 1 cut(s) 2372
SinI GGWCC 3 cut(s) 1973, 2691, 2814
SmiMI CAYNNNNRTG 4 cut(s) 217, 695, 748, 1883
SmlI CTYRAG 5 cut(s) 1281, 1510, 1615, 2750, 2897
SmoI CTYRAG 5 cut(s) 1281, 1510, 1615, 2750, 2897
SpeI ACTAGT 2 cut(s) 2552, 3160
SphI GCATGC 1 cut(s) 2395
SseBI AGGCCT 1 cut(s) 2888
SsiI CCGC 3 cut(s) 203, 760, 3291
SspDI GGCGCC 1 cut(s) 2370
SspI AATATT 1 cut(s) 1946
SspMI CTAG 7 cut(s) 494, 767, 1227, 2388, 2553, 2652, 3161
SstI GAGCTC 1 cut(s) 146
StuI AGGCCT 1 cut(s) 2888
StyD4I CCNGG 8 cut(s) 867, 875, 1161, 1302, 1977, 2026, 2883, 3012
StyI CCWWGG 2 cut(s) 654, 1397
TaiI ACGT 3 cut(s) 1300, 1797, 1910
TaqI TCGA 4 cut(s) 5, 1569, 3206, 3327
TaqII GACCGA 2 cut(s) 1881, 2831
TatI WGTACW 2 cut(s) 2010, 2402
TauI GCSGC 1 cut(s) 206
TseFI GTSAC 4 cut(s) 69, 992, 1064, 1652
TseI GCWGC 5 cut(s) 938, 1187, 1939, 2031, 2568
Tsp45I GTSAC 4 cut(s) 69, 992, 1064, 1652
TspGWI ACGGA 2 cut(s) 149, 1439
Tth111I GACNNNGTC 1 cut(s) 2841
Van91I CCANNNNNTGG 1 cut(s) 3013
Vha464I CTTAAG 2 cut(s) 1510, 1615
VpaK11BI GGWCC 3 cut(s) 1973, 2691, 2814
VspI ATTAAT 2 cut(s) 801, 1242
XapI RAATTY 5 cut(s) 261, 816, 1952, 2488, 2879
XceI RCATGY 2 cut(s) 2395, 2403
XcmI CCANNNNNNNNNTGG 1 cut(s) 2751
XmiI GTMKAC 2 cut(s) 1170, 2384
XspI CTAG 7 cut(s) 494, 767, 1227, 2388, 2553, 2652, 3161
Zsp2I ATGCAT 1 cut(s) 3389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.