pycom15g14920

Rap1-interacting factor 1 N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006378
Physical Location & Seq
Reverse (-)
10297848 .. 10299109
1262 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g14920.1

Sequence Viewer

Length: 1077 bp
ATGCACCACATGCTAACCAGTGGCTTGAAGGTTCAAACTATTCAAGCATGGGGATCGTTTATCCACTTACTGGGATCTCGTGCCTTGAAGAACGGGCAGTTAATTAATCAGATGCTAAAAATTCCCGAGCAAACATTTTCAGATCATGACTCACAAGTTCAAATTGCTTCACAAGTTGCCTGGGAAGGTCTTATTGATGCCCTTGTTCACCCTACCATGATATTACCCTTTGAGACAAATGTGACAAAAAAGGACAATGGTATGGAACAAATAGGAACATGCAAAGGAAACGGTAGTGAAATTCAAAAAAAGGGATACTTGAAAAGCACAAAGCTCATTATGACGCCACTGATAGGCATCATGCTGACTAAATGTGATGTATCTGTTCACTTAGCTTGCTTGAACACGTGGTGTTATTTATTGCATAAACTTGATACCTCTCTCAGTGATTCTTCTATCATCAGAATGGTACTAGAGCCAGTTGTTGAGGCAGTTTTTCAAGTTGATCCTGATAGTAAGAGCACCTGGGCCAGGAATCTGTGCATAGATTTGCTTAATGTTTTCATATTGGCAAAATGTAAAGATATAGATTATGACTCACTTAATCAAGTGAACCATCAGTTATTGCGCAAAACCTCTAGGATTGCTCCCTTTGTATCTGGAAGTTTCTCTTGGAAGCAGTGTGCCATCAAATGGTTTCCATGGGATCTGAGTCTTTTAGACTTCCATTTAAAGATGATCTACATTCTTATCTGTCAAGCGTCAAGGGAAACTGTCTCCCACGATAACAGGATTTCAGCTGCGGATGCCTCCTTAAGGTTATTTAGATCTGTTTTCAAAGGAGTTCAGTTGGAGTTGAAAAGGTCGTCTATCAGTTATGATGATATTATGTTTTGTTTGAATGCAATATTGAAGTTTGTAAAAAACACTTGTGAAAAAGTTATTTCAGATTGTAGTGACAGAAATGACTTACATCACATATCGCTTCAGTTGGTGGAGGCTGTCTGTGCGGAGATTGAACATACTATAGTGGGATTCCCTATTTACAGGGTGCCATTTCACCAATGGTTTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04550 map04550
Pfam Domains
Protein Families
Rif1_N

Protein Analysis

359

Amino Acids

40.68

Weight (kDa)

7.59

Isoelectric Point (pI)

29.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rif1_N PF12231 3 - 68 5.9e-10 Rap1-interacting factor 1 N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 629
AccB1I GGYRCC 1 cut(s) 1051
AccB7I CCANNNNNTGG 2 cut(s) 70, 693
AciI CCGC 2 cut(s) 803, 1010
AclWI GGATC 4 cut(s) 61, 82, 500, 714
AcsI RAATTY 2 cut(s) 120, 300
AcuI CTGAAG 1 cut(s) 971
AcvI CACGTG 1 cut(s) 408
AcyI GRCGYC 1 cut(s) 344
AdeI CACNNNGTG 1 cut(s) 411
AfaI GTAC 1 cut(s) 471
AfiI CCNNNNNNNGG 5 cut(s) 70, 353, 531, 693, 816
AflII CTTAAG 1 cut(s) 814
AflIII ACRYGT 1 cut(s) 405
AjnI CCWGG 3 cut(s) 179, 524, 530
AjuI GAANNNNNNNTTGG 2 cut(s) 655, 687
AluBI AGCT 3 cut(s) 334, 395, 800
AluI AGCT 3 cut(s) 334, 395, 800
Alw21I GWGCWC 1 cut(s) 524
Alw26I GTCTC 2 cut(s) 227, 781
AlwI GGATC 4 cut(s) 61, 82, 500, 714
Ama87I CYCGRG 1 cut(s) 125
AoxI GGCC 1 cut(s) 528
ApeKI GCWGC 1 cut(s) 800
ApoI RAATTY 2 cut(s) 120, 300
AseI ATTAAT 1 cut(s) 105
AspLEI GCGC 1 cut(s) 630
AspS9I GGNCC 1 cut(s) 528
AsuHPI GGTGA 2 cut(s) 200, 1052
AvaI CYCGRG 1 cut(s) 125
BanI GGYRCC 1 cut(s) 1051
BauI CACGAG 1 cut(s) 78
BbrPI CACGTG 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 524
BbvI GCAGC 1 cut(s) 787
BccI CCATC 2 cut(s) 624, 695
BcgI CGANNNNNNTGC 2 cut(s) 36, 70
BciT130I CCWGG 3 cut(s) 181, 526, 532
BciVI GTATCC 1 cut(s) 308
BcoDI GTCTC 2 cut(s) 227, 781
BfaI CTAG 2 cut(s) 473, 639
BfmI CTRYAG 1 cut(s) 1026
BfrI CTTAAG 1 cut(s) 814
BfuI GTATCC 1 cut(s) 308
BglII AGATCT 1 cut(s) 827
BisI GCNGC 1 cut(s) 801
BlsI GCNGC 1 cut(s) 802
Bme1390I CCNGG 3 cut(s) 181, 526, 532
BmeT110I CYCGRG 1 cut(s) 125
BmgT120I GGNCC 1 cut(s) 528
BmiI GGNNCC 1 cut(s) 1053
BmrFI CCNGG 3 cut(s) 181, 526, 532
BmrI ACTGGG 1 cut(s) 80
BmsI GCATC 4 cut(s) 102, 187, 366, 796
BmuI ACTGGG 1 cut(s) 80
BsaAI YACGTR 1 cut(s) 408
BsaBI GATNNNNATC 1 cut(s) 356
BsaHI GRCGYC 1 cut(s) 344
BsaJI CCNNGG 3 cut(s) 180, 525, 701
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 353, 531, 693, 816
Bse1I ACTGG 3 cut(s) 18, 75, 479
Bse8I GATNNNNATC 1 cut(s) 356
BseBI CCWGG 3 cut(s) 181, 526, 532
BseDI CCNNGG 3 cut(s) 180, 525, 701
BseGI GGATG 1 cut(s) 811
BseJI GATNNNNATC 1 cut(s) 356
BseLI CCNNNNNNNGG 5 cut(s) 70, 353, 531, 693, 816
BseMII CTCAG 2 cut(s) 457, 701
BseNI ACTGG 3 cut(s) 18, 75, 479
BseXI GCAGC 1 cut(s) 787
BshFI GGCC 1 cut(s) 530
BshNI GGYRCC 1 cut(s) 1051
BsiHKAI GWGCWC 1 cut(s) 524
BsiHKCI CYCGRG 1 cut(s) 125
BslI CCNNNNNNNGG 5 cut(s) 70, 353, 531, 693, 816
BsmAI GTCTC 2 cut(s) 227, 781
BsmI GAATGC 1 cut(s) 907
BsnI GGCC 1 cut(s) 530
BsoBI CYCGRG 1 cut(s) 125
Bsp1286I GDGCHC 1 cut(s) 524
Bsp143I GATC 7 cut(s) 53, 74, 142, 505, 706, 738, 827
Bsp19I CCATGG 1 cut(s) 701
BspACI CCGC 2 cut(s) 803, 1010
BspANI GGCC 1 cut(s) 530
BspCNI CTCAG 2 cut(s) 456, 702
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 1 cut(s) 1053
BspPI GGATC 4 cut(s) 61, 82, 500, 714
BspT107I GGYRCC 1 cut(s) 1051
BspTI CTTAAG 1 cut(s) 814
BsrI ACTGG 3 cut(s) 18, 75, 479
BssECI CCNNGG 3 cut(s) 180, 525, 701
BssMI GATC 7 cut(s) 53, 74, 142, 505, 706, 738, 827
BssNI GRCGYC 1 cut(s) 344
BssSI CACGAG 1 cut(s) 78
BssT1I CCWWGG 1 cut(s) 701
Bst2BI CACGAG 1 cut(s) 78
Bst2UI CCWGG 3 cut(s) 181, 526, 532
Bst4CI ACNGT 2 cut(s) 293, 775
BstACI GRCGYC 1 cut(s) 344
BstAFI CTTAAG 1 cut(s) 814
BstAPI GCANNNNNTGC 1 cut(s) 10
BstBAI YACGTR 1 cut(s) 408
BstC8I GCNNGC 1 cut(s) 397
BstDEI CTNAG 3 cut(s) 391, 443, 710
BstDSI CCRYGG 1 cut(s) 701
BstENI CCTNNNNNAGG 2 cut(s) 529, 814
BstF5I GGATG 1 cut(s) 811
BstHHI GCGC 1 cut(s) 630
BstKTI GATC 7 cut(s) 56, 77, 145, 508, 709, 741, 830
BstMAI GTCTC 2 cut(s) 227, 781
BstMBI GATC 7 cut(s) 53, 74, 142, 505, 706, 738, 827
BstMWI GCNNNNNNNGC 3 cut(s) 10, 806, 1007
BstNI CCWGG 3 cut(s) 181, 526, 532
BstNSI RCATGY 2 cut(s) 13, 282
BstSCI CCNGG 3 cut(s) 179, 524, 530
BstSFI CTRYAG 1 cut(s) 1026
BstV1I GCAGC 1 cut(s) 787
BstX2I RGATCY 3 cut(s) 74, 706, 827
BstYI RGATCY 3 cut(s) 74, 706, 827
BsuI GTATCC 1 cut(s) 308
BsuRI GGCC 1 cut(s) 530
BtgI CCRYGG 1 cut(s) 701
BtsCI GGATG 1 cut(s) 811
BtsI GCAGTG 1 cut(s) 686
BtsIMutI CAGTG 4 cut(s) 25, 347, 451, 686
Cac8I GCNNGC 1 cut(s) 397
CciI TCATGA 1 cut(s) 145
CfoI GCGC 1 cut(s) 630
Cfr13I GGNCC 1 cut(s) 528
CseI GACGC 2 cut(s) 352, 750
Csp6I GTAC 1 cut(s) 470
CviAII CATG 7 cut(s) 10, 48, 146, 217, 279, 361, 702
CviJI RGCY 7 cut(s) 24, 334, 395, 478, 530, 800, 1001
CviKI_1 RGCY 7 cut(s) 24, 334, 395, 478, 530, 800, 1001
CviQI GTAC 1 cut(s) 470
DdeI CTNAG 3 cut(s) 391, 443, 710
DpnI GATC 7 cut(s) 55, 76, 144, 507, 708, 740, 829
DpnII GATC 7 cut(s) 53, 74, 142, 505, 706, 738, 827
DraI TTTAAA 1 cut(s) 732
DraIII CACNNNGTG 1 cut(s) 411
Eco130I CCWWGG 1 cut(s) 701
Eco57I CTGAAG 1 cut(s) 971
Eco72I CACGTG 1 cut(s) 408
Eco88I CYCGRG 1 cut(s) 125
EcoNI CCTNNNNNAGG 2 cut(s) 529, 814
EcoRII CCWGG 3 cut(s) 179, 524, 530
EcoT14I CCWWGG 1 cut(s) 701
ErhI CCWWGG 1 cut(s) 701
FaeI CATG 7 cut(s) 13, 51, 149, 220, 282, 364, 705
FalI AAGNNNNNCTT 4 cut(s) 302, 334, 655, 687
FatI CATG 7 cut(s) 9, 47, 145, 216, 278, 360, 701
Fnu4HI GCNGC 1 cut(s) 801
FokI GGATG 1 cut(s) 818
Fsp4HI GCNGC 1 cut(s) 801
FspBI CTAG 2 cut(s) 473, 639
FspI TGCGCA 1 cut(s) 629
GlaI GCGC 1 cut(s) 629
GluI GCNGC 1 cut(s) 801
HaeIII GGCC 1 cut(s) 530
HgaI GACGC 2 cut(s) 352, 750
HhaI GCGC 1 cut(s) 630
Hin1I GRCGYC 1 cut(s) 344
Hin1II CATG 7 cut(s) 13, 51, 149, 220, 282, 364, 705
Hin6I GCGC 1 cut(s) 628
HinP1I GCGC 1 cut(s) 628
HinfI GANTC 6 cut(s) 149, 449, 535, 596, 712, 1035
HphI GGTGA 2 cut(s) 200, 1052
Hpy166II GTNNAC 3 cut(s) 208, 388, 613
Hpy188I TCNGA 5 cut(s) 111, 142, 464, 711, 949
Hpy188III TCNNGA 4 cut(s) 125, 146, 509, 660
Hpy8I GTNNAC 3 cut(s) 208, 388, 613
HpyAV CCTTC 2 cut(s) 22, 179
HpyCH4III ACNGT 2 cut(s) 293, 775
HpyCH4IV ACGT 1 cut(s) 407
HpyCH4V TGCA 5 cut(s) 4, 282, 424, 543, 905
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 806, 1007
HpyF3I CTNAG 3 cut(s) 391, 443, 710
HpySE526I ACGT 1 cut(s) 407
Hsp92I GRCGYC 1 cut(s) 344
Hsp92II CATG 7 cut(s) 13, 51, 149, 220, 282, 364, 705
HspAI GCGC 1 cut(s) 628
Kzo9I GATC 7 cut(s) 53, 74, 142, 505, 706, 738, 827
LmnI GCTCC 1 cut(s) 652
Lsp1109I GCAGC 1 cut(s) 787
LweI GCATC 4 cut(s) 102, 187, 366, 796
MaeI CTAG 2 cut(s) 473, 639
MaeII ACGT 1 cut(s) 407
MaeIII GTNAC 2 cut(s) 241, 956
MalI GATC 7 cut(s) 55, 76, 144, 507, 708, 740, 829
MboI GATC 7 cut(s) 53, 74, 142, 505, 706, 738, 827
MboII GAAGA 2 cut(s) 100, 444
MflI RGATCY 3 cut(s) 74, 706, 827
MhlI GDGCHC 1 cut(s) 524
MluCI AATT 4 cut(s) 102, 120, 162, 300
MlyI GAGTC 3 cut(s) 143, 590, 721
MmeI TCCRAC 1 cut(s) 831
MnlI CCTC 5 cut(s) 448, 481, 646, 820, 991
MseI TTAA 7 cut(s) 101, 105, 555, 603, 731, 815, 1075
MslI CAYNNNNRTG 1 cut(s) 464
MspA1I CMGCKG 1 cut(s) 800
MspCI CTTAAG 1 cut(s) 814
MspR9I CCNGG 3 cut(s) 181, 526, 532
Mva1269I GAATGC 1 cut(s) 907
MvaI CCWGG 3 cut(s) 181, 526, 532
MwoI GCNNNNNNNGC 3 cut(s) 10, 806, 1007
NcoI CCATGG 1 cut(s) 701
NdeII GATC 7 cut(s) 53, 74, 142, 505, 706, 738, 827
NlaIII CATG 7 cut(s) 13, 51, 149, 220, 282, 364, 705
NlaIV GGNNCC 1 cut(s) 1053
NmuCI GTSAC 2 cut(s) 241, 956
NsbI TGCGCA 1 cut(s) 629
NspI RCATGY 2 cut(s) 13, 282
PacI TTAATTAA 1 cut(s) 105
PagI TCATGA 1 cut(s) 145
PctI GAATGC 1 cut(s) 907
PfeI GAWTC 3 cut(s) 449, 535, 1035
PflMI CCANNNNNTGG 2 cut(s) 70, 693
PkrI GCNGC 1 cut(s) 802
PleI GAGTC 3 cut(s) 143, 590, 720
PmaCI CACGTG 1 cut(s) 408
PmlI CACGTG 1 cut(s) 408
PpsI GAGTC 3 cut(s) 143, 590, 720
Ppu21I YACGTR 1 cut(s) 408
PshBI ATTAAT 1 cut(s) 105
Psp6I CCWGG 3 cut(s) 179, 524, 530
PspCI CACGTG 1 cut(s) 408
PspGI CCWGG 3 cut(s) 179, 524, 530
PspN4I GGNNCC 1 cut(s) 1053
PspPI GGNCC 1 cut(s) 528
PsuI RGATCY 3 cut(s) 74, 706, 827
PvuII CAGCTG 1 cut(s) 800
RsaI GTAC 1 cut(s) 471
RsaNI GTAC 1 cut(s) 470
RseI CAYNNNNRTG 1 cut(s) 464
SaqAI TTAA 7 cut(s) 101, 105, 555, 603, 731, 815, 1075
SatI GCNGC 1 cut(s) 801
Sau3AI GATC 7 cut(s) 53, 74, 142, 505, 706, 738, 827
Sau96I GGNCC 1 cut(s) 528
SchI GAGTC 3 cut(s) 143, 590, 721
ScrFI CCNGG 3 cut(s) 181, 526, 532
SduI GDGCHC 1 cut(s) 524
SfaNI GCATC 4 cut(s) 102, 187, 366, 796
SfcI CTRYAG 1 cut(s) 1026
SmiMI CAYNNNNRTG 1 cut(s) 464
SmlI CTYRAG 1 cut(s) 814
SmoI CTYRAG 1 cut(s) 814
Sse9I AATT 4 cut(s) 102, 120, 162, 300
SsiI CCGC 2 cut(s) 803, 1010
SspI AATATT 1 cut(s) 909
SspMI CTAG 2 cut(s) 473, 639
StyD4I CCNGG 3 cut(s) 179, 524, 530
StyI CCWWGG 1 cut(s) 701
TaaI ACNGT 2 cut(s) 293, 775
TaiI ACGT 1 cut(s) 410
TasI AATT 4 cut(s) 102, 120, 162, 300
TfiI GAWTC 3 cut(s) 449, 535, 1035
Tru1I TTAA 7 cut(s) 101, 105, 555, 603, 731, 815, 1075
Tru9I TTAA 7 cut(s) 101, 105, 555, 603, 731, 815, 1075
TscAI CASTG 4 cut(s) 25, 354, 451, 686
TseFI GTSAC 2 cut(s) 241, 956
TseI GCWGC 1 cut(s) 800
Tsp45I GTSAC 2 cut(s) 241, 956
TspDTI ATGAA 1 cut(s) 553
TspRI CASTG 4 cut(s) 25, 354, 451, 686
Van91I CCANNNNNTGG 2 cut(s) 70, 693
Vha464I CTTAAG 1 cut(s) 814
VspI ATTAAT 1 cut(s) 105
XagI CCTNNNNNAGG 2 cut(s) 529, 814
XapI RAATTY 2 cut(s) 120, 300
XceI RCATGY 2 cut(s) 13, 282
XcmI CCANNNNNNNNNTGG 1 cut(s) 1062
XspI CTAG 2 cut(s) 473, 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.